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The map label for this gene is glgX [H]

Identifier: 227826852

GI number: 227826852

Start: 417348

End: 419504

Strand: Direct

Name: glgX [H]

Synonym: M1425_0478

Alternate gene names: 227826852

Gene position: 417348-419504 (Clockwise)

Preceding gene: 227826851

Following gene: 227826853

Centisome position: 16.0

GC content: 36.35

Gene sequence:

>2157_bases
ATGTCAGTATTCTTCAGAACTAGGGATAGACCTCTACGTCCTGGAGATCCATATCCATTAGGTTCAAATTGGATAGAAGA
TGAGGATGGAGTAAATTTTTCCTTATTCTCAGAGAATGCAGAAAAAGTTGAACTACTCCTTTATTCACAAACAAATCAAA
AGTATCCAAAGGAGATAATAGAGGTTAAAAACAGAACGGGAGATCTTTGGCATATATTAGTCCCAGGGCTGAGGCCCGGG
CAACTTTACGCATACAAGGTTTATGGTCCGTATAAACCGGCGTTAGGATTGAGATTTAATCCTAATAAGGTTTTAATAGA
CCCTTACGCTAAAGCCATAAATGGTAATGTGATTTGGAACGATGCAGTATTTGGTTATAAGATAGGAGACCAAAATCAAG
ATTTAAGCTATGACGAGAGGGATTCCAGCGAATATGTACCTAAAAGTGTTGTGATTAACCCATACTTTGAATGGGACGAT
GAGGATTTCATTAAGAGGAATAAGGTTCCATTAAAGGATACAGTAATTTATGAAGTTCACGTAAAAGGATTTACAAAACT
TAGATTGGATTTGCCGGAAAACATAAGGGGATCTTATGAAGGTTTAGCCTCAGAACAAATGATTAGCTATCTCAAAGATT
TAGGGATTACTACTGTAGAATTAATGCCGGTTTTTCACTTCATTGATCAAAGATTTCTGATAGATAAGGGGCTAACAAAT
TACTGGGGATATGACCCAATAAACTTCTTCTCCCCGGAATGTAGATATTCCAGTAGCGGCTGTTTAGGAGAACAAGTATT
CAGTTTCAAGAAAATGGTAAACGAATTGCATAACGCAGGAATCGAGGTTATAATTGATGTAGTTTATAATCATACTGCTG
AGGGAAATCATTTAGGACCAACGTTAAGTTTCAGAGGTATTGATAATATAGCATATTATATGCTCCAACCAGACAATAAA
AGATATTATTTAGACTTTACCGGAACTGGCAACACGTTAAATCTTAGCCATCCAAGAGTTATCCAGATGGTCCTAGATAG
CTTAAGATATTGGGTTACTGATATGCACGTAGATGGCTTTAGATTTGACTTAGCAGCCGCTTTAGCTAGGGAATTGTACA
GCGTTAATATGTTAAATACGTTCTTCATTGCATTACAGCAAGATCCAATAGTTTCACGAGTAAAACTAATAGCTGAACCG
TGGGATGTAGGACAAGGAGGATATCAAGTTGGGAATTTCCCACATCAATGGGCAGAATGGAATGGAAAATATAGGGATTC
TATAAGGAGATTTTGGAGAGGAGAAGCGTTACCCTATAGCGAGATTGCTAACAGGCTATTAGGTTCACCAGACATCTACT
TAGGTAATAATAAAACACCATTTGCCAGTATAAACTACGTAACTTCGCACGATGGTTTCACATTAGAAGATTTAGTTAGT
TATAATCAAAAACATAATGAGGCGAATGGATTTAACAATCAAGATGGAATGAACGAGAACTACAGTTGGAATTGTGGTGC
AGAAGGAACTATAAATGACCAAAACGTAATTATGTGCAGAGAGAAACAGAAAAGGAACTTCATGATAACGTTACTTATTA
GCCAAGGAACTCCCATGATATTAGGAGGAGATGAGTTAAGTAGGACACAAAGAGGTAATAATAACGCGTTTTGCCAAGAC
AATGAGATAACTTGGTTTGATTGGAATTTAGATGAGAGAAAATCAAAATTTTTAGAGTTTGTTAAAAAAATGATCCAATT
TTATAAAGCGCATCCGGTATTCAGAAGGGAAAGATATTTTCAAGGAAAGAAATTATTCGGCATGCCATTAAAAGATGTAA
CATTTTACACTCCAGACGGTAAGGAGGTTGATGAGAAAACGTGGAATTCCCCAACGCAAACTGTTATCTTCGTGTTAGAG
GGGAGCGTAATGGATGAGATTAACATCTACGGAGAGAGAATTGCAGATGATTCATTCTTAATAATACTTAACGCAAATCC
CAATAACGTAAAAGTGAAGTTTCAAAAAGGTAAATGGGAACTAGTTGTTTGTTCTTATTTAAGAGAGATAAAACCAGAAG
AAAGAATTGTAGAAGGTGAGAAGGAATTGGAAATTGAGGGAAGAACTGCATTAGTTTATAGGAGGATAGAACTATGA

Upstream 100 bases:

>100_bases
GGAAGAGAAAAGGAGAAGAGAAGAAGTTAAAAGGTTAGTAATACTCTTATCAATGTTAAGATAAAGGTTTTTTTAAACTC
AAATAATAATAAATATCATT

Downstream 100 bases:

>100_bases
TAACAGGCACATATAGGCTGCAACTAAATAATGGATTCACTTTTTATGATGTAATAGAAAATTTGGATTACTTTAAGGAA
TTAGGAGTATCACACCTATA

Product: glycogen debranching enzyme GlgX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 718; Mature: 717

Protein sequence:

>718_residues
MSVFFRTRDRPLRPGDPYPLGSNWIEDEDGVNFSLFSENAEKVELLLYSQTNQKYPKEIIEVKNRTGDLWHILVPGLRPG
QLYAYKVYGPYKPALGLRFNPNKVLIDPYAKAINGNVIWNDAVFGYKIGDQNQDLSYDERDSSEYVPKSVVINPYFEWDD
EDFIKRNKVPLKDTVIYEVHVKGFTKLRLDLPENIRGSYEGLASEQMISYLKDLGITTVELMPVFHFIDQRFLIDKGLTN
YWGYDPINFFSPECRYSSSGCLGEQVFSFKKMVNELHNAGIEVIIDVVYNHTAEGNHLGPTLSFRGIDNIAYYMLQPDNK
RYYLDFTGTGNTLNLSHPRVIQMVLDSLRYWVTDMHVDGFRFDLAAALARELYSVNMLNTFFIALQQDPIVSRVKLIAEP
WDVGQGGYQVGNFPHQWAEWNGKYRDSIRRFWRGEALPYSEIANRLLGSPDIYLGNNKTPFASINYVTSHDGFTLEDLVS
YNQKHNEANGFNNQDGMNENYSWNCGAEGTINDQNVIMCREKQKRNFMITLLISQGTPMILGGDELSRTQRGNNNAFCQD
NEITWFDWNLDERKSKFLEFVKKMIQFYKAHPVFRRERYFQGKKLFGMPLKDVTFYTPDGKEVDEKTWNSPTQTVIFVLE
GSVMDEINIYGERIADDSFLIILNANPNNVKVKFQKGKWELVVCSYLREIKPEERIVEGEKELEIEGRTALVYRRIEL

Sequences:

