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The map label for this gene is acoB [H]

Identifier: 227827161

GI number: 227827161

Start: 793536

End: 794510

Strand: Direct

Name: acoB [H]

Synonym: M1425_0841

Alternate gene names: 227827161

Gene position: 793536-794510 (Clockwise)

Preceding gene: 227827160

Following gene: 227827162

Centisome position: 30.42

GC content: 39.9

Gene sequence:

>975_bases
ATGAGGCAGATAACGTTTACCGAAGCAATAACTGAAGCTTTAAGGCAAGAAATGGAAAGAGATCCCTCAGTCATATTAAT
AGGAGAAGATATAGGTGTTTATGGGGGCGCGTTTGGAGTAACCAAGGGTTTGGTAGAGAAATTTGGCTCAGATAGGGTTA
TTGATACTCCAATATCTGAGGCTGGATTTATAGGTGCTGCAGTAGGTGCTGCGCTAGCTGGCTTAAGACCAGTAGTGGAG
CTAATGTTTGTAGATTTCTTTGGAGTAGCAATGGATCAGATCTATAATCAAATGGCTAAATTAAGGTATATGTCTGGAGG
GCAATTGAAAGTTCCCCTTACTTTAAGGGCGCCGATAGGGGCGGGAATAAGTGCTGCAGCTCAACATTCACAAACTCTTT
ACTCAATTTTCGCACACGTACCCGGTTTAAAAGTAGTAGTACCCTCTACTCCTCATGACGCTAAAGGTTTATTGATCTCA
TCAATACATGACGATAATCCAGTAGTATTCTTAGAACATAAGGTGTTATATGGGATAAAAGGTGAGGTACCGGAGGAAGA
ATACACTATACCTCTAGGCAAGGCAGATATAAAAAGGGAAGGAAGTGACATAACAATAATTGGAATAGCCAGAACAGTAT
GGCATAGCCTAGAAGCTGCGGAGCAACTATCTAAGGAAGGTATAAGTGTAGAGGTTATTGATGTGAGATCAATAGTTCCT
TTTGATAAGGAAACTGTTATTAAATCAGTTAAGAAGACAGGGAGAGTTGTAATTGTGGATGAGGATTATGACAGATGTGG
TTTCGCCTCATGGGTTTCTTCCATAATTGCAGATGAAGCTTTTGAATACTTGGATGCGCCAATAAAGAGAATAACCACTC
CTAACGTCCCTATACCTTTCAGCCCTCCCTTAGAGCAATATATATTACCAGATTCCAAAAAGATTATTAATACAGTTAAA
TCCATATTGGGGTAA

Upstream 100 bases:

>100_bases
AGAGCAGGCATTGAAATTCGCTGAGGAAAGTCCATATCCAGAAGTGGAAGAGGCTCTAACTGACGTTTTTACAGATAATT
CGTACTAGGGTGATATGTAA

Downstream 100 bases:

>100_bases
ATTTTAGTTGACATCTATAGGAATTATTATTAACCCTGAATCTGGGAGAGACATTAGAAGACTAGTTGCAAAAGCTACCT
TTGTCTCTAATTACGCTAAA

Product: transketolase

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-beta; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit beta [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MRQITFTEAITEALRQEMERDPSVILIGEDIGVYGGAFGVTKGLVEKFGSDRVIDTPISEAGFIGAAVGAALAGLRPVVE
LMFVDFFGVAMDQIYNQMAKLRYMSGGQLKVPLTLRAPIGAGISAAAQHSQTLYSIFAHVPGLKVVVPSTPHDAKGLLIS
SIHDDNPVVFLEHKVLYGIKGEVPEEEYTIPLGKADIKREGSDITIIGIARTVWHSLEAAEQLSKEGISVEVIDVRSIVP
FDKETVIKSVKKTGRVVIVDEDYDRCGFASWVSSIIADEAFEYLDAPIKRITTPNVPIPFSPPLEQYILPDSKKIINTVK
SILG

Sequences:

>Translated_324_residues
MRQITFTEAITEALRQEMERDPSVILIGEDIGVYGGAFGVTKGLVEKFGSDRVIDTPISEAGFIGAAVGAALAGLRPVVE
LMFVDFFGVAMDQIYNQMAKLRYMSGGQLKVPLTLRAPIGAGISAAAQHSQTLYSIFAHVPGLKVVVPSTPHDAKGLLIS
SIHDDNPVVFLEHKVLYGIKGEVPEEEYTIPLGKADIKREGSDITIIGIARTVWHSLEAAEQLSKEGISVEVIDVRSIVP
FDKETVIKSVKKTGRVVIVDEDYDRCGFASWVSSIIADEAFEYLDAPIKRITTPNVPIPFSPPLEQYILPDSKKIINTVK
SILG
>Mature_324_residues
MRQITFTEAITEALRQEMERDPSVILIGEDIGVYGGAFGVTKGLVEKFGSDRVIDTPISEAGFIGAAVGAALAGLRPVVE
LMFVDFFGVAMDQIYNQMAKLRYMSGGQLKVPLTLRAPIGAGISAAAQHSQTLYSIFAHVPGLKVVVPSTPHDAKGLLIS
SIHDDNPVVFLEHKVLYGIKGEVPEEEYTIPLGKADIKREGSDITIIGIARTVWHSLEAAEQLSKEGISVEVIDVRSIVP
FDKETVIKSVKKTGRVVIVDEDYDRCGFASWVSSIIADEAFEYLDAPIKRITTPNVPIPFSPPLEQYILPDSKKIINTVK
SILG

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde [H]

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI156564403, Length=326, Percent_Identity=46.6257668711656, Blast_Score=298, Evalue=6e-81,
Organism=Homo sapiens, GI291084858, Length=327, Percent_Identity=44.3425076452599, Blast_Score=275, Evalue=5e-74,
Organism=Homo sapiens, GI4557353, Length=327, Percent_Identity=40.3669724770642, Blast_Score=253, Evalue=1e-67,
Organism=Homo sapiens, GI34101272, Length=327, Percent_Identity=40.3669724770642, Blast_Score=253, Evalue=1e-67,
Organism=Homo sapiens, GI133778974, Length=243, Percent_Identity=30.4526748971193, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI225637461, Length=240, Percent_Identity=26.25, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI225637459, Length=240, Percent_Identity=26.25, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI225637463, Length=240, Percent_Identity=26.25, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI205277463, Length=244, Percent_Identity=26.6393442622951, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI4507521, Length=244, Percent_Identity=26.6393442622951, Blast_Score=67, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17538422, Length=326, Percent_Identity=45.398773006135, Blast_Score=294, Evalue=4e-80,
Organism=Caenorhabditis elegans, GI17506935, Length=322, Percent_Identity=40.6832298136646, Blast_Score=221, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI17539652, Length=262, Percent_Identity=29.0076335877863, Blast_Score=71, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6319698, Length=328, Percent_Identity=47.5609756097561, Blast_Score=307, Evalue=1e-84,
Organism=Drosophila melanogaster, GI21358145, Length=328, Percent_Identity=43.2926829268293, Blast_Score=281, Evalue=4e-76,
Organism=Drosophila melanogaster, GI24650940, Length=328, Percent_Identity=43.2926829268293, Blast_Score=281, Evalue=4e-76,
Organism=Drosophila melanogaster, GI160714832, Length=326, Percent_Identity=41.1042944785276, Blast_Score=236, Evalue=1e-62,
Organism=Drosophila melanogaster, GI160714828, Length=326, Percent_Identity=41.1042944785276, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24650943, Length=92, Percent_Identity=50, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24650945, Length=92, Percent_Identity=50, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24645119, Length=288, Percent_Identity=26.7361111111111, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI45551847, Length=288, Percent_Identity=26.7361111111111, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI45550715, Length=288, Percent_Identity=26.7361111111111, Blast_Score=79, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: 1.2.4.1

Molecular weight: Translated: 35220; Mature: 35220

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQITFTEAITEALRQEMERDPSVILIGEDIGVYGGAFGVTKGLVEKFGSDRVIDTPISE
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEECCCCC
AGFIGAAVGAALAGLRPVVELMFVDFFGVAMDQIYNQMAKLRYMSGGQLKVPLTLRAPIG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCC
AGISAAAQHSQTLYSIFAHVPGLKVVVPSTPHDAKGLLISSIHDDNPVVFLEHKVLYGIK
CCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECCCCCEEEEECEEEECCC
GEVPEEEYTIPLGKADIKREGSDITIIGIARTVWHSLEAAEQLSKEGISVEVIDVRSIVP
CCCCCCCEEECCCCHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCEEEEEEEHHCCC
FDKETVIKSVKKTGRVVIVDEDYDRCGFASWVSSIIADEAFEYLDAPIKRITTPNVPIPF
CCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
SPPLEQYILPDSKKIINTVKSILG
CCCHHHHCCCCHHHHHHHHHHHHC
>Mature Secondary Structure
MRQITFTEAITEALRQEMERDPSVILIGEDIGVYGGAFGVTKGLVEKFGSDRVIDTPISE
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEECCCCC
AGFIGAAVGAALAGLRPVVELMFVDFFGVAMDQIYNQMAKLRYMSGGQLKVPLTLRAPIG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCC
AGISAAAQHSQTLYSIFAHVPGLKVVVPSTPHDAKGLLISSIHDDNPVVFLEHKVLYGIK
CCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECCCCCEEEEECEEEECCC
GEVPEEEYTIPLGKADIKREGSDITIIGIARTVWHSLEAAEQLSKEGISVEVIDVRSIVP
CCCCCCCEEECCCCHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHCCCEEEEEEEHHCCC
FDKETVIKSVKKTGRVVIVDEDYDRCGFASWVSSIIADEAFEYLDAPIKRITTPNVPIPF
CCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
SPPLEQYILPDSKKIINTVKSILG
CCCHHHHCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10368162; 8969503; 9384377 [H]