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The map label for this gene is rfbA

Identifier: 227080460

GI number: 227080460

Start: 232517

End: 233914

Strand: Direct

Name: rfbA

Synonym: VCM66_0229

Alternate gene names: 227080460

Gene position: 232517-233914 (Clockwise)

Preceding gene: 227080448

Following gene: 227080461

Centisome position: 8.04

GC content: 42.13

Gene sequence:

>1398_bases
ATGTTTATACCTGTAATTATGGCTGGTGGCTCCGGCTCTAGGCTTTGGCCCCTTTCACGTTCGGCTTTTCCAAAACAATT
CTTATCCTTAGATAGCAGTAGTCAGCATACTATGCTACAAGCCACTATTGAGCGTTTACAGGGGCTACCTATTGCTGAAC
CCATTGTTATTTCGAATGAAGATCATCGTTTTATCGTTGCGGAACAAATTCGTCGTTATGGCAAAAAGTCTCGCATTATC
TTAGAACCAGCAGGAAGGAACACTGCACCTGCGATCGCCTTAGCAGCATTTACGGCAATCGAACAGGAGGATGACCCCGT
ATTGCTAGTATTGGCTGCCGATCATTTTGTAAAAAATAAAAGTGCATTTCAGGCTGCTATTTCACAAGCAGCTCAACAGG
CAGAGGCTGGTAAACTTGCTACTTTTGGTATCGTTCCCACCACTCCTGAAACAGGTTATGGTTATATTCACCGGGGTGAA
GAGGTTACACAAGGCACTTATGAAATTAACTCATTTGTTGAAAAGCCACAGTTAAATATTGCTGAGCAGTATTTAGCCAG
TGGTGAATATTACTGGAACAGTGGTTGTTTTATGTTTAAAGCTTCCGTGTTCTTAAATGAGTTGAAGCAACATTCTCCAG
AGATCTATCGCCAATGTGAACTTGCAATGCAAGGCCTTTCTCACGATTACGATTTTATTCGTGTCGGTGTAGAAGAGTTC
CTTAAATGTCCTGATGATTCAATCGACTATGCCGTTATGGAGCACACTAAACTAGGTGTGGTAGTATCTATGGATGCCGG
ATGGAGTGATGTAGGTTCTTGGTCTGCATTATGGGAAGTATCTGATAAAGATGCAGACGGTAACGTATGCCAAGGTGATG
CCATTTTAAGTGGCACCTCTAATTGTTATATCTACGCCCCAAATAAACTGGTTGCTGCGGTTGGCTTGAAAGATATTGTT
GTGGTGGAAACTAAAGATGCCGTGTTAGTTGCAGACAAAAACCAAGTACAAGAAGTCAAGAAGATAGTTGAACATTTGAA
GGCAGAAAACCGAGCTGAATATCGAGAACACAGGGAACGTTATCGCCCTTGGGGTAAATCTGATGCGATTGATAAAGGCG
AGCGTTACAAAGTAAACCGTATTACGGTTGAGCCTGGTAAAAAGCAATCATTGCAGATGCATTACCACCGAGCAGAGCAC
TGGGTAGTGGTCTCTGGTACTGCCAAGGTAACTTGTGAAGGTAATGTTAAAGTAATCACGGAAAACCAGTCCTTGTATAT
CCCTATTGGTACAAATCATATGATAGAAAACCCAGGTAAGATCCCGCTAGAGCTTATTGAAATTCAATCCGGTTCATACC
TAAACGAAGATGATGTAGTACGTTTCGAGGATAAATAA

Upstream 100 bases:

>100_bases
GTATTTCCATTTCTATCAATGGTTTATCAGCTTTTCAACAATGCTCTAATCTAGGTTTGAGAACAAATAAAATCATAAAA
CTAATTGTTGTGAGAGAAAG

Downstream 100 bases:

>100_bases
GAGTGAAAGAGTTAACTTGTTTTAAAGCCTACGATATTCGCGGACAATTAGGGAGTGAGCTTGATAATGAAATTGCCTAC
AGAATTGGTCGCTCGTATGG

Product: mannose-1-phosphate guanylyltransferase

Products: NA

Alternate protein names: GDP-mannose pyrophosphorylase; GMP; GMPP

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MFIPVIMAGGSGSRLWPLSRSAFPKQFLSLDSSSQHTMLQATIERLQGLPIAEPIVISNEDHRFIVAEQIRRYGKKSRII
LEPAGRNTAPAIALAAFTAIEQEDDPVLLVLAADHFVKNKSAFQAAISQAAQQAEAGKLATFGIVPTTPETGYGYIHRGE
EVTQGTYEINSFVEKPQLNIAEQYLASGEYYWNSGCFMFKASVFLNELKQHSPEIYRQCELAMQGLSHDYDFIRVGVEEF
LKCPDDSIDYAVMEHTKLGVVVSMDAGWSDVGSWSALWEVSDKDADGNVCQGDAILSGTSNCYIYAPNKLVAAVGLKDIV
VVETKDAVLVADKNQVQEVKKIVEHLKAENRAEYREHRERYRPWGKSDAIDKGERYKVNRITVEPGKKQSLQMHYHRAEH
WVVVSGTAKVTCEGNVKVITENQSLYIPIGTNHMIENPGKIPLELIEIQSGSYLNEDDVVRFEDK

Sequences:

>Translated_465_residues
MFIPVIMAGGSGSRLWPLSRSAFPKQFLSLDSSSQHTMLQATIERLQGLPIAEPIVISNEDHRFIVAEQIRRYGKKSRII
LEPAGRNTAPAIALAAFTAIEQEDDPVLLVLAADHFVKNKSAFQAAISQAAQQAEAGKLATFGIVPTTPETGYGYIHRGE
EVTQGTYEINSFVEKPQLNIAEQYLASGEYYWNSGCFMFKASVFLNELKQHSPEIYRQCELAMQGLSHDYDFIRVGVEEF
LKCPDDSIDYAVMEHTKLGVVVSMDAGWSDVGSWSALWEVSDKDADGNVCQGDAILSGTSNCYIYAPNKLVAAVGLKDIV
VVETKDAVLVADKNQVQEVKKIVEHLKAENRAEYREHRERYRPWGKSDAIDKGERYKVNRITVEPGKKQSLQMHYHRAEH
WVVVSGTAKVTCEGNVKVITENQSLYIPIGTNHMIENPGKIPLELIEIQSGSYLNEDDVVRFEDK
>Mature_465_residues
MFIPVIMAGGSGSRLWPLSRSAFPKQFLSLDSSSQHTMLQATIERLQGLPIAEPIVISNEDHRFIVAEQIRRYGKKSRII
LEPAGRNTAPAIALAAFTAIEQEDDPVLLVLAADHFVKNKSAFQAAISQAAQQAEAGKLATFGIVPTTPETGYGYIHRGE
EVTQGTYEINSFVEKPQLNIAEQYLASGEYYWNSGCFMFKASVFLNELKQHSPEIYRQCELAMQGLSHDYDFIRVGVEEF
LKCPDDSIDYAVMEHTKLGVVVSMDAGWSDVGSWSALWEVSDKDADGNVCQGDAILSGTSNCYIYAPNKLVAAVGLKDIV
VVETKDAVLVADKNQVQEVKKIVEHLKAENRAEYREHRERYRPWGKSDAIDKGERYKVNRITVEPGKKQSLQMHYHRAEH
WVVVSGTAKVTCEGNVKVITENQSLYIPIGTNHMIENPGKIPLELIEIQSGSYLNEDDVVRFEDK

Specific function: Involved In The Biosynthesis Of The Capsular Polysaccharide Colanic Acid. [C]

COG id: COG0836

COG function: function code M; Mannose-1-phosphate guanylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 2 family

Homologues:

Organism=Escherichia coli, GI1788362, Length=470, Percent_Identity=62.1276595744681, Blast_Score=581, Evalue=1e-167,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFBA_VIBCH (Q07024)

Other databases:

- EMBL:   X59554
- EMBL:   AE003852
- PIR:   H82345
- PIR:   S28468
- RefSeq:   NP_229898.1
- ProteinModelPortal:   Q07024
- SMR:   Q07024
- GeneID:   2614704
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0241
- TIGR:   VC_0241
- HOGENOM:   HBG422488
- OMA:   AEHRMVA
- ProtClustDB:   CLSK793534
- BRENDA:   2.7.7.22
- InterPro:   IPR011051
- InterPro:   IPR006375
- InterPro:   IPR001538
- InterPro:   IPR005835
- InterPro:   IPR014710
- Gene3D:   G3DSA:2.60.120.10
- TIGRFAMs:   TIGR01479

Pfam domain/function: PF01050 MannoseP_isomer; PF00483 NTP_transferase; SSF51182 RmlC_like_cupin

EC number: =2.7.7.13

Molecular weight: Translated: 51916; Mature: 51916

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFIPVIMAGGSGSRLWPLSRSAFPKQFLSLDSSSQHTMLQATIERLQGLPIAEPIVISNE
CEEEEEEECCCCCEEECCCCCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECC
DHRFIVAEQIRRYGKKSRIILEPAGRNTAPAIALAAFTAIEQEDDPVLLVLAADHFVKNK
CCEEEHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCHHHCCH
SAFQAAISQAAQQAEAGKLATFGIVPTTPETGYGYIHRGEEVTQGTYEINSFVEKPQLNI
HHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEECCCHHCCCHHHHHHHHCCCCCHH
AEQYLASGEYYWNSGCFMFKASVFLNELKQHSPEIYRQCELAMQGLSHDYDFIRVGVEEF
HHHHHHCCCEEECCCEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHCCHHHH
LKCPDDSIDYAVMEHTKLGVVVSMDAGWSDVGSWSALWEVSDKDADGNVCQGDAILSGTS
HCCCCCCCCEEEEECCEEEEEEEECCCCCCCCCCEEEEEECCCCCCCCEEECCEEEECCC
NCYIYAPNKLVAAVGLKDIVVVETKDAVLVADKNQVQEVKKIVEHLKAENRAEYREHRER
CEEEECCCCEEEECCCCEEEEEEECCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHH
YRPWGKSDAIDKGERYKVNRITVEPGKKQSLQMHYHRAEHWVVVSGTAKVTCEGNVKVIT
HCCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEEEECCCEEEEEECCEEEEECCCEEEEE
ENQSLYIPIGTNHMIENPGKIPLELIEIQSGSYLNEDDVVRFEDK
ECCEEEEEECCCCEECCCCCCCEEEEEECCCCCCCCCCEEEECCC
>Mature Secondary Structure
MFIPVIMAGGSGSRLWPLSRSAFPKQFLSLDSSSQHTMLQATIERLQGLPIAEPIVISNE
CEEEEEEECCCCCEEECCCCCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCEEEECC
DHRFIVAEQIRRYGKKSRIILEPAGRNTAPAIALAAFTAIEQEDDPVLLVLAADHFVKNK
CCEEEHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCHHHCCH
SAFQAAISQAAQQAEAGKLATFGIVPTTPETGYGYIHRGEEVTQGTYEINSFVEKPQLNI
HHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEECCCHHCCCHHHHHHHHCCCCCHH
AEQYLASGEYYWNSGCFMFKASVFLNELKQHSPEIYRQCELAMQGLSHDYDFIRVGVEEF
HHHHHHCCCEEECCCEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHCCHHHH
LKCPDDSIDYAVMEHTKLGVVVSMDAGWSDVGSWSALWEVSDKDADGNVCQGDAILSGTS
HCCCCCCCCEEEEECCEEEEEEEECCCCCCCCCCEEEEEECCCCCCCCEEECCEEEECCC
NCYIYAPNKLVAAVGLKDIVVVETKDAVLVADKNQVQEVKKIVEHLKAENRAEYREHRER
CEEEECCCCEEEECCCCEEEEEEECCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHH
YRPWGKSDAIDKGERYKVNRITVEPGKKQSLQMHYHRAEHWVVVSGTAKVTCEGNVKVIT
HCCCCCCCCCCCCCCEEEEEEEECCCCCCCCEEEEECCCEEEEEECCEEEEECCCEEEEE
ENQSLYIPIGTNHMIENPGKIPLELIEIQSGSYLNEDDVVRFEDK
ECCEEEEEECCCCEECCCCCCCEEEEEECCCCCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1372980; 10952301