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The map label for this gene is nemA [H]

Identifier: 238024825

GI number: 238024825

Start: 1861689

End: 1862750

Strand: Direct

Name: nemA [H]

Synonym: bglu_2g14630

Alternate gene names: 238024825

Gene position: 1861689-1862750 (Clockwise)

Preceding gene: 238024824

Following gene: 238024827

Centisome position: 65.85

GC content: 70.15

Gene sequence:

>1062_bases
ATGCCTACCCTCTTTGATCCGCTAGTCATCGGCGACCTGACGCTGCGCAACCGCGTCGTGATGGCCCCGCTCACGCGCGC
CCGCGCCAACCAGGCCCGCGTGCCGAACGCGCTGATGGCGCATTACTATGCGGAGCGCGCCTCCGCGGGCCTGATCCTCA
CCGAGGCCACGGCCGTCACGCCGCAGGGCGTCGGCTATGCGAACACGCCCGGCATCTGGTCGGACGAGCAGGTCGAGGGC
TGGAAGCTCGTGACGAAGGCGGTCCACGACGCGGGCGGCAAGATCTTCATGCAGCTGTGGCATGTCGGCCGGATCTCGGA
CCCGGTGTTCCTGGACGGCGACCTGCCGGTGGCGCCGAGCGCGATCGCCGCGGGCGGCCACGTGAGCCTGCTGCGCCCGG
AACGTCCGTTCGTCACGCCGCGTGCGCTCGAACTCGACGAAATCCCCGGCATCATCGCCGCCTATCGCAAGGGCGCCGAG
AACGCCAAGCTGGCCGGCTTCGACGGCGTGGAAATCCACGGCGCGAACGGCTACCTGCCCGACCAGTTCCTGCAGGACGG
CACCAACCATCGCACCGACGCCTACGGCGGCTCGATCGAGAACCGCGCGCGCTTCCTGCTCGAGGCCACCGACGCCGCGA
TCGAGGTATGGGGTGCCAAGCGGGTGGGCGTCCACCTGTCGCCGCTCGGCACCGCGCATACGATCAGCGATTCGGACCCG
GTCGCGACCTTCGGTTATGTCGCGCGCGAACTGGGCCGCCGCGGGGTCGCGTTCCTGTTCGTGCGCGAGTCGCTGGGCGA
CGACCGACTCGCGCCGCGCCTGAAGGCCGAGTTCGGCGGCCCGGTGATCGCCAACGACGGCTTCACGCTCGACACCGGCC
TCGCCGTCGTCGCCGCCGGCGAGGCCGACGCGGTGGCCTGGGGCAGGCTGTTCATCGCGAACCCGGACCTGCCCCGCCGT
TTCGAACTCGACACGACGCTGAACACGCCGGTGCCGGCGACGTTCTACGCCGAGGGCGCGACTGGCTACACCGACTACCC
GTCACTCGAAAACATCGCCTGA

Upstream 100 bases:

>100_bases
GATCAGTTGCACACCAAACTTTGACGGCGTTGCCAAGCCGACCGGTGTCCGCACAACGGCTCGTTCGGCGTCTTCGATTT
CGATAGATAAGGAATCCTTC

Downstream 100 bases:

>100_bases
CGCCATACCGCTCCCACCGCGCCCGATGCCTCAGGCCCCCCGGGCGCATGGCCGTACCGTTGCCGGCATGGCCGCGCTTG
CCCTGTTGAATCTCGTGCGG

Product: NADH-flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MPTLFDPLVIGDLTLRNRVVMAPLTRARANQARVPNALMAHYYAERASAGLILTEATAVTPQGVGYANTPGIWSDEQVEG
WKLVTKAVHDAGGKIFMQLWHVGRISDPVFLDGDLPVAPSAIAAGGHVSLLRPERPFVTPRALELDEIPGIIAAYRKGAE
NAKLAGFDGVEIHGANGYLPDQFLQDGTNHRTDAYGGSIENRARFLLEATDAAIEVWGAKRVGVHLSPLGTAHTISDSDP
VATFGYVARELGRRGVAFLFVRESLGDDRLAPRLKAEFGGPVIANDGFTLDTGLAVVAAGEADAVAWGRLFIANPDLPRR
FELDTTLNTPVPATFYAEGATGYTDYPSLENIA

Sequences:

