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The map label for this gene is hemB [H]

Identifier: 227080341

GI number: 227080341

Start: 100776

End: 101819

Strand: Direct

Name: hemB [H]

Synonym: VCM66_0105

Alternate gene names: 227080341

Gene position: 100776-101819 (Clockwise)

Preceding gene: 227080333

Following gene: 227080342

Centisome position: 3.48

GC content: 49.62

Gene sequence:

>1044_bases
GTGTCGGTATCCATTCAAGGTCAATTTCCTGGGCGTCGTTTACGCCGCCTGCGTAAACATGATTTCAGCCGTCGCCTTGT
GGCCGAAAACCAGTTGTCGGTGAACGATCTGATCTACCCGATGTTTATTTTGATGGGTAAAGATCGCCGTGAAAAAGTCG
ATTCCATGCCGGGCGTAGAACGTTTATCCATCGATTTAATGCTGGAAGAAGCACAGTATCTCGCTAACCTCGGTGTGCCT
GCGATTGCTCTGTTTCCTGTGGTCAATCAGGATGCAAAAAGCCTATGTGCTGCCGAAGCTTACAACCCTGAAGGCTTAGT
TCAACGCGCAGTGCGTGCGCTGAAAGAGCATGTGCCGCAAATGGGCGTGATCACTGATGTGGCATTGGATCCATTCACAA
CTCATGGACAAGACGGCATCATCGATGAGCAAGGTTATGTGTTGAATGATGAAACAACGGAAGTGCTAGTGAAGCAAGCA
CTGTCGCATGCTCAAGCTGGTGCGGATGTGGTTGCCCCTTCCGATATGATGGATGGCCGTATTGGGCGCATTCGTCAAGC
ATTGGAAGAGGCGGGGTACATTCATACTCAAATCATGGCTTATTCCGCTAAATATGCCTCGAATTACTATGGGCCATTCC
GTGATGCGGTCGGGTCATCTGCGAATTTAAAAGGTGGCAACAAGAAAAACTACCAAATGGACCCCGCCAATTCCGATGAA
GCTTTGCATGAAGTCGCGATGGACATCAATGAAGGTGCGGACATGGTGATGGTGAAGCCCGGCATGCCCTATCTAGATGT
GGTGCGCCGTGTCAAAACCGAGTTGCAGGTACCGACCTTCGCTTACCAAGTTTCCGGTGAGTATGCGATGCACAAAGCGG
CAATCATGAATGGTTGGTTGAAAGAGCGGGAAACGGTATTCGAATCTTTACTCTGCTTCAAACGTGCAGGTGCAGATGGC
ATTTTGACTTACTTTGCGAAAGAGGTGGCTGAGTGGTTGGCGGAAGACAGCGCAAAAGCCGCGCAGTTCTTACCTAAAAA
GTAA

Upstream 100 bases:

>100_bases
TTTGCCGCTAGCATGCCGAGCCGAGAGTGATATGATTTTGCCCAGCACTTCCCTCAGCACCTGATTTTTCAGGCACTGAC
CACATAAGAAGGAGAATAGA

Downstream 100 bases:

>100_bases
AGAATACACCGCGCTAATAAAGCAAAGCTCAAGGCTGACGTAAGTCAGCCTTTTTAACATCTCTAAACCACTGTTTTTAG
CGATGGCGATTGTTTGAGGA

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MSVSIQGQFPGRRLRRLRKHDFSRRLVAENQLSVNDLIYPMFILMGKDRREKVDSMPGVERLSIDLMLEEAQYLANLGVP
AIALFPVVNQDAKSLCAAEAYNPEGLVQRAVRALKEHVPQMGVITDVALDPFTTHGQDGIIDEQGYVLNDETTEVLVKQA
LSHAQAGADVVAPSDMMDGRIGRIRQALEEAGYIHTQIMAYSAKYASNYYGPFRDAVGSSANLKGGNKKNYQMDPANSDE
ALHEVAMDINEGADMVMVKPGMPYLDVVRRVKTELQVPTFAYQVSGEYAMHKAAIMNGWLKERETVFESLLCFKRAGADG
ILTYFAKEVAEWLAEDSAKAAQFLPKK

Sequences:

>Translated_347_residues
MSVSIQGQFPGRRLRRLRKHDFSRRLVAENQLSVNDLIYPMFILMGKDRREKVDSMPGVERLSIDLMLEEAQYLANLGVP
AIALFPVVNQDAKSLCAAEAYNPEGLVQRAVRALKEHVPQMGVITDVALDPFTTHGQDGIIDEQGYVLNDETTEVLVKQA
LSHAQAGADVVAPSDMMDGRIGRIRQALEEAGYIHTQIMAYSAKYASNYYGPFRDAVGSSANLKGGNKKNYQMDPANSDE
ALHEVAMDINEGADMVMVKPGMPYLDVVRRVKTELQVPTFAYQVSGEYAMHKAAIMNGWLKERETVFESLLCFKRAGADG
ILTYFAKEVAEWLAEDSAKAAQFLPKK
>Mature_346_residues
SVSIQGQFPGRRLRRLRKHDFSRRLVAENQLSVNDLIYPMFILMGKDRREKVDSMPGVERLSIDLMLEEAQYLANLGVPA
IALFPVVNQDAKSLCAAEAYNPEGLVQRAVRALKEHVPQMGVITDVALDPFTTHGQDGIIDEQGYVLNDETTEVLVKQAL
SHAQAGADVVAPSDMMDGRIGRIRQALEEAGYIHTQIMAYSAKYASNYYGPFRDAVGSSANLKGGNKKNYQMDPANSDEA
LHEVAMDINEGADMVMVKPGMPYLDVVRRVKTELQVPTFAYQVSGEYAMHKAAIMNGWLKERETVFESLLCFKRAGADGI
LTYFAKEVAEWLAEDSAKAAQFLPKK

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=312, Percent_Identity=39.4230769230769, Blast_Score=224, Evalue=9e-59,
Organism=Escherichia coli, GI87081728, Length=320, Percent_Identity=44.375, Blast_Score=253, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6321398, Length=322, Percent_Identity=36.6459627329193, Blast_Score=209, Evalue=4e-55,
Organism=Drosophila melanogaster, GI21358291, Length=316, Percent_Identity=37.6582278481013, Blast_Score=216, Evalue=1e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 38539; Mature: 38407

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSIQGQFPGRRLRRLRKHDFSRRLVAENQLSVNDLIYPMFILMGKDRREKVDSMPGVE
CCEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCHH
RLSIDLMLEEAQYLANLGVPAIALFPVVNQDAKSLCAAEAYNPEGLVQRAVRALKEHVPQ
HHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
MGVITDVALDPFTTHGQDGIIDEQGYVLNDETTEVLVKQALSHAQAGADVVAPSDMMDGR
CCCHHHHCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCH
IGRIRQALEEAGYIHTQIMAYSAKYASNYYGPFRDAVGSSANLKGGNKKNYQMDPANSDE
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCHH
ALHEVAMDINEGADMVMVKPGMPYLDVVRRVKTELQVPTFAYQVSGEYAMHKAAIMNGWL
HHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHH
KERETVFESLLCFKRAGADGILTYFAKEVAEWLAEDSAKAAQFLPKK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHCCCCC
>Mature Secondary Structure 
SVSIQGQFPGRRLRRLRKHDFSRRLVAENQLSVNDLIYPMFILMGKDRREKVDSMPGVE
CEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCHH
RLSIDLMLEEAQYLANLGVPAIALFPVVNQDAKSLCAAEAYNPEGLVQRAVRALKEHVPQ
HHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
MGVITDVALDPFTTHGQDGIIDEQGYVLNDETTEVLVKQALSHAQAGADVVAPSDMMDGR
CCCHHHHCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCCHHHHCCH
IGRIRQALEEAGYIHTQIMAYSAKYASNYYGPFRDAVGSSANLKGGNKKNYQMDPANSDE
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCHH
ALHEVAMDINEGADMVMVKPGMPYLDVVRRVKTELQVPTFAYQVSGEYAMHKAAIMNGWL
HHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHH
KERETVFESLLCFKRAGADGILTYFAKEVAEWLAEDSAKAAQFLPKK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9529530; 10984043; 10356331; 12079382 [H]