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The map label for this gene is appB [H]

Identifier: 227080800

GI number: 227080800

Start: 611946

End: 612932

Strand: Reverse

Name: appB [H]

Synonym: VCM66_0577

Alternate gene names: 227080800

Gene position: 612932-611946 (Counterclockwise)

Preceding gene: 227080801

Following gene: 227080799

Centisome position: 21.19

GC content: 48.33

Gene sequence:

>987_bases
ATGGGTTACTTTTTAAGACGATTGTCGTTCTATTTTGCCGCGTTGCTGGTCGCAGCAACATTAAACTTTATTATTCCGCG
TGCCATGCCTGGTGATCCGGTCACCATGATGTTTGCTCACGCAACCGCACAAGTTACTCCAGAACGTATCGAAGCCATGA
AACAGTTACTTGGCTTTGTTGAAGGTCCTTGGTATGTGCAGTATCTGACGTACATCAAAAGCATCCTGACTTGGGAGCTG
GGCACTTCAATCAAATTCTACCCTTTAAGTGTGAATGAGCTGCTTGGTGGTGCATTTGGTTGGTCACTGTTCCTTGCGGG
AACGGCGGTTATCATCTCTTTCTCAATCGGCTCTGTGCTGGGGATTTTTGCCGCATGGAAACGGGGCAGCCGTTACGACA
CTTTTATTACTCCGGGAATGCTGGTGATTCAGGCTGTACCACAAGTGGTTATTGCCATGCTCGCCATGTTCACTTTCGCT
ATCGGTTTAAGTTGGTTCCCAACTGGCTACGCATACACAGCAGGTACCACTCCAGATTGGACGAGTTGGGCATTCATCAA
AGACGTCGGTTATCACGCCGTTCTTCCTCTGTTCTGTGCTTCGATCGTGCAGATCGGTGGCTTCTTGGTCAACATGCGTA
ACAACATGATTAACCTGCTCGCTGAAGATTACATCACCATGGCAAAAGGCAAAGGCCTGAGCGAAAACCGCGTGGTATTT
AACTACGCAGCACGTAACGCTCTACTGCCAAGTGTGACCGCTCTTTCTATGTCGCTCGGTATGGCGATTGGTGGTCAGTT
GATTATCGAAATCATCTTCAACTACCCAGGTTTAGGCACTGTACTGTTCAACGCGATTACCGCACGTGACTACCAAGTTC
TGCAAGGTCAGCTGCTGATCATGACGCTGTTCATGCTGTTCTTTAACCTGCTGGCGGACATGTTATACGTTGTTCTTGAC
CCTCGCTTACGCAAGGGAGGTAAATAA

Upstream 100 bases:

>100_bases
ATATGGCGCTCGTCGTCAGGGGATTTCTCGCTCTCAAATTCGCTAGGGGCGAGACCTGAAACCAGAGACAGGACAAAACT
GGGTGAGTAAGGTGTAAGTT

Downstream 100 bases:

>100_bases
TCATGAAAGCACTTTGGAAACTCTTACGCGGCAACCGTATGGCGATGATTGGTGTCACCATCTTAGGCCTGTTTTTTCTG
CTCGCGGTCGCCGCACCACT

Product: peptide ABC transporter, permease protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MGYFLRRLSFYFAALLVAATLNFIIPRAMPGDPVTMMFAHATAQVTPERIEAMKQLLGFVEGPWYVQYLTYIKSILTWEL
GTSIKFYPLSVNELLGGAFGWSLFLAGTAVIISFSIGSVLGIFAAWKRGSRYDTFITPGMLVIQAVPQVVIAMLAMFTFA
IGLSWFPTGYAYTAGTTPDWTSWAFIKDVGYHAVLPLFCASIVQIGGFLVNMRNNMINLLAEDYITMAKGKGLSENRVVF
NYAARNALLPSVTALSMSLGMAIGGQLIIEIIFNYPGLGTVLFNAITARDYQVLQGQLLIMTLFMLFFNLLADMLYVVLD
PRLRKGGK

Sequences:

>Translated_328_residues
MGYFLRRLSFYFAALLVAATLNFIIPRAMPGDPVTMMFAHATAQVTPERIEAMKQLLGFVEGPWYVQYLTYIKSILTWEL
GTSIKFYPLSVNELLGGAFGWSLFLAGTAVIISFSIGSVLGIFAAWKRGSRYDTFITPGMLVIQAVPQVVIAMLAMFTFA
IGLSWFPTGYAYTAGTTPDWTSWAFIKDVGYHAVLPLFCASIVQIGGFLVNMRNNMINLLAEDYITMAKGKGLSENRVVF
NYAARNALLPSVTALSMSLGMAIGGQLIIEIIFNYPGLGTVLFNAITARDYQVLQGQLLIMTLFMLFFNLLADMLYVVLD
PRLRKGGK
>Mature_327_residues
GYFLRRLSFYFAALLVAATLNFIIPRAMPGDPVTMMFAHATAQVTPERIEAMKQLLGFVEGPWYVQYLTYIKSILTWELG
TSIKFYPLSVNELLGGAFGWSLFLAGTAVIISFSIGSVLGIFAAWKRGSRYDTFITPGMLVIQAVPQVVIAMLAMFTFAI
GLSWFPTGYAYTAGTTPDWTSWAFIKDVGYHAVLPLFCASIVQIGGFLVNMRNNMINLLAEDYITMAKGKGLSENRVVFN
YAARNALLPSVTALSMSLGMAIGGQLIIEIIFNYPGLGTVLFNAITARDYQVLQGQLLIMTLFMLFFNLLADMLYVVLDP
RLRKGGK

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789965, Length=339, Percent_Identity=30.9734513274336, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1787053, Length=326, Percent_Identity=29.4478527607362, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787497, Length=327, Percent_Identity=28.1345565749235, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1789888, Length=324, Percent_Identity=29.320987654321, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1788504, Length=365, Percent_Identity=26.3013698630137, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1787761, Length=333, Percent_Identity=27.3273273273273, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787550, Length=277, Percent_Identity=22.7436823104693, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 36262; Mature: 36130

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYFLRRLSFYFAALLVAATLNFIIPRAMPGDPVTMMFAHATAQVTPERIEAMKQLLGFV
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEHHHHCCCHHHHHHHHHHHHHH
EGPWYVQYLTYIKSILTWELGTSIKFYPLSVNELLGGAFGWSLFLAGTAVIISFSIGSVL
CCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIFAAWKRGSRYDTFITPGMLVIQAVPQVVIAMLAMFTFAIGLSWFPTGYAYTAGTTPDW
HHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCC
TSWAFIKDVGYHAVLPLFCASIVQIGGFLVNMRNNMINLLAEDYITMAKGKGLSENRVVF
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
NYAARNALLPSVTALSMSLGMAIGGQLIIEIIFNYPGLGTVLFNAITARDYQVLQGQLLI
EEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
MTLFMLFFNLLADMLYVVLDPRLRKGGK
HHHHHHHHHHHHHHHHHHHCHHHHCCCC
>Mature Secondary Structure 
GYFLRRLSFYFAALLVAATLNFIIPRAMPGDPVTMMFAHATAQVTPERIEAMKQLLGFV
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEHHHHCCCHHHHHHHHHHHHHH
EGPWYVQYLTYIKSILTWELGTSIKFYPLSVNELLGGAFGWSLFLAGTAVIISFSIGSVL
CCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIFAAWKRGSRYDTFITPGMLVIQAVPQVVIAMLAMFTFAIGLSWFPTGYAYTAGTTPDW
HHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCC
TSWAFIKDVGYHAVLPLFCASIVQIGGFLVNMRNNMINLLAEDYITMAKGKGLSENRVVF
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
NYAARNALLPSVTALSMSLGMAIGGQLIIEIIFNYPGLGTVLFNAITARDYQVLQGQLLI
EEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
MTLFMLFFNLLADMLYVVLDPRLRKGGK
HHHHHHHHHHHHHHHHHHHCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]