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The map label for this gene is gluQ

Identifier: 227080778

GI number: 227080778

Start: 579783

End: 580673

Strand: Reverse

Name: gluQ

Synonym: VCM66_0553

Alternate gene names: 227080778

Gene position: 580673-579783 (Counterclockwise)

Preceding gene: 227080779

Following gene: 227080777

Centisome position: 20.07

GC content: 50.84

Gene sequence:

>891_bases
ATGAACTATATTGGTCGCTTCGCCCCCTCTCCTTCTGGTCCATTACATTTTGGTTCTTTGATTGCGGCGTTGGGAAGCTA
TTTCCAAGCCAAAGCACAACAAGGAACTTGGCTGGTCCGTATTGAAGATCTCGATCCGCCCCGGGAAATGCCGGGAGCAG
CCGCGCACATTTTGCGTACATTAGAAGCCTACCGCCTGCACTGGGATGGCACTGTGGTTTACCAAAGCCAGCGTCACGCC
TTATACCAAGATCAAATCGATGCTTGGCTAAGCACAGGTCAAGCTTACTACTGCCAGTGCACCCGAAAAGCCATCAAAGC
CATGGGCGGCTTTTATAACGGCCACTGCAAAATCCATCCGCCAGCGCGGCAGCAAGACTGCTCGATTCGGCTACGAATGG
ATAATCAGGTGCAACAATTCATTGATTTGAAACACGGCACCATCACCATACCCAAGGCATTAGCAGAAGAAGATTTCATT
ATTAAACGCCGAGATGGTCTGTTTGCTTACAATCTTGCTGTGGTGCTGGATGATATTGATCAAGGCGTGACTCAGGTGGT
ACGCGGCGCCGATTTGATAGAACCGACAGGGCGGCAAATCAGCTTGTATCACACTTTAAAACAAAAGCCGGTCAGCTACT
TACATTTGCCTTTGGCGATGGATGGGAACGGGAATAAACTCTCGAAACAGAATCACGCTCCGGCCATTGACCCAACCGCG
CCTCGGCAGACCATTTCCCATGCGATGCAGTTTTTAGGCTTTACGCTGCCCACGGATTTTTCCGATGCCAGCGCAGAGCA
AATGCTGGCTTGGGGCTGCCAACATTGGCAGGTGAGCCAGCTACCGGAAGCGATCGAGATCACACCAAGATTCTCAAATG
GTCCTGCTTAA

Upstream 100 bases:

>100_bases
ATCAAAGAAAAGCAAATGCTTGGCTAAGATGGAACAGTAAGGAAAAGGGAGCACAAGCTCCCTTTTCTTGTTTCTTTGCA
TTTGAAAGGTAAATCGCCCT

Downstream 100 bases:

>100_bases
GCTATTATTAGCGCCAAATTCCGTCCGTTTGAGCGTCAAATTAAGCAGCGTAGCGCTTACAGCGGCAACAGCAATCATTG
TCAGAAGCACATGAATAATA

Product: glutamyl-Q tRNA(Asp) synthetase

Products: NA

Alternate protein names: Glu-Q-RSs

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MNYIGRFAPSPSGPLHFGSLIAALGSYFQAKAQQGTWLVRIEDLDPPREMPGAAAHILRTLEAYRLHWDGTVVYQSQRHA
LYQDQIDAWLSTGQAYYCQCTRKAIKAMGGFYNGHCKIHPPARQQDCSIRLRMDNQVQQFIDLKHGTITIPKALAEEDFI
IKRRDGLFAYNLAVVLDDIDQGVTQVVRGADLIEPTGRQISLYHTLKQKPVSYLHLPLAMDGNGNKLSKQNHAPAIDPTA
PRQTISHAMQFLGFTLPTDFSDASAEQMLAWGCQHWQVSQLPEAIEITPRFSNGPA

Sequences:

>Translated_296_residues
MNYIGRFAPSPSGPLHFGSLIAALGSYFQAKAQQGTWLVRIEDLDPPREMPGAAAHILRTLEAYRLHWDGTVVYQSQRHA
LYQDQIDAWLSTGQAYYCQCTRKAIKAMGGFYNGHCKIHPPARQQDCSIRLRMDNQVQQFIDLKHGTITIPKALAEEDFI
IKRRDGLFAYNLAVVLDDIDQGVTQVVRGADLIEPTGRQISLYHTLKQKPVSYLHLPLAMDGNGNKLSKQNHAPAIDPTA
PRQTISHAMQFLGFTLPTDFSDASAEQMLAWGCQHWQVSQLPEAIEITPRFSNGPA
>Mature_296_residues
MNYIGRFAPSPSGPLHFGSLIAALGSYFQAKAQQGTWLVRIEDLDPPREMPGAAAHILRTLEAYRLHWDGTVVYQSQRHA
LYQDQIDAWLSTGQAYYCQCTRKAIKAMGGFYNGHCKIHPPARQQDCSIRLRMDNQVQQFIDLKHGTITIPKALAEEDFI
IKRRDGLFAYNLAVVLDDIDQGVTQVVRGADLIEPTGRQISLYHTLKQKPVSYLHLPLAMDGNGNKLSKQNHAPAIDPTA
PRQTISHAMQFLGFTLPTDFSDASAEQMLAWGCQHWQVSQLPEAIEITPRFSNGPA

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily

Homologues:

Organism=Homo sapiens, GI134288884, Length=249, Percent_Identity=34.5381526104418, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI226510925, Length=295, Percent_Identity=56.9491525423729, Blast_Score=328, Evalue=3e-91,
Organism=Escherichia coli, GI1788743, Length=256, Percent_Identity=32.8125, Blast_Score=129, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71993394, Length=250, Percent_Identity=30.8, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6324540, Length=291, Percent_Identity=28.5223367697594, Blast_Score=124, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24665321, Length=255, Percent_Identity=29.8039215686275, Blast_Score=110, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLUQ_VIBCH (Q9KUC7)

Other databases:

- EMBL:   AE003852
- PIR:   D82304
- RefSeq:   NP_230245.1
- ProteinModelPortal:   Q9KUC7
- SMR:   Q9KUC7
- GeneID:   2615383
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0595
- TIGR:   VC_0595
- HOGENOM:   HBG628189
- OMA:   IIKRSDG
- ProtClustDB:   PRK05710
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01428
- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR03838

Pfam domain/function: PF00749 tRNA-synt_1c

EC number: NA

Molecular weight: Translated: 33109; Mature: 33109

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 50-50 BINDING 178-178 BINDING 196-196 BINDING 237-237

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYIGRFAPSPSGPLHFGSLIAALGSYFQAKAQQGTWLVRIEDLDPPREMPGAAAHILRT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHCCHHHHHHHHH
LEAYRLHWDGTVVYQSQRHALYQDQIDAWLSTGQAYYCQCTRKAIKAMGGFYNGHCKIHP
HHHHEEECCCEEEEECCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCCEEEECC
PARQQDCSIRLRMDNQVQQFIDLKHGTITIPKALAEEDFIIKRRDGLFAYNLAVVLDDID
CCCCCCCEEEEEECHHHHHHHHCCCCEEECCHHHCCCCCEEEECCCEEEEEHHHHHHHHH
QGVTQVVRGADLIEPTGRQISLYHTLKQKPVSYLHLPLAMDGNGNKLSKQNHAPAIDPTA
HHHHHHHHCCCCCCCCCCEEEEEEHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCC
PRQTISHAMQFLGFTLPTDFSDASAEQMLAWGCQHWQVSQLPEAIEITPRFSNGPA
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCHHHCCCEEEECCCCCCCCC
>Mature Secondary Structure
MNYIGRFAPSPSGPLHFGSLIAALGSYFQAKAQQGTWLVRIEDLDPPREMPGAAAHILRT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHCCHHHHHHHHH
LEAYRLHWDGTVVYQSQRHALYQDQIDAWLSTGQAYYCQCTRKAIKAMGGFYNGHCKIHP
HHHHEEECCCEEEEECCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCCEEEECC
PARQQDCSIRLRMDNQVQQFIDLKHGTITIPKALAEEDFIIKRRDGLFAYNLAVVLDDID
CCCCCCCEEEEEECHHHHHHHHCCCCEEECCHHHCCCCCEEEECCCEEEEEHHHHHHHHH
QGVTQVVRGADLIEPTGRQISLYHTLKQKPVSYLHLPLAMDGNGNKLSKQNHAPAIDPTA
HHHHHHHHCCCCCCCCCCEEEEEEHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCC
PRQTISHAMQFLGFTLPTDFSDASAEQMLAWGCQHWQVSQLPEAIEITPRFSNGPA
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCHHHCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301