>Translated_718_residues
MSVFFRTRDRPLRPGDPYPLGSNWIEDEDGVNFSLFSENAEKVELLLYSQTNQKYPKEIIEVKNRTGDLWHILVPGLRPG
QLYAYKVYGPYKPALGLRFNPNKVLIDPYAKAINGNVIWNDAVFGYKIGDQNQDLSYDERDSSEYVPKSVVINPYFEWDD
EDFIKRNKVPLKDTVIYEVHVKGFTKLRLDLPENIRGSYEGLASEQMISYLKDLGITTVELMPVFHFIDQRFLIDKGLTN
YWGYDPINFFSPECRYSSSGCLGEQVFSFKKMVNELHNAGIEVIIDVVYNHTAEGNHLGPTLSFRGIDNIAYYMLQPDNK
RYYLDFTGTGNTLNLSHPRVIQMVLDSLRYWVTDMHVDGFRFDLAAALARELYSVNMLNTFFIALQQDPIVSRVKLIAEP
WDVGQGGYQVGNFPHQWAEWNGKYRDSIRRFWRGEALPYSEIANRLLGSPDIYLGNNKTPFASINYVTSHDGFTLEDLVS
YNQKHNEANGFNNQDGMNENYSWNCGAEGTINDQNVIMCREKQKRNFMITLLISQGTPMILGGDELSRTQRGNNNAFCQD
NEITWFDWNLDERKSKFLEFVKKMIQFYKAHPVFRRERYFQGKKLFGMPLKDVTFYTPDGKEVDEKTWNSPTQTVIFVLE
GSVMDEINIYGERIADDSFLIILNANPNNVKVKFQKGKWELVVCSYLREIKPEERIVEGEKELEIEGRTALVYRRIEL
>Mature_717_residues
SVFFRTRDRPLRPGDPYPLGSNWIEDEDGVNFSLFSENAEKVELLLYSQTNQKYPKEIIEVKNRTGDLWHILVPGLRPGQ
LYAYKVYGPYKPALGLRFNPNKVLIDPYAKAINGNVIWNDAVFGYKIGDQNQDLSYDERDSSEYVPKSVVINPYFEWDDE
DFIKRNKVPLKDTVIYEVHVKGFTKLRLDLPENIRGSYEGLASEQMISYLKDLGITTVELMPVFHFIDQRFLIDKGLTNY
WGYDPINFFSPECRYSSSGCLGEQVFSFKKMVNELHNAGIEVIIDVVYNHTAEGNHLGPTLSFRGIDNIAYYMLQPDNKR
YYLDFTGTGNTLNLSHPRVIQMVLDSLRYWVTDMHVDGFRFDLAAALARELYSVNMLNTFFIALQQDPIVSRVKLIAEPW
DVGQGGYQVGNFPHQWAEWNGKYRDSIRRFWRGEALPYSEIANRLLGSPDIYLGNNKTPFASINYVTSHDGFTLEDLVSY
NQKHNEANGFNNQDGMNENYSWNCGAEGTINDQNVIMCREKQKRNFMITLLISQGTPMILGGDELSRTQRGNNNAFCQDN
EITWFDWNLDERKSKFLEFVKKMIQFYKAHPVFRRERYFQGKKLFGMPLKDVTFYTPDGKEVDEKTWNSPTQTVIFVLEG
SVMDEINIYGERIADDSFLIILNANPNNVKVKFQKGKWELVVCSYLREIKPEERIVEGEKELEIEGRTALVYRRIEL

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=670, Percent_Identity=41.6417910447761, Blast_Score=512, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 83269; Mature: 83138

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVFFRTRDRPLRPGDPYPLGSNWIEDEDGVNFSLFSENAEKVELLLYSQTNQKYPKEII
CCEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCCHHHH
EVKNRTGDLWHILVPGLRPGQLYAYKVYGPYKPALGLRFNPNKVLIDPYAKAINGNVIWN
HHHCCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCEEECCCEEEECCHHHHCCCCEEEE
DAVFGYKIGDQNQDLSYDERDSSEYVPKSVVINPYFEWDDEDFIKRNKVPLKDTVIYEVH
CCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHCCCCCCCCEEEEEEE
VKGFTKLRLDLPENIRGSYEGLASEQMISYLKDLGITTVELMPVFHFIDQRFLIDKGLTN
ECCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCC
YWGYDPINFFSPECRYSSSGCLGEQVFSFKKMVNELHNAGIEVIIDVVYNHTAEGNHLGP
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCCCCCC
TLSFRGIDNIAYYMLQPDNKRYYLDFTGTGNTLNLSHPRVIQMVLDSLRYWVTDMHVDGF
EEEECCCCCEEEEEECCCCCEEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHEEECCCH
RFDLAAALARELYSVNMLNTFFIALQQDPIVSRVKLIAEPWDVGQGGYQVGNFPHQWAEW
HHHHHHHHHHHHHHHHCEEEEEEEEECCCHHHHHHHHCCCCCCCCCCEECCCCCCHHHHC
NGKYRDSIRRFWRGEALPYSEIANRLLGSPDIYLGNNKTPFASINYVTSHDGFTLEDLVS
CCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEECCCCCCEEEEEEEECCCCCCHHHHHH
YNQKHNEANGFNNQDGMNENYSWNCGAEGTINDQNVIMCREKQKRNFMITLLISQGTPMI
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCEE
LGGDELSRTQRGNNNAFCQDNEITWFDWNLDERKSKFLEFVKKMIQFYKAHPVFRRERYF
ECCCHHHHHHCCCCCCEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
QGKKLFGMPLKDVTFYTPDGKEVDEKTWNSPTQTVIFVLEGSVMDEINIYGERIADDSFL
CCCEEECCCCHHEEEECCCCCCCCHHCCCCCCEEEEEEEECCCHHHHHHHHHEECCCCEE
IILNANPNNVKVKFQKGKWELVVCSYLREIKPEERIVEGEKELEIEGRTALVYRRIEL
EEEECCCCEEEEEEECCCEEEEHHHHHHHCCCHHHHHCCCCEEEECCCEEEEEEEECC
>Mature Secondary Structure 
SVFFRTRDRPLRPGDPYPLGSNWIEDEDGVNFSLFSENAEKVELLLYSQTNQKYPKEII
CEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEEECCCCCCCHHHH
EVKNRTGDLWHILVPGLRPGQLYAYKVYGPYKPALGLRFNPNKVLIDPYAKAINGNVIWN
HHHCCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCEEECCCEEEECCHHHHCCCCEEEE
DAVFGYKIGDQNQDLSYDERDSSEYVPKSVVINPYFEWDDEDFIKRNKVPLKDTVIYEVH
CCEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHCCCCCCCCEEEEEEE
VKGFTKLRLDLPENIRGSYEGLASEQMISYLKDLGITTVELMPVFHFIDQRFLIDKGLTN
ECCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCC
YWGYDPINFFSPECRYSSSGCLGEQVFSFKKMVNELHNAGIEVIIDVVYNHTAEGNHLGP
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCCCCCC
TLSFRGIDNIAYYMLQPDNKRYYLDFTGTGNTLNLSHPRVIQMVLDSLRYWVTDMHVDGF
EEEECCCCCEEEEEECCCCCEEEEEEECCCCEEECCCHHHHHHHHHHHHHHHHEEECCCH
RFDLAAALARELYSVNMLNTFFIALQQDPIVSRVKLIAEPWDVGQGGYQVGNFPHQWAEW
HHHHHHHHHHHHHHHHCEEEEEEEEECCCHHHHHHHHCCCCCCCCCCEECCCCCCHHHHC
NGKYRDSIRRFWRGEALPYSEIANRLLGSPDIYLGNNKTPFASINYVTSHDGFTLEDLVS
CCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEECCCCCCEEEEEEEECCCCCCHHHHHH
YNQKHNEANGFNNQDGMNENYSWNCGAEGTINDQNVIMCREKQKRNFMITLLISQGTPMI
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCEE
LGGDELSRTQRGNNNAFCQDNEITWFDWNLDERKSKFLEFVKKMIQFYKAHPVFRRERYF
ECCCHHHHHHCCCCCCEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
QGKKLFGMPLKDVTFYTPDGKEVDEKTWNSPTQTVIFVLEGSVMDEINIYGERIADDSFL
CCCEEECCCCHHEEEECCCCCCCCHHCCCCCCEEEEEEEECCCHHHHHHHHHEECCCCEE
IILNANPNNVKVKFQKGKWELVVCSYLREIKPEERIVEGEKELEIEGRTALVYRRIEL
EEEECCCCEEEEEEECCCEEEEHHHHHHHCCCHHHHHCCCCEEEECCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]