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The map label for this gene is pykF [H]

Identifier: 227080695

GI number: 227080695

Start: 499501

End: 500913

Strand: Reverse

Name: pykF [H]

Synonym: VCM66_0470

Alternate gene names: 227080695

Gene position: 500913-499501 (Counterclockwise)

Preceding gene: 227080698

Following gene: 227080691

Centisome position: 17.32

GC content: 47.56

Gene sequence:

>1413_bases
ATGAAAAAGACCAAAATCGTATGTACGATTGGCCCAAAAACTGAGTCTGTAGAGAAACTGACTGAGCTTGTAAACGCTGG
CATGAATGTTATGCGTCTCAACTTCTCTCACGGTGACTACGTTGAACACGGCACTCGTATCACTAACTTCCGTAAAGTGA
TGGAAGTGACCGGTAAACAACTGGCGATCCTACTGGATACTAAAGGTCCAGAGATCCGTACTATCAAGCTGGAAAACGGT
GACGATGTGGATCTGGTCGCTGGCCAAGAGTTCACTTTCACAACTGACACGAAAGTTGTTGGTAACAAAGAGCGCGTAGC
AGTCACTTACTCTGGTTTTGCAAAAGACCTGAACGTGGGCAACCGTATTCTGGTTGACGACGGTCTTATAGAGATGGAAG
TACTGGCGACCACTGACACTGAAGTGAAGTGTAAAGTTCTGAACAACGGCGCTCTGGGTGAAAACAAAGGTGTTAACCTG
CCAGGCGTTTCTGTCAATCTGCCAGCGCTGTCTGAAAAAGACAAAAACGACCTGAAATTTGGTTGTGAGCAAGGCGTTGA
TTTCGTTGCTGCCTCTTTCATCCGTAAAGGTTCTGACGTGAAAGAAATCCGTGAAGTATTAGCTTCTCACGGCGGTCAAA
ACATTCAAATCATCTCTAAAATCGAAAACCAAGAAGGCTTGGACAACTTCGATGAGATCCTTGAGCTGTCTGACGGCATC
ATGGTTGCACGTGGCGACCTAGGTGTAGAAATCCCAGCAGAAGAAGTTATCTTCGCTCAAAAGATGATGATCGAGAAATG
TAACCGTGCACGTAAAGTGGTTATCACGGCGACGCAAATGCTGGATTCAATGATCAAGAACCCACGTCCAACTCGCGCAG
AAGCGGGCGACGTTGCGAACGCGATCATGGATGGTACTGATGCTGTAATGCTTTCTGGCGAAACAGCAAAAGGTAAGTAC
CCAGTTGAAGCGGTGAAGATCATGGCGCAAATTGCTGAGCGTACTGACCCTGTACTGAAAGCAGAACTGGGTTCACGCCT
AGACAGCCCACGTCTACGTATCACTGAAGCCGTATGTAAAGGTGCAGTAGACACCGCAGAGAAACTGGCAGCGCCACTGA
TCATCGTAGCAACTGAAGCGGGTAAATCTGCTCGTTCAGTTCGTAAGTACTTCCCAACGGCTAACATTATTGCAGTCACC
ACCAACAAGAAAACAGCAGCTCAGTTAGTGCTTTCTAAAGGTGTAACGCCAGTTGTGGTTGACGCGATTGACAACACTGA
CGCTTTCTACCACCTAGGTAAAGAGATTGCGCTGCAATCTGGCCTTGGCAAGAAAGGCGACATCGTAGTGATGGTTTCAG
GCGCACTGGTTGCTTCTGGCACAACGAACACTGCATCAGTACACGTACTGTAA

Upstream 100 bases:

>100_bases
GCTAGACTTGGCAATTGACGAAATAAAGTTGCTACCGCCGAGAACACTTCAAAACCACCACCAACTTCAAAATCATTTTG
TTTATCGGAGAAACTCTTCC

Downstream 100 bases:

>100_bases
TCGGATGAATGCGACGGCCTTGCCTCGCTGAATACGATATAAAAACGGAGCCCAATGGGCTCCGTTTTTTATTACCACCC
GAAAAACTCTTTGTGATGAG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-1 [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MKKTKIVCTIGPKTESVEKLTELVNAGMNVMRLNFSHGDYVEHGTRITNFRKVMEVTGKQLAILLDTKGPEIRTIKLENG
DDVDLVAGQEFTFTTDTKVVGNKERVAVTYSGFAKDLNVGNRILVDDGLIEMEVLATTDTEVKCKVLNNGALGENKGVNL
PGVSVNLPALSEKDKNDLKFGCEQGVDFVAASFIRKGSDVKEIREVLASHGGQNIQIISKIENQEGLDNFDEILELSDGI
MVARGDLGVEIPAEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVANAIMDGTDAVMLSGETAKGKY
PVEAVKIMAQIAERTDPVLKAELGSRLDSPRLRITEAVCKGAVDTAEKLAAPLIIVATEAGKSARSVRKYFPTANIIAVT
TNKKTAAQLVLSKGVTPVVVDAIDNTDAFYHLGKEIALQSGLGKKGDIVVMVSGALVASGTTNTASVHVL

Sequences:

>Translated_470_residues
MKKTKIVCTIGPKTESVEKLTELVNAGMNVMRLNFSHGDYVEHGTRITNFRKVMEVTGKQLAILLDTKGPEIRTIKLENG
DDVDLVAGQEFTFTTDTKVVGNKERVAVTYSGFAKDLNVGNRILVDDGLIEMEVLATTDTEVKCKVLNNGALGENKGVNL
PGVSVNLPALSEKDKNDLKFGCEQGVDFVAASFIRKGSDVKEIREVLASHGGQNIQIISKIENQEGLDNFDEILELSDGI
MVARGDLGVEIPAEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVANAIMDGTDAVMLSGETAKGKY
PVEAVKIMAQIAERTDPVLKAELGSRLDSPRLRITEAVCKGAVDTAEKLAAPLIIVATEAGKSARSVRKYFPTANIIAVT
TNKKTAAQLVLSKGVTPVVVDAIDNTDAFYHLGKEIALQSGLGKKGDIVVMVSGALVASGTTNTASVHVL
>Mature_470_residues
MKKTKIVCTIGPKTESVEKLTELVNAGMNVMRLNFSHGDYVEHGTRITNFRKVMEVTGKQLAILLDTKGPEIRTIKLENG
DDVDLVAGQEFTFTTDTKVVGNKERVAVTYSGFAKDLNVGNRILVDDGLIEMEVLATTDTEVKCKVLNNGALGENKGVNL
PGVSVNLPALSEKDKNDLKFGCEQGVDFVAASFIRKGSDVKEIREVLASHGGQNIQIISKIENQEGLDNFDEILELSDGI
MVARGDLGVEIPAEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVANAIMDGTDAVMLSGETAKGKY
PVEAVKIMAQIAERTDPVLKAELGSRLDSPRLRITEAVCKGAVDTAEKLAAPLIIVATEAGKSARSVRKYFPTANIIAVT
TNKKTAAQLVLSKGVTPVVVDAIDNTDAFYHLGKEIALQSGLGKKGDIVVMVSGALVASGTTNTASVHVL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=487, Percent_Identity=49.2813141683778, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI33286420, Length=487, Percent_Identity=49.2813141683778, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI33286418, Length=486, Percent_Identity=49.1769547325103, Blast_Score=417, Evalue=1e-117,
Organism=Homo sapiens, GI10835121, Length=488, Percent_Identity=48.5655737704918, Blast_Score=411, Evalue=1e-115,
Organism=Homo sapiens, GI32967597, Length=488, Percent_Identity=48.5655737704918, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=52.7777777777778, Blast_Score=270, Evalue=3e-72,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=52.7777777777778, Blast_Score=270, Evalue=3e-72,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=47.8813559322034, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=47.8813559322034, Blast_Score=197, Evalue=2e-50,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=46.9767441860465, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI1787965, Length=470, Percent_Identity=78.0851063829787, Blast_Score=734, Evalue=0.0,
Organism=Escherichia coli, GI1788160, Length=474, Percent_Identity=37.9746835443038, Blast_Score=286, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI17544584, Length=480, Percent_Identity=44.1666666666667, Blast_Score=372, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71984413, Length=482, Percent_Identity=46.0580912863071, Blast_Score=358, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI17506829, Length=485, Percent_Identity=45.7731958762887, Blast_Score=358, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI71984406, Length=485, Percent_Identity=45.7731958762887, Blast_Score=358, Evalue=3e-99,
Organism=Caenorhabditis elegans, GI17506831, Length=485, Percent_Identity=45.979381443299, Blast_Score=358, Evalue=4e-99,
Organism=Saccharomyces cerevisiae, GI6319279, Length=481, Percent_Identity=47.6091476091476, Blast_Score=379, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324923, Length=482, Percent_Identity=44.6058091286307, Blast_Score=366, Evalue=1e-102,
Organism=Drosophila melanogaster, GI28571814, Length=481, Percent_Identity=47.4012474012474, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24648964, Length=481, Percent_Identity=47.4012474012474, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24648966, Length=444, Percent_Identity=46.8468468468468, Blast_Score=350, Evalue=8e-97,
Organism=Drosophila melanogaster, GI24581235, Length=480, Percent_Identity=35.2083333333333, Blast_Score=274, Evalue=1e-73,
Organism=Drosophila melanogaster, GI24646914, Length=270, Percent_Identity=47.7777777777778, Blast_Score=218, Evalue=8e-57,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50440; Mature: 50440

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTKIVCTIGPKTESVEKLTELVNAGMNVMRLNFSHGDYVEHGTRITNFRKVMEVTGKQ
CCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHCCCHHHHHHHHHHHCCCE
LAILLDTKGPEIRTIKLENGDDVDLVAGQEFTFTTDTKVVGNKERVAVTYSGFAKDLNVG
EEEEEECCCCEEEEEEECCCCCEEEEECCEEEEECCCEEECCCCEEEEEECCCHHHCCCC
NRILVDDGLIEMEVLATTDTEVKCKVLNNGALGENKGVNLPGVSVNLPALSEKDKNDLKF
CEEEEECCCEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHH
GCEQGVDFVAASFIRKGSDVKEIREVLASHGGQNIQIISKIENQEGLDNFDEILELSDGI
HHHHCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHCCCE
MVARGDLGVEIPAEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVAN
EEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCCCHHHHHH
AIMDGTDAVMLSGETAKGKYPVEAVKIMAQIAERTDPVLKAELGSRLDSPRLRITEAVCK
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHC
GAVDTAEKLAAPLIIVATEAGKSARSVRKYFPTANIIAVTTNKKTAAQLVLSKGVTPVVV
CCHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCEEEE
DAIDNTDAFYHLGKEIALQSGLGKKGDIVVMVSGALVASGTTNTASVHVL
EECCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECEEEECCCCCCEEEEEC
>Mature Secondary Structure
MKKTKIVCTIGPKTESVEKLTELVNAGMNVMRLNFSHGDYVEHGTRITNFRKVMEVTGKQ
CCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHCCCHHHHHHHHHHHCCCE
LAILLDTKGPEIRTIKLENGDDVDLVAGQEFTFTTDTKVVGNKERVAVTYSGFAKDLNVG
EEEEEECCCCEEEEEEECCCCCEEEEECCEEEEECCCEEECCCCEEEEEECCCHHHCCCC
NRILVDDGLIEMEVLATTDTEVKCKVLNNGALGENKGVNLPGVSVNLPALSEKDKNDLKF
CEEEEECCCEEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHH
GCEQGVDFVAASFIRKGSDVKEIREVLASHGGQNIQIISKIENQEGLDNFDEILELSDGI
HHHHCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHCCCE
MVARGDLGVEIPAEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVAN
EEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCCCCCHHHHHH
AIMDGTDAVMLSGETAKGKYPVEAVKIMAQIAERTDPVLKAELGSRLDSPRLRITEAVCK
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHC
GAVDTAEKLAAPLIIVATEAGKSARSVRKYFPTANIIAVTTNKKTAAQLVLSKGVTPVVV
CCHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCEEEE
DAIDNTDAFYHLGKEIALQSGLGKKGDIVVMVSGALVASGTTNTASVHVL
EECCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECEEEECCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]