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The map label for this gene is rfbL [H]

Identifier: 227080468

GI number: 227080468

Start: 240741

End: 242156

Strand: Direct

Name: rfbL [H]

Synonym: VCM66_0237

Alternate gene names: 227080468

Gene position: 240741-242156 (Clockwise)

Preceding gene: 227080467

Following gene: 227080469

Centisome position: 8.32

GC content: 37.71

Gene sequence:

>1416_bases
ATGTCTGTGGTTGAGAGAATATTTAACCATTCCGAGTCAAGACCGGATTGTATAGCGGTCTATGAAAATGATCGAACAAT
TAGTTATGAAAATCTTGCTTCGGATATAAAAAAAACCGCTGCCAAGTTAGTATCCTTAGGTGTAACATCTGAGGATGCTG
TACTTGTAAGATCTAATAATAGCTATAGTTTCATACTGCTTTATTTTTCTATTCACTATGTCGGTGCTAAATTTGTTAAT
GTTGCTCCTGACTCAGACGTAAGCTACGTGTCTTTTATTGAAGATAAAGTAAATCCAAAACTATTTATTGAAAAAAGCCA
GGATTTTATTAGAAACATAGATAGTTATACATTTGATAATATATTTCCCCCTCAGGCGAGGGGGATCGCAGATATTATGT
TCACGAGTGGTACTACTGGTGAGCCGAAGGGAGTTTTGTTAAGCCATAAAAGTTTAGTGTTAGCAACTGAACACATTATA
TCTCATGTAAAAAATACCAATGAAGATGTTGAGCTTTTGTTAATGCCACTAAGCCACTCATTTGCTATGGCTAGAATGAG
GACTAGTTTATTTGCAGGTGGTGCTATCGTAGTTGGCTGCTCTTTTAAGCAATTAAAGTCGGTATTCAAAGCTATAGAGT
TTTACAAAGTTACAGGGCTAGGTCTCGTTCCTTCGGCTTGGTCATATATTACTTTAATGACCAAAGACCTCATACGTAAG
TATTCAGAACAGCTAAATTTTATAGAGTTCGGAAGTGCACACTTCCCTTTCGAGGATAAAAAGCAGGTCGCAGAGTGGTT
TCCAAACACTAATGTAGTTATGCACTATGGCTTAACGGAAGTGTCTCGAGCAACTTTCATTGATTTTCATAATGATGATA
TCAACGCGGTTGGTCATAGATATCGCGGAGCTGATTTTAAGATCATCGATAATAAAGGAGCGGAAGTAATTGAAGGAGAA
GAGGGTGAAATCGTTTTTAAAGCACCATGGATGTTGGATGGCTATTTTGAGAACAGCCAATTAACGTCAGATTGTTTTGT
AGAAGGTTACTTTAAAACAGGTGATTTAGGCCGTGTTGTTGGGGACTACCTATTTTTAACAGGCCGCTTAAAAGAAATAA
TTAACGTTGGCGGCAAAAAAGTAAGCCCTGATCAGGTGGAGAAAGTATTATCCGAAGCTCTAGGTGTTCAAGAATGTGCA
TGCGCTGCTTTATCAGATGCCAATATGGGTGAAGTGGTTCAAGCCTATATTGTTGTAAAATCTGGTTGGGATTGCGAAAA
TGTAATTTCAAATATAAAAGAGACCATAAATGGCCAGTTGCCGATGCATATGAGGCCTAAAAAATATAGTATAGTCAGTG
CTTTGCCAAAGACCGTTTCAGGGAAAGTGCAAAGATACAAACTGTCTTCTGAATAG

Upstream 100 bases:

>100_bases
TTGAATCGGATTTGGGAGTAGTTTTGGATATCGAACAAGTTCTTGAAATAGAAGATGTTGAAGATATTATCGATGCTGTA
ATTAATAACGAGTAAATATT

Downstream 100 bases:

>100_bases
GAACATTCATGCTAAAACTACGTTTCCCACTACCTGTATTCCGTAACGCCCGAGAAATCGCTAATGGCAGGAATGCCCGA
ATGGCGTTAAAAGGTTTAGT

Product: rfbL protein

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MSVVERIFNHSESRPDCIAVYENDRTISYENLASDIKKTAAKLVSLGVTSEDAVLVRSNNSYSFILLYFSIHYVGAKFVN
VAPDSDVSYVSFIEDKVNPKLFIEKSQDFIRNIDSYTFDNIFPPQARGIADIMFTSGTTGEPKGVLLSHKSLVLATEHII
SHVKNTNEDVELLLMPLSHSFAMARMRTSLFAGGAIVVGCSFKQLKSVFKAIEFYKVTGLGLVPSAWSYITLMTKDLIRK
YSEQLNFIEFGSAHFPFEDKKQVAEWFPNTNVVMHYGLTEVSRATFIDFHNDDINAVGHRYRGADFKIIDNKGAEVIEGE
EGEIVFKAPWMLDGYFENSQLTSDCFVEGYFKTGDLGRVVGDYLFLTGRLKEIINVGGKKVSPDQVEKVLSEALGVQECA
CAALSDANMGEVVQAYIVVKSGWDCENVISNIKETINGQLPMHMRPKKYSIVSALPKTVSGKVQRYKLSSE

