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The map label for this gene is ydgI [H]

Identifier: 229918319

GI number: 229918319

Start: 2572788

End: 2573426

Strand: Reverse

Name: ydgI [H]

Synonym: EAT1b_2604

Alternate gene names: 229918319

Gene position: 2573426-2572788 (Counterclockwise)

Preceding gene: 229918320

Following gene: 229918318

Centisome position: 85.78

GC content: 48.83

Gene sequence:

>639_bases
ATGGCAACTACATTCGAAAACAATACATTCAAAGACGTTATGCTCGGTCGTAAATCAATCCGTGTCTATGACGAGAACGT
GAAAATCTCACAAGAAGAGATGCTCGAGATGATTCAGGAAGCAACAACTGCCCCATCATCGGTCAACATGCAGCCGTGGC
GCTTCGTCGTCGTCGAAAGCCCAGAAGCGAAAGAGACGCTCAAGCCGCTCATCAAATTCAACACGCGTCAAAACGATTCA
TCTTCAGCGATGCTCCTCATCTTCGGTGACATGGAGTGCTACGAGTACGGCGAACAAATCTACGATCAAGCAGTCGCAGA
AGGCAAAATGCCACAAGAAGTTCGTGACCAGCAACTCGGCGCGATCATCCCTTACTACAAAAACCTCTCGAAACAAGAGA
TGAACGACATCGTGAAAATCGATGCGAGTCTCGCAGCGATGCAATTCATGCTCACGGCACGTGCACACGGCTATGACACG
AACCCAATCGGTGGTTTCGAGAAAGAGCAACTCGCAGAAACGTTCGGTCTCGACAAAGATCGCTACGTACCGGTCATGAT
CTTGTCTCTCGGCAAAGCCGCGGAAGAAGGTTACCAATCAGTACGTCTTGACGCTCAAACAGTCACATCGTTCAAATAA

Upstream 100 bases:

>100_bases
TCGTCTTACAAGCCTTGTCCGCTGAAGAGATTCATCAATTATCCGGACTGCTTCAAAAATTGAACAGTCACGCCTAAACA
GAGAAAGAAGGAATTTACCA

Downstream 100 bases:

>100_bases
TTCAACACACAATCGGAGGAATCAACCATGATCTTAGTCAACGCCACATTCGATATCCAAGCATCACAACGCAAGAACTT
CTTGCGTGACATCCAAGTCT

Product: nitroreductase

Products: NAD(P)(+); @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MATTFENNTFKDVMLGRKSIRVYDENVKISQEEMLEMIQEATTAPSSVNMQPWRFVVVESPEAKETLKPLIKFNTRQNDS
SSAMLLIFGDMECYEYGEQIYDQAVAEGKMPQEVRDQQLGAIIPYYKNLSKQEMNDIVKIDASLAAMQFMLTARAHGYDT
NPIGGFEKEQLAETFGLDKDRYVPVMILSLGKAAEEGYQSVRLDAQTVTSFK

Sequences:

>Translated_212_residues
MATTFENNTFKDVMLGRKSIRVYDENVKISQEEMLEMIQEATTAPSSVNMQPWRFVVVESPEAKETLKPLIKFNTRQNDS
SSAMLLIFGDMECYEYGEQIYDQAVAEGKMPQEVRDQQLGAIIPYYKNLSKQEMNDIVKIDASLAAMQFMLTARAHGYDT
NPIGGFEKEQLAETFGLDKDRYVPVMILSLGKAAEEGYQSVRLDAQTVTSFK
>Mature_211_residues
ATTFENNTFKDVMLGRKSIRVYDENVKISQEEMLEMIQEATTAPSSVNMQPWRFVVVESPEAKETLKPLIKFNTRQNDSS
SAMLLIFGDMECYEYGEQIYDQAVAEGKMPQEVRDQQLGAIIPYYKNLSKQEMNDIVKIDASLAAMQFMLTARAHGYDTN
PIGGFEKEQLAETFGLDKDRYVPVMILSLGKAAEEGYQSVRLDAQTVTSFK

Specific function: Reduction Of A Variety Of Nitroaromatic Compounds Using NADH (And To Lesser Extent Nadph) As Source Of Reducing Equivalents; Two Electrons Are Transferred. Capable Of Reducing Nitrofurazone, Quinones And The Anti-Tumor Agent Cb1954 (5- (Aziridin-1-Yl)-2,4

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 24042; Mature: 23910

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTFENNTFKDVMLGRKSIRVYDENVKISQEEMLEMIQEATTAPSSVNMQPWRFVVVES
CCCCCCCCCHHHHHHCCHHEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEC
PEAKETLKPLIKFNTRQNDSSSAMLLIFGDMECYEYGEQIYDQAVAEGKMPQEVRDQQLG
CCHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHC
AIIPYYKNLSKQEMNDIVKIDASLAAMQFMLTARAHGYDTNPIGGFEKEQLAETFGLDKD
CHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCC
RYVPVMILSLGKAAEEGYQSVRLDAQTVTSFK
CCHHHHEEHHHHHHHHHHHHHCCCHHHHCCCC
>Mature Secondary Structure 
ATTFENNTFKDVMLGRKSIRVYDENVKISQEEMLEMIQEATTAPSSVNMQPWRFVVVES
CCCCCCCCHHHHHHCCHHEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEC
PEAKETLKPLIKFNTRQNDSSSAMLLIFGDMECYEYGEQIYDQAVAEGKMPQEVRDQQLG
CCHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHC
AIIPYYKNLSKQEMNDIVKIDASLAAMQFMLTARAHGYDTNPIGGFEKEQLAETFGLDKD
CHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCC
RYVPVMILSLGKAAEEGYQSVRLDAQTVTSFK
CCHHHHEEHHHHHHHHHHHHHCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H; 6,7-Dihydropteridine [C]

Specific reaction: NAD(P)H + 6,7-Dihydropteridine = NAD(P)(+) + @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]