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The map label for this gene is gshB

Identifier: 227080678

GI number: 227080678

Start: 481667

End: 482623

Strand: Reverse

Name: gshB

Synonym: VCM66_0453

Alternate gene names: 227080678

Gene position: 482623-481667 (Counterclockwise)

Preceding gene: 227080679

Following gene: 227080677

Centisome position: 16.69

GC content: 49.32

Gene sequence:

>957_bases
ATGATTAAACTCGGTATTGTGATGGACCCTATCGCGTCCATTAACATCAAAAAAGATTCCAGCTTTGCCATGATGCTTGA
AGCGCAGCGTCGCGGTTATGAAATCCATTATATGGAGATGAATGATCTACACTTAGAGCAAGGTGTAGCACTGGCCGATA
CTAAAGTTGTCGAGCTGAAAGAAGATCCGAGCGGTTGGTACCAATTCAAATCTGAGCAAACCATCGCACTTTCTGAATTG
GATGCGATTTTAATGCGTAAGGATCCGCCGTTCGACACCGAATACATCTACGCAACCTACATTTTGGAGCGCGCGGAAGA
TGAAGGCGTATTAGTGGTGAACAAACCACAAAGCCTGCGCGATTGTAATGAAAAACTGTTCACCGCTTGGTTCCCAGAGC
TGACGCCGATCACTATGGTGACGCGCAAAGCCGAGAAGATTAAAGCCTTCCGTGAACAGCATGGGGATGTGATTCTAAAA
CCCCTCGACGGTATGGGCGGTGCTTCGATTTTCCGCGTTAAAGAGGGCGATCCAAACCTCTCAGTGATCATCGAAACCTT
AACCAACCACGGCCAGAATTACTGCATGGCGCAGACCTTTGTGCCAGATATCAGCAACGGTGATAAACGCATTTTGGTGG
TCGATGGTGAACCTATGCCTTACTGCTTGGCGCGTATCCCCGCCAAAGGGGAAACCCGCGGTAACTTGGCGGCTGGCGGT
CGTGGTGAGCCTCGCCCACTGAGTGAAACCGATAAGAAAATTGCCCTAGCGGTCGCACCGACTCTCAAAGAGAAAGGGCT
GCTGTTTGTAGGCTTAGATGTCATTGGCGATAAACTGACCGAAATTAACGTCACCAGCCCAACCTGCATTCGAGAGATTG
AAGCTGCGTACGATATTTCAATCACAGGCAAGCTGATGGATGCCATTGAGCGTCGCTTAAAAGCGACGGCGATGTGA

Upstream 100 bases:

>100_bases
TTGTTAGGCCCACGCGTACTACGCACAGAAACGGCGGCGCTCACGGCGATAACTGCACTACAAGTTCGTTTCGGCGATCT
TGGCTAAATGGAGATAAACC

Downstream 100 bases:

>100_bases
GCAACCATAGCAGTGACATAGAGGTTGGACACAGTATGAATCTTACCAATCATTTTCTGGTTGCCATGCCAAGCATGAAG
GATCCTTACTTCAAGCGCAG

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MIKLGIVMDPIASINIKKDSSFAMMLEAQRRGYEIHYMEMNDLHLEQGVALADTKVVELKEDPSGWYQFKSEQTIALSEL
DAILMRKDPPFDTEYIYATYILERAEDEGVLVVNKPQSLRDCNEKLFTAWFPELTPITMVTRKAEKIKAFREQHGDVILK
PLDGMGGASIFRVKEGDPNLSVIIETLTNHGQNYCMAQTFVPDISNGDKRILVVDGEPMPYCLARIPAKGETRGNLAAGG
RGEPRPLSETDKKIALAVAPTLKEKGLLFVGLDVIGDKLTEINVTSPTCIREIEAAYDISITGKLMDAIERRLKATAM

Sequences:

>Translated_318_residues
MIKLGIVMDPIASINIKKDSSFAMMLEAQRRGYEIHYMEMNDLHLEQGVALADTKVVELKEDPSGWYQFKSEQTIALSEL
DAILMRKDPPFDTEYIYATYILERAEDEGVLVVNKPQSLRDCNEKLFTAWFPELTPITMVTRKAEKIKAFREQHGDVILK
PLDGMGGASIFRVKEGDPNLSVIIETLTNHGQNYCMAQTFVPDISNGDKRILVVDGEPMPYCLARIPAKGETRGNLAAGG
RGEPRPLSETDKKIALAVAPTLKEKGLLFVGLDVIGDKLTEINVTSPTCIREIEAAYDISITGKLMDAIERRLKATAM
>Mature_318_residues
MIKLGIVMDPIASINIKKDSSFAMMLEAQRRGYEIHYMEMNDLHLEQGVALADTKVVELKEDPSGWYQFKSEQTIALSEL
DAILMRKDPPFDTEYIYATYILERAEDEGVLVVNKPQSLRDCNEKLFTAWFPELTPITMVTRKAEKIKAFREQHGDVILK
PLDGMGGASIFRVKEGDPNLSVIIETLTNHGQNYCMAQTFVPDISNGDKRILVVDGEPMPYCLARIPAKGETRGNLAAGG
RGEPRPLSETDKKIALAVAPTLKEKGLLFVGLDVIGDKLTEINVTSPTCIREIEAAYDISITGKLMDAIERRLKATAM

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1789316, Length=314, Percent_Identity=74.5222929936306, Blast_Score=498, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSHB_VIBCH (Q9KUP7)

Other databases:

- EMBL:   AE003852
- PIR:   A82319
- RefSeq:   NP_230122.1
- ProteinModelPortal:   Q9KUP7
- SMR:   Q9KUP7
- GeneID:   2615130
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0468
- TIGR:   VC_0468
- HOGENOM:   HBG624526
- OMA:   WMRKDPP
- ProtClustDB:   PRK05246
- BRENDA:   6.3.2.3
- HAMAP:   MF_00162
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01380

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.3

Molecular weight: Translated: 35408; Mature: 35408

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLGIVMDPIASINIKKDSSFAMMLEAQRRGYEIHYMEMNDLHLEQGVALADTKVVELK
CEEECEEECCCCEEEEECCCCEEEEEEHHHCCCEEEEEEECCEEHHCCCEEECEEEEEEE
EDPSGWYQFKSEQTIALSELDAILMRKDPPFDTEYIYATYILERAEDEGVLVVNKPQSLR
CCCCCCEEECCCCEEEHHHHHHHEECCCCCCCCCEEEEEEEEECCCCCCEEEEECCHHHH
DCNEKLFTAWFPELTPITMVTRKAEKIKAFREQHGDVILKPLDGMGGASIFRVKEGDPNL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCC
SVIIETLTNHGQNYCMAQTFVPDISNGDKRILVVDGEPMPYCLARIPAKGETRGNLAAGG
EEEEEEHHCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCHHHHCCCCCCCCCCCCCCCC
RGEPRPLSETDKKIALAVAPTLKEKGLLFVGLDVIGDKLTEINVTSPTCIREIEAAYDIS
CCCCCCCCCCCCEEEEEEECCCCCCCEEEEEHHHHCCCEEEEECCCCHHHHHHHHHEEEE
ITGKLMDAIERRLKATAM
EHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKLGIVMDPIASINIKKDSSFAMMLEAQRRGYEIHYMEMNDLHLEQGVALADTKVVELK
CEEECEEECCCCEEEEECCCCEEEEEEHHHCCCEEEEEEECCEEHHCCCEEECEEEEEEE
EDPSGWYQFKSEQTIALSELDAILMRKDPPFDTEYIYATYILERAEDEGVLVVNKPQSLR
CCCCCCEEECCCCEEEHHHHHHHEECCCCCCCCCEEEEEEEEECCCCCCEEEEECCHHHH
DCNEKLFTAWFPELTPITMVTRKAEKIKAFREQHGDVILKPLDGMGGASIFRVKEGDPNL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECCCCCC
SVIIETLTNHGQNYCMAQTFVPDISNGDKRILVVDGEPMPYCLARIPAKGETRGNLAAGG
EEEEEEHHCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCHHHHCCCCCCCCCCCCCCCC
RGEPRPLSETDKKIALAVAPTLKEKGLLFVGLDVIGDKLTEINVTSPTCIREIEAAYDIS
CCCCCCCCCCCCEEEEEEECCCCCCCEEEEEHHHHCCCEEEEECCCCHHHHHHHHHEEEE
ITGKLMDAIERRLKATAM
EHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301