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The map label for this gene is mutM

Identifier: 227080441

GI number: 227080441

Start: 213355

End: 214164

Strand: Direct

Name: mutM

Synonym: VCM66_0209

Alternate gene names: 227080441

Gene position: 213355-214164 (Clockwise)

Preceding gene: 227080440

Following gene: 227080443

Centisome position: 7.38

GC content: 52.22

Gene sequence:

>810_bases
ATGCCTGAATTACCTGAAGTTGAAGTCAGCCGTTTAGGGATCTCCCCACATTTGGTCGGTGGCACCATTCAATCCTTAGT
GTTACGTACGCCCAAGTTGCGCTGGCCTATCCCTCAAGAGCTGAAACAGCTTGAAGGGCAGACCATTCTCGCGATTCATC
GTCGAGCCAAGTATCTGATTATTGAAACCGCCGTCGGCAGTGCGATTGTGCATTTGGGTATGTCTGGCTCGCTGCGTATT
TTGGATGGCGATTTTCCTGCCGCGAAACACGATCATGTGGATCTGGTGATGACCAGCGGCAAACGGCTGCGCTATAACGA
TCCGCGTCGCTTCGGGGCTTGGTTATGGTGTGCGCCTGATGAGAGCCATGAAGTGCTCGGGCGTTTGGGGCCTGAGCCGT
TGACCGAAGCTTTTAACGCTGAATATATGATGGACAAAGCGCGCAACAAACGCATTGCAGTCAAAGCTTTTATCATGGAC
AACGCAGCGGTCGTGGGTGTGGGGAATATCTATGCTAATGAATCGCTGTTTACATCACGCCTCCATCCACTACGCCCCGC
TCATTCGCTGAGCTTAGAGGAGTGGCAAACCTTAGTGGCGAACATCAAACAAGTGCTGCAAGTGGCGATTAAGCAAGGCG
GCACCACACTGAAAGATTTTACCCAAAGCGATGGTAAGCCGGGCTATTTCGCGCAAGAGCTGCAAGTGTATGGCAAAGCC
AAACAGCCTTGCCCCCATTGTGGAGAGCCGCTTTGCGAGCAGAAAATTGCTCAGCGCAATACCTTTTTCTGCCCGCAGTG
CCAGCATTGA

Upstream 100 bases:

>100_bases
AACCCCACAATTTTGGTTGCTAAAAAACTGGCAAGATCGTTGGATTGCGGTCTCGGCCGAAGCGAGTTATCTATTCCCTG
CGCCATTGTAAGAGGTTGTC

Downstream 100 bases:

>100_bases
TATACCCAAACGACTTAGAGTTGCAGGTAGCCAACATCGCTGCAGCTTCAAGTAGGAAGGGGATTGAGGGATGTGAGCTT
GCATCCCTTTGAAGCGCTAG

Product: formamidopyrimidine-DNA glycosylase

Products: NA

Alternate protein names: Fapy-DNA glycosylase; DNA-(apurinic or apyrimidinic site) lyase mutM; AP lyase mutM

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MPELPEVEVSRLGISPHLVGGTIQSLVLRTPKLRWPIPQELKQLEGQTILAIHRRAKYLIIETAVGSAIVHLGMSGSLRI
LDGDFPAAKHDHVDLVMTSGKRLRYNDPRRFGAWLWCAPDESHEVLGRLGPEPLTEAFNAEYMMDKARNKRIAVKAFIMD
NAAVVGVGNIYANESLFTSRLHPLRPAHSLSLEEWQTLVANIKQVLQVAIKQGGTTLKDFTQSDGKPGYFAQELQVYGKA
KQPCPHCGEPLCEQKIAQRNTFFCPQCQH

Sequences:

