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The map label for this gene is bphC [H]

Identifier: 227827027

GI number: 227827027

Start: 638651

End: 639598

Strand: Direct

Name: bphC [H]

Synonym: M1425_0685

Alternate gene names: 227827027

Gene position: 638651-639598 (Clockwise)

Preceding gene: 227827026

Following gene: 227827028

Centisome position: 24.48

GC content: 39.66

Gene sequence:

>948_bases
ATGATCAACATTTTACGTACATCTCATGTGTGTATAAGAGTTAGCGATTTGGATAAGGCGAGATACTTCTACTCAGAACT
CTTAGGATTTGTGGAGACAGAAAAGGATGGCGATTACATTTATTTGAGAGGGATTGAGGAAGGTCAGCATCATAGCTTAA
TACTAAAAAAGGCCAACTCGGCTGGTTTATCGTATATAGGCTTTAGGGTAAGTAAAGGAGAAGAATTAGATAAGGCCAAA
GAAGAATTGGAAAACCTAGGTCATAAGGTTAGGAAATTTAGGGAAAAGGGTATTAATGAAGCTCTATTATTTGAATCCCC
CGAAGGAATCCCAATACTTCTATACTATGAAATGGAGTATGTAGGCGATTTAAGATTAGCATTTCATAGGCATAGGGGAG
TGAGTCCAGTCAGGTTAGCCCACGTAAATTATATGGTTAAGGACTTGGAGAGAGAATTGGAGTTCTTTAAGAGATTCATG
GGTTATTATGAGACTGAGAGGTTCTTGGATAAGGATGGGAATTTGACAGTAGTGTGGTTAACTAGAAGAGGGGATTCACA
CGAAATAGCAATAGCGAAGTCAAGTAAAAACGTTCCGGGATTTCACCATGAGACGTATTACGTTCATGATGTAAAAGATG
TCATTAAAGCCCCAGATATTCTAGCCTCAGTGGGATTGTGGGATAGCATAGAAAGGGGTCCCGGTAGACATGGTGTAACT
GAGGGTTACTATATTTATTTGAGAGATTTTGATAAAAACAGAATTGAATTCTTCACCAACGATTACGTGGTTCTGGATCC
GGATAAGTGGAAACCAATAGTTTGGACCTATGATCAATTAAGGTATAGAAGTGATTTTTGGGGAAGGCCGATTCCTCAAT
CTTGGTTAACTGAATGGGTTCCCGTGGAGGATATTTCCACGGGTCAATTGAAGGGGTGGTCTGGATGA

Upstream 100 bases:

>100_bases
CCCTTAGTCTATTACAATAGAAATTATTGGAAGTTATGTAGATAACCTTAAATCAGTTTAATAGTGAATTAATTTATCTT
TTTGCACTTGATAGTGTTTG

Downstream 100 bases:

>100_bases
TAAGAAGGGGAACAGATTACATAAAAAGTATAAAGGAAAACCCACCAGTAGTTTACTATGAAGGAGAAGTAGTAAAGGAT
GTTACAGAACATTCAGCATT

Product: 3,4-dihydroxyphenylacetate 2,3-dioxygenase

Products: NA

Alternate protein names: Mn(II)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase; Mn(II)-dependent 23OHBP oxygenase [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MINILRTSHVCIRVSDLDKARYFYSELLGFVETEKDGDYIYLRGIEEGQHHSLILKKANSAGLSYIGFRVSKGEELDKAK
EELENLGHKVRKFREKGINEALLFESPEGIPILLYYEMEYVGDLRLAFHRHRGVSPVRLAHVNYMVKDLERELEFFKRFM
GYYETERFLDKDGNLTVVWLTRRGDSHEIAIAKSSKNVPGFHHETYYVHDVKDVIKAPDILASVGLWDSIERGPGRHGVT
EGYYIYLRDFDKNRIEFFTNDYVVLDPDKWKPIVWTYDQLRYRSDFWGRPIPQSWLTEWVPVEDISTGQLKGWSG

Sequences:

>Translated_315_residues
MINILRTSHVCIRVSDLDKARYFYSELLGFVETEKDGDYIYLRGIEEGQHHSLILKKANSAGLSYIGFRVSKGEELDKAK
EELENLGHKVRKFREKGINEALLFESPEGIPILLYYEMEYVGDLRLAFHRHRGVSPVRLAHVNYMVKDLERELEFFKRFM
GYYETERFLDKDGNLTVVWLTRRGDSHEIAIAKSSKNVPGFHHETYYVHDVKDVIKAPDILASVGLWDSIERGPGRHGVT
EGYYIYLRDFDKNRIEFFTNDYVVLDPDKWKPIVWTYDQLRYRSDFWGRPIPQSWLTEWVPVEDISTGQLKGWSG
>Mature_315_residues
MINILRTSHVCIRVSDLDKARYFYSELLGFVETEKDGDYIYLRGIEEGQHHSLILKKANSAGLSYIGFRVSKGEELDKAK
EELENLGHKVRKFREKGINEALLFESPEGIPILLYYEMEYVGDLRLAFHRHRGVSPVRLAHVNYMVKDLERELEFFKRFM
GYYETERFLDKDGNLTVVWLTRRGDSHEIAIAKSSKNVPGFHHETYYVHDVKDVIKAPDILASVGLWDSIERGPGRHGVT
EGYYIYLRDFDKNRIEFFTNDYVVLDPDKWKPIVWTYDQLRYRSDFWGRPIPQSWLTEWVPVEDISTGQLKGWSG

Specific function: Catalyzes the meta-cleavage of the hydroxylated biphenyl ring. The enzyme can oxidize a wide range of substrates, and the substrate preference order is 2,3-dihydroxybiphenyl > 3- methylcatechol > catechol > 4-methylcatechol > 4-chlorocatechol [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the extradiol ring-cleavage dioxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =1.13.11.39 [H]

Molecular weight: Translated: 36954; Mature: 36954

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINILRTSHVCIRVSDLDKARYFYSELLGFVETEKDGDYIYLRGIEEGQHHSLILKKANS
CEEEEEECEEEEEEECCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEECCC
AGLSYIGFRVSKGEELDKAKEELENLGHKVRKFREKGINEALLFESPEGIPILLYYEMEY
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEEEH
VGDLRLAFHRHRGVSPVRLAHVNYMVKDLERELEFFKRFMGYYETERFLDKDGNLTVVWL
HHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
TRRGDSHEIAIAKSSKNVPGFHHETYYVHDVKDVIKAPDILASVGLWDSIERGPGRHGVT
EECCCCCEEEEEECCCCCCCCCCCEEEEECHHHHHCCCHHHHHCCCHHHHHCCCCCCCCC
EGYYIYLRDFDKNRIEFFTNDYVVLDPDKWKPIVWTYDQLRYRSDFWGRPIPQSWLTEWV
CCEEEEEEECCCCCEEEEECCEEEECCCCCCCEEEEHHHHHHHHCCCCCCCCHHHHHCCC
PVEDISTGQLKGWSG
CCCCCCCCCCCCCCC
>Mature Secondary Structure
MINILRTSHVCIRVSDLDKARYFYSELLGFVETEKDGDYIYLRGIEEGQHHSLILKKANS
CEEEEEECEEEEEEECCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEECCC
AGLSYIGFRVSKGEELDKAKEELENLGHKVRKFREKGINEALLFESPEGIPILLYYEMEY
CCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEEEH
VGDLRLAFHRHRGVSPVRLAHVNYMVKDLERELEFFKRFMGYYETERFLDKDGNLTVVWL
HHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
TRRGDSHEIAIAKSSKNVPGFHHETYYVHDVKDVIKAPDILASVGLWDSIERGPGRHGVT
EECCCCCEEEEEECCCCCCCCCCCEEEEECHHHHHCCCHHHHHCCCHHHHHCCCCCCCCC
EGYYIYLRDFDKNRIEFFTNDYVVLDPDKWKPIVWTYDQLRYRSDFWGRPIPQSWLTEWV
CCEEEEEEECCCCCEEEEECCEEEECCCCCCCEEEEHHHHHHHHCCCCCCCCHHHHHCCC
PVEDISTGQLKGWSG
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12672826 [H]