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The map label for this gene is phoR [H]

Identifier: 229918112

GI number: 229918112

Start: 2342300

End: 2343691

Strand: Reverse

Name: phoR [H]

Synonym: EAT1b_2392

Alternate gene names: 229918112

Gene position: 2343691-2342300 (Counterclockwise)

Preceding gene: 229918113

Following gene: 229918110

Centisome position: 78.13

GC content: 49.78

Gene sequence:

>1392_bases
ATGAAACGATACACGATCAGGAAGCGGATTTGGATTACCATCTGGACAGCCAGCCTCTTCTCAGCGTTCGTGTTTCTCAT
CATGACGTTTTATCTGTACGACAAGTTCTATATCCAAACGCAAGAAGAACTGCTCATCAATCGCGGAGAGAAACTTGTCA
CCATCTATAAGGCCGACGGTTTCTCGGACGCGTTCGCCGATAGTATGACGTACACGAACGAATTGACCGAAACGAAAGTG
TTCCTCTCTTCCGTTCGAGAGGACACACTCGGTTCCGGTAAAACCTTTTTAAGGCAGCAGGACCTCGAACGGTTGCAGGA
CGGATATGCCATCTCGGTGACCCGTAAGCATCCGACCGAGGACGTCGATATTTTGATGACCGCATTCCCTCTCATCCGTG
ACGATGAACTCGACAATGCGCTCATTCTGTACATGGAGCTCAGTAAAATCAGTGAACCGTTCCAACCGATTCGGCTCATG
ATCACGTTTTTACTCGTTCTCATGATTGTGAACTTGCTCCTATTCGGTAAGCAGATCATCGACACGATCATCCAACCGCT
CATTCAGATGAAGCGCGCCTCTCAAGTCTATGCGCAAGGTGATTTCTCCCACCGCATCCCGATCGTCTACGACGATGAGA
TCGGTGAACTCGCAGACACGCTCAACTCGATGGCGGAATCGCTCGGACTTGTTGAAGAGCAGCGTAAAGAGTTTCTGGCT
AACGTCAGTCACGAACTTCGCACGCCACTCTCCTACATCCGCGGTTATACCGAGATGCTTCAAGATGAAACGTTGGACGA
AAAAACGAGGGCGCAATATTACGACATCATCGAGAACGAGACGAACCGACTGCAGCGCCTTGTCACGGACTTACTCGATC
TCGCTCAGCTAGAGCGGGACTCATACCCGATGACGAAAGAACCGGTCGTGTACTCGCAAGTGCTTGAGGACGTTCTCTAT
CGGCTTGAACCGATTGCCCGGGCAAAAGGCGTGACAATCGAATCGGATTTAGACTTCGACTTAATCATTCTCGGTGACCA
TGACCGACTCGAGCAAGTCATCGGCAATCTTCTCGACAACGCGCTCCGATATACGGACGCTGGCAAGACGATTCATGTGA
AGACGTTTGCCGAAACCGAATATGCAATCACAGAAATACGTGACGAAGGTCGAGGCATCCCGAAAGAAGATTTAGACAAA
TTAACGGAACGCTTCTATCGTGTCAATAAATCACGGACTCGAAAAGACGGCGGAACCGGTCTCGGTTTGGCCATTGCGAA
GCACATTATCGAGCGGCATGAAGGATCCCTTCATTTCAGCTCTGAAGTCGGACAAGGAACGACTGTCACTGTCGGTTTGC
CGCTTCTACCTGACGATGAATTTGATATGTAA

Upstream 100 bases:

>100_bases
CGGTCGATACTCACATTAAGACGCTGCGCCTGAAACTCGGCGAGGCGGGCGGTTATATCCAGACCGTTTGGGGGATTGGC
TACAAATTTGAGGCAAACGG

Downstream 100 bases:

>100_bases
AATAGGCAGCCGCGATGGCTGCCTTTTCATATGTCTTTCCCTTTATATTGTTCGGTTGACTCTTGCTCGAGTCGTTCACG
TTCGGCATGTGTCTGCGGAA

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MKRYTIRKRIWITIWTASLFSAFVFLIMTFYLYDKFYIQTQEELLINRGEKLVTIYKADGFSDAFADSMTYTNELTETKV
FLSSVREDTLGSGKTFLRQQDLERLQDGYAISVTRKHPTEDVDILMTAFPLIRDDELDNALILYMELSKISEPFQPIRLM
ITFLLVLMIVNLLLFGKQIIDTIIQPLIQMKRASQVYAQGDFSHRIPIVYDDEIGELADTLNSMAESLGLVEEQRKEFLA
NVSHELRTPLSYIRGYTEMLQDETLDEKTRAQYYDIIENETNRLQRLVTDLLDLAQLERDSYPMTKEPVVYSQVLEDVLY
RLEPIARAKGVTIESDLDFDLIILGDHDRLEQVIGNLLDNALRYTDAGKTIHVKTFAETEYAITEIRDEGRGIPKEDLDK
LTERFYRVNKSRTRKDGGTGLGLAIAKHIIERHEGSLHFSSEVGQGTTVTVGLPLLPDDEFDM

Sequences:

>Translated_463_residues
MKRYTIRKRIWITIWTASLFSAFVFLIMTFYLYDKFYIQTQEELLINRGEKLVTIYKADGFSDAFADSMTYTNELTETKV
FLSSVREDTLGSGKTFLRQQDLERLQDGYAISVTRKHPTEDVDILMTAFPLIRDDELDNALILYMELSKISEPFQPIRLM
ITFLLVLMIVNLLLFGKQIIDTIIQPLIQMKRASQVYAQGDFSHRIPIVYDDEIGELADTLNSMAESLGLVEEQRKEFLA
NVSHELRTPLSYIRGYTEMLQDETLDEKTRAQYYDIIENETNRLQRLVTDLLDLAQLERDSYPMTKEPVVYSQVLEDVLY
RLEPIARAKGVTIESDLDFDLIILGDHDRLEQVIGNLLDNALRYTDAGKTIHVKTFAETEYAITEIRDEGRGIPKEDLDK
LTERFYRVNKSRTRKDGGTGLGLAIAKHIIERHEGSLHFSSEVGQGTTVTVGLPLLPDDEFDM
>Mature_463_residues
MKRYTIRKRIWITIWTASLFSAFVFLIMTFYLYDKFYIQTQEELLINRGEKLVTIYKADGFSDAFADSMTYTNELTETKV
FLSSVREDTLGSGKTFLRQQDLERLQDGYAISVTRKHPTEDVDILMTAFPLIRDDELDNALILYMELSKISEPFQPIRLM
ITFLLVLMIVNLLLFGKQIIDTIIQPLIQMKRASQVYAQGDFSHRIPIVYDDEIGELADTLNSMAESLGLVEEQRKEFLA
NVSHELRTPLSYIRGYTEMLQDETLDEKTRAQYYDIIENETNRLQRLVTDLLDLAQLERDSYPMTKEPVVYSQVLEDVLY
RLEPIARAKGVTIESDLDFDLIILGDHDRLEQVIGNLLDNALRYTDAGKTIHVKTFAETEYAITEIRDEGRGIPKEDLDK
LTERFYRVNKSRTRKDGGTGLGLAIAKHIIERHEGSLHFSSEVGQGTTVTVGLPLLPDDEFDM

