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The map label for this gene is blyA [H]

Identifier: 227815659

GI number: 227815659

Start: 2806189

End: 2806659

Strand: Reverse

Name: blyA [H]

Synonym: BAMEG_3078

Alternate gene names: 227815659

Gene position: 2806659-2806189 (Counterclockwise)

Preceding gene: 227815660

Following gene: 227815658

Centisome position: 53.66

GC content: 37.37

Gene sequence:

>471_bases
ATGGCGATGTTTCCTATAGAGCGTAACTATATTGGATATGGAAGTTCACGGTCGGGAATTCCTCTTTCTAAAGTAAGGTT
TATTGTAAGCCATGATACGGGGAATCCTGGTAGCAATGCGATTGGGAATCGGGATTATTTTAATGAGATACAACCGAAAG
CTTCGGCACATACATTTATAGATGACAAAACAATACTAGAAATTATTCCTATAAATGAAGTTGCTTATCATGTTCGATAT
GGTGTGCCGACAGATAATGACTTATATGGGTATGATGCCAATAAGGCTGCTATTGGAGTGGAGCTTTGTTATGGCGGTGA
TGTTAATTTTTGGGAAGCATATACCCGTTTTACTTGGTATCATGCGTATTTATGTCAAAACTTCGGCTTGAATCCGAAAA
AAGATATCGTATCACATAAAACACTTGATCCAGCCAGAAAGATTGATCCTGAAAATGTTTTAGGGAAATAA

Upstream 100 bases:

>100_bases
TTTGATTTTCTCTTCGCGTAAGAAATATGCTATAAGGACATTGCTACCGAATACATATATAAAGAAGTAAGGTAATCTTC
TGAAGCGGGAGTTGGTAGTA

Downstream 100 bases:

>100_bases
GGTATTAAATTTCAGCAGTTTTTAGCAGATGTCTATCGGATGTACGTTTCGTTTAGATGACAAATATATGAAAAATGAAT
ACAATAAAGATAGGTGCCTA

Product: N-acetylmuramoyl-L-alanine amidase, family 2

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MAMFPIERNYIGYGSSRSGIPLSKVRFIVSHDTGNPGSNAIGNRDYFNEIQPKASAHTFIDDKTILEIIPINEVAYHVRY
GVPTDNDLYGYDANKAAIGVELCYGGDVNFWEAYTRFTWYHAYLCQNFGLNPKKDIVSHKTLDPARKIDPENVLGK

Sequences:

>Translated_156_residues
MAMFPIERNYIGYGSSRSGIPLSKVRFIVSHDTGNPGSNAIGNRDYFNEIQPKASAHTFIDDKTILEIIPINEVAYHVRY
GVPTDNDLYGYDANKAAIGVELCYGGDVNFWEAYTRFTWYHAYLCQNFGLNPKKDIVSHKTLDPARKIDPENVLGK
>Mature_155_residues
AMFPIERNYIGYGSSRSGIPLSKVRFIVSHDTGNPGSNAIGNRDYFNEIQPKASAHTFIDDKTILEIIPINEVAYHVRYG
VPTDNDLYGYDANKAAIGVELCYGGDVNFWEAYTRFTWYHAYLCQNFGLNPKKDIVSHKTLDPARKIDPENVLGK

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Involved in prophage SP-beta-mediated cell lysis [H]

COG id: COG5632

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 17596; Mature: 17465

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMFPIERNYIGYGSSRSGIPLSKVRFIVSHDTGNPGSNAIGNRDYFNEIQPKASAHTFI
CCCCCCCCCEECCCCCCCCCCHHHEEEEEEECCCCCCCCCCCCCCHHHHCCCCCCCEEEE
DDKTILEIIPINEVAYHVRYGVPTDNDLYGYDANKAAIGVELCYGGDVNFWEAYTRFTWY
CCCEEEEEEECCCEEEEEEECCCCCCCEECCCCCCEEEEEEEEECCCCHHHHHHHHHHHH
HAYLCQNFGLNPKKDIVSHKTLDPARKIDPENVLGK
HHHHHHHCCCCCHHHHHCCCCCCHHHHCCCHHCCCC
>Mature Secondary Structure 
AMFPIERNYIGYGSSRSGIPLSKVRFIVSHDTGNPGSNAIGNRDYFNEIQPKASAHTFI
CCCCCCCCEECCCCCCCCCCHHHEEEEEEECCCCCCCCCCCCCCHHHHCCCCCCCEEEE
DDKTILEIIPINEVAYHVRYGVPTDNDLYGYDANKAAIGVELCYGGDVNFWEAYTRFTWY
CCCEEEEEEECCCEEEEEEECCCCCCCEECCCCCCEEEEEEEEECCCCHHHHHHHHHHHH
HAYLCQNFGLNPKKDIVSHKTLDPARKIDPENVLGK
HHHHHHHCCCCCHHHHHCCCCCCHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579063; 9384377 [H]