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The map label for this gene is acoA [H]

Identifier: 227827160

GI number: 227827160

Start: 792525

End: 793523

Strand: Direct

Name: acoA [H]

Synonym: M1425_0840

Alternate gene names: 227827160

Gene position: 792525-793523 (Clockwise)

Preceding gene: 227827159

Following gene: 227827161

Centisome position: 30.38

GC content: 38.64

Gene sequence:

>999_bases
ATGGTGCAAGATATCCCTAAAAGTAAATTATTAGACATGTATAAAAAGATGCTATTAATTAGATATCATGAGCTTACTGC
TAAAGAGCTCTTTGCTATTGGTAAGATTCCGGGATTTGTTCATCTCTATGTAGGTGAAGAGGCTGTAGCTGTAGGAGTTA
TGAGCACGCTAAGAGATGATGATTATATAACTAGCACACATAGAGGGCATGGGCATTGTATAGCCAAGGGTTTAGACGTA
AAGAGAATGTTAGCAGAGATAATGGGTAAGAAAACTGGAGTGTGTAAAGGAAAAGGTGGATCGATGCATATTTTTGATTA
TAGTAAAGGTATGTTAGGTGCAAATGGTATAGTTGGTGGAGGAGCTCCTCATGCAGTAGGTGCAGCATTAGCGTTTAAAC
TTAAGGGTTTAGATCGTGTAGCTGCAGCGTTTATTGGAGATGGAGCTATGAATCAAGGTGTAGTTTTGGAATCTTTAAAC
CTCTCTGCCATATGGAAACTTCCAGTAGTCTTTATAGTAGAAGATAACATGTATGCGATGTCGACTAGAAGTTTAGCCCC
AGCTAAATTACAGCCTAGTCATTCTGCTGCTAAAAGTTACGTCGAGAGAGCTTTAGGTTTTGGAATACCCGCTGTAGAAG
TGGATGGTATGGATGTACTAGCAGTTTATGAGGTGGCAAAAGAAGCTGTTGATAAGGCAAGAAAAGGAGGAGGTCCATCC
TTATTACACTGTAAGACTTACAGGTTTTTTGGACACTTTGAGGGAGATTCTTTAGTTTATAGGGATAAAGAGGAGGAAGA
GATGTGGAGAAAAAGAGATCCAATTACTCTATTTAGAGATAAACTTGTTTCAAATAATATCGTAAATCAAGAGGAACTGG
ATAAGATAGATAGAGAAGCTAAAACCGAAATAGAGCAGGCATTGAAATTCGCTGAGGAAAGTCCATATCCAGAAGTGGAA
GAGGCTCTAACTGACGTTTTTACAGATAATTCGTACTAG

Upstream 100 bases:

>100_bases
AAACTGATATAAAATTGCTGACAGTGACGTTGTCTTCTCCTCTCATAGCAATTTTGTTAACATTTAAATACTTCAATTTT
AATATATTCTTATTGAGATA

Downstream 100 bases:

>100_bases
GGTGATATGTAAATGAGGCAGATAACGTTTACCGAAGCAATAACTGAAGCTTTAAGGCAAGAAATGGAAAGAGATCCCTC
AGTCATATTAATAGGAGAAG

Product: dehydrogenase E1 component

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit alpha [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MVQDIPKSKLLDMYKKMLLIRYHELTAKELFAIGKIPGFVHLYVGEEAVAVGVMSTLRDDDYITSTHRGHGHCIAKGLDV
KRMLAEIMGKKTGVCKGKGGSMHIFDYSKGMLGANGIVGGGAPHAVGAALAFKLKGLDRVAAAFIGDGAMNQGVVLESLN
LSAIWKLPVVFIVEDNMYAMSTRSLAPAKLQPSHSAAKSYVERALGFGIPAVEVDGMDVLAVYEVAKEAVDKARKGGGPS
LLHCKTYRFFGHFEGDSLVYRDKEEEEMWRKRDPITLFRDKLVSNNIVNQEELDKIDREAKTEIEQALKFAEESPYPEVE
EALTDVFTDNSY

Sequences:

>Translated_332_residues
MVQDIPKSKLLDMYKKMLLIRYHELTAKELFAIGKIPGFVHLYVGEEAVAVGVMSTLRDDDYITSTHRGHGHCIAKGLDV
KRMLAEIMGKKTGVCKGKGGSMHIFDYSKGMLGANGIVGGGAPHAVGAALAFKLKGLDRVAAAFIGDGAMNQGVVLESLN
LSAIWKLPVVFIVEDNMYAMSTRSLAPAKLQPSHSAAKSYVERALGFGIPAVEVDGMDVLAVYEVAKEAVDKARKGGGPS
LLHCKTYRFFGHFEGDSLVYRDKEEEEMWRKRDPITLFRDKLVSNNIVNQEELDKIDREAKTEIEQALKFAEESPYPEVE
EALTDVFTDNSY
>Mature_332_residues
MVQDIPKSKLLDMYKKMLLIRYHELTAKELFAIGKIPGFVHLYVGEEAVAVGVMSTLRDDDYITSTHRGHGHCIAKGLDV
KRMLAEIMGKKTGVCKGKGGSMHIFDYSKGMLGANGIVGGGAPHAVGAALAFKLKGLDRVAAAFIGDGAMNQGVVLESLN
LSAIWKLPVVFIVEDNMYAMSTRSLAPAKLQPSHSAAKSYVERALGFGIPAVEVDGMDVLAVYEVAKEAVDKARKGGGPS
LLHCKTYRFFGHFEGDSLVYRDKEEEEMWRKRDPITLFRDKLVSNNIVNQEELDKIDREAKTEIEQALKFAEESPYPEVE
EALTDVFTDNSY