Specific function: Member of the two-component regulatory system phoP/phoR involved in the alkaline phosphatase genes regulation. PhoR may function as a membrane-associated protein kinase that phosphorylates phoP in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=273, Percent_Identity=33.6996336996337, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1786600, Length=224, Percent_Identity=37.5, Blast_Score=152, Evalue=6e-38,
Organism=Escherichia coli, GI145693157, Length=243, Percent_Identity=32.5102880658436, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1789149, Length=336, Percent_Identity=26.7857142857143, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1786783, Length=271, Percent_Identity=30.9963099630996, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI48994928, Length=234, Percent_Identity=31.6239316239316, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788713, Length=227, Percent_Identity=28.6343612334802, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87082128, Length=298, Percent_Identity=28.5234899328859, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1790346, Length=279, Percent_Identity=27.5985663082437, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI87081816, Length=338, Percent_Identity=27.5147928994083, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788549, Length=224, Percent_Identity=30.8035714285714, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787894, Length=276, Percent_Identity=28.9855072463768, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1786912, Length=229, Percent_Identity=30.1310043668122, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1790436, Length=232, Percent_Identity=28.8793103448276, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790861, Length=361, Percent_Identity=25.7617728531856, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1790551, Length=316, Percent_Identity=26.2658227848101, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789808, Length=307, Percent_Identity=28.6644951140065, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1788279, Length=228, Percent_Identity=28.5087719298246, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1790300, Length=234, Percent_Identity=29.9145299145299, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787374, Length=225, Percent_Identity=25.3333333333333, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789403, Length=303, Percent_Identity=25.0825082508251, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53353; Mature: 53353

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRYTIRKRIWITIWTASLFSAFVFLIMTFYLYDKFYIQTQEELLINRGEKLVTIYKADG
CCCCEEEHEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEEECCC
FSDAFADSMTYTNELTETKVFLSSVREDTLGSGKTFLRQQDLERLQDGYAISVTRKHPTE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEEECCCCCC
DVDILMTAFPLIRDDELDNALILYMELSKISEPFQPIRLMITFLLVLMIVNLLLFGKQII
HHHHHHHHHCHHCCCCCCCEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DTIIQPLIQMKRASQVYAQGDFSHRIPIVYDDEIGELADTLNSMAESLGLVEEQRKEFLA
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCHHHHHHHHHHH
NVSHELRTPLSYIRGYTEMLQDETLDEKTRAQYYDIIENETNRLQRLVTDLLDLAQLERD
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCC
SYPMTKEPVVYSQVLEDVLYRLEPIARAKGVTIESDLDFDLIILGDHDRLEQVIGNLLDN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEEECCHHHHHHHHHHHHHH
ALRYTDAGKTIHVKTFAETEYAITEIRDEGRGIPKEDLDKLTERFYRVNKSRTRKDGGTG
HHHHCCCCCEEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
LGLAIAKHIIERHEGSLHFSSEVGQGTTVTVGLPLLPDDEFDM
HHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEECCCCCCCCC
>Mature Secondary Structure
MKRYTIRKRIWITIWTASLFSAFVFLIMTFYLYDKFYIQTQEELLINRGEKLVTIYKADG
CCCCEEEHEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEEEECCC
FSDAFADSMTYTNELTETKVFLSSVREDTLGSGKTFLRQQDLERLQDGYAISVTRKHPTE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEEECCCCCC
DVDILMTAFPLIRDDELDNALILYMELSKISEPFQPIRLMITFLLVLMIVNLLLFGKQII
HHHHHHHHHCHHCCCCCCCEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DTIIQPLIQMKRASQVYAQGDFSHRIPIVYDDEIGELADTLNSMAESLGLVEEQRKEFLA
HHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCHHHHHHHHHHH
NVSHELRTPLSYIRGYTEMLQDETLDEKTRAQYYDIIENETNRLQRLVTDLLDLAQLERD
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCC
SYPMTKEPVVYSQVLEDVLYRLEPIARAKGVTIESDLDFDLIILGDHDRLEQVIGNLLDN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEEEECCHHHHHHHHHHHHHH
ALRYTDAGKTIHVKTFAETEYAITEIRDEGRGIPKEDLDKLTERFYRVNKSRTRKDGGTG
HHHHCCCCCEEEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
LGLAIAKHIIERHEGSLHFSSEVGQGTTVTVGLPLLPDDEFDM
HHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3142862; 9387221; 9384377 [H]