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The map label for this gene is ispB [H]

Identifier: 227080645

GI number: 227080645

Start: 449934

End: 450980

Strand: Reverse

Name: ispB [H]

Synonym: VCM66_0419

Alternate gene names: 227080645

Gene position: 450980-449934 (Counterclockwise)

Preceding gene: 227080652

Following gene: 227080642

Centisome position: 15.59

GC content: 46.9

Gene sequence:

>1047_bases
ATGATTTTTAGTGTATTATGCAGCGATTCCAGAAGACTAAATGCCTTACACGCTCTTATTTCAGCCGGATTTACGATGGA
TTTTAAAGCTATCCAAGCGCTTACTGCCAATGACATGGCAAAAGTGAACGAAACGATTCACGCTCAACTCAATTCTGACG
TTTCCCTAATCAACCAACTTGGTTTCTATATCATCAGTGGTGGTGGGAAGCGTTTACGCCCATTGCTGGCGGTTTTATCC
GCCAAAGCGCTCGGTTATCAAGGGTCACATCACACGACTGCTGCTGCGTTTATTGAATTCATTCATACGGCAACCCTGTT
GCACGATGATGTGGTCGATGAATCAGACTTGCGCCGCGGTAAAGCAACCGCCAATGCCGCGTTTGGTAATGCCGCCAGCG
TATTAGTCGGTGATTTTATCTACACTCGCTCGTTTCAGATGATGACAGAATTAGGATCAATGAAGATCCTGAAACTGATG
AGTGATGCAGTAAACGTGATCGCTGAAGGTGAAGTACAGCAATTAATGAACTGTAACGATCCTGATACCACCGAAGCCAG
CTACATGCAGGTTATTTACTCCAAAACTGCACGTCTGTTTGAAGCGGCAACCCAGATTGGCGCTATCCTGTGTGATGCTT
CTCCTGAAGTCGAACTGGCGATGCAAAACTACGGTAAATACCTAGGCACAGCCTTCCAACTGATTGATGATGTGATGGAT
TACACTTCAGATGGTGATGAGATGGGTAAAAACGTCGGTGACGATCTGGCAGAAGGCAAACCTACCCTACCACTGCTTTA
TGCGATGCAGCACGGGAATCCTGAGCAAGCCGCGATGATCCGTGAAGCCATCGAGAAAGCCAATGGCATGTCGCGTCTTA
ATGAGATTCTCGCAGCGATGGAGCAAACCGGTTCTTTACAATACACCATTGAAAAGGCGCTGCATGAAGCGGATAAAGCC
ATCGCTGAACTTGCGATTTTGCCAGAAAGCGAATACAAACAAGCCTTGATCACGCTCGCCCATATGGCGGTCAATCGCAG
TAAATAG

Upstream 100 bases:

>100_bases
AATGATGTGCCTATGACAAGCCATATTTTGAAAAAAATGGCGAAAAGCTAATCGCCAAACAAAAGTGCCGCAATCATGCC
TTTAAGCCATGTATTAATCA

Downstream 100 bases:

>100_bases
TCTGCAAATTCGGTGAGATGACCACAGCAACTCACTCGAGAATGGATTACGTTTTCTTACTAAAAAACCCCTCAGCTCTA
CGAGCTTGAGGGGTTTCTTT

Product: octaprenyl-diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MIFSVLCSDSRRLNALHALISAGFTMDFKAIQALTANDMAKVNETIHAQLNSDVSLINQLGFYIISGGGKRLRPLLAVLS
AKALGYQGSHHTTAAAFIEFIHTATLLHDDVVDESDLRRGKATANAAFGNAASVLVGDFIYTRSFQMMTELGSMKILKLM
SDAVNVIAEGEVQQLMNCNDPDTTEASYMQVIYSKTARLFEAATQIGAILCDASPEVELAMQNYGKYLGTAFQLIDDVMD
YTSDGDEMGKNVGDDLAEGKPTLPLLYAMQHGNPEQAAMIREAIEKANGMSRLNEILAAMEQTGSLQYTIEKALHEADKA
IAELAILPESEYKQALITLAHMAVNRSK

Sequences:

>Translated_348_residues
MIFSVLCSDSRRLNALHALISAGFTMDFKAIQALTANDMAKVNETIHAQLNSDVSLINQLGFYIISGGGKRLRPLLAVLS
AKALGYQGSHHTTAAAFIEFIHTATLLHDDVVDESDLRRGKATANAAFGNAASVLVGDFIYTRSFQMMTELGSMKILKLM
SDAVNVIAEGEVQQLMNCNDPDTTEASYMQVIYSKTARLFEAATQIGAILCDASPEVELAMQNYGKYLGTAFQLIDDVMD
YTSDGDEMGKNVGDDLAEGKPTLPLLYAMQHGNPEQAAMIREAIEKANGMSRLNEILAAMEQTGSLQYTIEKALHEADKA
IAELAILPESEYKQALITLAHMAVNRSK
>Mature_348_residues
MIFSVLCSDSRRLNALHALISAGFTMDFKAIQALTANDMAKVNETIHAQLNSDVSLINQLGFYIISGGGKRLRPLLAVLS
AKALGYQGSHHTTAAAFIEFIHTATLLHDDVVDESDLRRGKATANAAFGNAASVLVGDFIYTRSFQMMTELGSMKILKLM
SDAVNVIAEGEVQQLMNCNDPDTTEASYMQVIYSKTARLFEAATQIGAILCDASPEVELAMQNYGKYLGTAFQLIDDVMD
YTSDGDEMGKNVGDDLAEGKPTLPLLYAMQHGNPEQAAMIREAIEKANGMSRLNEILAAMEQTGSLQYTIEKALHEADKA
IAELAILPESEYKQALITLAHMAVNRSK

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=332, Percent_Identity=31.9277108433735, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI4758430, Length=248, Percent_Identity=23.3870967741935, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI83700220, Length=248, Percent_Identity=23.3870967741935, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1789578, Length=323, Percent_Identity=68.7306501547988, Blast_Score=470, Evalue=1e-134,
Organism=Escherichia coli, GI1786623, Length=238, Percent_Identity=30.2521008403361, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17505681, Length=328, Percent_Identity=31.7073170731707, Blast_Score=146, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6319475, Length=307, Percent_Identity=32.8990228013029, Blast_Score=136, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6325188, Length=247, Percent_Identity=22.6720647773279, Blast_Score=73, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24651612, Length=353, Percent_Identity=31.1614730878187, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI281365769, Length=283, Percent_Identity=24.0282685512367, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24660002, Length=283, Percent_Identity=24.0282685512367, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 37811; Mature: 37811

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFSVLCSDSRRLNALHALISAGFTMDFKAIQALTANDMAKVNETIHAQLNSDVSLINQL
CEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
GFYIISGGGKRLRPLLAVLSAKALGYQGSHHTTAAAFIEFIHTATLLHDDVVDESDLRRG
CEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCC
KATANAAFGNAASVLVGDFIYTRSFQMMTELGSMKILKLMSDAVNVIAEGEVQQLMNCND
CCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCC
PDTTEASYMQVIYSKTARLFEAATQIGAILCDASPEVELAMQNYGKYLGTAFQLIDDVMD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YTSDGDEMGKNVGDDLAEGKPTLPLLYAMQHGNPEQAAMIREAIEKANGMSRLNEILAAM
HCCCHHHHHHHCCCHHHCCCCCCHHHEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHH
EQTGSLQYTIEKALHEADKAIAELAILPESEYKQALITLAHMAVNRSK
HHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIFSVLCSDSRRLNALHALISAGFTMDFKAIQALTANDMAKVNETIHAQLNSDVSLINQL
CEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
GFYIISGGGKRLRPLLAVLSAKALGYQGSHHTTAAAFIEFIHTATLLHDDVVDESDLRRG
CEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCC
KATANAAFGNAASVLVGDFIYTRSFQMMTELGSMKILKLMSDAVNVIAEGEVQQLMNCND
CCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCC
PDTTEASYMQVIYSKTARLFEAATQIGAILCDASPEVELAMQNYGKYLGTAFQLIDDVMD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YTSDGDEMGKNVGDDLAEGKPTLPLLYAMQHGNPEQAAMIREAIEKANGMSRLNEILAAM
HCCCHHHHHHHCCCHHHCCCCCCHHHEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHH
EQTGSLQYTIEKALHEADKAIAELAILPESEYKQALITLAHMAVNRSK
HHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]