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The map label for this gene is wecB [C]

Identifier: 226950530

GI number: 226950530

Start: 3527738

End: 3528796

Strand: Reverse

Name: wecB [C]

Synonym: CLM_3506

Alternate gene names: 226950530

Gene position: 3528796-3527738 (Counterclockwise)

Preceding gene: 226950531

Following gene: 226950529

Centisome position: 84.92

GC content: 28.9

Gene sequence:

>1059_bases
ATGAAGATATTAACAGTAGTTGGTGCACGTCCACAATTTATAAAAGCAGCAGCGGTTTCAAATATAATAAGAAAAGAGCA
TACGGAAGTATTAGTTCATACTGGACAACATTATGATGAAAATATGTCTAAAATATTTTTTCAAGAATTAAGAATACCTA
AGCCAGATTATAATTTAGAAATAGGTTCAGGTAATCATGGTGAACAGACAGGCAAAATGCTTATAGAATTAGAAAAAATA
TATTTAAAAGAAAAGCCAGATTTAGTATTAGTGTATGGAGATACAAATTCCACATTAGCAGGAGCTTTGTGTGCAAGTAA
GTTATTAATACCTGTAGCTCATGTAGAAGCTGGTCTTAGAAGTTTTAATATGAATATGCCAGAAGAACAAAATAGAATTC
TTACGGATCATATATCAAAGTTATTATTTGTACCTACAGTTACAGCAGAAAAAAATCTTCATACTGAGGGAGTTGATAAC
GGTGTTCATAATATAGGAGATGTAATGTTTGATGCTGTATTACATTTTAAGAAATTAGCGGAAGAAAAGGAAAGTATATT
AGACAAGATTTCTATAAAATCTGGAGAATATATTTTAACAACAATTCACAGAGCAGAAAACACTAATGATATAAATAGAT
TGAAAAATATAATAGAGGCATTAAATGAAAGCGGAAAGTCAATAGTATTACCACTTCATCCAAGAACTAAAAAATATATA
GAAGATTATAATTTACAATTAAATGATAATATAAAATTAATAGAGCCAGTAGGATATCTTGATATGATTACTTTAGAAAT
GAATTCGCAAAAGATAGTTACTGATAGCGGTGGAGTTCAAAAAGAAGCTTTCTTTATGGAAAAACCTTGTGTTACTATGA
GAGATGAAACAGAATGGGTAGAGACCGTAGAAAATGGATGGAATATAATAGTAGGTACAAATAAAGAAAAAATATTAAAT
GCTATAGTAAAATTTAAGCCTGAAAAAGAACAACAAGAAATATTTGGGAATGGCCATGCAGCGGAAAAAATATTAGATAT
AATAAATGAAATGAAGTAA

Upstream 100 bases:

>100_bases
AAACCGCAAGGAAGTTTGTGGAAGATAATTTTAATATAGAAGATAATTTTAATAATGTAGATATAATATATAAATCTATA
ATAGATGGAGGAGATAATTA

Downstream 100 bases:

>100_bases
TTATTTTGAGGTGATAATATGAAAATTCTATTTTTGACTCAATACTGTCCACCTGAAGTTGGAGCACCTCAAAACAGAAT
TTTTGAGTTTGCAAAGCAAC

Product: UDP-N-acetylglucosamine 2-epimerase

Products: UDP-N-acetyl-D-mannosamine

Alternate protein names: NA

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MKILTVVGARPQFIKAAAVSNIIRKEHTEVLVHTGQHYDENMSKIFFQELRIPKPDYNLEIGSGNHGEQTGKMLIELEKI
YLKEKPDLVLVYGDTNSTLAGALCASKLLIPVAHVEAGLRSFNMNMPEEQNRILTDHISKLLFVPTVTAEKNLHTEGVDN
GVHNIGDVMFDAVLHFKKLAEEKESILDKISIKSGEYILTTIHRAENTNDINRLKNIIEALNESGKSIVLPLHPRTKKYI
EDYNLQLNDNIKLIEPVGYLDMITLEMNSQKIVTDSGGVQKEAFFMEKPCVTMRDETEWVETVENGWNIIVGTNKEKILN
AIVKFKPEKEQQEIFGNGHAAEKILDIINEMK

Sequences:

>Translated_352_residues
MKILTVVGARPQFIKAAAVSNIIRKEHTEVLVHTGQHYDENMSKIFFQELRIPKPDYNLEIGSGNHGEQTGKMLIELEKI
YLKEKPDLVLVYGDTNSTLAGALCASKLLIPVAHVEAGLRSFNMNMPEEQNRILTDHISKLLFVPTVTAEKNLHTEGVDN
GVHNIGDVMFDAVLHFKKLAEEKESILDKISIKSGEYILTTIHRAENTNDINRLKNIIEALNESGKSIVLPLHPRTKKYI
EDYNLQLNDNIKLIEPVGYLDMITLEMNSQKIVTDSGGVQKEAFFMEKPCVTMRDETEWVETVENGWNIIVGTNKEKILN
AIVKFKPEKEQQEIFGNGHAAEKILDIINEMK
>Mature_352_residues
MKILTVVGARPQFIKAAAVSNIIRKEHTEVLVHTGQHYDENMSKIFFQELRIPKPDYNLEIGSGNHGEQTGKMLIELEKI
YLKEKPDLVLVYGDTNSTLAGALCASKLLIPVAHVEAGLRSFNMNMPEEQNRILTDHISKLLFVPTVTAEKNLHTEGVDN
GVHNIGDVMFDAVLHFKKLAEEKESILDKISIKSGEYILTTIHRAENTNDINRLKNIIEALNESGKSIVLPLHPRTKKYI
EDYNLQLNDNIKLIEPVGYLDMITLEMNSQKIVTDSGGVQKEAFFMEKPCVTMRDETEWVETVENGWNIIVGTNKEKILN
AIVKFKPEKEQQEIFGNGHAAEKILDIINEMK

Specific function: Catalyzes The Reversible Epimerization At C-2 Of UDP-N- Acetylglucosamine (UDP-Glcnac) And Thereby Provides Bacteria With UDP-N-Acetylmannosamine (UDP-Mannac), The Activated Donor Of Mannac Residues. Also Involved In Bacteriophage N4 Adsorption. [C]

COG id: COG0381

COG function: function code M; UDP-N-acetylglucosamine 2-epimerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-N-acetylglucosamine 2-epimerase family [H]

Homologues:

Organism=Escherichia coli, GI48994967, Length=378, Percent_Identity=34.3915343915344, Blast_Score=149, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003331 [H]

Pfam domain/function: PF02350 Epimerase_2 [H]

EC number: 5.1.3.14

Molecular weight: Translated: 39813; Mature: 39813

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILTVVGARPQFIKAAAVSNIIRKEHTEVLVHTGQHYDENMSKIFFQELRIPKPDYNLE
CEEEEEECCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHCCCCCCCEEE
IGSGNHGEQTGKMLIELEKIYLKEKPDLVLVYGDTNSTLAGALCASKLLIPVAHVEAGLR
ECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
SFNMNMPEEQNRILTDHISKLLFVPTVTAEKNLHTEGVDNGVHNIGDVMFDAVLHFKKLA
HCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
EEKESILDKISIKSGEYILTTIHRAENTNDINRLKNIIEALNESGKSIVLPLHPRTKKYI
HHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHH
EDYNLQLNDNIKLIEPVGYLDMITLEMNSQKIVTDSGGVQKEAFFMEKPCVTMRDETEWV
HHCCEEECCCEEEEECCCCEEEEEEEECCCEEEECCCCCCHHHHHHCCCCCCCCCCHHHH
ETVENGWNIIVGTNKEKILNAIVKFKPEKEQQEIFGNGHAAEKILDIINEMK
HHHHCCCEEEEECCHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKILTVVGARPQFIKAAAVSNIIRKEHTEVLVHTGQHYDENMSKIFFQELRIPKPDYNLE
CEEEEEECCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHCCCCCCCEEE
IGSGNHGEQTGKMLIELEKIYLKEKPDLVLVYGDTNSTLAGALCASKLLIPVAHVEAGLR
ECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
SFNMNMPEEQNRILTDHISKLLFVPTVTAEKNLHTEGVDNGVHNIGDVMFDAVLHFKKLA
HCCCCCCHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
EEKESILDKISIKSGEYILTTIHRAENTNDINRLKNIIEALNESGKSIVLPLHPRTKKYI
HHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHH
EDYNLQLNDNIKLIEPVGYLDMITLEMNSQKIVTDSGGVQKEAFFMEKPCVTMRDETEWV
HHCCEEECCCEEEEECCCCEEEEEEEECCCEEEECCCCCCHHHHHHCCCCCCCCCCHHHH
ETVENGWNIIVGTNKEKILNAIVKFKPEKEQQEIFGNGHAAEKILDIINEMK
HHHHCCCEEEEECCHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: UDP-N-acetylglucosamine [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.63 {UDP-N-acetylglucosamine}} 1 {UDP-N-acetylmannosamine}} 0.5 {UDP-N-acetylmannosamine}} [C]

Substrates: UDP-N-acetyl-D-glucosamine

Specific reaction: UDP-N-acetyl-D-glucosamine = UDP-N-acetyl-D-mannosamine

General reaction: Epimerization [C]

Inhibitor: PCMB [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA