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The map label for this gene is ald [H]

Identifier: 229917750

GI number: 229917750

Start: 1980062

End: 1981192

Strand: Reverse

Name: ald [H]

Synonym: EAT1b_2028

Alternate gene names: 229917750

Gene position: 1981192-1980062 (Counterclockwise)

Preceding gene: 229917751

Following gene: 229917747

Centisome position: 66.04

GC content: 51.02

Gene sequence:

>1131_bases
ATGAAAATCGGGGTATTGAAAGAGATTAAAAACAATGAAAATCGGGTGGCCTTAACACCGATGGGTGCATTTATGTTAAC
GGAGCTTGGTCATGATGTATTCGTCGAAACGAATGCCGGTCTCGGTAGCGGCTTCACGAATATGGAATATATTGATGCTG
GTGCGACGATTCTTGAAACGGCGAAAGAAGTTTGGGAAGGCGTCGAACTCGCTCTCAAAGTAAAAGAACCACAACCGTCT
GAGTATCAATACTTCCGTCCTGACTTGACGCTCTTCACTTATCTACACCTTGCGGCGGAGCCGGAATTAACGAAAGCGCT
CGTCGATTCAGGGATTACAGCGATCGCCTACGAGACGGTAGAAGTGGACCGCACGTTACCACTCCTCACACCGATGAGTG
AAGTGGCGGGACGTATGGCTGCGCAAATCGGAGCCCAAATCCTCGAAAAACCACATGGTGGAAAGGGAATCTTAATGTCT
GGTCTCCCAGGTGTGCCTCGTGCGAACGTCACGGTCATCGGAGGCGGTGTCGTCGGAACGAATGCAGCTCGAATCGCAAT
CGGTCTTGGGGCGAGCGTGACGTTGATGGACATTAGCCCGGCTCGTCTTCGTCAAATCGACGACTTGTTCGGAAACACAA
TCAATACGTTGATCTCAAATAAATACAACATTGCGAAACAAGTGGCTGAGAGTGACCTTGTGATTGGTGCCGTGCTCATC
CCAGGAGCAAAAGCACCGAAACTCGTCACGGAAGAAATGGTACAGCGCATGTCGCCTGGTTCGGTCATCGTCGACGTCGC
CATCGACCAAGGTGGAATTTGTGAGACGATCGACCACATCACGACGCATGATGCGCCAACGTATGAGCGCTATGGCGTGC
AACACTACGCTGTCGCTAACATGCCAGGTGCCGTGCCACGCACTTCGACAATGGGTCTGACAAACGCGACGATGCCATAT
ATCATCGAGTGTGCACAAAAAGGCGTATTCCCAGCACTTCTTGAAAACGAGGCGCTTCTCAAAGGCCTAAACGTCATCAA
CGGAACGGTCACGTATGAAGCGGTCGCACGTGATTTGGAATATCCGTTCGTTCCTGCCCATGAGGCGGTCTCGAAACAAA
CACAAGCTTAA

Upstream 100 bases:

>100_bases
GTCTTCGTTTTTAGAATCCATGATAAAGACAGCGTTTTCGAATGGGTCTATACTAGGGGTGTGAACAATTTGTGTCAACG
GAATGAAAGGGGAATGTTCC

Downstream 100 bases:

>100_bases
CCCTTAAGACGATGAGATGCACGTTCTCATCGTCTTTTCTCTATACCAAAAAGAAGGAATCGAACGCCATCGTCCGATTC
CTTCTTTATTATGCGTTATG

Product: alanine dehydrogenase

Products: NA

Alternate protein names: Stage V sporulation protein N [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MKIGVLKEIKNNENRVALTPMGAFMLTELGHDVFVETNAGLGSGFTNMEYIDAGATILETAKEVWEGVELALKVKEPQPS
EYQYFRPDLTLFTYLHLAAEPELTKALVDSGITAIAYETVEVDRTLPLLTPMSEVAGRMAAQIGAQILEKPHGGKGILMS
GLPGVPRANVTVIGGGVVGTNAARIAIGLGASVTLMDISPARLRQIDDLFGNTINTLISNKYNIAKQVAESDLVIGAVLI
PGAKAPKLVTEEMVQRMSPGSVIVDVAIDQGGICETIDHITTHDAPTYERYGVQHYAVANMPGAVPRTSTMGLTNATMPY
IIECAQKGVFPALLENEALLKGLNVINGTVTYEAVARDLEYPFVPAHEAVSKQTQA

Sequences:

>Translated_376_residues
MKIGVLKEIKNNENRVALTPMGAFMLTELGHDVFVETNAGLGSGFTNMEYIDAGATILETAKEVWEGVELALKVKEPQPS
EYQYFRPDLTLFTYLHLAAEPELTKALVDSGITAIAYETVEVDRTLPLLTPMSEVAGRMAAQIGAQILEKPHGGKGILMS
GLPGVPRANVTVIGGGVVGTNAARIAIGLGASVTLMDISPARLRQIDDLFGNTINTLISNKYNIAKQVAESDLVIGAVLI
PGAKAPKLVTEEMVQRMSPGSVIVDVAIDQGGICETIDHITTHDAPTYERYGVQHYAVANMPGAVPRTSTMGLTNATMPY
IIECAQKGVFPALLENEALLKGLNVINGTVTYEAVARDLEYPFVPAHEAVSKQTQA
>Mature_376_residues
MKIGVLKEIKNNENRVALTPMGAFMLTELGHDVFVETNAGLGSGFTNMEYIDAGATILETAKEVWEGVELALKVKEPQPS
EYQYFRPDLTLFTYLHLAAEPELTKALVDSGITAIAYETVEVDRTLPLLTPMSEVAGRMAAQIGAQILEKPHGGKGILMS
GLPGVPRANVTVIGGGVVGTNAARIAIGLGASVTLMDISPARLRQIDDLFGNTINTLISNKYNIAKQVAESDLVIGAVLI
PGAKAPKLVTEEMVQRMSPGSVIVDVAIDQGGICETIDHITTHDAPTYERYGVQHYAVANMPGAVPRTSTMGLTNATMPY
IIECAQKGVFPALLENEALLKGLNVINGTVTYEAVARDLEYPFVPAHEAVSKQTQA

Specific function: This enzyme is a key factor in the assimilation of L- alanine as an energy source through the tricarboxylic acid cycle during sporulation [H]

COG id: COG0686

COG function: function code E; Alanine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=331, Percent_Identity=31.1178247734139, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI122939153, Length=331, Percent_Identity=31.1178247734139, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI1787887, Length=379, Percent_Identity=31.6622691292876, Blast_Score=143, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17550456, Length=375, Percent_Identity=29.6, Blast_Score=143, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR008141 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.4.1.1 [H]

Molecular weight: Translated: 40172; Mature: 40172

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGVLKEIKNNENRVALTPMGAFMLTELGHDVFVETNAGLGSGFTNMEYIDAGATILET
CCCCCHHHHCCCCCEEEEECCHHHHHHHCCCEEEEEECCCCCCCCCCCEEECCCHHHHHH
AKEVWEGVELALKVKEPQPSEYQYFRPDLTLFTYLHLAAEPELTKALVDSGITAIAYETV
HHHHHCCEEEEEEECCCCCCCCEEECCCHHHHHHHHHHCCCHHHHHHHHCCCEEEEEEEE
EVDRTLPLLTPMSEVAGRMAAQIGAQILEKPHGGKGILMSGLPGVPRANVTVIGGGVVGT
ECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCEEEEECCEECC
NAARIAIGLGASVTLMDISPARLRQIDDLFGNTINTLISNKYNIAKQVAESDLVIGAVLI
CCEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEEEEE
PGAKAPKLVTEEMVQRMSPGSVIVDVAIDQGGICETIDHITTHDAPTYERYGVQHYAVAN
CCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCHHHHCCCEEEEEC
MPGAVPRTSTMGLTNATMPYIIECAQKGVFPALLENEALLKGLNVINGTVTYEAVARDLE
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCEEEHHHHHHHCC
YPFVPAHEAVSKQTQA
CCCCCCHHHHHHHCCC
>Mature Secondary Structure
MKIGVLKEIKNNENRVALTPMGAFMLTELGHDVFVETNAGLGSGFTNMEYIDAGATILET
CCCCCHHHHCCCCCEEEEECCHHHHHHHCCCEEEEEECCCCCCCCCCCEEECCCHHHHHH
AKEVWEGVELALKVKEPQPSEYQYFRPDLTLFTYLHLAAEPELTKALVDSGITAIAYETV
HHHHHCCEEEEEEECCCCCCCCEEECCCHHHHHHHHHHCCCHHHHHHHHCCCEEEEEEEE
EVDRTLPLLTPMSEVAGRMAAQIGAQILEKPHGGKGILMSGLPGVPRANVTVIGGGVVGT
ECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCEEEEECCEECC
NAARIAIGLGASVTLMDISPARLRQIDDLFGNTINTLISNKYNIAKQVAESDLVIGAVLI
CCEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEEEEE
PGAKAPKLVTEEMVQRMSPGSVIVDVAIDQGGICETIDHITTHDAPTYERYGVQHYAVAN
CCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHCCCCCCHHHHCCCEEEEEC
MPGAVPRTSTMGLTNATMPYIIECAQKGVFPALLENEALLKGLNVINGTVTYEAVARDLE
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCEEEHHHHHHHCC
YPFVPAHEAVSKQTQA
CCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8226620; 9384377 [H]