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The map label for this gene is treZ [H]

Identifier: 227826851

GI number: 227826851

Start: 415494

End: 417179

Strand: Direct

Name: treZ [H]

Synonym: M1425_0477

Alternate gene names: 227826851

Gene position: 415494-417179 (Clockwise)

Preceding gene: 227826850

Following gene: 227826852

Centisome position: 15.93

GC content: 36.0

Gene sequence:

>1686_bases
ATGGAATTTGGTTATAGATTAGATAGGAACGGAGTAACCTTTAATTTATGGGCTCCCTATCAAAGAAACGTTAAGTTAAA
GATACTAAATAAGGGAATTTATGAGATGGAAAGAGACGAGAAGGGATACTTCACCATTACGTTAAATAACGTGAAAGTTG
GTGATAGATATAAGTATATTTTAGATGACGGTAGTGAAGTGCCAGATCCGGCTTCAAGGTATCAGCCAGAGGGAGTTCAT
GGCCATTCTGAAATTATTTCACTAGATTTTAAAGGGGATGATGAGAACGGTGTAAAGGTAAAAAGGGAAGATCTTATAAT
TTATGAACTTCATACAAGTACATTTACATCAGAAGGTACTTTTGAAGGAGTGATAAAGAAGCTGGACTACTTAAAAGAAC
TTGGAGTAACTGCAATAGAAATAATGCCTATTGCACAGTTTCCAGGGAAAAAGGACTGGGGTTATGATGGAGTTTACTTG
TACGCTGTACAGAACTCTTATGGAGGACCAATTGGGTTTAGAAATCTAGTAAATGAGGCTCATAAACTCGGATTAGCGAT
AATATTAGACGTAGTTTATAACCACGTTGGGCCCGAAGGGAATTACATGGCCAAGTTAGGGCCCTATTTCTCAGAGAAAT
ATAAAACACCTTGGGGTTTAGCATTTAATTTTGACGATGCTGGAAGTGATGAAGTTAGAAAGTTCATACTAGAAAATGTT
GAATATTGGATAAATGAGTTTCACGTTGATGGATTCAGACTTGATGCTGTTCACGCTATTATTGACAATTCTCCAAAACA
CATCTTAGAGGATATTGCTGATGTAGTCCACAAATATGGCAAGATTGTAATAACTGAGAGTGATTTAAATGATCCTAGAG
TTGTTAATCCTAAGGAGAAATGTGGATATAATACTGATGCCCAGTGGGTAGATGATTTCCATCATGCAATCCATGCGTTC
TTAACTGGTGAGAGGCAAGGTTATTACAGTGATTTCGGTAGTCTAGATGACATAGTTAAATCATACAAAGAAGTCTTCGT
ATATGATGGAAAGTACTCGAATTTTAGAAGAAAAACTCACGGAAAACCGGTTGGTGATCTAGATGGTTGTAAGTTCGTAG
TTTACATTCAAAATCACGATCAAGTTGGCAATAGGGGTGGAGGAGAGAGATTAATTAAACTAGTTGATAAGGAGAGTTAT
AAGATTGCTGCAGCACTCTACATGCTTTCACCATATATTCCGATGATCTTTATGGGAGAGGAATACGGTGAGGAAAATCC
GTTTTACTACTTTTCCGACTTTTCTGATCCTAAACTAATACAAGGGGTCAAGGAGGGTAGAAAAAGAGATAATGGTCAAG
AGACTGATCCACAGTCGGACTCCACATTTAATGCCTCTAAATTAAGCTGGAAGATAAATGATGACATTTTTTCATTTTAC
AAGACTTTAATAAAAATAAGGAAAGAATACGGTCTAGCTTGTAATAGGAAATTGACCGTTGAAAATGGCAATCATTGGTT
AACTGTGAAAGGAAATGGATATTTAGTAGTTTACGTGTTTTCCCAATCGATAATTGAAATGAAATATAGGGGGACTCTAG
TTTTATCCTCCAATAACTCTTTCCCATCACAAATTGTAGAGAATAAGTATAAATTAGAAAAGGGATTTGCTTTATATAAA
CTTTAG

Upstream 100 bases:

>100_bases
TCTAAAATAAGCTTGATCTTCTTACTCCTCTCATTGGCAATGATGATACTAGCTGGTTCAATAGCACAAGTTATAACTTA
GAAAGGAAGAAATTCATATT

Downstream 100 bases:

>100_bases
GGCAAGAGAGTTTAAAAATTCCTATGAACGATTATACTTTAGATGATAAGTAAAAATAAAATTGATGAGGAAGAGAAAAG
GAGAAGAGAAGAAGTTAAAA

Product: malto-oligosyltrehalose trehalohydrolase

Products: NA

Alternate protein names: MTHase; 4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase; Maltooligosyl trehalose trehalohydrolase [H]