Sequences:

>Translated_471_residues
MSVVERIFNHSESRPDCIAVYENDRTISYENLASDIKKTAAKLVSLGVTSEDAVLVRSNNSYSFILLYFSIHYVGAKFVN
VAPDSDVSYVSFIEDKVNPKLFIEKSQDFIRNIDSYTFDNIFPPQARGIADIMFTSGTTGEPKGVLLSHKSLVLATEHII
SHVKNTNEDVELLLMPLSHSFAMARMRTSLFAGGAIVVGCSFKQLKSVFKAIEFYKVTGLGLVPSAWSYITLMTKDLIRK
YSEQLNFIEFGSAHFPFEDKKQVAEWFPNTNVVMHYGLTEVSRATFIDFHNDDINAVGHRYRGADFKIIDNKGAEVIEGE
EGEIVFKAPWMLDGYFENSQLTSDCFVEGYFKTGDLGRVVGDYLFLTGRLKEIINVGGKKVSPDQVEKVLSEALGVQECA
CAALSDANMGEVVQAYIVVKSGWDCENVISNIKETINGQLPMHMRPKKYSIVSALPKTVSGKVQRYKLSSE
>Mature_470_residues
SVVERIFNHSESRPDCIAVYENDRTISYENLASDIKKTAAKLVSLGVTSEDAVLVRSNNSYSFILLYFSIHYVGAKFVNV
APDSDVSYVSFIEDKVNPKLFIEKSQDFIRNIDSYTFDNIFPPQARGIADIMFTSGTTGEPKGVLLSHKSLVLATEHIIS
HVKNTNEDVELLLMPLSHSFAMARMRTSLFAGGAIVVGCSFKQLKSVFKAIEFYKVTGLGLVPSAWSYITLMTKDLIRKY
SEQLNFIEFGSAHFPFEDKKQVAEWFPNTNVVMHYGLTEVSRATFIDFHNDDINAVGHRYRGADFKIIDNKGAEVIEGEE
GEIVFKAPWMLDGYFENSQLTSDCFVEGYFKTGDLGRVVGDYLFLTGRLKEIINVGGKKVSPDQVEKVLSEALGVQECAC
AALSDANMGEVVQAYIVVKSGWDCENVISNIKETINGQLPMHMRPKKYSIVSALPKTVSGKVQRYKLSSE

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=526, Percent_Identity=22.4334600760456, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI38505220, Length=376, Percent_Identity=27.1276595744681, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI58082049, Length=360, Percent_Identity=27.2222222222222, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI157311624, Length=360, Percent_Identity=26.3888888888889, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI157311622, Length=360, Percent_Identity=26.3888888888889, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI42544132, Length=364, Percent_Identity=25.5494505494505, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI122937307, Length=366, Percent_Identity=25.6830601092896, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI115511026, Length=359, Percent_Identity=26.4623955431755, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI28416953, Length=510, Percent_Identity=21.9607843137255, Blast_Score=73, Evalue=6e-13,
Organism=Escherichia coli, GI145693145, Length=510, Percent_Identity=23.1372549019608, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1786810, Length=516, Percent_Identity=23.2558139534884, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1788595, Length=470, Percent_Identity=22.9787234042553, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1788107, Length=356, Percent_Identity=26.123595505618, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI221142682, Length=500, Percent_Identity=22.8, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1786801, Length=509, Percent_Identity=22.2003929273084, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786529, Length=552, Percent_Identity=23.0072463768116, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17557194, Length=531, Percent_Identity=27.4952919020716, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17531443, Length=517, Percent_Identity=26.4990328820116, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17538037, Length=529, Percent_Identity=25.3308128544423, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71996755, Length=481, Percent_Identity=23.9085239085239, Blast_Score=128, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17559526, Length=351, Percent_Identity=27.3504273504274, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17558820, Length=486, Percent_Identity=25.3086419753086, Blast_Score=112, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI71994703, Length=523, Percent_Identity=22.3709369024857, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71994694, Length=523, Percent_Identity=22.3709369024857, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71994690, Length=523, Percent_Identity=22.3709369024857, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71985884, Length=350, Percent_Identity=26.2857142857143, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI32563687, Length=478, Percent_Identity=24.0585774058577, Blast_Score=98, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17560140, Length=360, Percent_Identity=24.7222222222222, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17560308, Length=351, Percent_Identity=24.5014245014245, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6319699, Length=370, Percent_Identity=29.4594594594595, Blast_Score=112, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6323182, Length=372, Percent_Identity=23.9247311827957, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6319264, Length=411, Percent_Identity=23.3576642335766, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21355181, Length=480, Percent_Identity=26.6666666666667, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24581924, Length=361, Percent_Identity=27.4238227146814, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI18859661, Length=354, Percent_Identity=25.1412429378531, Blast_Score=99, Evalue=7e-21,
Organism=Drosophila melanogaster, GI281365686, Length=316, Percent_Identity=26.2658227848101, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI20130357, Length=515, Percent_Identity=22.7184466019417, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24648260, Length=468, Percent_Identity=22.8632478632479, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21358303, Length=325, Percent_Identity=24.6153846153846, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21356947, Length=297, Percent_Identity=26.5993265993266, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24653035, Length=506, Percent_Identity=22.5296442687747, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24648253, Length=510, Percent_Identity=22.5490196078431, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24667955, Length=367, Percent_Identity=25.6130790190736, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI62472339, Length=367, Percent_Identity=25.6130790190736, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24667959, Length=370, Percent_Identity=25.6756756756757, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24648255, Length=510, Percent_Identity=22.3529411764706, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI19922652, Length=482, Percent_Identity=21.1618257261411, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI21356441, Length=463, Percent_Identity=21.8142548596112, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI21355225, Length=374, Percent_Identity=24.0641711229947, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24648257, Length=512, Percent_Identity=22.0703125, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 52573; Mature: 52441

