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The map label for this gene is menD [H]

Identifier: 229918094

GI number: 229918094

Start: 2326123

End: 2327772

Strand: Reverse

Name: menD [H]

Synonym: EAT1b_2374

Alternate gene names: 229918094

Gene position: 2327772-2326123 (Counterclockwise)

Preceding gene: 229918095

Following gene: 229918093

Centisome position: 77.6

GC content: 50.36

Gene sequence:

>1650_bases
ATGAATAAGGTTTTGACCGAATGGGTCGCATCCATGGTAAATCGGCTTGTTACGTCCGGTGTAAAAGACGTGGTCATCAG
CCCAGGGTCCCGCTCGACTCCGCTCGCTGTAGCGGCATATCTTCACCCTCAGATTCGTCATCACATCATCGTCGATGAAC
GATCTGCCGGATTTTTCGCTCTTGGACTGACCCGTGCGTCCGAAACGGTTCGCCCGGTCGCATTGATTTGCACAAGTGGA
ACGGCAGCCACAAACTACTATTCGGCTGTGACGGAAGCGTTTATATCGCAGTTGCCACTTGTCGTACTGACGACCGATCG
TCCCCATGAACTGCGTAATGTCGGGGCTCCACAAGCGATCAATCAAGTCCGCCTCTACGGGGAACAAGTGAAGACGAGCG
TTGATTTGCCCGTTCCAGAGGAGGCGACGTTATCGTTTATGCAACAAACGATTACCCGTTTCACGAATCTGTCGATGCAA
GCACCGATGGGACCCGTTCATTTGAATGTGCCGTTCCGAGAACCTCTGCTTCCGGACATCGAGTCATTGAAACAGATATT
GAAGGCTGACCCTGTCTTGCCAGAACGACAGCGTATCCGGCCGACACAGGCGGACAAAGACATTTTCTCAGAACGGACGA
GTCACAAACAACTCGCCTTCGTCGTCGGACCTTACACCCCGACATCTTGGCAGAAATCCATCGTTGAATATGCTTCCGAG
CGACAGATCCCGATTTTCGCCGATCCTCTAAGTGGCATGCGACGTTTCGACGGCGTCTTGACCAACTATGACACGTGGCT
TGCAAGTGAGCATCGGACCGATTGGCTACCCGAGGTGTTCGTCCGTTTCGGGGCTGCACCTGTTTCGAAACGATTCAATC
AATGGATGGCCATGGCGGACCAACTCGTCATCGATGAACCGGGATCTTATCGCGACCCAAGTAGCCGTGCCACGATTTGT
TACGGCGACGCACTCGATTTTCTACCGCTCATCGCATCGGCAAACGAACCTCGGTATCTTGAGCGTTTACGCTTTTTTGA
AGAGATTGCCGAGTCTGCAAAATCGTCACTTCAAGGTGAGGTAGAATTGACGAAACGATTGCTCGATACATCAATCGAGC
TTCTATTCGTCAGTAATAGCATGCCGATTCGAGATGTAGATATATCACTGTCTGCTGGCACGAACATCCACGTTCTCGCC
AATCGAGGCGCAAACGGGATTGATGGTGTGTTATCTACTGCACTCGGAAGCAGTTATGATACGTCTCACGGCGCTCTATT
GATCGGTGATTTGGCGTTCTATCACGATAGCAATGCCTTGCAACTGTTAAAGCATCATCCCGGGAAGTTTTCCGTTGTGA
TTGCAAACAATAATGGTGGAGGAATTTTCTCCTTCCTCCCGCAGGCTGCTGTCGACACACAGTTGTTCGAAGATTTATTC
GGAACACCGCTTGATTTAAATATCCGCGGATTCGCTGACACCTATGGCTTAACGTATCGTCTAATCGAACGACCGGATGA
AATTCAAGAAGCGATTGAAGATGGCATTCATTTAATCGAATTCCCATCGAATCGTGAGGAGAATGTGATGATCCATCGGG
GGTGGACTCAATCGGTCTGCAGTCGTCTCATTACGGAGAACGAATCATGA

Upstream 100 bases:

>100_bases
GACTCTGCACTTGAGCAAGAGCTCGAAGAAACAGAAATGAAAATGAGTCCAATCAAACAAGCTTTGCAGTTTGATTTACA
CAATCAAGGAGGCACCCTAG

Downstream 100 bases:

>100_bases
ACATCGATTTACGTGGCGTACGTTATGAAGTGATTCATCGCGGTGAGGGAAAACCGGTGTTATGTTTACACGGATTCACC
GGAAACGCCCATTGGGTCGA

Product: 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylic acid synthase/2-oxoglutarate decarboxylase

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD [H]

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MNKVLTEWVASMVNRLVTSGVKDVVISPGSRSTPLAVAAYLHPQIRHHIIVDERSAGFFALGLTRASETVRPVALICTSG
TAATNYYSAVTEAFISQLPLVVLTTDRPHELRNVGAPQAINQVRLYGEQVKTSVDLPVPEEATLSFMQQTITRFTNLSMQ
APMGPVHLNVPFREPLLPDIESLKQILKADPVLPERQRIRPTQADKDIFSERTSHKQLAFVVGPYTPTSWQKSIVEYASE
RQIPIFADPLSGMRRFDGVLTNYDTWLASEHRTDWLPEVFVRFGAAPVSKRFNQWMAMADQLVIDEPGSYRDPSSRATIC
YGDALDFLPLIASANEPRYLERLRFFEEIAESAKSSLQGEVELTKRLLDTSIELLFVSNSMPIRDVDISLSAGTNIHVLA
NRGANGIDGVLSTALGSSYDTSHGALLIGDLAFYHDSNALQLLKHHPGKFSVVIANNNGGGIFSFLPQAAVDTQLFEDLF
GTPLDLNIRGFADTYGLTYRLIERPDEIQEAIEDGIHLIEFPSNREENVMIHRGWTQSVCSRLITENES

Sequences:

>Translated_549_residues
MNKVLTEWVASMVNRLVTSGVKDVVISPGSRSTPLAVAAYLHPQIRHHIIVDERSAGFFALGLTRASETVRPVALICTSG
TAATNYYSAVTEAFISQLPLVVLTTDRPHELRNVGAPQAINQVRLYGEQVKTSVDLPVPEEATLSFMQQTITRFTNLSMQ
APMGPVHLNVPFREPLLPDIESLKQILKADPVLPERQRIRPTQADKDIFSERTSHKQLAFVVGPYTPTSWQKSIVEYASE
RQIPIFADPLSGMRRFDGVLTNYDTWLASEHRTDWLPEVFVRFGAAPVSKRFNQWMAMADQLVIDEPGSYRDPSSRATIC
YGDALDFLPLIASANEPRYLERLRFFEEIAESAKSSLQGEVELTKRLLDTSIELLFVSNSMPIRDVDISLSAGTNIHVLA
NRGANGIDGVLSTALGSSYDTSHGALLIGDLAFYHDSNALQLLKHHPGKFSVVIANNNGGGIFSFLPQAAVDTQLFEDLF
GTPLDLNIRGFADTYGLTYRLIERPDEIQEAIEDGIHLIEFPSNREENVMIHRGWTQSVCSRLITENES
>Mature_549_residues
MNKVLTEWVASMVNRLVTSGVKDVVISPGSRSTPLAVAAYLHPQIRHHIIVDERSAGFFALGLTRASETVRPVALICTSG
TAATNYYSAVTEAFISQLPLVVLTTDRPHELRNVGAPQAINQVRLYGEQVKTSVDLPVPEEATLSFMQQTITRFTNLSMQ
APMGPVHLNVPFREPLLPDIESLKQILKADPVLPERQRIRPTQADKDIFSERTSHKQLAFVVGPYTPTSWQKSIVEYASE
RQIPIFADPLSGMRRFDGVLTNYDTWLASEHRTDWLPEVFVRFGAAPVSKRFNQWMAMADQLVIDEPGSYRDPSSRATIC
YGDALDFLPLIASANEPRYLERLRFFEEIAESAKSSLQGEVELTKRLLDTSIELLFVSNSMPIRDVDISLSAGTNIHVLA
NRGANGIDGVLSTALGSSYDTSHGALLIGDLAFYHDSNALQLLKHHPGKFSVVIANNNGGGIFSFLPQAAVDTQLFEDLF
GTPLDLNIRGFADTYGLTYRLIERPDEIQEAIEDGIHLIEFPSNREENVMIHRGWTQSVCSRLITENES

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788599, Length=529, Percent_Identity=32.1361058601134, Blast_Score=211, Evalue=1e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.9 [H]

Molecular weight: Translated: 61009; Mature: 61009

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKVLTEWVASMVNRLVTSGVKDVVISPGSRSTPLAVAAYLHPQIRHHIIVDERSAGFFA
CCHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCEEEHHHHCHHHHEEEEEECCCCCEEE
LGLTRASETVRPVALICTSGTAATNYYSAVTEAFISQLPLVVLTTDRPHELRNVGAPQAI
EEEHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHCCCCHHH
NQVRLYGEQVKTSVDLPVPEEATLSFMQQTITRFTNLSMQAPMGPVHLNVPFREPLLPDI
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCH
ESLKQILKADPVLPERQRIRPTQADKDIFSERTSHKQLAFVVGPYTPTSWQKSIVEYASE
HHHHHHHHCCCCCCCHHHCCCCCCHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHC
RQIPIFADPLSGMRRFDGVLTNYDTWLASEHRTDWLPEVFVRFGAAPVSKRFNQWMAMAD
CCCCEEECCHHHHHHHCCHHHCCHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
QLVIDEPGSYRDPSSRATICYGDALDFLPLIASANEPRYLERLRFFEEIAESAKSSLQGE
HEEEECCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCH
VELTKRLLDTSIELLFVSNSMPIRDVDISLSAGTNIHVLANRGANGIDGVLSTALGSSYD
HHHHHHHHHCCEEEEEEECCCCEEEEEEEEECCCEEEEEECCCCCCHHHHHHHHHCCCCC
TSHGALLIGDLAFYHDSNALQLLKHHPGKFSVVIANNNGGGIFSFLPQAAVDTQLFEDLF
CCCCEEEEEEEEEEECCCHHHHHHCCCCEEEEEEEECCCCCHHHHCCHHHHHHHHHHHHC
GTPLDLNIRGFADTYGLTYRLIERPDEIQEAIEDGIHLIEFPSNREENVMIHRGWTQSVC
CCCEEEEEECCHHHHCCCHHHHCCCHHHHHHHHCCCEEEECCCCCCCCEEEECCCHHHHH
SRLITENES
HHHHCCCCC
>Mature Secondary Structure
MNKVLTEWVASMVNRLVTSGVKDVVISPGSRSTPLAVAAYLHPQIRHHIIVDERSAGFFA
CCHHHHHHHHHHHHHHHHCCCHHEEECCCCCCCCEEEHHHHCHHHHEEEEEECCCCCEEE
LGLTRASETVRPVALICTSGTAATNYYSAVTEAFISQLPLVVLTTDRPHELRNVGAPQAI
EEEHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHCCCCHHH
NQVRLYGEQVKTSVDLPVPEEATLSFMQQTITRFTNLSMQAPMGPVHLNVPFREPLLPDI
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCH
ESLKQILKADPVLPERQRIRPTQADKDIFSERTSHKQLAFVVGPYTPTSWQKSIVEYASE
HHHHHHHHCCCCCCCHHHCCCCCCHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHC
RQIPIFADPLSGMRRFDGVLTNYDTWLASEHRTDWLPEVFVRFGAAPVSKRFNQWMAMAD
CCCCEEECCHHHHHHHCCHHHCCHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
QLVIDEPGSYRDPSSRATICYGDALDFLPLIASANEPRYLERLRFFEEIAESAKSSLQGE
HEEEECCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCH
VELTKRLLDTSIELLFVSNSMPIRDVDISLSAGTNIHVLANRGANGIDGVLSTALGSSYD
HHHHHHHHHCCEEEEEEECCCCEEEEEEEEECCCEEEEEECCCCCCHHHHHHHHHCCCCC
TSHGALLIGDLAFYHDSNALQLLKHHPGKFSVVIANNNGGGIFSFLPQAAVDTQLFEDLF
CCCCEEEEEEEEEEECCCHHHHHHCCCCEEEEEEEECCCCCHHHHCCHHHHHHHHHHHHC
GTPLDLNIRGFADTYGLTYRLIERPDEIQEAIEDGIHLIEFPSNREENVMIHRGWTQSVC
CCCEEEEEECCHHHHCCCHHHHCCCHHHHHHHHCCCEEEECCCCCCCCEEEECCCHHHHH
SRLITENES
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]