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MEFGYRLDRNGVTFNLWAPYQRNVKLKILNKGIYEMERDEKGYFTITLNNVKVGDRYKYILDDGSEVPDPASRYQPEGVH
GHSEIISLDFKGDDENGVKVKREDLIIYELHTSTFTSEGTFEGVIKKLDYLKELGVTAIEIMPIAQFPGKKDWGYDGVYL
YAVQNSYGGPIGFRNLVNEAHKLGLAIILDVVYNHVGPEGNYMAKLGPYFSEKYKTPWGLAFNFDDAGSDEVRKFILENV
EYWINEFHVDGFRLDAVHAIIDNSPKHILEDIADVVHKYGKIVITESDLNDPRVVNPKEKCGYNTDAQWVDDFHHAIHAF
LTGERQGYYSDFGSLDDIVKSYKEVFVYDGKYSNFRRKTHGKPVGDLDGCKFVVYIQNHDQVGNRGGGERLIKLVDKESY
KIAAALYMLSPYIPMIFMGEEYGEENPFYYFSDFSDPKLIQGVKEGRKRDNGQETDPQSDSTFNASKLSWKINDDIFSFY
KTLIKIRKEYGLACNRKLTVENGNHWLTVKGNGYLVVYVFSQSIIEMKYRGTLVLSSNNSFPSQIVENKYKLEKGFALYK
L

Sequences:

>Translated_561_residues
MEFGYRLDRNGVTFNLWAPYQRNVKLKILNKGIYEMERDEKGYFTITLNNVKVGDRYKYILDDGSEVPDPASRYQPEGVH
GHSEIISLDFKGDDENGVKVKREDLIIYELHTSTFTSEGTFEGVIKKLDYLKELGVTAIEIMPIAQFPGKKDWGYDGVYL
YAVQNSYGGPIGFRNLVNEAHKLGLAIILDVVYNHVGPEGNYMAKLGPYFSEKYKTPWGLAFNFDDAGSDEVRKFILENV
EYWINEFHVDGFRLDAVHAIIDNSPKHILEDIADVVHKYGKIVITESDLNDPRVVNPKEKCGYNTDAQWVDDFHHAIHAF
LTGERQGYYSDFGSLDDIVKSYKEVFVYDGKYSNFRRKTHGKPVGDLDGCKFVVYIQNHDQVGNRGGGERLIKLVDKESY
KIAAALYMLSPYIPMIFMGEEYGEENPFYYFSDFSDPKLIQGVKEGRKRDNGQETDPQSDSTFNASKLSWKINDDIFSFY
KTLIKIRKEYGLACNRKLTVENGNHWLTVKGNGYLVVYVFSQSIIEMKYRGTLVLSSNNSFPSQIVENKYKLEKGFALYK
L
>Mature_561_residues
MEFGYRLDRNGVTFNLWAPYQRNVKLKILNKGIYEMERDEKGYFTITLNNVKVGDRYKYILDDGSEVPDPASRYQPEGVH
GHSEIISLDFKGDDENGVKVKREDLIIYELHTSTFTSEGTFEGVIKKLDYLKELGVTAIEIMPIAQFPGKKDWGYDGVYL
YAVQNSYGGPIGFRNLVNEAHKLGLAIILDVVYNHVGPEGNYMAKLGPYFSEKYKTPWGLAFNFDDAGSDEVRKFILENV
EYWINEFHVDGFRLDAVHAIIDNSPKHILEDIADVVHKYGKIVITESDLNDPRVVNPKEKCGYNTDAQWVDDFHHAIHAF
LTGERQGYYSDFGSLDDIVKSYKEVFVYDGKYSNFRRKTHGKPVGDLDGCKFVVYIQNHDQVGNRGGGERLIKLVDKESY
KIAAALYMLSPYIPMIFMGEEYGEENPFYYFSDFSDPKLIQGVKEGRKRDNGQETDPQSDSTFNASKLSWKINDDIFSFY
KTLIKIRKEYGLACNRKLTVENGNHWLTVKGNGYLVVYVFSQSIIEMKYRGTLVLSSNNSFPSQIVENKYKLEKGFALYK
L

Specific function: Catalyzes The Formation Of The Alpha-1,6-Glucosidic Linkages In Glycogen By Scission Of A 1,4-Alpha-Linked Oligosaccharide From Growing Alpha-1,4-Glucan Chains And The Subsequent Attachment Of The Oligosaccharide To The Alpha-1,6 Position. Has A Preferenc

