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The map label for this gene is ntaB [H]

Identifier: 227827026

GI number: 227827026

Start: 638128

End: 638595

Strand: Direct

Name: ntaB [H]

Synonym: M1425_0684

Alternate gene names: 227827026

Gene position: 638128-638595 (Clockwise)

Preceding gene: 227827025

Following gene: 227827027

Centisome position: 24.46

GC content: 33.76

Gene sequence:

>468_bases
ATGAGCGAGACGATAAGGAATATAATGAGATTATTTCCGCTAGGAATAGTAGTAGTTACAACTAAATGGAGTGATAATTT
AGTAGGAATGACGGTCAACACATTTAATTCGCTTTCTTTAAATCCACCATTAGTTATGTTTACCGCAGATAAAACTAAGG
GTAATGATGTACCTTTTATCGAGAGTAATGGTTTTATAGTAAATTTTGTCGATGACGAAAAAATCTTCAATACATTTGCC
TTTAAGCCCGTAAAGGAGAGATTCAAGGAGGTAAAGTTCTTCGAAGGTTTAGATGGAATACCCGTATTGGCTGACAGTTA
CGCTTACATGGAAGTTAAGAAGTATAAGACTATTGATATTGGTGATCACACCATTGTAGTTGGTGAGGTAATTAATGGAA
AGGTTGTTAGAGATAATTTCTCACCCTTAGTCTATTACAATAGAAATTATTGGAAGTTATGTAGATAA

Upstream 100 bases:

>100_bases
GGACATAGGTCTGCTAATAAGAGATTGGATGAGGTTTCACCCTAAGACATCAACTCAGTTTAATGATAAACTTAAATAAT
TTTTTTAAGTATATACTCTC

Downstream 100 bases:

>100_bases
CCTTAAATCAGTTTAATAGTGAATTAATTTATCTTTTTGCACTTGATAGTGTTTGATGATCAACATTTTACGTACATCTC
ATGTGTGTATAAGAGTTAGC

Product: flavin reductase domain protein FMN-binding

Products: NAD(+); reduced flavin [C]

Alternate protein names: NTA monooxygenase component B; NTA-MO B [H]

Number of amino acids: Translated: 155; Mature: 154

Protein sequence:

>155_residues
MSETIRNIMRLFPLGIVVVTTKWSDNLVGMTVNTFNSLSLNPPLVMFTADKTKGNDVPFIESNGFIVNFVDDEKIFNTFA
FKPVKERFKEVKFFEGLDGIPVLADSYAYMEVKKYKTIDIGDHTIVVGEVINGKVVRDNFSPLVYYNRNYWKLCR

Sequences:

>Translated_155_residues
MSETIRNIMRLFPLGIVVVTTKWSDNLVGMTVNTFNSLSLNPPLVMFTADKTKGNDVPFIESNGFIVNFVDDEKIFNTFA
FKPVKERFKEVKFFEGLDGIPVLADSYAYMEVKKYKTIDIGDHTIVVGEVINGKVVRDNFSPLVYYNRNYWKLCR
>Mature_154_residues
SETIRNIMRLFPLGIVVVTTKWSDNLVGMTVNTFNSLSLNPPLVMFTADKTKGNDVPFIESNGFIVNFVDDEKIFNTFAF
KPVKERFKEVKFFEGLDGIPVLADSYAYMEVKKYKTIDIGDHTIVVGEVINGKVVRDNFSPLVYYNRNYWKLCR

Specific function: Hydroxylation of nitrilotriacetate [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 17808; Mature: 17677

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETIRNIMRLFPLGIVVVTTKWSDNLVGMTVNTFNSLSLNPPLVMFTADKTKGNDVPFI
CCHHHHHHHHHCCCEEEEEEEECCCCEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCEE
ESNGFIVNFVDDEKIFNTFAFKPVKERFKEVKFFEGLDGIPVLADSYAYMEVKKYKTIDI
ECCCEEEEEECCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEECCEEEEEEEEEEEEEC
GDHTIVVGEVINGKVVRDNFSPLVYYNRNYWKLCR
CCCEEEEEEECCCEEEECCCCEEEEEECCEEEECC
>Mature Secondary Structure 
SETIRNIMRLFPLGIVVVTTKWSDNLVGMTVNTFNSLSLNPPLVMFTADKTKGNDVPFI
CHHHHHHHHHCCCEEEEEEEECCCCEEEEEEECCCCCCCCCCEEEEEECCCCCCCCCEE
ESNGFIVNFVDDEKIFNTFAFKPVKERFKEVKFFEGLDGIPVLADSYAYMEVKKYKTIDI
ECCCEEEEEECCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEECCEEEEEEEEEEEEEC
GDHTIVVGEVINGKVVRDNFSPLVYYNRNYWKLCR
CCCEEEEEEECCCEEEECCCCEEEEEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8892809; 9023192; 1735711 [H]