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The map label for this gene is vfr [H]

Identifier: 226950351

GI number: 226950351

Start: 3348562

End: 3349239

Strand: Direct

Name: vfr [H]

Synonym: CLM_3318

Alternate gene names: 226950351

Gene position: 3348562-3349239 (Clockwise)

Preceding gene: 226950349

Following gene: 226950352

Centisome position: 80.59

GC content: 23.01

Gene sequence:

>678_bases
ATGAAAAATATTTTTTTTATATTAAGTAAATGTATATTATTTAAAAATTTTGAGGAAAAATGCATAGAAAAAATTCTTAA
GAATATAAATTATAAAGTAAAAGATTATAAAAAAGATGAAGTAATAGCTATAGAAGGTGATGCCTGCTCTACTATAGGTA
TTATATTAGATGGGAGCATAGAAATACAAAAATTATTTTCTTCTGGAAAAACCGTTACTATTACAACCTTATCGACTGGA
GATATTTTTGGTGAAGTTATTGTTTTTTCTTCTATGAATAAATATCCTTCTACTATACTATCTAACTCTAATAGTAGTGT
TATGTTTATATCTAAGGAAGATATTATTAAGCTTTGTAGTTTAGACTCTTTAATTTTAAATAACTTAATGGGACTTCTAT
CTAATAAAATATTAATGTTGAATAAAAAAGTTAAAAATTTATCTTATGAAACTATTCGTCAAAAAATTTCTAGTTTTTTA
GTGGATCAATATAGAAAACAAAATAGCTTAACTTTAAAATTAAATATGTCCAGACAGACTTTAGCTGAAATCTTTGGTAT
ACCTAGACCTTCTCTATCCAGGGAACTTATCAATATGAAAAATGATAAGTTAATAGATGTTGAAAAAAATATAATAATCA
TAAAAGACTTAGAAGCCTTAGAAGAAATTTTATATTAA

Upstream 100 bases:

>100_bases
TATTCCTCCCTGTTGATTTTATACATTTATTATAGTACTATTATGTAAATAATTCTGTAACTAATGTTACCATCATAAAA
TTTATGGGGGGTTATTTTTT

Downstream 100 bases:

>100_bases
TTTTAATAAAAGTTTAAAATAATTAGTACTTTTTTTGGTACTATGAATAAATTAATATTGTAATGACTTTTTATGAGGTG
TATTTTATATGGCATATTCC

Product: cyclic nucleotide-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MKNIFFILSKCILFKNFEEKCIEKILKNINYKVKDYKKDEVIAIEGDACSTIGIILDGSIEIQKLFSSGKTVTITTLSTG
DIFGEVIVFSSMNKYPSTILSNSNSSVMFISKEDIIKLCSLDSLILNNLMGLLSNKILMLNKKVKNLSYETIRQKISSFL
VDQYRKQNSLTLKLNMSRQTLAEIFGIPRPSLSRELINMKNDKLIDVEKNIIIIKDLEALEEILY

Sequences:

>Translated_225_residues
MKNIFFILSKCILFKNFEEKCIEKILKNINYKVKDYKKDEVIAIEGDACSTIGIILDGSIEIQKLFSSGKTVTITTLSTG
DIFGEVIVFSSMNKYPSTILSNSNSSVMFISKEDIIKLCSLDSLILNNLMGLLSNKILMLNKKVKNLSYETIRQKISSFL
VDQYRKQNSLTLKLNMSRQTLAEIFGIPRPSLSRELINMKNDKLIDVEKNIIIIKDLEALEEILY
>Mature_225_residues
MKNIFFILSKCILFKNFEEKCIEKILKNINYKVKDYKKDEVIAIEGDACSTIGIILDGSIEIQKLFSSGKTVTITTLSTG
DIFGEVIVFSSMNKYPSTILSNSNSSVMFISKEDIIKLCSLDSLILNNLMGLLSNKILMLNKKVKNLSYETIRQKISSFL
VDQYRKQNSLTLKLNMSRQTLAEIFGIPRPSLSRELINMKNDKLIDVEKNIIIIKDLEALEEILY

Specific function: Can bind cyclic AMP. Is a global regulator of virulence factor expression and is required for exotoxin A and protease production [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=207, Percent_Identity=20.2898550724638, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR018488
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 25625; Mature: 25625

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIFFILSKCILFKNFEEKCIEKILKNINYKVKDYKKDEVIAIEGDACSTIGIILDGSI
CCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEECCCCCCEEEEECCCCCEEEEEEECCH
EIQKLFSSGKTVTITTLSTGDIFGEVIVFSSMNKYPSTILSNSNSSVMFISKEDIIKLCS
HHHHHHHCCCEEEEEEEECCHHHHHHHHHHCCCCCCHHHHCCCCCCEEEEEHHHHHHHHH
LDSLILNNLMGLLSNKILMLNKKVKNLSYETIRQKISSFLVDQYRKQNSLTLKLNMSRQT
HHHHHHHHHHHHHCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHH
LAEIFGIPRPSLSRELINMKNDKLIDVEKNIIIIKDLEALEEILY
HHHHHCCCCCCHHHHHHCCCCCEEEEECCCEEEEECHHHHHHHHC
>Mature Secondary Structure
MKNIFFILSKCILFKNFEEKCIEKILKNINYKVKDYKKDEVIAIEGDACSTIGIILDGSI
CCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEECCCCCCEEEEECCCCCEEEEEEECCH
EIQKLFSSGKTVTITTLSTGDIFGEVIVFSSMNKYPSTILSNSNSSVMFISKEDIIKLCS
HHHHHHHCCCEEEEEEEECCHHHHHHHHHHCCCCCCHHHHCCCCCCEEEEEHHHHHHHHH
LDSLILNNLMGLLSNKILMLNKKVKNLSYETIRQKISSFLVDQYRKQNSLTLKLNMSRQT
HHHHHHHHHHHHHCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHH
LAEIFGIPRPSLSRELINMKNDKLIDVEKNIIIIKDLEALEEILY
HHHHHCCCCCCHHHHHHCCCCCEEEEECCCEEEEECHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8002577; 10984043 [H]