Click here to switch to the map view.

The map label for this gene is rfbB

Identifier: 227080461

GI number: 227080461

Start: 233917

End: 235308

Strand: Direct

Name: rfbB

Synonym: VCM66_0230

Alternate gene names: 227080461

Gene position: 233917-235308 (Clockwise)

Preceding gene: 227080460

Following gene: 227080462

Centisome position: 8.09

GC content: 38.65

Gene sequence:

>1392_bases
GTGAAAGAGTTAACTTGTTTTAAAGCCTACGATATTCGCGGACAATTAGGGAGTGAGCTTGATAATGAAATTGCCTACAG
AATTGGTCGCTCGTATGGGCAGTTTTTAAAAAGCGAAAATGACGCAGATAAAACAGTTGTGGTAGGTGGTGATGTTCGTT
TAACCAGCGAAGCGCTTAAACAAGCACTAGCTAATGGCTTAATGGATGCAGGTATTAACGTTATTGATATTGGCGTTACT
GGTACCGAAGAGATCTATTTTGCAACCTTCTACCTTGGTGTTGATGGTGGTATTGAAGTTACCGCAAGTCATAACCCAAT
GGATTACAATGGTATGAAGCTGGTTCGTGAAGGTTCAAAACCCATTAGTGGTGATACTGGTTTACGAGAAATTCAAGCTT
TGGCTGAAAAGAATGAATTTATGGACGTTGAAGTTAAAGGTAACTACAAAAAGGTTTCTTTACTTCCAGAATATGTAGAT
CACTTAATATCTTATATTACACCCGCGAAAATTAAACCAATGAAGTTGGTCATCAATTCAGGTAACGGAGCTGCAGGCCA
CGTGATTGATGAGTTGGAAAAACGATTTATTGAATTGAGTATCCCGCTAGAAATTATCAAAGTGCATCATGAAGAAGATG
GTAACTTCCCGAATGGTATTCCTAACCCATTGTTACCTGAATGCCGTGCTGATACCGCAAACGCAGTAAAGGAGCACAAG
GCTGATATGGGCATTGCCTTTGATGGTGACTTTGATCGCTGCTTTTTGTTTGATGAAAATGGCGATTTTATTGAAGGTTA
CTACATCGTAGGTTTATTGGCAGAAGCGTTTTTACAGAAAGAGCAAGGTGCCAAAATTATTCATGATCCACGCTTAAGCT
GGAACACCATTGATGTGGTGACTAAGTCGGGTGGCGTGCCTGTAATGTCTAAAACAGGGCATGCATTTATAAAAGAGCGT
ATGCGCAAAGAAGATGCTATCTACGGTGGTGAAATGAGTGCCCATCACTACTTCCGTGATTTCGGTTACTGTGATTCGGG
GATGATTCCGTGGCTTCTGATTACAGAGTTACTTTCCTTAGCCCCGGATATTAGCTTATCTAAGCTGATATCTGCAAAAA
GATTTTTGTTTCCATGTAGCGGTGAAATTAATTTTAAGGTCAAGCAGGCAAAGCTAATAATGGAGCAAGTTTATTTACAT
TATTACGAGAATTCTATACATTTCTCTGCCATTGATGGTATCTCTCTTGAATTTGAAGGATGGCGTTTTAATCTTAGAGA
TTCAAATACTGAGCCCTTATTAAGATTGAATGTGGAATCTAAACAAAATATCGCGTTGATGAATGATAAAGTTGAAGAGC
TTACTAAATTAATTAAGAAATTGGATATTTAA

Upstream 100 bases:

>100_bases
TGATAGAAAACCCAGGTAAGATCCCGCTAGAGCTTATTGAAATTCAATCCGGTTCATACCTAAACGAAGATGATGTAGTA
CGTTTCGAGGATAAATAAGA

Downstream 100 bases:

>100_bases
CAATGAATAAAAAAGTTGCGTTAATCACAGGCATTACTGGACAAGATGGTTCTTATCTAGCGGAGTTTTTGCTAGAAAAA
GGGTATGAAGTACATGGAAT

Product: phosphomannomutase

Products: NA

Alternate protein names: PMM

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MKELTCFKAYDIRGQLGSELDNEIAYRIGRSYGQFLKSENDADKTVVVGGDVRLTSEALKQALANGLMDAGINVIDIGVT
GTEEIYFATFYLGVDGGIEVTASHNPMDYNGMKLVREGSKPISGDTGLREIQALAEKNEFMDVEVKGNYKKVSLLPEYVD
HLISYITPAKIKPMKLVINSGNGAAGHVIDELEKRFIELSIPLEIIKVHHEEDGNFPNGIPNPLLPECRADTANAVKEHK
ADMGIAFDGDFDRCFLFDENGDFIEGYYIVGLLAEAFLQKEQGAKIIHDPRLSWNTIDVVTKSGGVPVMSKTGHAFIKER
MRKEDAIYGGEMSAHHYFRDFGYCDSGMIPWLLITELLSLAPDISLSKLISAKRFLFPCSGEINFKVKQAKLIMEQVYLH
YYENSIHFSAIDGISLEFEGWRFNLRDSNTEPLLRLNVESKQNIALMNDKVEELTKLIKKLDI

Sequences:

>Translated_463_residues
MKELTCFKAYDIRGQLGSELDNEIAYRIGRSYGQFLKSENDADKTVVVGGDVRLTSEALKQALANGLMDAGINVIDIGVT
GTEEIYFATFYLGVDGGIEVTASHNPMDYNGMKLVREGSKPISGDTGLREIQALAEKNEFMDVEVKGNYKKVSLLPEYVD
HLISYITPAKIKPMKLVINSGNGAAGHVIDELEKRFIELSIPLEIIKVHHEEDGNFPNGIPNPLLPECRADTANAVKEHK
ADMGIAFDGDFDRCFLFDENGDFIEGYYIVGLLAEAFLQKEQGAKIIHDPRLSWNTIDVVTKSGGVPVMSKTGHAFIKER
MRKEDAIYGGEMSAHHYFRDFGYCDSGMIPWLLITELLSLAPDISLSKLISAKRFLFPCSGEINFKVKQAKLIMEQVYLH
YYENSIHFSAIDGISLEFEGWRFNLRDSNTEPLLRLNVESKQNIALMNDKVEELTKLIKKLDI
>Mature_463_residues
MKELTCFKAYDIRGQLGSELDNEIAYRIGRSYGQFLKSENDADKTVVVGGDVRLTSEALKQALANGLMDAGINVIDIGVT
GTEEIYFATFYLGVDGGIEVTASHNPMDYNGMKLVREGSKPISGDTGLREIQALAEKNEFMDVEVKGNYKKVSLLPEYVD
HLISYITPAKIKPMKLVINSGNGAAGHVIDELEKRFIELSIPLEIIKVHHEEDGNFPNGIPNPLLPECRADTANAVKEHK
ADMGIAFDGDFDRCFLFDENGDFIEGYYIVGLLAEAFLQKEQGAKIIHDPRLSWNTIDVVTKSGGVPVMSKTGHAFIKER
MRKEDAIYGGEMSAHHYFRDFGYCDSGMIPWLLITELLSLAPDISLSKLISAKRFLFPCSGEINFKVKQAKLIMEQVYLH
YYENSIHFSAIDGISLEFEGWRFNLRDSNTEPLLRLNVESKQNIALMNDKVEELTKLIKKLDI

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides

COG id: COG1109

COG function: function code G; Phosphomannomutase

Gene ontology:

Cell location: Cell membrane (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphohexose mutase family

Homologues:

Organism=Escherichia coli, GI1788361, Length=462, Percent_Identity=67.3160173160173, Blast_Score=649, Evalue=0.0,
Organism=Escherichia coli, GI1789566, Length=448, Percent_Identity=24.3303571428571, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFBB_VIBCH (Q06951)

Other databases:

- EMBL:   X59554
- EMBL:   AE003852
- PIR:   S28469
- RefSeq:   NP_229899.1
- ProteinModelPortal:   Q06951
- SMR:   Q06951
- GeneID:   2614705
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0242
- TIGR:   VC_0242
- HOGENOM:   HBG644964
- OMA:   DGSWFNL
- ProtClustDB:   CLSK873915
- BRENDA:   5.4.2.8
- InterPro:   IPR005844
- InterPro:   IPR016055
- InterPro:   IPR005845
- InterPro:   IPR005846
- InterPro:   IPR005843
- InterPro:   IPR016066
- InterPro:   IPR005841
- Gene3D:   G3DSA:3.40.120.10
- PRINTS:   PR00509

Pfam domain/function: PF02878 PGM_PMM_I; PF02879 PGM_PMM_II; PF02880 PGM_PMM_III; PF00408 PGM_PMM_IV; SSF53738 A-D-PHexomutase_a/b/a-I/II/III

EC number: =5.4.2.8

Molecular weight: Translated: 51839; Mature: 51839

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00710 PGM_PMM

Important sites: ACT_SITE 103-103

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELTCFKAYDIRGQLGSELDNEIAYRIGRSYGQFLKSENDADKTVVVGGDVRLTSEALK
CCCCEEEEEEECCCHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEHHHHHH
QALANGLMDAGINVIDIGVTGTEEIYFATFYLGVDGGIEVTASHNPMDYNGMKLVREGSK
HHHHHHHHHCCCCEEEECCCCCCCEEEEEEEEECCCCEEEEECCCCCCCCCHHHEECCCC
PISGDTGLREIQALAEKNEFMDVEVKGNYKKVSLLPEYVDHLISYITPAKIKPMKLVINS
CCCCCCHHHHHHHHHHCCCEEEEEEECCEEEEEHHHHHHHHHHHHCCCCCCCEEEEEEEC
GNGAAGHVIDELEKRFIELSIPLEIIKVHHEEDGNFPNGIPNPLLPECRADTANAVKEHK
CCCCCHHHHHHHHHHHEEEECCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
ADMGIAFDGDFDRCFLFDENGDFIEGYYIVGLLAEAFLQKEQGAKIIHDPRLSWNTIDVV
CCCCEEECCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEEEE
TKSGGVPVMSKTGHAFIKERMRKEDAIYGGEMSAHHYFRDFGYCDSGMIPWLLITELLSL
ECCCCCCEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHH
APDISLSKLISAKRFLFPCSGEINFKVKQAKLIMEQVYLHYYENSIHFSAIDGISLEFEG
CCCCCHHHHHHHHHEEEECCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEEECCEEEEECC
WRFNLRDSNTEPLLRLNVESKQNIALMNDKVEELTKLIKKLDI
EEEEECCCCCCCEEEEECCCCCCEEEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKELTCFKAYDIRGQLGSELDNEIAYRIGRSYGQFLKSENDADKTVVVGGDVRLTSEALK
CCCCEEEEEEECCCHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEHHHHHH
QALANGLMDAGINVIDIGVTGTEEIYFATFYLGVDGGIEVTASHNPMDYNGMKLVREGSK
HHHHHHHHHCCCCEEEECCCCCCCEEEEEEEEECCCCEEEEECCCCCCCCCHHHEECCCC
PISGDTGLREIQALAEKNEFMDVEVKGNYKKVSLLPEYVDHLISYITPAKIKPMKLVINS
CCCCCCHHHHHHHHHHCCCEEEEEEECCEEEEEHHHHHHHHHHHHCCCCCCCEEEEEEEC
GNGAAGHVIDELEKRFIELSIPLEIIKVHHEEDGNFPNGIPNPLLPECRADTANAVKEHK
CCCCCHHHHHHHHHHHEEEECCEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
ADMGIAFDGDFDRCFLFDENGDFIEGYYIVGLLAEAFLQKEQGAKIIHDPRLSWNTIDVV
CCCCEEECCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEEEE
TKSGGVPVMSKTGHAFIKERMRKEDAIYGGEMSAHHYFRDFGYCDSGMIPWLLITELLSL
ECCCCCCEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHH
APDISLSKLISAKRFLFPCSGEINFKVKQAKLIMEQVYLHYYENSIHFSAIDGISLEFEG
CCCCCHHHHHHHHHEEEECCCCEEEEEHHHHHHHHHHHHHHHCCCEEEEEECCEEEEECC
WRFNLRDSNTEPLLRLNVESKQNIALMNDKVEELTKLIKKLDI
EEEEECCCCCCCEEEEECCCCCCEEEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1372980; 10952301