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The map label for this gene is ramA [H]

Identifier: 238024882

GI number: 238024882

Start: 1930144

End: 1930983

Strand: Reverse

Name: ramA [H]

Synonym: bglu_2g15230

Alternate gene names: 238024882

Gene position: 1930983-1930144 (Counterclockwise)

Preceding gene: 238024885

Following gene: 238024881

Centisome position: 68.3

GC content: 71.43

Gene sequence:

>840_bases
ATGCAAATCGAACTCGCCCAGATTCCGGTCGCCGACGGCAAGATCGCACCGAATCTGGCCCGCGTGACCGAGGTGATCCG
CGCGCGGCGCGACGGCACCGACCTGATCGTCTTCCCCGAGACCACGCTGACGGGCTTCCCGACGCGCGAGAGCGTGCGCG
ACGTGGCCGAGGCGCTCGACGGCCCGTCGGTCGCCGCGGTGCGCGAGGCGGCCCGCGCGGCCGGCGTGGGCGTGGCGGTG
GGCTTCGCGGAGCGCGACGGCGGCCGCTTCTTCAACACCACGCTGCTGATCGACGCGCATGGCGAGATCGCGCTGCGCTA
CCGGAAAACGCACCTCTGGGCGTCCGACGTCGGCGTGTTCGAGCCCGGCGATCGCTACGCCGTCTGCGAATTCAAGGGCA
TGACGGTGGGCCTCTTGATCTGCTACGACATCGAGTTCCCGGAGACTGCGCGCGCCATCGCCTCGCTCGGTGCCGACCTG
CTGATCGTCACCAACGGCAACATGGACCCGTTCGGCCCCGTGCATCGCCGCGCGATCGTCGCGCGCGCGATGGAGAACCA
GATGTTCGCCGCGCTCGTGAACCGGATCGGCGACGGCGACGACAACCTGACCTTCCCCGGCGAGTCGGCGCTGATCGGCC
CCTCGGGCGACGTGCTGGGCGAGCTGCGCGGCGAGGACGCGGTGCTCTGCGTGAGGCTCGATCCGACGCTGCTGGCCGCG
AGCCGCGAGCACTACAGCTATCTGCACGACGCGCGGGTGGGCCTCGTGCTGCACGACGATCCGCAGGACGCCGCGGCCGC
GCCGTTCGACGGCCGCGGCCGCGTGATCCGCGCGCGCTGA

Upstream 100 bases:

>100_bases
TGGACAAAAATAACGTCCCGATAATAGCGCCACGCCGCCGCCCGAGCGGCCGGCGAAATGCGAGCGCGAGTCTATCGGCC
CGCCATACAGGAGGCAAGCA

Downstream 100 bases:

>100_bases
GCGGATCGTCATTTCGTTTGCCGCTGGTTGTTTTTGCCGTTCGTTTTGCGCTGTCCGTTTTCGCCGTAACCACCCATGAT
CCCCATGCCGCCACCGCGGC

Product: nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MQIELAQIPVADGKIAPNLARVTEVIRARRDGTDLIVFPETTLTGFPTRESVRDVAEALDGPSVAAVREAARAAGVGVAV
GFAERDGGRFFNTTLLIDAHGEIALRYRKTHLWASDVGVFEPGDRYAVCEFKGMTVGLLICYDIEFPETARAIASLGADL
LIVTNGNMDPFGPVHRRAIVARAMENQMFAALVNRIGDGDDNLTFPGESALIGPSGDVLGELRGEDAVLCVRLDPTLLAA
SREHYSYLHDARVGLVLHDDPQDAAAAPFDGRGRVIRAR

Sequences:

>Translated_279_residues
MQIELAQIPVADGKIAPNLARVTEVIRARRDGTDLIVFPETTLTGFPTRESVRDVAEALDGPSVAAVREAARAAGVGVAV
GFAERDGGRFFNTTLLIDAHGEIALRYRKTHLWASDVGVFEPGDRYAVCEFKGMTVGLLICYDIEFPETARAIASLGADL
LIVTNGNMDPFGPVHRRAIVARAMENQMFAALVNRIGDGDDNLTFPGESALIGPSGDVLGELRGEDAVLCVRLDPTLLAA
SREHYSYLHDARVGLVLHDDPQDAAAAPFDGRGRVIRAR
>Mature_279_residues
MQIELAQIPVADGKIAPNLARVTEVIRARRDGTDLIVFPETTLTGFPTRESVRDVAEALDGPSVAAVREAARAAGVGVAV
GFAERDGGRFFNTTLLIDAHGEIALRYRKTHLWASDVGVFEPGDRYAVCEFKGMTVGLLICYDIEFPETARAIASLGADL
LIVTNGNMDPFGPVHRRAIVARAMENQMFAALVNRIGDGDDNLTFPGESALIGPSGDVLGELRGEDAVLCVRLDPTLLAA
SREHYSYLHDARVGLVLHDDPQDAAAAPFDGRGRVIRAR

Specific function: Hydrolyzes (R)-piperazine-2-carboxamide and (R)- piperazine-2-tert-butylcarboxamide with strict R- stereoselectivity. Also active towards beta-alaninamide, piperidine-3-carboxmide, D-glutaminamide and slightly active towards L-glutaminamide and piperidine

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=267, Percent_Identity=26.5917602996255, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI297632350, Length=260, Percent_Identity=26.5384615384615, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI5031947, Length=260, Percent_Identity=26.5384615384615, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI297632348, Length=260, Percent_Identity=26.5384615384615, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1786412, Length=219, Percent_Identity=28.310502283105, Blast_Score=75, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6323383, Length=263, Percent_Identity=23.1939163498099, Blast_Score=76, Evalue=7e-15,
Organism=Drosophila melanogaster, GI17933642, Length=225, Percent_Identity=29.3333333333333, Blast_Score=94, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.100 [H]

Molecular weight: Translated: 30004; Mature: 30004

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIELAQIPVADGKIAPNLARVTEVIRARRDGTDLIVFPETTLTGFPTRESVRDVAEALD
CEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCC
GPSVAAVREAARAAGVGVAVGFAERDGGRFFNTTLLIDAHGEIALRYRKTHLWASDVGVF
CCCHHHHHHHHHHCCCEEEEEEEECCCCEEEEEEEEEECCCCEEEEEEEEEEEECCCCCC
EPGDRYAVCEFKGMTVGLLICYDIEFPETARAIASLGADLLIVTNGNMDPFGPVHRRAIV
CCCCCEEEEEECCCEEEEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
ARAMENQMFAALVNRIGDGDDNLTFPGESALIGPSGDVLGELRGEDAVLCVRLDPTLLAA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCHHHHCCCCCEEEEEEECCHHEEC
SREHYSYLHDARVGLVLHDDPQDAAAAPFDGRGRVIRAR
CHHHHHHHHHCEEEEEEECCCCCCCCCCCCCCCCEEEEC
>Mature Secondary Structure
MQIELAQIPVADGKIAPNLARVTEVIRARRDGTDLIVFPETTLTGFPTRESVRDVAEALD
CEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHCC
GPSVAAVREAARAAGVGVAVGFAERDGGRFFNTTLLIDAHGEIALRYRKTHLWASDVGVF
CCCHHHHHHHHHHCCCEEEEEEEECCCCEEEEEEEEEECCCCEEEEEEEEEEEECCCCCC
EPGDRYAVCEFKGMTVGLLICYDIEFPETARAIASLGADLLIVTNGNMDPFGPVHRRAIV
CCCCCEEEEEECCCEEEEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHH
ARAMENQMFAALVNRIGDGDDNLTFPGESALIGPSGDVLGELRGEDAVLCVRLDPTLLAA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCHHHHCCCCCEEEEEEECCHHEEC
SREHYSYLHDARVGLVLHDDPQDAAAAPFDGRGRVIRAR
CHHHHHHHHHCEEEEEEECCCCCCCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA