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The map label for this gene is mak [H]

Identifier: 227080485

GI number: 227080485

Start: 260401

End: 261381

Strand: Direct

Name: mak [H]

Synonym: VCM66_0255

Alternate gene names: 227080485

Gene position: 260401-261381 (Clockwise)

Preceding gene: 227080484

Following gene: 227080487

Centisome position: 9.0

GC content: 44.24

Gene sequence:

>981_bases
GTGCAGCAGGACGTGTCGCGCGAGCATACAATTAATAATGGTTCGAGCATGGCGGACCATGATGAGCATCCCATAGAGCA
AAAGGAATTCTACATGCTGATTGGATTGGATATTGGTGGCACAAAAATAGAAATATGTGTGTTAGATAAGCAAGGTAGTA
TGCTCTATCGACAGCGTATCGCAACGCCAGATAATTATTCTCAGTTTGTCGATTGTGTCTGTAGTCTGATTGTGGATGCT
GAGCAAGCCACCCAACCTGTTGATTCGATTGGTATCGGCCTACCTGGTGCAGTCAGTCCAGTTACTGGCCTGATCAAAAA
TGCCAATTGTACCTTCCTCAATGGGCAGGACTTATCAAGTGACTTGCAGTACCGTTTAGGTCGCGAGGTCAAACTAGCTA
ACGATGCAAACTGTTTTGCTCTTTCAGAAGCAATCGATGGGGCTGGTAAAGAAAGTATGGTCGTTTTTGGTGCGATACTC
GGTACAGGTTGCGGGGGTAGCATTGTAGTTAATCGTCAGGTATTAGTTGGGCCAAATGCCATATGTGGTGAATGGGGGCA
TAATCCACTCCCCGGATATCATCTCGAACAGGATGGGGCAGCACGGTACTGTTATTGTGGTCGACAGAATTGTATTGAGA
GATTTATTTCAGGAAGTGGTTTTCAGGATAGCTACCAAGCTTTAACGGGTGAATGTATTACCGCTTCAGAAATCATGAAG
CGGTATAAGCAACAAGAACCGGAAGCTATACATTGTTATACACAACTAATTGATCACATGGCACGTAGTTTTGCTGGATT
AGTTAATGTGCTCGATCCAGATATAATTGTTCTTGGTGGCGGACTTTCTAATATTGATGAGTTGTATCGAGATTTACCCA
CTGCAACTGCAAGACATGTGTTTTCTGACTCAGCTCAAGTACATTTTGCAAAAGCAGTATTTGGAGACAGCAGTGGCATT
CGTGGTGCAGCTTGGCTTTAG

Upstream 100 bases:

>100_bases
GATTGCGATGCTACTTTAGTTAGTCCTTGCGGCACCGTTCTTACAGTACACAAAGGGCAATCGGTCTTTATTCCCGCCTA
TACGGAAAAATACTGCTTAA

Downstream 100 bases:

>100_bases
CACTGAATAATTCGCAAACGCCCTGTTGATTAATGGGGCATTTACTGAGCAATAATCCCTCAAGCTTATTTCTGCTATTT
TGCTTCTTTGTCGACGCCAC

Product: ROK family protein

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MQQDVSREHTINNGSSMADHDEHPIEQKEFYMLIGLDIGGTKIEICVLDKQGSMLYRQRIATPDNYSQFVDCVCSLIVDA
EQATQPVDSIGIGLPGAVSPVTGLIKNANCTFLNGQDLSSDLQYRLGREVKLANDANCFALSEAIDGAGKESMVVFGAIL
GTGCGGSIVVNRQVLVGPNAICGEWGHNPLPGYHLEQDGAARYCYCGRQNCIERFISGSGFQDSYQALTGECITASEIMK
RYKQQEPEAIHCYTQLIDHMARSFAGLVNVLDPDIIVLGGGLSNIDELYRDLPTATARHVFSDSAQVHFAKAVFGDSSGI
RGAAWL

Sequences:

>Translated_326_residues
MQQDVSREHTINNGSSMADHDEHPIEQKEFYMLIGLDIGGTKIEICVLDKQGSMLYRQRIATPDNYSQFVDCVCSLIVDA
EQATQPVDSIGIGLPGAVSPVTGLIKNANCTFLNGQDLSSDLQYRLGREVKLANDANCFALSEAIDGAGKESMVVFGAIL
GTGCGGSIVVNRQVLVGPNAICGEWGHNPLPGYHLEQDGAARYCYCGRQNCIERFISGSGFQDSYQALTGECITASEIMK
RYKQQEPEAIHCYTQLIDHMARSFAGLVNVLDPDIIVLGGGLSNIDELYRDLPTATARHVFSDSAQVHFAKAVFGDSSGI
RGAAWL
>Mature_326_residues
MQQDVSREHTINNGSSMADHDEHPIEQKEFYMLIGLDIGGTKIEICVLDKQGSMLYRQRIATPDNYSQFVDCVCSLIVDA
EQATQPVDSIGIGLPGAVSPVTGLIKNANCTFLNGQDLSSDLQYRLGREVKLANDANCFALSEAIDGAGKESMVVFGAIL
GTGCGGSIVVNRQVLVGPNAICGEWGHNPLPGYHLEQDGAARYCYCGRQNCIERFISGSGFQDSYQALTGECITASEIMK
RYKQQEPEAIHCYTQLIDHMARSFAGLVNVLDPDIIVLGGGLSNIDELYRDLPTATARHVFSDSAQVHFAKAVFGDSSGI
RGAAWL

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Homo sapiens, GI298566315, Length=272, Percent_Identity=24.6323529411765, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI190014632, Length=272, Percent_Identity=24.6323529411765, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI298566325, Length=272, Percent_Identity=24.6323529411765, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4885285, Length=272, Percent_Identity=24.6323529411765, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI87081733, Length=301, Percent_Identity=47.8405315614618, Blast_Score=290, Evalue=6e-80,
Organism=Escherichia coli, GI1787363, Length=299, Percent_Identity=38.7959866220736, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1790522, Length=320, Percent_Identity=24.0625, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786891, Length=280, Percent_Identity=21.7857142857143, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 35290; Mature: 35290

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQDVSREHTINNGSSMADHDEHPIEQKEFYMLIGLDIGGTKIEICVLDKQGSMLYRQRI
CCCCCCHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEEEECCCCCHHHHHHC
ATPDNYSQFVDCVCSLIVDAEQATQPVDSIGIGLPGAVSPVTGLIKNANCTFLNGQDLSS
CCCCCHHHHHHHHHHHHHCCHHHHCCHHHHCCCCCCCHHHHHHHHHCCCEEEECCCCCCH
DLQYRLGREVKLANDANCFALSEAIDGAGKESMVVFGAILGTGCGGSIVVNRQVLVGPNA
HHHHHCCCEEEECCCCCCEEHHHHHCCCCCCCEEEEEHHHHCCCCCEEEEECEEEECCCH
ICGEWGHNPLPGYHLEQDGAARYCYCGRQNCIERFISGSGFQDSYQALTGECITASEIMK
HCCCCCCCCCCCCEECCCCCCEEEECCHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHH
RYKQQEPEAIHCYTQLIDHMARSFAGLVNVLDPDIIVLGGGLSNIDELYRDLPTATARHV
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCHHHHHHH
FSDSAQVHFAKAVFGDSSGIRGAAWL
HCCCCCEEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MQQDVSREHTINNGSSMADHDEHPIEQKEFYMLIGLDIGGTKIEICVLDKQGSMLYRQRI
CCCCCCHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEEEECCCCCHHHHHHC
ATPDNYSQFVDCVCSLIVDAEQATQPVDSIGIGLPGAVSPVTGLIKNANCTFLNGQDLSS
CCCCCHHHHHHHHHHHHHCCHHHHCCHHHHCCCCCCCHHHHHHHHHCCCEEEECCCCCCH
DLQYRLGREVKLANDANCFALSEAIDGAGKESMVVFGAILGTGCGGSIVVNRQVLVGPNA
HHHHHCCCEEEECCCCCCEEHHHHHCCCCCCCEEEEEHHHHCCCCCEEEEECEEEECCCH
ICGEWGHNPLPGYHLEQDGAARYCYCGRQNCIERFISGSGFQDSYQALTGECITASEIMK
HCCCCCCCCCCCCEECCCCCCEEEECCHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHHH
RYKQQEPEAIHCYTQLIDHMARSFAGLVNVLDPDIIVLGGGLSNIDELYRDLPTATARHV
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCHHHHHHH
FSDSAQVHFAKAVFGDSSGIRGAAWL
HCCCCCEEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]