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The map label for this gene is dacB [H]

Identifier: 226950403

GI number: 226950403

Start: 3410425

End: 3411672

Strand: Direct

Name: dacB [H]

Synonym: CLM_3374

Alternate gene names: 226950403

Gene position: 3410425-3411672 (Clockwise)

Preceding gene: 226950393

Following gene: 226950432

Centisome position: 82.07

GC content: 27.16

Gene sequence:

>1248_bases
ATGAAGCTCATTTTTTATAAAATATTATGTTCTATTTTATTATTCTCCTTATTTTTTTCTAATGTAGCTTTGGCAAAGGA
TTCTAAACCAAACTTAAAAATAGCCGCTGATGGTGTTGTATTAATGGATTCAAAAACCGGTAAAATAATTTATTCAAAAA
ATCCTGATAAACCATATCCTCCAGCTTCCACTACAAAAATAATGACTGCTCTTTTAACTTTAGAAAGAGGTAATTTAGAT
GACATCGTCACTATAGGTCGTAAGCCCCCTTTAGCTGAAGGAAGCAAGATATACATATTAGAAGGAGAGAAAATTAAAGT
AAGAGATCTATTATATGGGCTTTTACTAGCCTCAGCTAACGATTGTGCTGAAGCATTAGCTGAATATCTAAGTGGGTCTT
TGGATAATTTTTCAAAAGATATGAATAAACGAGCTAAAGAATTAGGTTGTATCAATACTAATTTCGTAAATCCTAGTGGA
TTGTATAATGATAAGCATAGAACTTCTGCTAGAGATTTAGCTATTATTATGAATGAACTAGTTAAACAACCAGAATACAG
TAAAATAGCCACAACTCTTTCTTACAATATTCCACCTACAAATAAATCAAAAGAAAAAAGACCTTTGTGGAATGAAAACA
AACTTATACAAAAACATTCAAATTTTTATTACAAAGGTTGCGAAGGTGGAAAAACAGGATATACTGTTCAATCAGATCAT
TCTTATGTAGCCAGTGTTAATAAGAATAATCATAGAATTATAGTAGCTCTTGTTCATGATAAAGAAAAAAATTTTTTTGA
TGATTCAGTAAAACTATTTAACTATGGATTTAATAATTTTACATTAGATCTTCTATATCCTAAAGATTCTTACGTAACTA
CTTATACAAATGACAACTTAAAAGTTCCATTATATGCCTCAAAAGATTTTTATTATCTAAAACCAAAGGATGATAATATG
GCTCCTGATTTAAAATTAGAAAATACAAATTTAAAAAATCTAAAATTTAAAAAAGGTGATGTAGTTTTAAGTGCCAATTT
AAGTCGAGGCTCTAATAGTGTAGGAAGCCTAACTTTAAAAAGTGGTGTAGATCATAAATCAGTTAACTTATTTACTAATA
ATGTTATTAGCAAACATTTAAACACCAGAAATATAATTTTAATATGCTTAGCATCAATATTTTTAACATTTAAAATTACA
CGAAAAATTTTAAGATCAAAAAAAAACAACAATAAATATTATTTTTAA

Upstream 100 bases:

>100_bases
CTAATTATTGACATATTTATAATTTTAATTCGCATATTAATGTTTTTTTTGATATAATAAAAATTATTATTATGTAGAAA
CAGGAGGTTTACTTAATATA

Downstream 100 bases:

>100_bases
TACAAAAGCTATGAAATATAGATTTAATAACATCTTAATTTCATAGCTTTTATTTTTTTTATGGTAACTATACAAAATCT
TTGTAAAAGTTATTTCACAT

Product: D-alanyl-D-alanine carboxypeptidase family protein

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MKLIFYKILCSILLFSLFFSNVALAKDSKPNLKIAADGVVLMDSKTGKIIYSKNPDKPYPPASTTKIMTALLTLERGNLD
DIVTIGRKPPLAEGSKIYILEGEKIKVRDLLYGLLLASANDCAEALAEYLSGSLDNFSKDMNKRAKELGCINTNFVNPSG
LYNDKHRTSARDLAIIMNELVKQPEYSKIATTLSYNIPPTNKSKEKRPLWNENKLIQKHSNFYYKGCEGGKTGYTVQSDH
SYVASVNKNNHRIIVALVHDKEKNFFDDSVKLFNYGFNNFTLDLLYPKDSYVTTYTNDNLKVPLYASKDFYYLKPKDDNM
APDLKLENTNLKNLKFKKGDVVLSANLSRGSNSVGSLTLKSGVDHKSVNLFTNNVISKHLNTRNIILICLASIFLTFKIT
RKILRSKKNNNKYYF

Sequences:

>Translated_415_residues
MKLIFYKILCSILLFSLFFSNVALAKDSKPNLKIAADGVVLMDSKTGKIIYSKNPDKPYPPASTTKIMTALLTLERGNLD
DIVTIGRKPPLAEGSKIYILEGEKIKVRDLLYGLLLASANDCAEALAEYLSGSLDNFSKDMNKRAKELGCINTNFVNPSG
LYNDKHRTSARDLAIIMNELVKQPEYSKIATTLSYNIPPTNKSKEKRPLWNENKLIQKHSNFYYKGCEGGKTGYTVQSDH
SYVASVNKNNHRIIVALVHDKEKNFFDDSVKLFNYGFNNFTLDLLYPKDSYVTTYTNDNLKVPLYASKDFYYLKPKDDNM
APDLKLENTNLKNLKFKKGDVVLSANLSRGSNSVGSLTLKSGVDHKSVNLFTNNVISKHLNTRNIILICLASIFLTFKIT
RKILRSKKNNNKYYF
>Mature_415_residues
MKLIFYKILCSILLFSLFFSNVALAKDSKPNLKIAADGVVLMDSKTGKIIYSKNPDKPYPPASTTKIMTALLTLERGNLD
DIVTIGRKPPLAEGSKIYILEGEKIKVRDLLYGLLLASANDCAEALAEYLSGSLDNFSKDMNKRAKELGCINTNFVNPSG
LYNDKHRTSARDLAIIMNELVKQPEYSKIATTLSYNIPPTNKSKEKRPLWNENKLIQKHSNFYYKGCEGGKTGYTVQSDH
SYVASVNKNNHRIIVALVHDKEKNFFDDSVKLFNYGFNNFTLDLLYPKDSYVTTYTNDNLKVPLYASKDFYYLKPKDDNM
APDLKLENTNLKNLKFKKGDVVLSANLSRGSNSVGSLTLKSGVDHKSVNLFTNNVISKHLNTRNIILICLASIFLTFKIT
RKILRSKKNNNKYYF

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=350, Percent_Identity=30, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI87082022, Length=300, Percent_Identity=29, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1786851, Length=300, Percent_Identity=29.3333333333333, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI87082054, Length=252, Percent_Identity=30.1587301587302, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 46891; Mature: 46891

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIFYKILCSILLFSLFFSNVALAKDSKPNLKIAADGVVLMDSKTGKIIYSKNPDKPYP
CEEEHHHHHHHHHHHHHHHCCEEEECCCCCCEEEEECCEEEEECCCCEEEEECCCCCCCC
PASTTKIMTALLTLERGNLDDIVTIGRKPPLAEGSKIYILEGEKIKVRDLLYGLLLASAN
CCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCEEEEEECCEEEHHHHHHHHHHHCCH
DCAEALAEYLSGSLDNFSKDMNKRAKELGCINTNFVNPSGLYNDKHRTSARDLAIIMNEL
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
VKQPEYSKIATTLSYNIPPTNKSKEKRPLWNENKLIQKHSNFYYKGCEGGKTGYTVQSDH
HCCCCCHHEEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCCEEEECCCCCCCCCEEECCC
SYVASVNKNNHRIIVALVHDKEKNFFDDSVKLFNYGFNNFTLDLLYPKDSYVTTYTNDNL
CEEEEECCCCCEEEEEEEECCCCCCCCCCEEEEEECCCCEEEEEEECCCCEEEEEECCCE
KVPLYASKDFYYLKPKDDNMAPDLKLENTNLKNLKFKKGDVVLSANLSRGSNSVGSLTLK
EEEEEECCCEEEECCCCCCCCCCEEECCCCCCCEEEECCCEEEEECCCCCCCCCCEEEEE
SGVDHKSVNLFTNNVISKHLNTRNIILICLASIFLTFKITRKILRSKKNNNKYYF
CCCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKLIFYKILCSILLFSLFFSNVALAKDSKPNLKIAADGVVLMDSKTGKIIYSKNPDKPYP
CEEEHHHHHHHHHHHHHHHCCEEEECCCCCCEEEEECCEEEEECCCCEEEEECCCCCCCC
PASTTKIMTALLTLERGNLDDIVTIGRKPPLAEGSKIYILEGEKIKVRDLLYGLLLASAN
CCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCEEEEEECCEEEHHHHHHHHHHHCCH
DCAEALAEYLSGSLDNFSKDMNKRAKELGCINTNFVNPSGLYNDKHRTSARDLAIIMNEL
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
VKQPEYSKIATTLSYNIPPTNKSKEKRPLWNENKLIQKHSNFYYKGCEGGKTGYTVQSDH
HCCCCCHHEEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCCEEEECCCCCCCCCEEECCC
SYVASVNKNNHRIIVALVHDKEKNFFDDSVKLFNYGFNNFTLDLLYPKDSYVTTYTNDNL
CEEEEECCCCCEEEEEEEECCCCCCCCCCEEEEEECCCCEEEEEEECCCCEEEEEECCCE
KVPLYASKDFYYLKPKDDNMAPDLKLENTNLKNLKFKKGDVVLSANLSRGSNSVGSLTLK
EEEEEECCCEEEECCCCCCCCCCEEECCCCCCCEEEECCCEEEEECCCCCCCCCCEEEEE
SGVDHKSVNLFTNNVISKHLNTRNIILICLASIFLTFKITRKILRSKKNNNKYYF
CCCCCCEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]