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The map label for this gene is adh1 [H]

Identifier: 227080469

GI number: 227080469

Start: 242165

End: 243289

Strand: Direct

Name: adh1 [H]

Synonym: VCM66_0238

Alternate gene names: 227080469

Gene position: 242165-243289 (Clockwise)

Preceding gene: 227080468

Following gene: 227080470

Centisome position: 8.37

GC content: 42.49

Gene sequence:

>1125_bases
ATGCTAAAACTACGTTTCCCACTACCTGTATTCCGTAACGCCCGAGAAATCGCTAATGGCAGGAATGCCCGAATGGCGTT
AAAAGGTTTAGTAGCTAACCGAGCTGCACTTATTATTAGCCCTTCCTTTGGGAGTTCTCAGTACTTTGAACAAGTCGTTC
GCCTTATTAACGCACAAAGTGTCCGGGTGATAGAGCGCTCTTGGCAAGGAGAACCAAGTGTAGAGGAACTTTCTGGAGTT
ATTGGAGAGCTTGAAGATTTTAAGCCAGACTATATTCTTGCACTTGGCGGCGGTTCTATCATTGATGGAGCTAAGCTCGC
TTGGTTATTCTACGAATGCCCAACTCTTGAATCAGAACTTCTATATCGACCATTTGCATTGCCATCCCTTCGTGGTCGCG
CAAAATTTGCTGCAATACCAACAACGGTAGGGGCTGGCTCTGAAGTTTCATCCGCTGCTGTCATGCTTGATAGTGTTACA
AACAGCAAAAAGGCAGTTGTCACTCATGATTTTTTACCAGACTTAGTGATCCTTGATCCTGACTTGGTTTCAGAAGTTCC
ACCTAAGGTATTGAAAACAACTGTGGCCGATGCTCTCTCTCACGCCATTGAAGGTTATGTCTCTTTGATTGATAACCCTT
TGATGAATACTTTTGCTGAGCAGGCGGTATCAATAATCTACCGCTATCGACATCAATTTTCCAATGATAATTGGAGCAGC
GAAATGCTTTCTGAACTGCAACAAGCTGCTATGTTTGCAGGGTGGGTTCAAAATCATTGCATAGTTGGTCTTTCACATGC
CATTGCTCACCAGCTAGGTACGTTTAATATAGGCCACGGATTGGCGAACGGTTTATTAATGCCTGCGGTGATCAACTTTA
ATTACCGCGAACATACAGCTGCAACTAAGTATGAACAATTGATCTATAAATCCGGGTTACCTAATAAAGAAGCATTATTA
GAACTTTTTGAAGTATTGGTAGGAGAACAGAAAACTGATATGCATTTTTCAGAACAACAGCTGACAACGATCGCTGAAAA
TGCTTTACTTGACCCTGCTGCTAAGTCAAATCCTGTTCGATTTACAGAAAACGATGTGAAAGAGATTGTGAAGCAATGCC
TGTAA

Upstream 100 bases:

>100_bases
TGCATATGAGGCCTAAAAAATATAGTATAGTCAGTGCTTTGCCAAAGACCGTTTCAGGGAAAGTGCAAAGATACAAACTG
TCTTCTGAATAGGAACATTC

Downstream 100 bases:

>100_bases
ATAACGAAAATCTGACCAGTGTACTTGATGCTCGCCCTTTTGAATTATCAGAAGAGCAAAAATCTCCACTATTTAAAGCG
AACTTACTTGCAGAGTTAGT

Product: putative iron-containing alcohol dehydrogenase

Products: Acetyl-CoA; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: BDH [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MLKLRFPLPVFRNAREIANGRNARMALKGLVANRAALIISPSFGSSQYFEQVVRLINAQSVRVIERSWQGEPSVEELSGV
IGELEDFKPDYILALGGGSIIDGAKLAWLFYECPTLESELLYRPFALPSLRGRAKFAAIPTTVGAGSEVSSAAVMLDSVT
NSKKAVVTHDFLPDLVILDPDLVSEVPPKVLKTTVADALSHAIEGYVSLIDNPLMNTFAEQAVSIIYRYRHQFSNDNWSS
EMLSELQQAAMFAGWVQNHCIVGLSHAIAHQLGTFNIGHGLANGLLMPAVINFNYREHTAATKYEQLIYKSGLPNKEALL
ELFEVLVGEQKTDMHFSEQQLTTIAENALLDPAAKSNPVRFTENDVKEIVKQCL

Sequences:

>Translated_374_residues
MLKLRFPLPVFRNAREIANGRNARMALKGLVANRAALIISPSFGSSQYFEQVVRLINAQSVRVIERSWQGEPSVEELSGV
IGELEDFKPDYILALGGGSIIDGAKLAWLFYECPTLESELLYRPFALPSLRGRAKFAAIPTTVGAGSEVSSAAVMLDSVT
NSKKAVVTHDFLPDLVILDPDLVSEVPPKVLKTTVADALSHAIEGYVSLIDNPLMNTFAEQAVSIIYRYRHQFSNDNWSS
EMLSELQQAAMFAGWVQNHCIVGLSHAIAHQLGTFNIGHGLANGLLMPAVINFNYREHTAATKYEQLIYKSGLPNKEALL
ELFEVLVGEQKTDMHFSEQQLTTIAENALLDPAAKSNPVRFTENDVKEIVKQCL
>Mature_374_residues
MLKLRFPLPVFRNAREIANGRNARMALKGLVANRAALIISPSFGSSQYFEQVVRLINAQSVRVIERSWQGEPSVEELSGV
IGELEDFKPDYILALGGGSIIDGAKLAWLFYECPTLESELLYRPFALPSLRGRAKFAAIPTTVGAGSEVSSAAVMLDSVT
NSKKAVVTHDFLPDLVILDPDLVSEVPPKVLKTTVADALSHAIEGYVSLIDNPLMNTFAEQAVSIIYRYRHQFSNDNWSS
EMLSELQQAAMFAGWVQNHCIVGLSHAIAHQLGTFNIGHGLANGLLMPAVINFNYREHTAATKYEQLIYKSGLPNKEALL
ELFEVLVGEQKTDMHFSEQQLTTIAENALLDPAAKSNPVRFTENDVKEIVKQCL

Specific function: This enzyme has activity using butanol and ethanol as substrates [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1787493, Length=294, Percent_Identity=31.6326530612245, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI87082107, Length=298, Percent_Identity=32.2147651006711, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI48994951, Length=325, Percent_Identity=28, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1789163, Length=311, Percent_Identity=27.3311897106109, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789386, Length=209, Percent_Identity=26.3157894736842, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17537053, Length=321, Percent_Identity=25.8566978193146, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321181, Length=352, Percent_Identity=24.7159090909091, Blast_Score=111, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.1; 1.2.1.10 [C]

Molecular weight: Translated: 41230; Mature: 41230

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLRFPLPVFRNAREIANGRNARMALKGLVANRAALIISPSFGSSQYFEQVVRLINAQS
CEEECCCCHHHHCHHHHHCCCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHCCHH
VRVIERSWQGEPSVEELSGVIGELEDFKPDYILALGGGSIIDGAKLAWLFYECPTLESEL
HHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCEECCCCEEEEEEECCCCCHHH
LYRPFALPSLRGRAKFAAIPTTVGAGSEVSSAAVMLDSVTNSKKAVVTHDFLPDLVILDP
HHCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCEEEECH
DLVSEVPPKVLKTTVADALSHAIEGYVSLIDNPLMNTFAEQAVSIIYRYRHQFSNDNWSS
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHH
EMLSELQQAAMFAGWVQNHCIVGLSHAIAHQLGTFNIGHGLANGLLMPAVINFNYREHTA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHEECCCHHHHH
ATKYEQLIYKSGLPNKEALLELFEVLVGEQKTDMHFSEQQLTTIAENALLDPAAKSNPVR
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCE
FTENDVKEIVKQCL
ECHHHHHHHHHHCC
>Mature Secondary Structure
MLKLRFPLPVFRNAREIANGRNARMALKGLVANRAALIISPSFGSSQYFEQVVRLINAQS
CEEECCCCHHHHCHHHHHCCCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHCCHH
VRVIERSWQGEPSVEELSGVIGELEDFKPDYILALGGGSIIDGAKLAWLFYECPTLESEL
HHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCEECCCCEEEEEEECCCCCHHH
LYRPFALPSLRGRAKFAAIPTTVGAGSEVSSAAVMLDSVTNSKKAVVTHDFLPDLVILDP
HHCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCEEEECH
DLVSEVPPKVLKTTVADALSHAIEGYVSLIDNPLMNTFAEQAVSIIYRYRHQFSNDNWSS
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHH
EMLSELQQAAMFAGWVQNHCIVGLSHAIAHQLGTFNIGHGLANGLLMPAVINFNYREHTA
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHEECCCHHHHH
ATKYEQLIYKSGLPNKEALLELFEVLVGEQKTDMHFSEQQLTTIAENALLDPAAKSNPVR
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCE
FTENDVKEIVKQCL
ECHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 1000 [C]

Specific activity: NA

Km value (mM): 0.15 {ethanol}} 7.05 {acetaldehyde}} 0.1 {acetaldehyde}} [C]

Substrates: Acetaldehyde; Coenzyme A; Nicotinamide adenine dinucleotide [C]

Specific reaction: Acetaldehyde + Coenzyme A + Nicotinamide adenine dinucleotide --> Acetyl-CoA + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: 8-Hydroxy quinoline-5-sulfonic acid; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH; NEM; o-Phenanthroline of Zn2+; PCMB; Pyrazole; Substituted pyrazoles 4-iodo-; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2673928 [H]