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The map label for this gene is menF [H]

Identifier: 229918095

GI number: 229918095

Start: 2327765

End: 2329153

Strand: Reverse

Name: menF [H]

Synonym: EAT1b_2375

Alternate gene names: 229918095

Gene position: 2329153-2327765 (Counterclockwise)

Preceding gene: 229918098

Following gene: 229918094

Centisome position: 77.64

GC content: 48.81

Gene sequence:

>1389_bases
ATGCCAGATACTCAAACACATATAAAACAGCGAATTGAGCAGGCCCATAAACGGGCGCGAGCTTGGCAACGGCCTGTCCT
CGCATCATACACATGGGAAATCTCGGCGTTCGATGCTTTTCAAATCATCCAGAACACCGATACGTCCCACTATTATTTCA
TGACACCAGACCGAACACTCGAAATGCTCGGTTGCGGAGACGCACTCGTCTTGAGCGCTTCTGGACCCGAACGATTTCAG
CGACTTCAATACGATTGGAGTCTTCACCGACGAGATGTCGATGCGACGATCCACGCGTTCGCCGGTTTTTCATTCGACAC
GTTTATGCGACCACAAAAAAGTATGTGGGATGCCTTTGGGGAGGCATCACTCGTCGTACCAAAATTTTTATACCGCCGCT
ATAAAGACCGACACACGTTGACAGTCGCCACCCTCGTCTCTTCGAAGCAGTCGGTCGAACAGTATTTGATTCAATTGGCA
GATCAGTTGGCCCAGTTCAGCTCACTTCGTCCGACGGAAGAAGCGACGCCATCTTATACGAGAGTGAAAGATGGAGTTGA
TCACTTCAAGTCCAGTTTCCAACAGGCGAAACAGTTGATTGAAGAAGAACGCGTTGAAAAAATCGTGATCGCCCGCGAAG
AAATATATCAGCGTTCCGACGATGCATTCCCCTTCGCAAAAAGTTTGAAGCATTTAGCGGATCATCAAGAATCCAGTTAC
ATCTATTTATATCAGCCAAAACGTGACATCGGGTTCTTCGGGGCGACTCCTGAGCGCTTGATTCAAAAAGAGGGCATGCG
ACTGAACACGGCCGCCATCGCAGGGACAATCAAGCGTCCGAAGACCGAGATCGAGGCCGATCTTGCGAAAGATAAACTCT
TAAACGATTCAAAAAATCGTGAAGAGCATCAAATTGTCGTGAAAGACATTAAGCAGGCGTTGACGCCCTATACTGCACCG
ATTCAAACACGAGATACACCGCAAATTTTGGCCAATCGCTCGGTGTTCCATCTACACACCCCAATTCAAGCGACACTCGA
AACGAACGCTTCCTTGCTGTCACTTGCAGAATCGCTACACCCAACGCCGGCACTCGGCGGCTCACCGAAGCGGACATCGG
TCCGGTTGCTTCGTGAAATTGAGCGATTCGATCGTGGATGGTACGGTTCTCCGTTTGGTTGGGTAGACAATGAAGGCGAC
GGCGAATTTGTCGTGGCGATTCGCTCCGCACTCGTTCAACACCAATTCGTCGCATTGTATGCTGGATGTGGGATTGTAAA
AGACTCTGCACTTGAGCAAGAGCTCGAAGAAACAGAAATGAAAATGAGTCCAATCAAACAAGCTTTGCAGTTTGATTTAC
ACAATCAAGGAGGCACCCTAGATGAATAA

Upstream 100 bases:

>100_bases
ATTCTTGATCATTTCCCGAGCATTTCATAGGCAGGTGGATTGCGAACTCATGATACACTAAAGAGTGAGAATTCTATCGG
ATTGAGGAGGCTTTTCTTTT

Downstream 100 bases:

>100_bases
GGTTTTGACCGAATGGGTCGCATCCATGGTAAATCGGCTTGTTACGTCCGGTGTAAAAGACGTGGTCATCAGCCCAGGGT
CCCGCTCGACTCCGCTCGCT

Product: isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MPDTQTHIKQRIEQAHKRARAWQRPVLASYTWEISAFDAFQIIQNTDTSHYYFMTPDRTLEMLGCGDALVLSASGPERFQ
RLQYDWSLHRRDVDATIHAFAGFSFDTFMRPQKSMWDAFGEASLVVPKFLYRRYKDRHTLTVATLVSSKQSVEQYLIQLA
DQLAQFSSLRPTEEATPSYTRVKDGVDHFKSSFQQAKQLIEEERVEKIVIAREEIYQRSDDAFPFAKSLKHLADHQESSY
IYLYQPKRDIGFFGATPERLIQKEGMRLNTAAIAGTIKRPKTEIEADLAKDKLLNDSKNREEHQIVVKDIKQALTPYTAP
IQTRDTPQILANRSVFHLHTPIQATLETNASLLSLAESLHPTPALGGSPKRTSVRLLREIERFDRGWYGSPFGWVDNEGD
GEFVVAIRSALVQHQFVALYAGCGIVKDSALEQELEETEMKMSPIKQALQFDLHNQGGTLDE

Sequences:

>Translated_462_residues
MPDTQTHIKQRIEQAHKRARAWQRPVLASYTWEISAFDAFQIIQNTDTSHYYFMTPDRTLEMLGCGDALVLSASGPERFQ
RLQYDWSLHRRDVDATIHAFAGFSFDTFMRPQKSMWDAFGEASLVVPKFLYRRYKDRHTLTVATLVSSKQSVEQYLIQLA
DQLAQFSSLRPTEEATPSYTRVKDGVDHFKSSFQQAKQLIEEERVEKIVIAREEIYQRSDDAFPFAKSLKHLADHQESSY
IYLYQPKRDIGFFGATPERLIQKEGMRLNTAAIAGTIKRPKTEIEADLAKDKLLNDSKNREEHQIVVKDIKQALTPYTAP
IQTRDTPQILANRSVFHLHTPIQATLETNASLLSLAESLHPTPALGGSPKRTSVRLLREIERFDRGWYGSPFGWVDNEGD
GEFVVAIRSALVQHQFVALYAGCGIVKDSALEQELEETEMKMSPIKQALQFDLHNQGGTLDE
>Mature_461_residues
PDTQTHIKQRIEQAHKRARAWQRPVLASYTWEISAFDAFQIIQNTDTSHYYFMTPDRTLEMLGCGDALVLSASGPERFQR
LQYDWSLHRRDVDATIHAFAGFSFDTFMRPQKSMWDAFGEASLVVPKFLYRRYKDRHTLTVATLVSSKQSVEQYLIQLAD
QLAQFSSLRPTEEATPSYTRVKDGVDHFKSSFQQAKQLIEEERVEKIVIAREEIYQRSDDAFPFAKSLKHLADHQESSYI
YLYQPKRDIGFFGATPERLIQKEGMRLNTAAIAGTIKRPKTEIEADLAKDKLLNDSKNREEHQIVVKDIKQALTPYTAPI
QTRDTPQILANRSVFHLHTPIQATLETNASLLSLAESLHPTPALGGSPKRTSVRLLREIERFDRGWYGSPFGWVDNEGDG
EFVVAIRSALVQHQFVALYAGCGIVKDSALEQELEETEMKMSPIKQALQFDLHNQGGTLDE

Specific function: Siderophore biosynthesis; enterobactin biosynthesis. [C]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=267, Percent_Identity=34.0823970037453, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI87082077, Length=342, Percent_Identity=29.2397660818713, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1787518, Length=240, Percent_Identity=27.9166666666667, Blast_Score=76, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320935, Length=328, Percent_Identity=26.219512195122, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 52786; Mature: 52655

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDTQTHIKQRIEQAHKRARAWQRPVLASYTWEISAFDAFQIIQNTDTSHYYFMTPDRTL
CCCHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEHHHHHHHHHCCCCCEEEEECCCCHH
EMLGCGDALVLSASGPERFQRLQYDWSLHRRDVDATIHAFAGFSFDTFMRPQKSMWDAFG
HHCCCCCEEEEECCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHCCCHHHHHHHHC
EASLVVPKFLYRRYKDRHTLTVATLVSSKQSVEQYLIQLADQLAQFSSLRPTEEATPSYT
CCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
RVKDGVDHFKSSFQQAKQLIEEERVEKIVIAREEIYQRSDDAFPFAKSLKHLADHQESSY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCE
IYLYQPKRDIGFFGATPERLIQKEGMRLNTAAIAGTIKRPKTEIEADLAKDKLLNDSKNR
EEEECCCCCCCCCCCCHHHHHHHCCCEEEHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
EEHQIVVKDIKQALTPYTAPIQTRDTPQILANRSVFHLHTPIQATLETNASLLSLAESLH
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCEEEEECCHHHHHHCCHHHHHHHHHCC
PTPALGGSPKRTSVRLLREIERFDRGWYGSPFGWVDNEGDGEFVVAIRSALVQHQFVALY
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AGCGIVKDSALEQELEETEMKMSPIKQALQFDLHNQGGTLDE
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PDTQTHIKQRIEQAHKRARAWQRPVLASYTWEISAFDAFQIIQNTDTSHYYFMTPDRTL
CCHHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEHHHHHHHHHCCCCCEEEEECCCCHH
EMLGCGDALVLSASGPERFQRLQYDWSLHRRDVDATIHAFAGFSFDTFMRPQKSMWDAFG
HHCCCCCEEEEECCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHCCCHHHHHHHHC
EASLVVPKFLYRRYKDRHTLTVATLVSSKQSVEQYLIQLADQLAQFSSLRPTEEATPSYT
CCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
RVKDGVDHFKSSFQQAKQLIEEERVEKIVIAREEIYQRSDDAFPFAKSLKHLADHQESSY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCE
IYLYQPKRDIGFFGATPERLIQKEGMRLNTAAIAGTIKRPKTEIEADLAKDKLLNDSKNR
EEEECCCCCCCCCCCCHHHHHHHCCCEEEHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCC
EEHQIVVKDIKQALTPYTAPIQTRDTPQILANRSVFHLHTPIQATLETNASLLSLAESLH
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCEEEEECCHHHHHHCCHHHHHHHHHCC
PTPALGGSPKRTSVRLLREIERFDRGWYGSPFGWVDNEGDGEFVVAIRSALVQHQFVALY
CCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AGCGIVKDSALEQELEETEMKMSPIKQALQFDLHNQGGTLDE
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629163; 8566759; 9387221; 9384377; 3131310 [H]