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The map label for this gene is mutY [H]

Identifier: 227080662

GI number: 227080662

Start: 467953

End: 469014

Strand: Reverse

Name: mutY [H]

Synonym: VCM66_0437

Alternate gene names: 227080662

Gene position: 469014-467953 (Counterclockwise)

Preceding gene: 227080665

Following gene: 227080661

Centisome position: 16.21

GC content: 51.41

Gene sequence:

>1062_bases
GTGACTCCTTTTGCACAGGCCATCCTTACTTGGTATGACGCCTACGGCCGCAAAAACCTGCCGTGGCAACAAAATAAAAA
TGCGTATCGCGTTTGGTTATCGGAAATCATGTTACAGCAGACCCAAGTCGCGACCGTGATCCCCTACTTTGAACGCTTTT
TAGAGCGCTTCCCGACCGTACACGCCCTCGCGGCAGCGCCGCAAGATGAAGTGCTGCATTTCTGGACGGGGCTTGGCTAC
TACGCCAGAGCGCGCAATCTGCATAAAGCAGCGCAAATGGTTGTGAGTGAATATGGCGGCGAATTTCCCACCGATTTAGA
GCAGATGAATGCGCTACCCGGTGTTGGCCGTTCTACCGCAGCAGCCGTGCTCTCTTCTGTGTATAAAAAACCGCATGCGA
TTTTGGATGGCAACGTGAAGCGCACGTTAGCGCGCTGCTTTGCCGTTGAAGGTTGGCCGGGGCAAAAAAGTGTCGAAAAC
CAGCTTTGGCATTATGCAGAAATGCACACGCCCAAAGTGGATGTTGATAAATACAACCAAGCCATGATGGATATGGGCGC
GATGATCTGCACTCGCAGCAAGCCAAAATGCAGCCTGTGCCCAGTAGAATCGTTTTGCCTTGCCAAGCAGCAAGGCAACC
CCCAAGAGTATCCGGGCAAGAAACCGAAAACCGATAAACCCGTCAAAGCCACTTGGTTTGTCATGCTCTATCACGACAAT
GCCGTCTGGCTTGAGCAGCGCCCGCAAAGTGGAATTTGGGGCGGTTTGTACTGCTTCCCGCAATCAGAGATCGCCAATAT
TCAAACCACCATAGATCAGCGCGCCATCGGCGATAGCACAATAACATCGCAGAAAACCCTGATCGCATTTCGCCACACCT
TTAGCCACTACCATCTCGATATCACGCCGATTTTGCTGCAATTAAGCCGCAAACCGGACATCGTCATGGAAGGGAGCAAA
GGTCTTTGGTATAACTTAAGCCACCCCGATGAGATTGGTCTCGCGGCACCAGTGAAACAACTGTTGCACACCTTACCTTT
CGACATTGATAGCCACATTTAA

Upstream 100 bases:

>100_bases
GGAAGGAGGAGAATATCCCCAATGTTTGTCGGTTTTACTTCACCACCAATCTGTGGTGCAATTTTGCTCCACTAGTTTAG
ACAATAATAATGAGCAAGTC

Downstream 100 bases:

>100_bases
AGAGGAAGAGTGAATGGCTCGCACCGTATTTTGTACCCGATTGCAGAAAGAAGCCGATGGCTTAGATTTCCAACTCTATC
CCGGCGAACTGGGCAAACGC

Product: A/G-specific adenine glycosylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MTPFAQAILTWYDAYGRKNLPWQQNKNAYRVWLSEIMLQQTQVATVIPYFERFLERFPTVHALAAAPQDEVLHFWTGLGY
YARARNLHKAAQMVVSEYGGEFPTDLEQMNALPGVGRSTAAAVLSSVYKKPHAILDGNVKRTLARCFAVEGWPGQKSVEN
QLWHYAEMHTPKVDVDKYNQAMMDMGAMICTRSKPKCSLCPVESFCLAKQQGNPQEYPGKKPKTDKPVKATWFVMLYHDN
AVWLEQRPQSGIWGGLYCFPQSEIANIQTTIDQRAIGDSTITSQKTLIAFRHTFSHYHLDITPILLQLSRKPDIVMEGSK
GLWYNLSHPDEIGLAAPVKQLLHTLPFDIDSHI

Sequences:

>Translated_353_residues
MTPFAQAILTWYDAYGRKNLPWQQNKNAYRVWLSEIMLQQTQVATVIPYFERFLERFPTVHALAAAPQDEVLHFWTGLGY
YARARNLHKAAQMVVSEYGGEFPTDLEQMNALPGVGRSTAAAVLSSVYKKPHAILDGNVKRTLARCFAVEGWPGQKSVEN
QLWHYAEMHTPKVDVDKYNQAMMDMGAMICTRSKPKCSLCPVESFCLAKQQGNPQEYPGKKPKTDKPVKATWFVMLYHDN
AVWLEQRPQSGIWGGLYCFPQSEIANIQTTIDQRAIGDSTITSQKTLIAFRHTFSHYHLDITPILLQLSRKPDIVMEGSK
GLWYNLSHPDEIGLAAPVKQLLHTLPFDIDSHI
>Mature_352_residues
TPFAQAILTWYDAYGRKNLPWQQNKNAYRVWLSEIMLQQTQVATVIPYFERFLERFPTVHALAAAPQDEVLHFWTGLGYY
ARARNLHKAAQMVVSEYGGEFPTDLEQMNALPGVGRSTAAAVLSSVYKKPHAILDGNVKRTLARCFAVEGWPGQKSVENQ
LWHYAEMHTPKVDVDKYNQAMMDMGAMICTRSKPKCSLCPVESFCLAKQQGNPQEYPGKKPKTDKPVKATWFVMLYHDNA
VWLEQRPQSGIWGGLYCFPQSEIANIQTTIDQRAIGDSTITSQKTLIAFRHTFSHYHLDITPILLQLSRKPDIVMEGSKG
LWYNLSHPDEIGLAAPVKQLLHTLPFDIDSHI

Specific function: Adenine glycosylase active on G-A mispairs. MutY also corrects error-prone DNA synthesis past GO lesions which are due to the oxidatively damaged form of guanine:7,8-dihydro-8- oxoguanine (8-oxo-dGTP) [H]

COG id: COG1194

COG function: function code L; A/G-specific DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI6912520, Length=360, Percent_Identity=34.1666666666667, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI190358497, Length=360, Percent_Identity=34.1666666666667, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI115298650, Length=360, Percent_Identity=34.1666666666667, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI115298648, Length=360, Percent_Identity=34.1666666666667, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI115298654, Length=360, Percent_Identity=34.1666666666667, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI115298652, Length=360, Percent_Identity=34.1666666666667, Blast_Score=192, Evalue=4e-49,
Organism=Escherichia coli, GI1789331, Length=343, Percent_Identity=60.0583090379009, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI1787920, Length=132, Percent_Identity=29.5454545454545, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170
- InterPro:   IPR005760
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: 3.2.2.-

Molecular weight: Translated: 40083; Mature: 39952

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPFAQAILTWYDAYGRKNLPWQQNKNAYRVWLSEIMLQQTQVATVIPYFERFLERFPTV
CCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
HALAAAPQDEVLHFWTGLGYYARARNLHKAAQMVVSEYGGEFPTDLEQMNALPGVGRSTA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHH
AAVLSSVYKKPHAILDGNVKRTLARCFAVEGWPGQKSVENQLWHYAEMHTPKVDVDKYNQ
HHHHHHHHHCCCEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
AMMDMGAMICTRSKPKCSLCPVESFCLAKQQGNPQEYPGKKPKTDKPVKATWFVMLYHDN
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEEECC
AVWLEQRPQSGIWGGLYCFPQSEIANIQTTIDQRAIGDSTITSQKTLIAFRHTFSHYHLD
EEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHEEEE
ITPILLQLSRKPDIVMEGSKGLWYNLSHPDEIGLAAPVKQLLHTLPFDIDSHI
HHHHHHHHCCCCCEEEECCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TPFAQAILTWYDAYGRKNLPWQQNKNAYRVWLSEIMLQQTQVATVIPYFERFLERFPTV
CHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
HALAAAPQDEVLHFWTGLGYYARARNLHKAAQMVVSEYGGEFPTDLEQMNALPGVGRSTA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHH
AAVLSSVYKKPHAILDGNVKRTLARCFAVEGWPGQKSVENQLWHYAEMHTPKVDVDKYNQ
HHHHHHHHHCCCEEECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
AMMDMGAMICTRSKPKCSLCPVESFCLAKQQGNPQEYPGKKPKTDKPVKATWFVMLYHDN
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEEECC
AVWLEQRPQSGIWGGLYCFPQSEIANIQTTIDQRAIGDSTITSQKTLIAFRHTFSHYHLD
EEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHHHHEEEE
ITPILLQLSRKPDIVMEGSKGLWYNLSHPDEIGLAAPVKQLLHTLPFDIDSHI
HHHHHHHHCCCCCEEEECCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 4Fe-4S Cluster [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Hydrolysing N-glycosyl compounds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2197596; 2001994; 9278503; 9846876 [H]