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The map label for this gene is manA-1 [H]

Identifier: 227080484

GI number: 227080484

Start: 259236

End: 260435

Strand: Direct

Name: manA-1 [H]

Synonym: VCM66_0254

Alternate gene names: 227080484

Gene position: 259236-260435 (Clockwise)

Preceding gene: 227080483

Following gene: 227080485

Centisome position: 8.96

GC content: 41.25

Gene sequence:

>1200_bases
ATGAACACCGCAATTCCAGCTCCATTTTTTATTATGCATAACGTTATCCAATCATACGCATGGGGCAGTATTGACTCAAT
AAACCAGTTGTTTGACATTCCTAATCCAAAGGGAGAACCTCAAGCTGAAGTATGGATGGGTACTCACCCGAATGGTTGCT
CATACGTCGAATTTTCTGGAGAAAAAATAAAACTCAGTACTCTAATAGAACAAAAAAAAACTGACTTTCTCTCAATCCAA
ACAGTAGAACAGTTTGGTGAGCTACCTTATTTGTTTAAAATTCTTGCTGCAAATCAAGCGCTTTCGATTCAAGTTCACCC
AAGCAAAGCAGAAGCAGAGGAAGGATTTGCTCGCGAAAATGCCCAAGGAATTTTGATAAACGCTACAAATCGAAACTATA
AAGATCCTAACCATAAACCAGAACTGGTTTACGCGCTTACCAATTATCAAGCCATGAATGGTTTTCGTCCATTAAGTGAC
ATTATTTCACTATTTAGCGCCTTGGCTATCGATGAAATAGCTTCGCTATTGAGCCACCTTAAACGAAACCAAAACGAAAC
AGGGCTCGAACACTTTTTTACTAAATTACTCTCACTCTGTGGTGAAAAAAAAAGACGCGTTTTAGAACAATTGCTTAGCT
ATGCTCGATCGCATGCAAAACAACCTATATTTGCCTTAATCGAAGAACTAGCGACACAATACCCAAATGACATTGGCCTT
TTCGCGCCTTTGATGCTGCATGTTTTAACCCTTCAACCTGGCGAAGCAATGTTCTTAGATGCGCGCACACCTCATGCTTA
CTTGAAAGGTACAGCTTTGGAAATCATGGCTAATTCCGATAATGTGCTGCGTGCTGGGCTCACTCCAAAGCATATTGATG
TGGTAGAACTTGCTAAATGCACTCTATTTAAGGAAAAATCTGAAGCCACCTTATTGCTCACACCAATTCAACAAGGAGAT
ATGCTCAGTTTCCCAGTGCCTGTAGCGGATTTTAAGTTCGCGATTTTCCCACGACCGCAGCAAGCTAATATCACAGTTAG
TAGCGCCGAAATTTTACTGCCAATTGATTGCGATGCTACTTTAGTTAGTCCTTGCGGCACCGTTCTTACAGTACACAAAG
GGCAATCGGTCTTTATTCCCGCCTATACGGAAAAATACTGCTTAAGTGCAGCAGGACGTGTCGCGCGAGCATACAATTAA

Upstream 100 bases:

>100_bases
TTCTATATTTTAGGTAGGAATCTAAATGTTTTGATATTGCATGCTGTAATTGAATATGTGCAGTTTATAATGCAAGTATT
TTTTTGACTAGGGACCATAT

Downstream 100 bases:

>100_bases
TAATGGTTCGAGCATGGCGGACCATGATGAGCATCCCATAGAGCAAAAGGAATTCTACATGCTGATTGGATTGGATATTG
GTGGCACAAAAATAGAAATA

Product: mannose-6-phosphate isomerase

Products: NA

Alternate protein names: Phosphohexomutase; Phosphomannose isomerase; PMI [H]

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MNTAIPAPFFIMHNVIQSYAWGSIDSINQLFDIPNPKGEPQAEVWMGTHPNGCSYVEFSGEKIKLSTLIEQKKTDFLSIQ
TVEQFGELPYLFKILAANQALSIQVHPSKAEAEEGFARENAQGILINATNRNYKDPNHKPELVYALTNYQAMNGFRPLSD
IISLFSALAIDEIASLLSHLKRNQNETGLEHFFTKLLSLCGEKKRRVLEQLLSYARSHAKQPIFALIEELATQYPNDIGL
FAPLMLHVLTLQPGEAMFLDARTPHAYLKGTALEIMANSDNVLRAGLTPKHIDVVELAKCTLFKEKSEATLLLTPIQQGD
MLSFPVPVADFKFAIFPRPQQANITVSSAEILLPIDCDATLVSPCGTVLTVHKGQSVFIPAYTEKYCLSAAGRVARAYN

Sequences:

>Translated_399_residues
MNTAIPAPFFIMHNVIQSYAWGSIDSINQLFDIPNPKGEPQAEVWMGTHPNGCSYVEFSGEKIKLSTLIEQKKTDFLSIQ
TVEQFGELPYLFKILAANQALSIQVHPSKAEAEEGFARENAQGILINATNRNYKDPNHKPELVYALTNYQAMNGFRPLSD
IISLFSALAIDEIASLLSHLKRNQNETGLEHFFTKLLSLCGEKKRRVLEQLLSYARSHAKQPIFALIEELATQYPNDIGL
FAPLMLHVLTLQPGEAMFLDARTPHAYLKGTALEIMANSDNVLRAGLTPKHIDVVELAKCTLFKEKSEATLLLTPIQQGD
MLSFPVPVADFKFAIFPRPQQANITVSSAEILLPIDCDATLVSPCGTVLTVHKGQSVFIPAYTEKYCLSAAGRVARAYN
>Mature_399_residues
MNTAIPAPFFIMHNVIQSYAWGSIDSINQLFDIPNPKGEPQAEVWMGTHPNGCSYVEFSGEKIKLSTLIEQKKTDFLSIQ
TVEQFGELPYLFKILAANQALSIQVHPSKAEAEEGFARENAQGILINATNRNYKDPNHKPELVYALTNYQAMNGFRPLSD
IISLFSALAIDEIASLLSHLKRNQNETGLEHFFTKLLSLCGEKKRRVLEQLLSYARSHAKQPIFALIEELATQYPNDIGL
FAPLMLHVLTLQPGEAMFLDARTPHAYLKGTALEIMANSDNVLRAGLTPKHIDVVELAKCTLFKEKSEATLLLTPIQQGD
MLSFPVPVADFKFAIFPRPQQANITVSSAEILLPIDCDATLVSPCGTVLTVHKGQSVFIPAYTEKYCLSAAGRVARAYN

Specific function: Involved in the conversion of glucose to GDP-L-fucose, which can be converted to L-fucose, a capsular polysaccharide [H]

COG id: COG1482

COG function: function code G; Phosphomannose isomerase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 1 family [H]

Homologues:

Organism=Homo sapiens, GI4505235, Length=412, Percent_Identity=35.1941747572816, Blast_Score=188, Evalue=9e-48,
Organism=Escherichia coli, GI1787899, Length=388, Percent_Identity=46.6494845360825, Blast_Score=337, Evalue=6e-94,
Organism=Caenorhabditis elegans, GI71997620, Length=392, Percent_Identity=32.1428571428571, Blast_Score=175, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17557650, Length=387, Percent_Identity=24.8062015503876, Blast_Score=131, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6320839, Length=316, Percent_Identity=33.5443037974684, Blast_Score=154, Evalue=2e-38,
Organism=Drosophila melanogaster, GI21356061, Length=334, Percent_Identity=33.8323353293413, Blast_Score=157, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR001250
- InterPro:   IPR016305
- InterPro:   IPR018050
- InterPro:   IPR014710 [H]

Pfam domain/function: PF01238 PMI_typeI [H]

EC number: =5.3.1.8 [H]

Molecular weight: Translated: 44250; Mature: 44250

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00965 PMI_I_1 ; PS00966 PMI_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTAIPAPFFIMHNVIQSYAWGSIDSINQLFDIPNPKGEPQAEVWMGTHPNGCSYVEFSG
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCEEEECC
EKIKLSTLIEQKKTDFLSIQTVEQFGELPYLFKILAANQALSIQVHPSKAEAEEGFAREN
CEEEHHHHHHHHCCCCEEHHHHHHHCCCHHHHHHHHCCCEEEEEECCCHHHHHHCHHHCC
AQGILINATNRNYKDPNHKPELVYALTNYQAMNGFRPLSDIISLFSALAIDEIASLLSHL
CCEEEEECCCCCCCCCCCCCCEEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KRNQNETGLEHFFTKLLSLCGEKKRRVLEQLLSYARSHAKQPIFALIEELATQYPNDIGL
HCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHH
FAPLMLHVLTLQPGEAMFLDARTPHAYLKGTALEIMANSDNVLRAGLTPKHIDVVELAKC
HHHHHHHHHHCCCCCEEEEECCCCCHHHCCCEEEEEECCCCEEEECCCCCCCHHHHHHHH
TLFKEKSEATLLLTPIQQGDMLSFPVPVADFKFAIFPRPQQANITVSSAEILLPIDCDAT
HHEECCCCCEEEEEECCCCCEEECCCCCCCCEEEEECCCCCCEEEEECCEEEEEECCCCH
LVSPCGTVLTVHKGQSVFIPAYTEKYCLSAAGRVARAYN
HHCCCCCEEEEECCCEEEEECHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNTAIPAPFFIMHNVIQSYAWGSIDSINQLFDIPNPKGEPQAEVWMGTHPNGCSYVEFSG
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCEEEECC
EKIKLSTLIEQKKTDFLSIQTVEQFGELPYLFKILAANQALSIQVHPSKAEAEEGFAREN
CEEEHHHHHHHHCCCCEEHHHHHHHCCCHHHHHHHHCCCEEEEEECCCHHHHHHCHHHCC
AQGILINATNRNYKDPNHKPELVYALTNYQAMNGFRPLSDIISLFSALAIDEIASLLSHL
CCEEEEECCCCCCCCCCCCCCEEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KRNQNETGLEHFFTKLLSLCGEKKRRVLEQLLSYARSHAKQPIFALIEELATQYPNDIGL
HCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHH
FAPLMLHVLTLQPGEAMFLDARTPHAYLKGTALEIMANSDNVLRAGLTPKHIDVVELAKC
HHHHHHHHHHCCCCCEEEEECCCCCHHHCCCEEEEEECCCCEEEECCCCCCCHHHHHHHH
TLFKEKSEATLLLTPIQQGDMLSFPVPVADFKFAIFPRPQQANITVSSAEILLPIDCDAT
HHEECCCCCEEEEEECCCCCEEECCCCCCCCEEEEECCCCCCEEEEECCEEEEEECCCCH
LVSPCGTVLTVHKGQSVFIPAYTEKYCLSAAGRVARAYN
HHCCCCCEEEEECCCEEEEECHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1879695; 11677609 [H]