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI189458812, Length=177, Percent_Identity=28.2485875706215, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1789839, Length=282, Percent_Identity=28.7234042553192, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI2367229, Length=533, Percent_Identity=23.0769230769231, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1790687, Length=423, Percent_Identity=24.3498817966903, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17554896, Length=177, Percent_Identity=29.9435028248588, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI32564391, Length=177, Percent_Identity=29.9435028248588, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320826, Length=298, Percent_Identity=26.510067114094, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI28573410, Length=177, Percent_Identity=25.4237288135593, Blast_Score=67, Evalue=4e-11,
Organism=Drosophila melanogaster, GI221330053, Length=87, Percent_Identity=43.6781609195402, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- InterPro:   IPR015156
- InterPro:   IPR012768 [H]

Pfam domain/function: PF09071 Alpha-amyl_C; PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: =3.2.1.141 [H]

Molecular weight: Translated: 64358; Mature: 64358

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFGYRLDRNGVTFNLWAPYQRNVKLKILNKGIYEMERDEKGYFTITLNNVKVGDRYKYI
CCCCCEECCCCEEEEEECCCCCCEEEEEECCCHHHHCCCCCCEEEEEECCEEECCEEEEE
LDDGSEVPDPASRYQPEGVHGHSEIISLDFKGDDENGVKVKREDLIIYELHTSTFTSEGT
ECCCCCCCCHHHHCCCCCCCCCCEEEEEEECCCCCCCCEEEECCEEEEEEECCCCCCCCH
FEGVIKKLDYLKELGVTAIEIMPIAQFPGKKDWGYDGVYLYAVQNSYGGPIGFRNLVNEA
HHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHH
HKLGLAIILDVVYNHVGPEGNYMAKLGPYFSEKYKTPWGLAFNFDDAGSDEVRKFILENV
HHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHH
EYWINEFHVDGFRLDAVHAIIDNSPKHILEDIADVVHKYGKIVITESDLNDPRVVNPKEK
HHHHHHHCCCCEEEHEEHHHHCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCEECCCHHH
CGYNTDAQWVDDFHHAIHAFLTGERQGYYSDFGSLDDIVKSYKEVFVYDGKYSNFRRKTH
CCCCCCHHHHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHHEEEECCCCCCCHHHHC
GKPVGDLDGCKFVVYIQNHDQVGNRGGGERLIKLVDKESYKIAAALYMLSPYIPMIFMGE
CCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEECH
EYGEENPFYYFSDFSDPKLIQGVKEGRKRDNGQETDPQSDSTFNASKLSWKINDDIFSFY
HHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECHHHHHHH
KTLIKIRKEYGLACNRKLTVENGNHWLTVKGNGYLVVYVFSQSIIEMKYRGTLVLSSNNS
HHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCEEEEEEECCCHHEEEECEEEEEECCCC
FPSQIVENKYKLEKGFALYKL
CHHHHHHHHHHHHCCEEEECC
>Mature Secondary Structure
MEFGYRLDRNGVTFNLWAPYQRNVKLKILNKGIYEMERDEKGYFTITLNNVKVGDRYKYI
CCCCCEECCCCEEEEEECCCCCCEEEEEECCCHHHHCCCCCCEEEEEECCEEECCEEEEE
LDDGSEVPDPASRYQPEGVHGHSEIISLDFKGDDENGVKVKREDLIIYELHTSTFTSEGT
ECCCCCCCCHHHHCCCCCCCCCCEEEEEEECCCCCCCCEEEECCEEEEEEECCCCCCCCH
FEGVIKKLDYLKELGVTAIEIMPIAQFPGKKDWGYDGVYLYAVQNSYGGPIGFRNLVNEA
HHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHH
HKLGLAIILDVVYNHVGPEGNYMAKLGPYFSEKYKTPWGLAFNFDDAGSDEVRKFILENV
HHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHH
EYWINEFHVDGFRLDAVHAIIDNSPKHILEDIADVVHKYGKIVITESDLNDPRVVNPKEK
HHHHHHHCCCCEEEHEEHHHHCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCEECCCHHH
CGYNTDAQWVDDFHHAIHAFLTGERQGYYSDFGSLDDIVKSYKEVFVYDGKYSNFRRKTH
CCCCCCHHHHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHHEEEECCCCCCCHHHHC
GKPVGDLDGCKFVVYIQNHDQVGNRGGGERLIKLVDKESYKIAAALYMLSPYIPMIFMGE
CCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEECH
EYGEENPFYYFSDFSDPKLIQGVKEGRKRDNGQETDPQSDSTFNASKLSWKINDDIFSFY
HHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECHHHHHHH
KTLIKIRKEYGLACNRKLTVENGNHWLTVKGNGYLVVYVFSQSIIEMKYRGTLVLSSNNS
HHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCEEEEEEECCCHHEEEECEEEEEECCCC
FPSQIVENKYKLEKGFALYKL
CHHHHHHHHHHHHCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8899719; 11427726 [H]