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=329, Percent_Identity=41.3373860182371, Blast_Score=256, Evalue=3e-68,
Organism=Homo sapiens, GI291084742, Length=322, Percent_Identity=41.9254658385093, Blast_Score=224, Evalue=8e-59,
Organism=Homo sapiens, GI4505685, Length=322, Percent_Identity=41.9254658385093, Blast_Score=224, Evalue=9e-59,
Organism=Homo sapiens, GI291084744, Length=329, Percent_Identity=41.0334346504559, Blast_Score=217, Evalue=1e-56,
Organism=Homo sapiens, GI291084757, Length=322, Percent_Identity=37.5776397515528, Blast_Score=185, Evalue=6e-47,
Organism=Homo sapiens, GI258645172, Length=327, Percent_Identity=28.4403669724771, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI11386135, Length=327, Percent_Identity=28.7461773700306, Blast_Score=132, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI32564172, Length=319, Percent_Identity=38.5579937304075, Blast_Score=223, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI17536047, Length=319, Percent_Identity=38.5579937304075, Blast_Score=222, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI86563357, Length=320, Percent_Identity=28.125, Blast_Score=118, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI86563355, Length=320, Percent_Identity=28.125, Blast_Score=118, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6321026, Length=328, Percent_Identity=39.6341463414634, Blast_Score=231, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24639740, Length=328, Percent_Identity=38.4146341463415, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24639744, Length=328, Percent_Identity=38.4146341463415, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI28571106, Length=328, Percent_Identity=38.4146341463415, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24639746, Length=316, Percent_Identity=38.9240506329114, Blast_Score=209, Evalue=3e-54,
Organism=Drosophila melanogaster, GI24639748, Length=329, Percent_Identity=36.7781155015198, Blast_Score=207, Evalue=6e-54,
Organism=Drosophila melanogaster, GI21355903, Length=323, Percent_Identity=24.7678018575851, Blast_Score=102, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 36550; Mature: 36550

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQDIPKSKLLDMYKKMLLIRYHELTAKELFAIGKIPGFVHLYVGEEAVAVGVMSTLRDD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCC
DYITSTHRGHGHCIAKGLDVKRMLAEIMGKKTGVCKGKGGSMHIFDYSKGMLGANGIVGG
CCEECCCCCCCEEEECCCCHHHHHHHHHCCCCCCEECCCCEEEEEECCCCCCCCCCCCCC
GAPHAVGAALAFKLKGLDRVAAAFIGDGAMNQGVVLESLNLSAIWKLPVVFIVEDNMYAM
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCEEEEEECCEEEE
STRSLAPAKLQPSHSAAKSYVERALGFGIPAVEVDGMDVLAVYEVAKEAVDKARKGGGPS
ECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCC
LLHCKTYRFFGHFEGDSLVYRDKEEEEMWRKRDPITLFRDKLVSNNIVNQEELDKIDREA
EEEEEEEEEEEEECCCEEEECCCCHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
KTEIEQALKFAEESPYPEVEEALTDVFTDNSY
HHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVQDIPKSKLLDMYKKMLLIRYHELTAKELFAIGKIPGFVHLYVGEEAVAVGVMSTLRDD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCC
DYITSTHRGHGHCIAKGLDVKRMLAEIMGKKTGVCKGKGGSMHIFDYSKGMLGANGIVGG
CCEECCCCCCCEEEECCCCHHHHHHHHHCCCCCCEECCCCEEEEEECCCCCCCCCCCCCC
GAPHAVGAALAFKLKGLDRVAAAFIGDGAMNQGVVLESLNLSAIWKLPVVFIVEDNMYAM
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEEEECCEEEEEECCEEEE
STRSLAPAKLQPSHSAAKSYVERALGFGIPAVEVDGMDVLAVYEVAKEAVDKARKGGGPS
ECCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCC
LLHCKTYRFFGHFEGDSLVYRDKEEEEMWRKRDPITLFRDKLVSNNIVNQEELDKIDREA
EEEEEEEEEEEEECCCEEEECCCCHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
KTEIEQALKFAEESPYPEVEEALTDVFTDNSY
HHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 10368162; 9384377 [H]