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The map label for this gene is grpE [H]

Identifier: 226950386

GI number: 226950386

Start: 3387495

End: 3388139

Strand: Reverse

Name: grpE [H]

Synonym: CLM_3355

Alternate gene names: 226950386

Gene position: 3388139-3387495 (Counterclockwise)

Preceding gene: 226950387

Following gene: 226950385

Centisome position: 81.54

GC content: 29.3

Gene sequence:

>645_bases
TTGGAAAAAGAATGCAAGGATGCTAAACATATTAACATGGAAGAGGATTGCTGCTGCTCTAATAAAGAAAGTGAAAATAA
AGAAGAAGATAAAGGTAAAGAAGAGGATTTAGAGTTTGAAGAAATAGAAAAAGAAGAAATAATAGAGGACTCTGAAAATT
CTAATGAAGTAAAGATAAAGGAATTAGAAGATATGAAAAATAAATTAAAAGAAGAAAATAAAAAATTAGAAAATCAGATG
GAAGAAATAAAGGAAAGATTAGTAAGAACAGTAGCAGAATATGATAATTTTAGAAAAAGAACAGCTAAAGAAAAAGAAGA
CTTATATGTTAGTGCCTGTGAAGATGTCTTAAAAGAACTTCTTCCGGTTTTAGATAATTTGGAAAGAGCAGCAAACGTAG
AGGGTTCTGTAGAAGATATTAAAAAAGGTATAGACATGACAGTTAAACAGTTTGGAACTTCTTTAGAAAAATTAGGGGTA
GAAGAGATATCTACAGAAGTTGCTTTTGATCCTAATATTCATAATGCAGTAATGCATGTGGAGGATTCCAATTGTGGAGA
AAAGGAAATAGTAGAAGTATTCCAAAAGGGATATAAAAAAGGTGAAAAAATTATAAGATATAGTATGGTAAAAGTAGCAA
ACTAG

Upstream 100 bases:

>100_bases
TAATATCAGCTGTGGCTACAATAGTTAAGGAAATAAATGATACCTTAACTAATGACAAAAATATTCATAAATAACAAGCG
GATAGAATGGAGGTATATAA

Downstream 100 bases:

>100_bases
ATAAAAATTTATTATAAAAACCTAACAAATTACAAACTAGGAGGGAATATTAAAATGGCAAAAATAATCGGAATTGACTT
AGGAACAACAAATTCTTGTG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MEKECKDAKHINMEEDCCCSNKESENKEEDKGKEEDLEFEEIEKEEIIEDSENSNEVKIKELEDMKNKLKEENKKLENQM
EEIKERLVRTVAEYDNFRKRTAKEKEDLYVSACEDVLKELLPVLDNLERAANVEGSVEDIKKGIDMTVKQFGTSLEKLGV
EEISTEVAFDPNIHNAVMHVEDSNCGEKEIVEVFQKGYKKGEKIIRYSMVKVAN

Sequences:

>Translated_214_residues
MEKECKDAKHINMEEDCCCSNKESENKEEDKGKEEDLEFEEIEKEEIIEDSENSNEVKIKELEDMKNKLKEENKKLENQM
EEIKERLVRTVAEYDNFRKRTAKEKEDLYVSACEDVLKELLPVLDNLERAANVEGSVEDIKKGIDMTVKQFGTSLEKLGV
EEISTEVAFDPNIHNAVMHVEDSNCGEKEIVEVFQKGYKKGEKIIRYSMVKVAN
>Mature_214_residues
MEKECKDAKHINMEEDCCCSNKESENKEEDKGKEEDLEFEEIEKEEIIEDSENSNEVKIKELEDMKNKLKEENKKLENQM
EEIKERLVRTVAEYDNFRKRTAKEKEDLYVSACEDVLKELLPVLDNLERAANVEGSVEDIKKGIDMTVKQFGTSLEKLGV
EEISTEVAFDPNIHNAVMHVEDSNCGEKEIVEVFQKGYKKGEKIIRYSMVKVAN

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Escherichia coli, GI1788967, Length=147, Percent_Identity=36.0544217687075, Blast_Score=89, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324806, Length=159, Percent_Identity=28.3018867924528, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 24820; Mature: 24820

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKECKDAKHINMEEDCCCSNKESENKEEDKGKEEDLEFEEIEKEEIIEDSENSNEVKIK
CCCCCCHHHCCCCCHHHCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHH
ELEDMKNKLKEENKKLENQMEEIKERLVRTVAEYDNFRKRTAKEKEDLYVSACEDVLKEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPVLDNLERAANVEGSVEDIKKGIDMTVKQFGTSLEKLGVEEISTEVAFDPNIHNAVMHV
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEE
EDSNCGEKEIVEVFQKGYKKGEKIIRYSMVKVAN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEKECKDAKHINMEEDCCCSNKESENKEEDKGKEEDLEFEEIEKEEIIEDSENSNEVKIK
CCCCCCHHHCCCCCHHHCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCHHH
ELEDMKNKLKEENKKLENQMEEIKERLVRTVAEYDNFRKRTAKEKEDLYVSACEDVLKEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPVLDNLERAANVEGSVEDIKKGIDMTVKQFGTSLEKLGVEEISTEVAFDPNIHNAVMHV
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEE
EDSNCGEKEIVEVFQKGYKKGEKIIRYSMVKVAN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]