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The map label for this gene is rsmE [H]

Identifier: 227080679

GI number: 227080679

Start: 482637

End: 483368

Strand: Reverse

Name: rsmE [H]

Synonym: VCM66_0454

Alternate gene names: 227080679

Gene position: 483368-482637 (Counterclockwise)

Preceding gene: 227080680

Following gene: 227080678

Centisome position: 16.71

GC content: 51.37

Gene sequence:

>732_bases
ATGCGCATCCCTCGAATTTATCATCCAGAACCGATCCACCAGCTTGGCACCGTTGCCCTGAGTGAAGATGCCGCAGGGCA
TATCGGTCGCGTATTGCGTATGCAAGCTGGGCAAGAAGTGCTGCTGTTTGATGGTTTGGGGGCTGAATTCCCAGCCGTGA
TCAGTGAAGTTGGCAAAAAACAGGTATTGGTCAATATCACTGAGCGCGTAGAAAACAGCATTGAATCGCCGCTCGATCTG
CATCTTGGGCAGGTGATTTCACGCGGCGATAAGATGGAATTCACCATCCAAAAATCGGTGGAATTGGGGGTAACTACCAT
CACCCCTCTGCTTTCTGAGCGTTGTGGTGTGAAGCTGGATGAAAAACGCTTCGAGAAGAAGATTCAGCAGTGGCAGAAAA
TTGCGATCAGCGCTTGTGAGCAGTGTGGCCGTAATACCGTGCCAGAAATCCGCCCAATTATGGATCTCGAAGCTTGGTGC
GCAGAGCCCACCGAGGCACTGAAACTCAATTTGCACCCACGCGCTAAATACTCGCTCAACACCTTACCCACCCCAGTGAG
CAAAGTTCGTTTGCTGATTGGTCCTGAAGGTGGTCTGTCAGCGGAAGAAATTGATATGACTCGCCGTTACCAGTTTGAAG
AAACCTTGTTAGGCCCACGCGTACTACGCACAGAAACGGCGGCGCTCACGGCGATAACTGCACTACAAGTTCGTTTCGGC
GATCTTGGCTAA

Upstream 100 bases:

>100_bases
CACTTGCATCCATGTTAGAGGCCAGATATCCCTCTTCCAACTGAAGTTACACTTTACGTTGGAAGACGGTAACCTCGTTT
AATGATTAGGAATTATCCGC

Downstream 100 bases:

>100_bases
ATGGAGATAAACCATGATTAAACTCGGTATTGTGATGGACCCTATCGCGTCCATTAACATCAAAAAAGATTCCAGCTTTG
CCATGATGCTTGAAGCGCAG

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MRIPRIYHPEPIHQLGTVALSEDAAGHIGRVLRMQAGQEVLLFDGLGAEFPAVISEVGKKQVLVNITERVENSIESPLDL
HLGQVISRGDKMEFTIQKSVELGVTTITPLLSERCGVKLDEKRFEKKIQQWQKIAISACEQCGRNTVPEIRPIMDLEAWC
AEPTEALKLNLHPRAKYSLNTLPTPVSKVRLLIGPEGGLSAEEIDMTRRYQFEETLLGPRVLRTETAALTAITALQVRFG
DLG

Sequences:

>Translated_243_residues
MRIPRIYHPEPIHQLGTVALSEDAAGHIGRVLRMQAGQEVLLFDGLGAEFPAVISEVGKKQVLVNITERVENSIESPLDL
HLGQVISRGDKMEFTIQKSVELGVTTITPLLSERCGVKLDEKRFEKKIQQWQKIAISACEQCGRNTVPEIRPIMDLEAWC
AEPTEALKLNLHPRAKYSLNTLPTPVSKVRLLIGPEGGLSAEEIDMTRRYQFEETLLGPRVLRTETAALTAITALQVRFG
DLG
>Mature_243_residues
MRIPRIYHPEPIHQLGTVALSEDAAGHIGRVLRMQAGQEVLLFDGLGAEFPAVISEVGKKQVLVNITERVENSIESPLDL
HLGQVISRGDKMEFTIQKSVELGVTTITPLLSERCGVKLDEKRFEKKIQQWQKIAISACEQCGRNTVPEIRPIMDLEAWC
AEPTEALKLNLHPRAKYSLNTLPTPVSKVRLLIGPEGGLSAEEIDMTRRYQFEETLLGPRVLRTETAALTAITALQVRFG
DLG

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=243, Percent_Identity=72.4279835390947, Blast_Score=358, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27013; Mature: 27013

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIPRIYHPEPIHQLGTVALSEDAAGHIGRVLRMQAGQEVLLFDGLGAEFPAVISEVGKK
CCCCCCCCCCHHHHHHHEHCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCH
QVLVNITERVENSIESPLDLHLGQVISRGDKMEFTIQKSVELGVTTITPLLSERCGVKLD
HEEEHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCHHCCHHHHHHHHHHHCCCCCC
EKRFEKKIQQWQKIAISACEQCGRNTVPEIRPIMDLEAWCAEPTEALKLNLHPRAKYSLN
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHEEEECCCCCCEECC
TLPTPVSKVRLLIGPEGGLSAEEIDMTRRYQFEETLLGPRVLRTETAALTAITALQVRFG
CCCCCHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
DLG
CCC
>Mature Secondary Structure
MRIPRIYHPEPIHQLGTVALSEDAAGHIGRVLRMQAGQEVLLFDGLGAEFPAVISEVGKK
CCCCCCCCCCHHHHHHHEHCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCH
QVLVNITERVENSIESPLDLHLGQVISRGDKMEFTIQKSVELGVTTITPLLSERCGVKLD
HEEEHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEECCHHCCHHHHHHHHHHHCCCCCC
EKRFEKKIQQWQKIAISACEQCGRNTVPEIRPIMDLEAWCAEPTEALKLNLHPRAKYSLN
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHEEEECCCCCCEECC
TLPTPVSKVRLLIGPEGGLSAEEIDMTRRYQFEETLLGPRVLRTETAALTAITALQVRFG
CCCCCHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
DLG
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]