>Translated_353_residues
MPTLFDPLVIGDLTLRNRVVMAPLTRARANQARVPNALMAHYYAERASAGLILTEATAVTPQGVGYANTPGIWSDEQVEG
WKLVTKAVHDAGGKIFMQLWHVGRISDPVFLDGDLPVAPSAIAAGGHVSLLRPERPFVTPRALELDEIPGIIAAYRKGAE
NAKLAGFDGVEIHGANGYLPDQFLQDGTNHRTDAYGGSIENRARFLLEATDAAIEVWGAKRVGVHLSPLGTAHTISDSDP
VATFGYVARELGRRGVAFLFVRESLGDDRLAPRLKAEFGGPVIANDGFTLDTGLAVVAAGEADAVAWGRLFIANPDLPRR
FELDTTLNTPVPATFYAEGATGYTDYPSLENIA
>Mature_352_residues
PTLFDPLVIGDLTLRNRVVMAPLTRARANQARVPNALMAHYYAERASAGLILTEATAVTPQGVGYANTPGIWSDEQVEGW
KLVTKAVHDAGGKIFMQLWHVGRISDPVFLDGDLPVAPSAIAAGGHVSLLRPERPFVTPRALELDEIPGIIAAYRKGAEN
AKLAGFDGVEIHGANGYLPDQFLQDGTNHRTDAYGGSIENRARFLLEATDAAIEVWGAKRVGVHLSPLGTAHTISDSDPV
ATFGYVARELGRRGVAFLFVRESLGDDRLAPRLKAEFGGPVIANDGFTLDTGLAVVAAGEADAVAWGRLFIANPDLPRRF
ELDTTLNTPVPATFYAEGATGYTDYPSLENIA

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=362, Percent_Identity=46.6850828729282, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI1789463, Length=221, Percent_Identity=37.10407239819, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17565138, Length=301, Percent_Identity=28.2392026578073, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17559802, Length=343, Percent_Identity=28.2798833819242, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17559804, Length=212, Percent_Identity=31.6037735849057, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17564188, Length=301, Percent_Identity=27.906976744186, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI72001454, Length=339, Percent_Identity=27.4336283185841, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17540738, Length=358, Percent_Identity=27.0949720670391, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6325086, Length=375, Percent_Identity=39.2, Blast_Score=218, Evalue=9e-58,
Organism=Saccharomyces cerevisiae, GI6321973, Length=378, Percent_Identity=37.5661375661376, Blast_Score=210, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 37796; Mature: 37665

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTLFDPLVIGDLTLRNRVVMAPLTRARANQARVPNALMAHYYAERASAGLILTEATAVT
CCCCCCCEEEECEECCCEEEECCHHHHHCCHHCCCHHHHHHHHHHHCCCCEEEEECEECC
PQGVGYANTPGIWSDEQVEGWKLVTKAVHDAGGKIFMQLWHVGRISDPVFLDGDLPVAPS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEHHHHCCCCCCCEEECCCCCCCCH
AIAAGGHVSLLRPERPFVTPRALELDEIPGIIAAYRKGAENAKLAGFDGVEIHGANGYLP
HHCCCCEEEEECCCCCCCCCCCCCHHHCCHHHHHHHCCCCCCEEECCCCEEEECCCCCCC
DQFLQDGTNHRTDAYGGSIENRARFLLEATDAAIEVWGAKRVGVHLSPLGTAHTISDSDP
HHHHHCCCCCCCCCCCCCHHHHHEEEEEECCCEEEECCCEECCEEECCCCCEEECCCCCC
VATFGYVARELGRRGVAFLFVRESLGDDRLAPRLKAEFGGPVIANDGFTLDTGLAVVAAG
CHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHCCCCEEECCCEEEECCEEEEECC
EADAVAWGRLFIANPDLPRRFELDTTLNTPVPATFYAEGATGYTDYPSLENIA
CCCEEEEEEEEEECCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PTLFDPLVIGDLTLRNRVVMAPLTRARANQARVPNALMAHYYAERASAGLILTEATAVT
CCCCCCEEEECEECCCEEEECCHHHHHCCHHCCCHHHHHHHHHHHCCCCEEEEECEECC
PQGVGYANTPGIWSDEQVEGWKLVTKAVHDAGGKIFMQLWHVGRISDPVFLDGDLPVAPS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEHHHHCCCCCCCEEECCCCCCCCH
AIAAGGHVSLLRPERPFVTPRALELDEIPGIIAAYRKGAENAKLAGFDGVEIHGANGYLP
HHCCCCEEEEECCCCCCCCCCCCCHHHCCHHHHHHHCCCCCCEEECCCCEEEECCCCCCC
DQFLQDGTNHRTDAYGGSIENRARFLLEATDAAIEVWGAKRVGVHLSPLGTAHTISDSDP
HHHHHCCCCCCCCCCCCCHHHHHEEEEEECCCEEEECCCEECCEEECCCCCEEECCCCCC
VATFGYVARELGRRGVAFLFVRESLGDDRLAPRLKAEFGGPVIANDGFTLDTGLAVVAAG
CHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHCCCCEEECCCEEEECCEEEEECC
EADAVAWGRLFIANPDLPRRFELDTTLNTPVPATFYAEGATGYTDYPSLENIA
CCCEEEEEEEEEECCCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]