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVERIFNHSESRPDCIAVYENDRTISYENLASDIKKTAAKLVSLGVTSEDAVLVRSNN
CCHHHHHHCCCCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
SYSFILLYFSIHYVGAKFVNVAPDSDVSYVSFIEDKVNPKLFIEKSQDFIRNIDSYTFDN
CEEEEEEEEEHHHHCCEEEEECCCCCCHHHHHHHHCCCCEEEEECCHHHHHHCCCCCCCC
IFPPQARGIADIMFTSGTTGEPKGVLLSHKSLVLATEHIISHVKNTNEDVELLLMPLSHS
CCCCCCCCCEEEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCEEEEEEECCHH
FAMARMRTSLFAGGAIVVGCSFKQLKSVFKAIEFYKVTGLGLVPSAWSYITLMTKDLIRK
HHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHH
YSEQLNFIEFGSAHFPFEDKKQVAEWFPNTNVVMHYGLTEVSRATFIDFHNDDINAVGHR
HHHHCCEEEECCCCCCCCHHHHHHHHCCCCCEEEEECCHHHCCEEEEEECCCCHHHHCCC
YRGADFKIIDNKGAEVIEGEEGEIVFKAPWMLDGYFENSQLTSDCFVEGYFKTGDLGRVV
CCCCCEEEECCCCCCEEECCCCCEEEECCCEEECEECCCCCCCCCEEEEEECCCCHHHHH
GDYLFLTGRLKEIINVGGKKVSPDQVEKVLSEALGVQECACAALSDANMGEVVQAYIVVK
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHEEEEEEEE
SGWDCENVISNIKETINGQLPMHMRPKKYSIVSALPKTVSGKVQRYKLSSE
CCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHHCCCEEEEEECCC
>Mature Secondary Structure 
SVVERIFNHSESRPDCIAVYENDRTISYENLASDIKKTAAKLVSLGVTSEDAVLVRSNN
CHHHHHHCCCCCCCCEEEEEECCCEEEHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
SYSFILLYFSIHYVGAKFVNVAPDSDVSYVSFIEDKVNPKLFIEKSQDFIRNIDSYTFDN
CEEEEEEEEEHHHHCCEEEEECCCCCCHHHHHHHHCCCCEEEEECCHHHHHHCCCCCCCC
IFPPQARGIADIMFTSGTTGEPKGVLLSHKSLVLATEHIISHVKNTNEDVELLLMPLSHS
CCCCCCCCCEEEEEECCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCEEEEEEECCHH
FAMARMRTSLFAGGAIVVGCSFKQLKSVFKAIEFYKVTGLGLVPSAWSYITLMTKDLIRK
HHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHH
YSEQLNFIEFGSAHFPFEDKKQVAEWFPNTNVVMHYGLTEVSRATFIDFHNDDINAVGHR
HHHHCCEEEECCCCCCCCHHHHHHHHCCCCCEEEEECCHHHCCEEEEEECCCCHHHHCCC
YRGADFKIIDNKGAEVIEGEEGEIVFKAPWMLDGYFENSQLTSDCFVEGYFKTGDLGRVV
CCCCCEEEECCCCCCEEECCCCCEEEECCCEEECEECCCCCCCCCEEEEEECCCCHHHHH
GDYLFLTGRLKEIINVGGKKVSPDQVEKVLSEALGVQECACAALSDANMGEVVQAYIVVK
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHEEEEEEEE
SGWDCENVISNIKETINGQLPMHMRPKKYSIVSALPKTVSGKVQRYKLSSE
CCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCHHHCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663927 [H]