>Translated_269_residues
MPELPEVEVSRLGISPHLVGGTIQSLVLRTPKLRWPIPQELKQLEGQTILAIHRRAKYLIIETAVGSAIVHLGMSGSLRI
LDGDFPAAKHDHVDLVMTSGKRLRYNDPRRFGAWLWCAPDESHEVLGRLGPEPLTEAFNAEYMMDKARNKRIAVKAFIMD
NAAVVGVGNIYANESLFTSRLHPLRPAHSLSLEEWQTLVANIKQVLQVAIKQGGTTLKDFTQSDGKPGYFAQELQVYGKA
KQPCPHCGEPLCEQKIAQRNTFFCPQCQH
>Mature_268_residues
PELPEVEVSRLGISPHLVGGTIQSLVLRTPKLRWPIPQELKQLEGQTILAIHRRAKYLIIETAVGSAIVHLGMSGSLRIL
DGDFPAAKHDHVDLVMTSGKRLRYNDPRRFGAWLWCAPDESHEVLGRLGPEPLTEAFNAEYMMDKARNKRIAVKAFIMDN
AAVVGVGNIYANESLFTSRLHPLRPAHSLSLEEWQTLVANIKQVLQVAIKQGGTTLKDFTQSDGKPGYFAQELQVYGKAK
QPCPHCGEPLCEQKIAQRNTFFCPQCQH

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyr

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger

Homologues:

Organism=Escherichia coli, GI1790066, Length=268, Percent_Identity=61.1940298507463, Blast_Score=337, Evalue=4e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FPG_VIBC3 (A5F407)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F407
- SMR:   A5F407
- STRING:   A5F407
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2602
- eggNOG:   COG0266
- HOGENOM:   HBG690070
- OMA:   RMTGQLL
- ProtClustDB:   PRK01103
- BioCyc:   VCHO345073:VC0395_A2602-MONOMER
- HAMAP:   MF_00103
- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR000191
- InterPro:   IPR012319
- InterPro:   IPR020629
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663
- SMART:   SM00898
- TIGRFAMs:   TIGR00577

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS; SSF81624 Form_DNAglyc_cat; SSF46946 Ribosomal_H2TH

EC number: =3.2.2.23; =4.2.99.18

Molecular weight: Translated: 30037; Mature: 29906

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS51068 FPG_CAT; PS01242 ZF_FPG_1; PS51066 ZF_FPG_2

Important sites: ACT_SITE 2-2 ACT_SITE 3-3 ACT_SITE 57-57 ACT_SITE 259-259 BINDING 90-90 BINDING 109-109 BINDING 150-150

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELPEVEVSRLGISPHLVGGTIQSLVLRTPKLRWPIPQELKQLEGQTILAIHRRAKYLI
CCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEEEECCCEEEE
IETAVGSAIVHLGMSGSLRILDGDFPAAKHDHVDLVMTSGKRLRYNDPRRFGAWLWCAPD
EEEHHHHHHHEECCCCCEEEEECCCCCCCCCCEEEEEECCCEEECCCCHHHCEEEEECCC
ESHEVLGRLGPEPLTEAFNAEYMMDKARNKRIAVKAFIMDNAAVVGVGNIYANESLFTSR
CHHHHHHHCCCHHHHHHHCHHHHHHHHCCCEEEEEEEEECCCEEEEECCHHCCCHHHHHH
LHPLRPAHSLSLEEWQTLVANIKQVLQVAIKQGGTTLKDFTQSDGKPGYFAQELQVYGKA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHCCC
KQPCPHCGEPLCEQKIAQRNTFFCPQCQH
CCCCCCCCCHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PELPEVEVSRLGISPHLVGGTIQSLVLRTPKLRWPIPQELKQLEGQTILAIHRRAKYLI
CCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEEEEECCCEEEE
IETAVGSAIVHLGMSGSLRILDGDFPAAKHDHVDLVMTSGKRLRYNDPRRFGAWLWCAPD
EEEHHHHHHHEECCCCCEEEEECCCCCCCCCCEEEEEECCCEEECCCCHHHCEEEEECCC
ESHEVLGRLGPEPLTEAFNAEYMMDKARNKRIAVKAFIMDNAAVVGVGNIYANESLFTSR
CHHHHHHHCCCHHHHHHHCHHHHHHHHCCCEEEEEEEEECCCEEEEECCHHCCCHHHHHH
LHPLRPAHSLSLEEWQTLVANIKQVLQVAIKQGGTTLKDFTQSDGKPGYFAQELQVYGKA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHCCC
KQPCPHCGEPLCEQKIAQRNTFFCPQCQH
CCCCCCCCCHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA