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The map label for this gene is ubiB [H]

Identifier: 227080321

GI number: 227080321

Start: 83039

End: 84673

Strand: Direct

Name: ubiB [H]

Synonym: VCM66_0085

Alternate gene names: 227080321

Gene position: 83039-84673 (Clockwise)

Preceding gene: 227080320

Following gene: 227080322

Centisome position: 2.87

GC content: 49.17

Gene sequence:

>1635_bases
ATGAAACCTGCAGAGCTGAAACGTTTATACCGGATAGTTAAAGTTCAACTCGAATATGGCTTAGATGAGCTGTTGCCAGA
GCATCATCTGACCCGAGCGCCGCTACTGGCGCGTAAATCTCTTTTTTGGCTGCGCAATCAACACGCCGATAAAGCGTTAG
GCGATCGTTTACGTTTGGCACTGCAAGAACTTGGCCCGGTGTGGATCAAGTTTGGGCAGATGATGTCGACTCGCCGTGAC
CTATTTCCGCCGCACATCGCCGATCCCTTGGCTATGTTGCAAGATAAAGTTGCGCCGTTTGATGGCCTACAAGCCAAGCA
ATTGATTGAAGAAGAACTCGGTGCACCGCTGGAAACTTGGTTCGATGACTTTGATATCAAACCATTAGCTTCCGCGTCGA
TAGCCCAAGTACACACCGCCAAGCTCAAGAGTAATGGTCGTGATGTGGTGCTGAAAGTGATTCGTCCGGATATTCGTCCG
CAGATTGATGCCGACATCAAGCTGATGTATCGCGTGGCACGCATTGTGGCTAAAGCGCTGCCGGAAGCACGCCGCTTAAA
ACCGGTGGAAGTGGTACGCGAATACGAGAAAACCTTGCTCGATGAGTTGGACCTGCGCCGTGAAGCGGCCAATGCGATTC
AGCTGCGCCGCAACTTCGAAAACAGCGAAGAGTTGTATGTCCCTGAGGTGTTAACTGATTTCTGTAATGAAACTGTCATG
GTTTCTGAGCGAATATACGGCATCCAAGTTTCTGACTTGGCAGGACTTCATGCCAACGGTACCAATATGAAGTTACTGGC
GGAACGTGGCGTGAGTGTGTTTTTCACCCAAGTGTTTCGCGACAGTTTCTTCCATGCGGATATGCATCCGGGTAACGTGT
TCGTCAATCCTAACCATCCTGAAAACCCGCAATGGATTGGTTTGGACTGCGGCATAGTCGGGACACTGAATAGCGAAGAT
AAGCGCTATCTGGCCGAAAACTTCCTCGCCTTCTTCAATCGTGACTATCGCCGCGTAGCGCAACTGCACGTCGATTCCGG
TTGGGTACCACTGGATACCAATGTTGATGAGTTTGAAGTGGCGATCCGCATGGTGTGTGAGCCGATTTTCGCCAAACCGC
TGTGTGAGATTTCATTTGGTCACGTGTTACTGAATCTGTTTAACACCGCGCGACGCTTCAATATGGAAGTGCAGCCGCAG
TTAGTTTTGCTGCAAAAAACACTGCTGTATGTCGAAGGCTTGGGGCGTCAGCTCTACCCACAACTCGATTTATGGCAAAC
CGCCAAACCGTTTTTAGAAAAGTGGATGGCTAACCAAGTGGGGCCACAAGCTTTCTTGCATGCATTGAAAGAGCGTGCGC
CCTTATGGTTTGAAAAAATGCCTGAGCTGCCAGAATTGTTGTATGACAGCTTAAAGCAAGGTCGTAACTTGAATCAGCGT
TTAGATAATCTTTATCAAGGTTATCGCCAATCCAAACGCCAACAAGGGACGGGAAAGTTTTTGTTTGGTGTTGGAGCCAC
ATTAGTCGTATGCTCGGCGATATGGATCTCCAACCAATTAGAACCTTTAGCGATCGGTAGCGCGACCATTGGCGTGTTAT
GTTGGCTATTGAGTTGGCGAGCTTATCGCCAGTAA

Upstream 100 bases:

>100_bases
CGCCACCGTTGGAAATTGCCTATTTTTGCGATCAAGTGGATGACGTGAAAAGTCACGCTGCGCGTTTAGAAGCGCGTTTG
AACCAGTTACTGGAAAAAGC

Downstream 100 bases:

>100_bases
GCGCCTTGTCGCGTCGATAAGTTCGAAGATTTGTTCACACATAACCCGAGGTAAAAGAGATGGGTGGTATCAGTATTTGG
CAACTTCTTATCATTGCAGT

Product: putative ubiquinone biosynthesis protein UbiB

Products: 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MKPAELKRLYRIVKVQLEYGLDELLPEHHLTRAPLLARKSLFWLRNQHADKALGDRLRLALQELGPVWIKFGQMMSTRRD
LFPPHIADPLAMLQDKVAPFDGLQAKQLIEEELGAPLETWFDDFDIKPLASASIAQVHTAKLKSNGRDVVLKVIRPDIRP
QIDADIKLMYRVARIVAKALPEARRLKPVEVVREYEKTLLDELDLRREAANAIQLRRNFENSEELYVPEVLTDFCNETVM
VSERIYGIQVSDLAGLHANGTNMKLLAERGVSVFFTQVFRDSFFHADMHPGNVFVNPNHPENPQWIGLDCGIVGTLNSED
KRYLAENFLAFFNRDYRRVAQLHVDSGWVPLDTNVDEFEVAIRMVCEPIFAKPLCEISFGHVLLNLFNTARRFNMEVQPQ
LVLLQKTLLYVEGLGRQLYPQLDLWQTAKPFLEKWMANQVGPQAFLHALKERAPLWFEKMPELPELLYDSLKQGRNLNQR
LDNLYQGYRQSKRQQGTGKFLFGVGATLVVCSAIWISNQLEPLAIGSATIGVLCWLLSWRAYRQ

Sequences:

>Translated_544_residues
MKPAELKRLYRIVKVQLEYGLDELLPEHHLTRAPLLARKSLFWLRNQHADKALGDRLRLALQELGPVWIKFGQMMSTRRD
LFPPHIADPLAMLQDKVAPFDGLQAKQLIEEELGAPLETWFDDFDIKPLASASIAQVHTAKLKSNGRDVVLKVIRPDIRP
QIDADIKLMYRVARIVAKALPEARRLKPVEVVREYEKTLLDELDLRREAANAIQLRRNFENSEELYVPEVLTDFCNETVM
VSERIYGIQVSDLAGLHANGTNMKLLAERGVSVFFTQVFRDSFFHADMHPGNVFVNPNHPENPQWIGLDCGIVGTLNSED
KRYLAENFLAFFNRDYRRVAQLHVDSGWVPLDTNVDEFEVAIRMVCEPIFAKPLCEISFGHVLLNLFNTARRFNMEVQPQ
LVLLQKTLLYVEGLGRQLYPQLDLWQTAKPFLEKWMANQVGPQAFLHALKERAPLWFEKMPELPELLYDSLKQGRNLNQR
LDNLYQGYRQSKRQQGTGKFLFGVGATLVVCSAIWISNQLEPLAIGSATIGVLCWLLSWRAYRQ
>Mature_544_residues
MKPAELKRLYRIVKVQLEYGLDELLPEHHLTRAPLLARKSLFWLRNQHADKALGDRLRLALQELGPVWIKFGQMMSTRRD
LFPPHIADPLAMLQDKVAPFDGLQAKQLIEEELGAPLETWFDDFDIKPLASASIAQVHTAKLKSNGRDVVLKVIRPDIRP
QIDADIKLMYRVARIVAKALPEARRLKPVEVVREYEKTLLDELDLRREAANAIQLRRNFENSEELYVPEVLTDFCNETVM
VSERIYGIQVSDLAGLHANGTNMKLLAERGVSVFFTQVFRDSFFHADMHPGNVFVNPNHPENPQWIGLDCGIVGTLNSED
KRYLAENFLAFFNRDYRRVAQLHVDSGWVPLDTNVDEFEVAIRMVCEPIFAKPLCEISFGHVLLNLFNTARRFNMEVQPQ
LVLLQKTLLYVEGLGRQLYPQLDLWQTAKPFLEKWMANQVGPQAFLHALKERAPLWFEKMPELPELLYDSLKQGRNLNQR
LDNLYQGYRQSKRQQGTGKFLFGVGATLVVCSAIWISNQLEPLAIGSATIGVLCWLLSWRAYRQ

Specific function: Required, probably indirectly, for the hydroxylation of 2-octaprenylphenol to 2-octaprenyl-6-hydroxy-phenol, the fourth step in ubiquinone biosynthesis [H]

COG id: COG0661

COG function: function code R; Predicted unusual protein kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ABC1 family. UbiB subfamily [H]

Homologues:

Organism=Homo sapiens, GI41393593, Length=271, Percent_Identity=29.8892988929889, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI40254938, Length=278, Percent_Identity=30.9352517985612, Blast_Score=113, Evalue=6e-25,
Organism=Homo sapiens, GI32261307, Length=317, Percent_Identity=25.2365930599369, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI217035081, Length=232, Percent_Identity=30.6034482758621, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI34147522, Length=249, Percent_Identity=26.5060240963855, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI27363457, Length=195, Percent_Identity=28.7179487179487, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI2367309, Length=546, Percent_Identity=68.8644688644689, Blast_Score=778, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17559152, Length=283, Percent_Identity=27.208480565371, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32565180, Length=297, Percent_Identity=26.9360269360269, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6325148, Length=440, Percent_Identity=26.1363636363636, Blast_Score=129, Evalue=9e-31,
Organism=Saccharomyces cerevisiae, GI6323282, Length=302, Percent_Identity=25.1655629139073, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6321319, Length=200, Percent_Identity=26.5, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI22024280, Length=278, Percent_Identity=29.4964028776978, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24662575, Length=278, Percent_Identity=28.0575539568345, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI18859849, Length=198, Percent_Identity=27.7777777777778, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004147
- InterPro:   IPR011009
- InterPro:   IPR010232 [H]

Pfam domain/function: PF03109 ABC1 [H]

EC number: NA

Molecular weight: Translated: 62536; Mature: 62536

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPAELKRLYRIVKVQLEYGLDELLPEHHLTRAPLLARKSLFWLRNQHADKALGDRLRLA
CCHHHHHHHHHHHHHHHHHCHHHHCCHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
LQELGPVWIKFGQMMSTRRDLFPPHIADPLAMLQDKVAPFDGLQAKQLIEEELGAPLETW
HHHHCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHH
FDDFDIKPLASASIAQVHTAKLKSNGRDVVLKVIRPDIRPQIDADIKLMYRVARIVAKAL
HCCCCCCCCCCHHHHHHHHHHHHCCCHHEEHEECCCCCCCCCCHHHHHHHHHHHHHHHHC
PEARRLKPVEVVREYEKTLLDELDLRREAANAIQLRRNFENSEELYVPEVLTDFCNETVM
CHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHCCCCEE
VSERIYGIQVSDLAGLHANGTNMKLLAERGVSVFFTQVFRDSFFHADMHPGNVFVNPNHP
EHHHHEEEEEHHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCC
ENPQWIGLDCGIVGTLNSEDKRYLAENFLAFFNRDYRRVAQLHVDSGWVPLDTNVDEFEV
CCCCEEEECCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHH
AIRMVCEPIFAKPLCEISFGHVLLNLFNTARRFNMEVQPQLVLLQKTLLYVEGLGRQLYP
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHCC
QLDLWQTAKPFLEKWMANQVGPQAFLHALKERAPLWFEKMPELPELLYDSLKQGRNLNQR
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHCCCCHHHH
LDNLYQGYRQSKRQQGTGKFLFGVGATLVVCSAIWISNQLEPLAIGSATIGVLCWLLSWR
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHH
AYRQ
HHCC
>Mature Secondary Structure
MKPAELKRLYRIVKVQLEYGLDELLPEHHLTRAPLLARKSLFWLRNQHADKALGDRLRLA
CCHHHHHHHHHHHHHHHHHCHHHHCCHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
LQELGPVWIKFGQMMSTRRDLFPPHIADPLAMLQDKVAPFDGLQAKQLIEEELGAPLETW
HHHHCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHH
FDDFDIKPLASASIAQVHTAKLKSNGRDVVLKVIRPDIRPQIDADIKLMYRVARIVAKAL
HCCCCCCCCCCHHHHHHHHHHHHCCCHHEEHEECCCCCCCCCCHHHHHHHHHHHHHHHHC
PEARRLKPVEVVREYEKTLLDELDLRREAANAIQLRRNFENSEELYVPEVLTDFCNETVM
CHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHCCCCEE
VSERIYGIQVSDLAGLHANGTNMKLLAERGVSVFFTQVFRDSFFHADMHPGNVFVNPNHP
EHHHHEEEEEHHHCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCC
ENPQWIGLDCGIVGTLNSEDKRYLAENFLAFFNRDYRRVAQLHVDSGWVPLDTNVDEFEV
CCCCEEEECCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHH
AIRMVCEPIFAKPLCEISFGHVLLNLFNTARRFNMEVQPQLVLLQKTLLYVEGLGRQLYP
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHCC
QLDLWQTAKPFLEKWMANQVGPQAFLHALKERAPLWFEKMPELPELLYDSLKQGRNLNQR
CCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHCCCCHHHH
LDNLYQGYRQSKRQQGTGKFLFGVGATLVVCSAIWISNQLEPLAIGSATIGVLCWLLSWR
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHH
AYRQ
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 2-octaprenylphenol; O2 [C]

Specific reaction: 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA