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The map label for this gene is pcnB [H]

Identifier: 227080777

GI number: 227080777

Start: 578319

End: 579692

Strand: Reverse

Name: pcnB [H]

Synonym: VCM66_0552

Alternate gene names: 227080777

Gene position: 579692-578319 (Counterclockwise)

Preceding gene: 227080778

Following gene: 227080776

Centisome position: 20.04

GC content: 51.82

Gene sequence:

>1374_bases
ATGAATAATAACGATTTTGAACCGAGCAACACTCGCACATATCCAGACTTAGCTTTGACCATCATTCCGCGTCAGGAACA
CCCTATCTCGCGTCAGCAGATCAGCGAAAATGCGCTGAAAGTGCTCTATCGTCTGCATGGTGCGGGGTTTGAGGCCTTTC
TGGTCGGCGGCGGTGTGCGTGATTTACTTCTGGGTGGCAAGCCGAAAGACTTTGATGTGGCCACCAACGCAACCCCCGAG
CAGTTACGCCAACTGTTTCGCAACTGCCGTCTGATTGGTCGCCGTTTCCGTCTCGCGCACATTATGTTTGGACGCGACAT
CGTCGAAGTCGCCACTTTCCGTGGCCATCATCAAGAGTCGAGTCAGAGCCAAAATATCGCTGCACAATCCGAAGCGGGTA
TGCTGCTACGTGATAACGTGTACGGCACGATTGATGAAGATGCCGAGCGCCGCGATTTCACCATCAATGCCATGTATTAC
AACATTGCAGACTACAGCATCCACGACTATGCCGGCGGCATGGAAGATCTGGAAGATCGTTTGGTGCGCCTGATTGGCGA
TCCGGAAACGCGTTATCGTGAAGATCCGGTACGCATGCTGCGCGCGGTACGTTTTGCGGTCAAACTCGATTTTGATATCG
AAGAAGATACCGCGGCGCCGATTGAGCAACTTGCGCCATTGCTGCGTGACATCCCTGCGGCTCGTCTCTACGAAGAATCG
CTCAAAATGCTGCAAGCGGGTCATGGTTTAGAAACCTACCACTTAATGCGTCAATACAATCTGTTCCAGCAACTTTTCCC
AACTATAGCCGAGCATTTTACTGCCGATCACAGCTCAAAAACTGAGCAGATGTTGGATTTGGTGCTGGATGCGACCGACA
TTCGGGTTGAAGAAGATCTGCGCATCAATCCGGCCTTTATGTTTGCCGCCATGCTGTGGTATCCGATGATTGATCTCGCG
GATCGCTTAATGGAATCGCTCGATCTCAATGAGTTTGATGCCATTACCGAAGCCAGCAACCGCATCTTAGATGAAGTGGT
CAAGCGTATCGCTATTCCGCGCCGCCACACCACTACAGTTCGTGATATTTGGCAGTTGCAACAGCGCTTACCCCGTCGCA
GTGGTAAAAGGGCCTTCCGTCTACTTGAACTGAACAAGTTCCGTGCAGGCTTTGATTTCCTCGAAATGCGTGGCGAAATT
GAAGGCGGTGAAACCAAACAATTAGCGGAATGGTGGGCCACCTTCCAAAGCGCGGGACGCAATATGCGCCAAGCCATGGT
GGGTGATATCGGTAATGCAACGGGCAGCAGCAAATCTGGCGCTCGCCGCCGTAAACCATCCCGCAACAAATCCAAGAGTA
AATCCAGCGAATGA

Upstream 100 bases:

>100_bases
TCCTGCTTAAGCTATTATTAGCGCCAAATTCCGTCCGTTTGAGCGTCAAATTAAGCAGCGTAGCGCTTACAGCGGCAACA
GCAATCATTGTCAGAAGCAC

Downstream 100 bases:

>100_bases
TGCTTGCCTACATTGCGATTGGCAGTAATTTGGGCGACCCTGTCGCCCAAGCACAGCTGGCCATACAACAGCTTACTCTC
TTACCGAAGTCGCGCCTTAT

Product: polyA polymerase

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MNNNDFEPSNTRTYPDLALTIIPRQEHPISRQQISENALKVLYRLHGAGFEAFLVGGGVRDLLLGGKPKDFDVATNATPE
QLRQLFRNCRLIGRRFRLAHIMFGRDIVEVATFRGHHQESSQSQNIAAQSEAGMLLRDNVYGTIDEDAERRDFTINAMYY
NIADYSIHDYAGGMEDLEDRLVRLIGDPETRYREDPVRMLRAVRFAVKLDFDIEEDTAAPIEQLAPLLRDIPAARLYEES
LKMLQAGHGLETYHLMRQYNLFQQLFPTIAEHFTADHSSKTEQMLDLVLDATDIRVEEDLRINPAFMFAAMLWYPMIDLA
DRLMESLDLNEFDAITEASNRILDEVVKRIAIPRRHTTTVRDIWQLQQRLPRRSGKRAFRLLELNKFRAGFDFLEMRGEI
EGGETKQLAEWWATFQSAGRNMRQAMVGDIGNATGSSKSGARRRKPSRNKSKSKSSE

Sequences:

>Translated_457_residues
MNNNDFEPSNTRTYPDLALTIIPRQEHPISRQQISENALKVLYRLHGAGFEAFLVGGGVRDLLLGGKPKDFDVATNATPE
QLRQLFRNCRLIGRRFRLAHIMFGRDIVEVATFRGHHQESSQSQNIAAQSEAGMLLRDNVYGTIDEDAERRDFTINAMYY
NIADYSIHDYAGGMEDLEDRLVRLIGDPETRYREDPVRMLRAVRFAVKLDFDIEEDTAAPIEQLAPLLRDIPAARLYEES
LKMLQAGHGLETYHLMRQYNLFQQLFPTIAEHFTADHSSKTEQMLDLVLDATDIRVEEDLRINPAFMFAAMLWYPMIDLA
DRLMESLDLNEFDAITEASNRILDEVVKRIAIPRRHTTTVRDIWQLQQRLPRRSGKRAFRLLELNKFRAGFDFLEMRGEI
EGGETKQLAEWWATFQSAGRNMRQAMVGDIGNATGSSKSGARRRKPSRNKSKSKSSE
>Mature_457_residues
MNNNDFEPSNTRTYPDLALTIIPRQEHPISRQQISENALKVLYRLHGAGFEAFLVGGGVRDLLLGGKPKDFDVATNATPE
QLRQLFRNCRLIGRRFRLAHIMFGRDIVEVATFRGHHQESSQSQNIAAQSEAGMLLRDNVYGTIDEDAERRDFTINAMYY
NIADYSIHDYAGGMEDLEDRLVRLIGDPETRYREDPVRMLRAVRFAVKLDFDIEEDTAAPIEQLAPLLRDIPAARLYEES
LKMLQAGHGLETYHLMRQYNLFQQLFPTIAEHFTADHSSKTEQMLDLVLDATDIRVEEDLRINPAFMFAAMLWYPMIDLA
DRLMESLDLNEFDAITEASNRILDEVVKRIAIPRRHTTTVRDIWQLQQRLPRRSGKRAFRLLELNKFRAGFDFLEMRGEI
EGGETKQLAEWWATFQSAGRNMRQAMVGDIGNATGSSKSGARRRKPSRNKSKSKSSE

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=217, Percent_Identity=32.7188940092166, Blast_Score=93, Evalue=6e-19,
Organism=Escherichia coli, GI87081691, Length=413, Percent_Identity=58.5956416464891, Blast_Score=527, Evalue=1e-151,
Organism=Escherichia coli, GI1789436, Length=240, Percent_Identity=28.3333333333333, Blast_Score=72, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6321016, Length=190, Percent_Identity=26.8421052631579, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI281360112, Length=268, Percent_Identity=27.6119402985075, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21357337, Length=268, Percent_Identity=27.6119402985075, Blast_Score=96, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 52501; Mature: 52501

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNNDFEPSNTRTYPDLALTIIPRQEHPISRQQISENALKVLYRLHGAGFEAFLVGGGVR
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCHH
DLLLGGKPKDFDVATNATPEQLRQLFRNCRLIGRRFRLAHIMFGRDIVEVATFRGHHQES
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SQSQNIAAQSEAGMLLRDNVYGTIDEDAERRDFTINAMYYNIADYSIHDYAGGMEDLEDR
HHHHCCHHHHHCCEEEECCCCCCCCCCCCCCCEEEEEEEEEEECCCHHHHCCCHHHHHHH
LVRLIGDPETRYREDPVRMLRAVRFAVKLDFDIEEDTAAPIEQLAPLLRDIPAARLYEES
HHHHHCCCHHHHCCCHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHH
LKMLQAGHGLETYHLMRQYNLFQQLFPTIAEHFTADHSSKTEQMLDLVLDATDIRVEEDL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEECCCC
RINPAFMFAAMLWYPMIDLADRLMESLDLNEFDAITEASNRILDEVVKRIAIPRRHTTTV
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
RDIWQLQQRLPRRSGKRAFRLLELNKFRAGFDFLEMRGEIEGGETKQLAEWWATFQSAGR
HHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
NMRQAMVGDIGNATGSSKSGARRRKPSRNKSKSKSSE
HHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MNNNDFEPSNTRTYPDLALTIIPRQEHPISRQQISENALKVLYRLHGAGFEAFLVGGGVR
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCHH
DLLLGGKPKDFDVATNATPEQLRQLFRNCRLIGRRFRLAHIMFGRDIVEVATFRGHHQES
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SQSQNIAAQSEAGMLLRDNVYGTIDEDAERRDFTINAMYYNIADYSIHDYAGGMEDLEDR
HHHHCCHHHHHCCEEEECCCCCCCCCCCCCCCEEEEEEEEEEECCCHHHHCCCHHHHHHH
LVRLIGDPETRYREDPVRMLRAVRFAVKLDFDIEEDTAAPIEQLAPLLRDIPAARLYEES
HHHHHCCCHHHHCCCHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHCCHHHHHHHH
LKMLQAGHGLETYHLMRQYNLFQQLFPTIAEHFTADHSSKTEQMLDLVLDATDIRVEEDL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEECCCC
RINPAFMFAAMLWYPMIDLADRLMESLDLNEFDAITEASNRILDEVVKRIAIPRRHTTTV
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
RDIWQLQQRLPRRSGKRAFRLLELNKFRAGFDFLEMRGEIEGGETKQLAEWWATFQSAGR
HHHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
NMRQAMVGDIGNATGSSKSGARRRKPSRNKSKSKSSE
HHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]