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The map label for this gene is mltC [H]

Identifier: 227080660

GI number: 227080660

Start: 466438

End: 467628

Strand: Reverse

Name: mltC [H]

Synonym: VCM66_0435

Alternate gene names: 227080660

Gene position: 467628-466438 (Counterclockwise)

Preceding gene: 227080661

Following gene: 227080659

Centisome position: 16.17

GC content: 46.6

Gene sequence:

>1191_bases
ATGCCGCTGGGTTATGATGTCATTTTCGTATCAGGAGTAGCGAAAGCTCGCTTTAGGAGGCGCATGAGAAAGTTAGTGTT
ATGTATCACCGCTCTATTATTGAGTGGCTGTAGTCGTGAATTTATCGAGAAAATTTACGATGTTGATTACGAGCCCACTA
ACCGTTTCGCCAAGAACTTAGCCGAATTACCTGGACAGTTTCAGAAAGACACCGCCGCACTGGATGCATTAATCAACAGC
TTCTCCGGCAATATCGAAAAACGTTGGGGACGCCGCGAGCTAAAAATCGCCGGAAAAAATAACTACGTCAAATACATAGA
CAACTACCTTAGCCGCTCTGAAGTCAACTTCACCGAAGGTCGAATCATAGTAGAAACTGTTTCACCTATCGATCCTAAAG
CCCATTTACGCAATGCGATCATCACCACCTTACTGACTCCCGATGATCCCGCGCACGTCGATCTTTTCTCCTCAAAAGAC
ATTGAACTCAAAGGCCAACCCTTTTTGTATCAGCAGGTACTCGATCAAGATGGTCAGCCGATCCAGTGGTCATGGCGCGC
TAACCGTTACGCCGATTACCTCATCGCTAACCATTTGAAAGTGAAGCAAGTCGATTTCAAAAAAGCCTATTACGTCGAAA
TCCCCATGGTCAAAGATCAAATCGATATCCGTGGCTACAAATACGCCAGTATCGTGCGTAAAGCTTCTCGCAAATACGAC
ATCCCGGAAGATCTGATCTACGCGATCATCAAAACCGAAAGCAGTTTCAACCCCTATGCGGTGAGTTGGGCCAACGCCTA
TGGTTTAATGCAAGTGGTGCCTAAAACCGCAGGACGGGATGTGTTCAAGCTGGTCAAAAACCGCTCTGGCGAGCCCAGCC
CCGAGTATCTGTTTAACCCAGAAAACAACATTGATACGGGTACGGCCTATTTTTACATCCTGAAAAATCGCTATTTGAAA
GAGGTTCGCCACCCCACATCTCTCGAATACAGCATGATTTCTGCCTATAACGGCGGCACTGGCGGAGTGTTGAGTACCTT
TAGCAGTGATCGTCAACGCGCCATGCGTGACCTTAATGCCCTGCAACCTAACCAAGTGTATTGGGCGTTAACCAAAAAAC
ATCCGAATGCGGAAGCGCGTCGTTATCTGGAAAAAGTGACCAAATTTAAGAAGGAATTTAACGCGGGATAA

Upstream 100 bases:

>100_bases
TGAACTTCCTGTTTGAAGGTAAAGAAGTCCATATCGAAGGTTATACACCGCCTGCAAAGTAGGAAATTTCGGATTTCATC
TGCTTGCAAAAAATGACATC

Downstream 100 bases:

>100_bases
CAAAAAGCCCGCAGTTTGCTGAAAATAACGGCAATCAAACTGATTTTTCGAAATATTTCAAAAAACAGGTTGACGGGGAA
AGGGAAAATCCGTTTAATAG

Product: membrane-bound lytic murein transglycosylase C

Products: N-Acetylmuramic Acid Residues; N-Acetylglucosamine Residues [C]

Alternate protein names: Murein hydrolase C [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MPLGYDVIFVSGVAKARFRRRMRKLVLCITALLLSGCSREFIEKIYDVDYEPTNRFAKNLAELPGQFQKDTAALDALINS
FSGNIEKRWGRRELKIAGKNNYVKYIDNYLSRSEVNFTEGRIIVETVSPIDPKAHLRNAIITTLLTPDDPAHVDLFSSKD
IELKGQPFLYQQVLDQDGQPIQWSWRANRYADYLIANHLKVKQVDFKKAYYVEIPMVKDQIDIRGYKYASIVRKASRKYD
IPEDLIYAIIKTESSFNPYAVSWANAYGLMQVVPKTAGRDVFKLVKNRSGEPSPEYLFNPENNIDTGTAYFYILKNRYLK
EVRHPTSLEYSMISAYNGGTGGVLSTFSSDRQRAMRDLNALQPNQVYWALTKKHPNAEARRYLEKVTKFKKEFNAG

Sequences:

>Translated_396_residues
MPLGYDVIFVSGVAKARFRRRMRKLVLCITALLLSGCSREFIEKIYDVDYEPTNRFAKNLAELPGQFQKDTAALDALINS
FSGNIEKRWGRRELKIAGKNNYVKYIDNYLSRSEVNFTEGRIIVETVSPIDPKAHLRNAIITTLLTPDDPAHVDLFSSKD
IELKGQPFLYQQVLDQDGQPIQWSWRANRYADYLIANHLKVKQVDFKKAYYVEIPMVKDQIDIRGYKYASIVRKASRKYD
IPEDLIYAIIKTESSFNPYAVSWANAYGLMQVVPKTAGRDVFKLVKNRSGEPSPEYLFNPENNIDTGTAYFYILKNRYLK
EVRHPTSLEYSMISAYNGGTGGVLSTFSSDRQRAMRDLNALQPNQVYWALTKKHPNAEARRYLEKVTKFKKEFNAG
>Mature_395_residues
PLGYDVIFVSGVAKARFRRRMRKLVLCITALLLSGCSREFIEKIYDVDYEPTNRFAKNLAELPGQFQKDTAALDALINSF
SGNIEKRWGRRELKIAGKNNYVKYIDNYLSRSEVNFTEGRIIVETVSPIDPKAHLRNAIITTLLTPDDPAHVDLFSSKDI
ELKGQPFLYQQVLDQDGQPIQWSWRANRYADYLIANHLKVKQVDFKKAYYVEIPMVKDQIDIRGYKYASIVRKASRKYDI
PEDLIYAIIKTESSFNPYAVSWANAYGLMQVVPKTAGRDVFKLVKNRSGEPSPEYLFNPENNIDTGTAYFYILKNRYLKE
VRHPTSLEYSMISAYNGGTGGVLSTFSSDRQRAMRDLNALQPNQVYWALTKKHPNAEARRYLEKVTKFKKEFNAG

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082191, Length=326, Percent_Identity=51.5337423312883, Blast_Score=340, Evalue=9e-95,
Organism=Escherichia coli, GI87081855, Length=165, Percent_Identity=39.3939393939394, Blast_Score=122, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 45572; Mature: 45440

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLGYDVIFVSGVAKARFRRRMRKLVLCITALLLSGCSREFIEKIYDVDYEPTNRFAKNL
CCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHH
AELPGQFQKDTAALDALINSFSGNIEKRWGRRELKIAGKNNYVKYIDNYLSRSEVNFTEG
HHCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCC
RIIVETVSPIDPKAHLRNAIITTLLTPDDPAHVDLFSSKDIELKGQPFLYQQVLDQDGQP
EEEEEECCCCCHHHHHHHHHHHEEECCCCCCEEEEECCCCCEECCCCHHHHHHHCCCCCC
IQWSWRANRYADYLIANHLKVKQVDFKKAYYVEIPMVKDQIDIRGYKYASIVRKASRKYD
EEEEECCCHHHHHHHHCCCEEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCC
IPEDLIYAIIKTESSFNPYAVSWANAYGLMQVVPKTAGRDVFKLVKNRSGEPSPEYLFNP
CCHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEECC
ENNIDTGTAYFYILKNRYLKEVRHPTSLEYSMISAYNGGTGGVLSTFSSDRQRAMRDLNA
CCCCCCCCEEEEHHHHHHHHHHCCCCCCCCHHHEECCCCCCCHHHHHHHHHHHHHHHHHC
LQPNQVYWALTKKHPNAEARRYLEKVTKFKKEFNAG
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PLGYDVIFVSGVAKARFRRRMRKLVLCITALLLSGCSREFIEKIYDVDYEPTNRFAKNL
CCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHH
AELPGQFQKDTAALDALINSFSGNIEKRWGRRELKIAGKNNYVKYIDNYLSRSEVNFTEG
HHCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCC
RIIVETVSPIDPKAHLRNAIITTLLTPDDPAHVDLFSSKDIELKGQPFLYQQVLDQDGQP
EEEEEECCCCCHHHHHHHHHHHEEECCCCCCEEEEECCCCCEECCCCHHHHHHHCCCCCC
IQWSWRANRYADYLIANHLKVKQVDFKKAYYVEIPMVKDQIDIRGYKYASIVRKASRKYD
EEEEECCCHHHHHHHHCCCEEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCC
IPEDLIYAIIKTESSFNPYAVSWANAYGLMQVVPKTAGRDVFKLVKNRSGEPSPEYLFNP
CCHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEECC
ENNIDTGTAYFYILKNRYLKEVRHPTSLEYSMISAYNGGTGGVLSTFSSDRQRAMRDLNA
CCCCCCCCEEEEHHHHHHHHHHCCCCCCCCHHHEECCCCCCCHHHHHHHHHHHHHHHHHC
LQPNQVYWALTKKHPNAEARRYLEKVTKFKKEFNAG
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]