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The map label for this gene is 227080479

Identifier: 227080479

GI number: 227080479

Start: 253795

End: 255162

Strand: Direct

Name: 227080479

Synonym: VCM66_0249

Alternate gene names: NA

Gene position: 253795-255162 (Clockwise)

Preceding gene: 227080478

Following gene: 227080483

Centisome position: 8.77

GC content: 55.19

Gene sequence:

>1368_bases
TTGCTGAGGCGAGCTAACCACGGCAAGGTGGCGCAAAACAGGAGAAACAACATGGAAGTGGTTCATCACGGAGGGAAGGC
GAGTGTCACGGGTTCATGCCATGAATTGCGAGCCGATGGGCAAGCGCTGCTGATTGATTGCGGCTTGTTTCAAGGGGCGG
ATGAACGCCCGTTGGCTGTGGAGTTTGCGCTGGGGCATGTGGATGCTCTGATCTTAACTCATGCGCACATCGACCATATT
GGCCGTTTGCCTTGGTTATTGGCGGCGGGCCTTAAGCAGCCGATTTACTCTACGGCGGCAACGGCGGAGCTGGTGCCATT
GATGCTGGAAGATGGTTTAAAGCTGCAGTTAGGGATGTCTCCCAAGCAGAGTGAGCGCGTACTGACTGAGGTGCGCCGTT
TGCTGCGGGTGCAAGATTACCAAAAGTGGTTTGCAGTGCAGCCTAAGCGTGCTGATTCGCTCTGGGTGCGCTTTCAGCCT
GCGGGGCATATTTTGGGCTCGGCGTATGTGGAAATCCGGCGTCCGAATGGTGAGGTGGTGGTGTTTTCAGGGGATTTGGG
GCCAAGCCATACGCCTTTGTTGCCTGATCCGCAGTCGCCAGAGCGGGCAGATTATCTGTTTATTGAAACCACTTATGGCG
ATAAGCAGCACGAGGATGTGCAGAGCCGAGGGCAGCGGCTGCGCGCGATGATTGAGCGCTCACTGACTGACGGCGGTGCG
ATTTTGATCCCGGCGTTCAGTGTCGGTCGCACGCAAGAGCTGCTCTTTGATATTGAGCAGCTCATCTTTTCTCAGCAAAT
CGATGCTAACTTGCCGATTATTCTCGATTCGCCGATGGCGCAGCGAGTGACGCGTTCTTATCGTCGCTTTAAGCAGTTAT
GGGGACGAGAAGCCAAAGCGCGGCTGCAAATGCATCGGCATCCGTTGGCGTTTGAGCAGTGTATTACGGTGGAAGATCAC
CGAACCCATGAGCGCTTGGTCAACCGTTTAGCCTCAACCGGAGAGGCGGCGATTGTGGTGGCGGCGTCGGGGATGTGTCA
GGGCGGCCGCATTATGGATTATTTAAAAGCGCTGCTGCCGGATAAGCGCACTGACCTGATTTTGGCCGGCTTTCAAGCAG
AAGGCACGCTTGGGCGCAGTATTCAAAGTGGTCAACCTTCGGTCTGGATTGAGGGGACTGAAGTGGAAGTCAACGCGCAT
ATTCATACGATGTCCGGTTATTCCGCTCACGCCGATAAGGCCGATTTGCTGCGCTTTATTACCGGCATTCCTGAAAAGCC
CAAGCAAGTGCATCTGATCCACGGTGAAGCCCCAGCCAAGCAAGCGTTCGCGGCTGAGCTTACTCAACTCGGATATTCGG
TGCTTTGA

Upstream 100 bases:

>100_bases
TACTCTTGTCGATGTAGTTATTTACGCACTGATTTGTCGATGGGCTGTTGCTTATCGCTGAGTTTGTGGTGATTGGCTCG
CAGTTTGGTGGCTCGGTGCG

Downstream 100 bases:

>100_bases
CTGGGAAGCTTGGTTTGATGTGAAATACCCCCTCCTGTCCTCCCCCTAGAAGGGGGAGGTGCAAGAGCCATCTCTTTATC
AATCAGCAAGATTAAGCTTG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MLRRANHGKVAQNRRNNMEVVHHGGKASVTGSCHELRADGQALLIDCGLFQGADERPLAVEFALGHVDALILTHAHIDHI
GRLPWLLAAGLKQPIYSTAATAELVPLMLEDGLKLQLGMSPKQSERVLTEVRRLLRVQDYQKWFAVQPKRADSLWVRFQP
AGHILGSAYVEIRRPNGEVVVFSGDLGPSHTPLLPDPQSPERADYLFIETTYGDKQHEDVQSRGQRLRAMIERSLTDGGA
ILIPAFSVGRTQELLFDIEQLIFSQQIDANLPIILDSPMAQRVTRSYRRFKQLWGREAKARLQMHRHPLAFEQCITVEDH
RTHERLVNRLASTGEAAIVVAASGMCQGGRIMDYLKALLPDKRTDLILAGFQAEGTLGRSIQSGQPSVWIEGTEVEVNAH
IHTMSGYSAHADKADLLRFITGIPEKPKQVHLIHGEAPAKQAFAAELTQLGYSVL

Sequences:

>Translated_455_residues
MLRRANHGKVAQNRRNNMEVVHHGGKASVTGSCHELRADGQALLIDCGLFQGADERPLAVEFALGHVDALILTHAHIDHI
GRLPWLLAAGLKQPIYSTAATAELVPLMLEDGLKLQLGMSPKQSERVLTEVRRLLRVQDYQKWFAVQPKRADSLWVRFQP
AGHILGSAYVEIRRPNGEVVVFSGDLGPSHTPLLPDPQSPERADYLFIETTYGDKQHEDVQSRGQRLRAMIERSLTDGGA
ILIPAFSVGRTQELLFDIEQLIFSQQIDANLPIILDSPMAQRVTRSYRRFKQLWGREAKARLQMHRHPLAFEQCITVEDH
RTHERLVNRLASTGEAAIVVAASGMCQGGRIMDYLKALLPDKRTDLILAGFQAEGTLGRSIQSGQPSVWIEGTEVEVNAH
IHTMSGYSAHADKADLLRFITGIPEKPKQVHLIHGEAPAKQAFAAELTQLGYSVL
>Mature_455_residues
MLRRANHGKVAQNRRNNMEVVHHGGKASVTGSCHELRADGQALLIDCGLFQGADERPLAVEFALGHVDALILTHAHIDHI
GRLPWLLAAGLKQPIYSTAATAELVPLMLEDGLKLQLGMSPKQSERVLTEVRRLLRVQDYQKWFAVQPKRADSLWVRFQP
AGHILGSAYVEIRRPNGEVVVFSGDLGPSHTPLLPDPQSPERADYLFIETTYGDKQHEDVQSRGQRLRAMIERSLTDGGA
ILIPAFSVGRTQELLFDIEQLIFSQQIDANLPIILDSPMAQRVTRSYRRFKQLWGREAKARLQMHRHPLAFEQCITVEDH
RTHERLVNRLASTGEAAIVVAASGMCQGGRIMDYLKALLPDKRTDLILAGFQAEGTLGRSIQSGQPSVWIEGTEVEVNAH
IHTMSGYSAHADKADLLRFITGIPEKPKQVHLIHGEAPAKQAFAAELTQLGYSVL

Specific function: Has endoribonuclease activity towards 23S and 16S rRNA (in vitro) [H]

COG id: COG1236

COG function: function code J; Predicted exonuclease of the beta-lactamase fold involved in RNA processing

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family [H]

Homologues:

Organism=Homo sapiens, GI33300633, Length=447, Percent_Identity=25.503355704698, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI7706427, Length=419, Percent_Identity=26.491646778043, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI32566029, Length=427, Percent_Identity=28.1030444964871, Blast_Score=154, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI32564696, Length=454, Percent_Identity=25.3303964757709, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323307, Length=437, Percent_Identity=24.9427917620137, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24648013, Length=422, Percent_Identity=27.4881516587678, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI21358523, Length=442, Percent_Identity=26.0180995475113, Blast_Score=149, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022712
- InterPro:   IPR001279
- InterPro:   IPR011108 [H]

Pfam domain/function: PF10996 Beta-Casp; PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 50571; Mature: 50571

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRRANHGKVAQNRRNNMEVVHHGGKASVTGSCHELRADGQALLIDCGLFQGADERPLAV
CCCCCCCCCHHHCCCCCCEEEEECCCCCCCCCHHHHCCCCCEEEEEECCCCCCCCCCEEE
EFALGHVDALILTHAHIDHIGRLPWLLAAGLKQPIYSTAATAELVPLMLEDGLKLQLGMS
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEECCC
PKQSERVLTEVRRLLRVQDYQKWFAVQPKRADSLWVRFQPAGHILGSAYVEIRRPNGEVV
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCEE
VFSGDLGPSHTPLLPDPQSPERADYLFIETTYGDKQHEDVQSRGQRLRAMIERSLTDGGA
EEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCE
ILIPAFSVGRTQELLFDIEQLIFSQQIDANLPIILDSPMAQRVTRSYRRFKQLWGREAKA
EEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHCHHHHH
RLQMHRHPLAFEQCITVEDHRTHERLVNRLASTGEAAIVVAASGMCQGGRIMDYLKALLP
HHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHCC
DKRTDLILAGFQAEGTLGRSIQSGQPSVWIEGTEVEVNAHIHTMSGYSAHADKADLLRFI
CCCCCEEEEECCCCCCCCCCCCCCCCCEEEECCEEEEEEEEEEECCCCCCCCHHHHHHHH
TGIPEKPKQVHLIHGEAPAKQAFAAELTQLGYSVL
HCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLRRANHGKVAQNRRNNMEVVHHGGKASVTGSCHELRADGQALLIDCGLFQGADERPLAV
CCCCCCCCCHHHCCCCCCEEEEECCCCCCCCCHHHHCCCCCEEEEEECCCCCCCCCCEEE
EFALGHVDALILTHAHIDHIGRLPWLLAAGLKQPIYSTAATAELVPLMLEDGLKLQLGMS
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEECCC
PKQSERVLTEVRRLLRVQDYQKWFAVQPKRADSLWVRFQPAGHILGSAYVEIRRPNGEVV
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCEE
VFSGDLGPSHTPLLPDPQSPERADYLFIETTYGDKQHEDVQSRGQRLRAMIERSLTDGGA
EEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCE
ILIPAFSVGRTQELLFDIEQLIFSQQIDANLPIILDSPMAQRVTRSYRRFKQLWGREAKA
EEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHHHHCHHHHH
RLQMHRHPLAFEQCITVEDHRTHERLVNRLASTGEAAIVVAASGMCQGGRIMDYLKALLP
HHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHCC
DKRTDLILAGFQAEGTLGRSIQSGQPSVWIEGTEVEVNAHIHTMSGYSAHADKADLLRFI
CCCCCEEEEECCCCCCCCCCCCCCCCCEEEECCEEEEEEEEEEECCCCCCCCHHHHHHHH
TGIPEKPKQVHLIHGEAPAKQAFAAELTQLGYSVL
HCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA