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The map label for this gene is hupA

Identifier: 227080487

GI number: 227080487

Start: 263159

End: 263431

Strand: Direct

Name: hupA

Synonym: VCM66_0258

Alternate gene names: 227080487

Gene position: 263159-263431 (Clockwise)

Preceding gene: 227080485

Following gene: 227080488

Centisome position: 9.1

GC content: 47.25

Gene sequence:

>273_bases
ATGAACAAGACCCAATTGATCGACTTTATCGCAGAGAAAGCGGACCTGACTAAAGTGCAAGCGAAAGCAGCTCTTGAAGC
GACTCTGGGTGCGGTTGAAGGTGCTCTGAAAGACGGCGACCAAGTACAACTGATTGGTTTTGGTACATTCAAAGTTAATC
ATCGTTCTGCTCGTACTGGCCGTAATCCAAAGACCGGTGAAGAGATCAAAATTGCGGCAGCTAACGTTCCTGCATTCGTA
GCAGGTAAAGCGCTGAAAGACGCGATCAAGTAA

Upstream 100 bases:

>100_bases
CGTCATAAAAGCGAAAGTTGCGCGACAAACCTTTGCCAGAGCGGGCATTTATGTTTTAGAGTGAGCTCGATTCGATAACC
TATTGAGAAGGGAAACCTAA

Downstream 100 bases:

>100_bases
TTGCTTTATACTACGCCGAGATTGAACATCTCGGCGTTTTTGTTTATGAAGCAGCAGTTATCCTTCCTCTTTTTGCTCTC
TGGCTTATTGGCCGGGTGCT

Product: DNA-binding protein HU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 90; Mature: 90

Protein sequence:

>90_residues
MNKTQLIDFIAEKADLTKVQAKAALEATLGAVEGALKDGDQVQLIGFGTFKVNHRSARTGRNPKTGEEIKIAAANVPAFV
AGKALKDAIK

Sequences:

>Translated_90_residues
MNKTQLIDFIAEKADLTKVQAKAALEATLGAVEGALKDGDQVQLIGFGTFKVNHRSARTGRNPKTGEEIKIAAANVPAFV
AGKALKDAIK
>Mature_90_residues
MNKTQLIDFIAEKADLTKVQAKAALEATLGAVEGALKDGDQVQLIGFGTFKVNHRSARTGRNPKTGEEIKIAAANVPAFV
AGKALKDAIK

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family

Homologues:

Organism=Escherichia coli, GI1790433, Length=90, Percent_Identity=78.8888888888889, Blast_Score=149, Evalue=4e-38,
Organism=Escherichia coli, GI1786644, Length=89, Percent_Identity=62.9213483146067, Blast_Score=111, Evalue=7e-27,
Organism=Escherichia coli, GI1788005, Length=90, Percent_Identity=37.7777777777778, Blast_Score=70, Evalue=2e-14,
Organism=Escherichia coli, GI1787141, Length=88, Percent_Identity=34.0909090909091, Blast_Score=68, Evalue=1e-13,

Paralogues:

None

Copy number: 1380 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): DBHA_VIBCH (Q9KV83)

Other databases:

- EMBL:   AE003852
- PIR:   H82343
- RefSeq:   NP_229929.1
- ProteinModelPortal:   Q9KV83
- SMR:   Q9KV83
- GeneID:   2614479
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0273
- TIGR:   VC_0273
- HOGENOM:   HBG705085
- OMA:   NAVMESV
- ProtClustDB:   CLSK793557
- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992
- Gene3D:   G3DSA:4.10.520.10
- PRINTS:   PR01727
- SMART:   SM00411

Pfam domain/function: PF00216 Bac_DNA_binding; SSF47729 IHF_like_DNA_bnd

EC number: NA

Molecular weight: Translated: 9491; Mature: 9491

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTQLIDFIAEKADLTKVQAKAALEATLGAVEGALKDGDQVQLIGFGTFKVNHRSARTG
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCCCC
RNPKTGEEIKIAAANVPAFVAGKALKDAIK
CCCCCCCEEEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNKTQLIDFIAEKADLTKVQAKAALEATLGAVEGALKDGDQVQLIGFGTFKVNHRSARTG
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCCCC
RNPKTGEEIKIAAANVPAFVAGKALKDAIK
CCCCCCCEEEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301