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The map label for this gene is panB

Identifier: 227080775

GI number: 227080775

Start: 577022

End: 577816

Strand: Reverse

Name: panB

Synonym: VCM66_0550

Alternate gene names: 227080775

Gene position: 577816-577022 (Counterclockwise)

Preceding gene: 227080776

Following gene: 227080774

Centisome position: 19.98

GC content: 48.93

Gene sequence:

>795_bases
ATGAAAAAAATTACCATAAACGACCTGATGAAATGGAAGCAGGAAGGTCGCAAATTTGCTACATCAACCGCTTATGATGC
GAGTTTTGCTCAGTTATTTGAGAGCCAAGAAATGCCCGTCTTATTGGTCGGTGATTCACTTGGCATGGTGTTACAAGGAG
AAACCGACACCTTACCCGTGACGGTGGATGACATTGCGTATCACACCCGCTGTGTGCGTAAAGGCAGCCCCAATTGTCTG
CTGATGGCTGACATGCCTTTCATGAGCTACGCCACACCAGAACAAGCGTGTGAAAACGCGGCCAAACTGGTTCGCGCCGG
TGCAAACATGGTGAAAATTGAAGGTGGCGATTGGTTAGTCGACACCGTAAAAATGTTGACTGAACGTGCAGTGCCTGTGT
GTGCTCACCTCGGTTTAACGCCGCAGTCGGTCAATATTTTTGGTGGCTACAAAGTACAAGGCCGCGAGCAAGATAAAGCG
GATCGCATGGTACGTGATGCGTTGGCTCTGCAAGAAGCGGGCGCACAAATTGTCCTGCTAGAGTGCGTTCCAGCCGAACT
CGCCAACCGAATTACTCAAATTTTGGATGTTCCCGTAATAGGTATTGGCGCGGGTAATGGTACTGACGGCCAAATTTTGG
TGATGCACGATATGTTTGGAATTTCGGCGAACTACATGCCAAAATTCTCGAAAAATTTCTTGGCTGAGACTGGCGATATT
CGCCAAGCCGTTGCCAAATATATCGAAGATGTTGCCAGTGGCGCGTTTCCCGATCTCGCCCACACCATTGCCTAA

Upstream 100 bases:

>100_bases
TAACCCTCCCAGACGGGACTGAAATCCAAGCGTTATTGCAACAAGTACCGCTCAATGGGCTCAGCATTTGGCACTCGCCA
ACGTCGAGTAAGGACTAAAC

Downstream 100 bases:

>100_bases
GGAGTCATACATGCAAGTTTTTGCTGACATCGCCGTTCTTCGCGAGCAGATCAAACAGATCAAGCGCGAAGGTCGCCGCG
TCGCTTTTGTACCGACCATG

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKKITINDLMKWKQEGRKFATSTAYDASFAQLFESQEMPVLLVGDSLGMVLQGETDTLPVTVDDIAYHTRCVRKGSPNCL
LMADMPFMSYATPEQACENAAKLVRAGANMVKIEGGDWLVDTVKMLTERAVPVCAHLGLTPQSVNIFGGYKVQGREQDKA
DRMVRDALALQEAGAQIVLLECVPAELANRITQILDVPVIGIGAGNGTDGQILVMHDMFGISANYMPKFSKNFLAETGDI
RQAVAKYIEDVASGAFPDLAHTIA

Sequences:

>Translated_264_residues
MKKITINDLMKWKQEGRKFATSTAYDASFAQLFESQEMPVLLVGDSLGMVLQGETDTLPVTVDDIAYHTRCVRKGSPNCL
LMADMPFMSYATPEQACENAAKLVRAGANMVKIEGGDWLVDTVKMLTERAVPVCAHLGLTPQSVNIFGGYKVQGREQDKA
DRMVRDALALQEAGAQIVLLECVPAELANRITQILDVPVIGIGAGNGTDGQILVMHDMFGISANYMPKFSKNFLAETGDI
RQAVAKYIEDVASGAFPDLAHTIA
>Mature_264_residues
MKKITINDLMKWKQEGRKFATSTAYDASFAQLFESQEMPVLLVGDSLGMVLQGETDTLPVTVDDIAYHTRCVRKGSPNCL
LMADMPFMSYATPEQACENAAKLVRAGANMVKIEGGDWLVDTVKMLTERAVPVCAHLGLTPQSVNIFGGYKVQGREQDKA
DRMVRDALALQEAGAQIVLLECVPAELANRITQILDVPVIGIGAGNGTDGQILVMHDMFGISANYMPKFSKNFLAETGDI
RQAVAKYIEDVASGAFPDLAHTIA

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=263, Percent_Identity=68.8212927756654, Blast_Score=387, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6319653, Length=267, Percent_Identity=35.5805243445693, Blast_Score=162, Evalue=4e-41,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_VIBC3 (A5F975)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F975
- SMR:   A5F975
- STRING:   A5F975
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0123
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- ProtClustDB:   PRK00311
- BioCyc:   VCHO345073:VC0395_A0123-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 28659; Mature: 28659

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: ACT_SITE 181-181 BINDING 84-84 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKITINDLMKWKQEGRKFATSTAYDASFAQLFESQEMPVLLVGDSLGMVLQGETDTLPV
CCEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEECCCCCEEEECCCCCCCE
TVDDIAYHTRCVRKGSPNCLLMADMPFMSYATPEQACENAAKLVRAGANMVKIEGGDWLV
EHHHHHHHHHHHHCCCCCEEEEECCCCHHCCCHHHHHHHHHHHHHCCCCEEEECCCCHHH
DTVKMLTERAVPVCAHLGLTPQSVNIFGGYKVQGREQDKADRMVRDALALQEAGAQIVLL
HHHHHHHHHCCHHHHHCCCCCCCEEEEECEEECCCCHHHHHHHHHHHHHHHHCCCEEEEE
ECVPAELANRITQILDVPVIGIGAGNGTDGQILVMHDMFGISANYMPKFSKNFLAETGDI
EECCHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCHH
RQAVAKYIEDVASGAFPDLAHTIA
HHHHHHHHHHHHCCCCCHHHHHCC
>Mature Secondary Structure
MKKITINDLMKWKQEGRKFATSTAYDASFAQLFESQEMPVLLVGDSLGMVLQGETDTLPV
CCEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEECCCCCEEEECCCCCCCE
TVDDIAYHTRCVRKGSPNCLLMADMPFMSYATPEQACENAAKLVRAGANMVKIEGGDWLV
EHHHHHHHHHHHHCCCCCEEEEECCCCHHCCCHHHHHHHHHHHHHCCCCEEEECCCCHHH
DTVKMLTERAVPVCAHLGLTPQSVNIFGGYKVQGREQDKADRMVRDALALQEAGAQIVLL
HHHHHHHHHCCHHHHHCCCCCCCEEEEECEEECCCCHHHHHHHHHHHHHHHHCCCEEEEE
ECVPAELANRITQILDVPVIGIGAGNGTDGQILVMHDMFGISANYMPKFSKNFLAETGDI
EECCHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHCCCHH
RQAVAKYIEDVASGAFPDLAHTIA
HHHHHHHHHHHHCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA