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The map label for this gene is rpoH

Identifier: 227080385

GI number: 227080385

Start: 139975

End: 140835

Strand: Direct

Name: rpoH

Synonym: VCM66_0150

Alternate gene names: 227080385

Gene position: 139975-140835 (Clockwise)

Preceding gene: 227080384

Following gene: 227080387

Centisome position: 4.84

GC content: 49.94

Gene sequence:

>861_bases
ATGACTAACCAAGCGTATCCAATGGCTCTTGTTTCCCAAGACAGCTTAGATAGCTACATCCGTTCAGTAAACGGTTACCC
GATGCTGAGTGCTGATGAAGAGCGCGAGCTGGCAGAGCGATTACATTACAAAGGGGATATCGATGCTGCGAAAGGCTTGA
TCTTGTCGCACCTACGATTTGTTGTTCACGTTGCTCGTGGTTATTCCGGTTATGGCTTGCCAATGGCGGACTTAGTGCAA
GAGGGCAATATCGGTCTGATGAAAGCGGTTAAACGCTTTAACCCTGAGATGGGGGTAAGACTGGTGTCGTTTGCGGTGCA
CTGGATTAAAGCCGAAATTCATGAATATGTGCTACGTAACTGGCGTATTGTGAAAATCGCCACCACCAAAGCACAGCGCA
AACTGTTCTTTAATCTGCGTAAATCGAAAAAACGCCTTGGCTGGTTTAATAACGGCGAAGTCGAAACGGTAGCGCGCGAG
CTGGGTGTTGAGCCTGCTGAAGTGCGTGAAATGGAATCTCGTCTGGCAGCCCAAGATGCTGCGTTTGAGATGTCCGCCGA
GGATGACGAAAACGGCATGGCCTACACTGCGCCTGTGCTGTACCTCGAAGATAAGCACTCTGACTTAGCTGATAACCTAG
AAGCCGAAAACTGGGAAGCGCATACCACACAGCGCCTCAGCATGGCGCTGGCAAGTCTTGATGAGCGTAGCCAACACATT
GTGCGTGCTCGTTGGTTGGATGATGACAACAAAACCACACTGCAAGATTTGGCGGAAATGTATGGTGTTTCTGCGGAGCG
TATTCGTCAGCTTGAGAAAAACGCCATGCGTAAGCTGAAAGAAGCGGTGGGCGAGTTCTGA

Upstream 100 bases:

>100_bases
TTATGCATAATGACAAGTCAAACTTGAAACCTGTTCATTTTAGGTTCAAGCTTGTTGCGGTAAAACAGCGTATCCAGATC
AGAGAATGATGAGGAATTGA

Downstream 100 bases:

>100_bases
TTTCACATTGCGTAACAGCTTGATAAGAAAAGGTCTGGTCAATGCCAGACCTTTTTTGTCTCTATCTATATACCCTTCCT
ACTTGAAGCTGCAGCGGTGT

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MTNQAYPMALVSQDSLDSYIRSVNGYPMLSADEERELAERLHYKGDIDAAKGLILSHLRFVVHVARGYSGYGLPMADLVQ
EGNIGLMKAVKRFNPEMGVRLVSFAVHWIKAEIHEYVLRNWRIVKIATTKAQRKLFFNLRKSKKRLGWFNNGEVETVARE
LGVEPAEVREMESRLAAQDAAFEMSAEDDENGMAYTAPVLYLEDKHSDLADNLEAENWEAHTTQRLSMALASLDERSQHI
VRARWLDDDNKTTLQDLAEMYGVSAERIRQLEKNAMRKLKEAVGEF

Sequences:

>Translated_286_residues
MTNQAYPMALVSQDSLDSYIRSVNGYPMLSADEERELAERLHYKGDIDAAKGLILSHLRFVVHVARGYSGYGLPMADLVQ
EGNIGLMKAVKRFNPEMGVRLVSFAVHWIKAEIHEYVLRNWRIVKIATTKAQRKLFFNLRKSKKRLGWFNNGEVETVARE
LGVEPAEVREMESRLAAQDAAFEMSAEDDENGMAYTAPVLYLEDKHSDLADNLEAENWEAHTTQRLSMALASLDERSQHI
VRARWLDDDNKTTLQDLAEMYGVSAERIRQLEKNAMRKLKEAVGEF
>Mature_285_residues
TNQAYPMALVSQDSLDSYIRSVNGYPMLSADEERELAERLHYKGDIDAAKGLILSHLRFVVHVARGYSGYGLPMADLVQE
GNIGLMKAVKRFNPEMGVRLVSFAVHWIKAEIHEYVLRNWRIVKIATTKAQRKLFFNLRKSKKRLGWFNNGEVETVAREL
GVEPAEVREMESRLAAQDAAFEMSAEDDENGMAYTAPVLYLEDKHSDLADNLEAENWEAHTTQRLSMALASLDERSQHIV
RARWLDDDNKTTLQDLAEMYGVSAERIRQLEKNAMRKLKEAVGEF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily

Homologues:

Organism=Escherichia coli, GI1789871, Length=283, Percent_Identity=71.0247349823322, Blast_Score=414, Evalue=1e-117,
Organism=Escherichia coli, GI1789098, Length=290, Percent_Identity=26.551724137931, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1789448, Length=241, Percent_Identity=29.8755186721992, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): RP32_VIBCH (P50511)

Other databases:

- EMBL:   U44432
- EMBL:   AE003852
- PIR:   D82357
- RefSeq:   NP_229808.1
- ProteinModelPortal:   P50511
- SMR:   P50511
- GeneID:   2614865
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC0150
- TIGR:   VC_0150
- HOGENOM:   HBG745096
- OMA:   VNSIPVL
- ProtClustDB:   PRK06596
- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00046
- TIGRFAMs:   TIGR02392
- TIGRFAMs:   TIGR02937

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4; SSF88946 Sigma_r2; SSF88659 Sigma_r3_r4

EC number: NA

Molecular weight: Translated: 32651; Mature: 32519

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00715 SIGMA70_1; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNQAYPMALVSQDSLDSYIRSVNGYPMLSADEERELAERLHYKGDIDAAKGLILSHLRF
CCCCCCCCHHHCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VVHVARGYSGYGLPMADLVQEGNIGLMKAVKRFNPEMGVRLVSFAVHWIKAEIHEYVLRN
HHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHC
WRIVKIATTKAQRKLFFNLRKSKKRLGWFNNGEVETVARELGVEPAEVREMESRLAAQDA
CEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
AFEMSAEDDENGMAYTAPVLYLEDKHSDLADNLEAENWEAHTTQRLSMALASLDERSQHI
HHCCCCCCCCCCEEEEEEEEEEECCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VRARWLDDDNKTTLQDLAEMYGVSAERIRQLEKNAMRKLKEAVGEF
HHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TNQAYPMALVSQDSLDSYIRSVNGYPMLSADEERELAERLHYKGDIDAAKGLILSHLRF
CCCCCCCHHHCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHH
VVHVARGYSGYGLPMADLVQEGNIGLMKAVKRFNPEMGVRLVSFAVHWIKAEIHEYVLRN
HHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHC
WRIVKIATTKAQRKLFFNLRKSKKRLGWFNNGEVETVARELGVEPAEVREMESRLAAQDA
CEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
AFEMSAEDDENGMAYTAPVLYLEDKHSDLADNLEAENWEAHTTQRLSMALASLDERSQHI
HHCCCCCCCCCCEEEEEEEEEEECCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VRARWLDDDNKTTLQDLAEMYGVSAERIRQLEKNAMRKLKEAVGEF
HHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9168128; 10952301