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The map label for this gene is cwlJ [H]

Identifier: 226950352

GI number: 226950352

Start: 3349328

End: 3349771

Strand: Direct

Name: cwlJ [H]

Synonym: CLM_3319

Alternate gene names: 226950352

Gene position: 3349328-3349771 (Clockwise)

Preceding gene: 226950351

Following gene: 226950353

Centisome position: 80.6

GC content: 36.26

Gene sequence:

>444_bases
ATGGCATATTCCGATAGAGAATTATTAGCTAGAATTATAAAATGTGAAGCTGGTGGGGAAGGCGATAATGGAATGAGAGC
TGTAGCTACTGTAGTTATGAATAGAGTTAGAGTGCCTTATGGTGAATATCACAGAATTGGTCAAGGTAATTTAAGAAATG
TTATTTATCAACCTGGACAATTTGATTGTGTAAGAGATGTATTAAGAGGAATTCCTAACCCTCAAACTATTTGGGCTACT
CCACCAGAGGAGATACACTATGAGATAGCCGATTGGGCATTATCTGGTAATAGACTATATAATATAGGATTTTCCCTCTG
GTACTTTAATCCTTTTCAGCCAAGTTGTCCGTATACCTTCCCAGCAAATGGTACTGGTTCTTTTCAAGTAAGAGTTATTC
AACATTGCTTTTATAATCCAACTGAACTATATGCGCAAACTTAA

Upstream 100 bases:

>100_bases
ATTTTATATTAATTTTAATAAAAGTTTAAAATAATTAGTACTTTTTTTGGTACTATGAATAAATTAATATTGTAATGACT
TTTTATGAGGTGTATTTTAT

Downstream 100 bases:

>100_bases
TAAATAAATTAGGAGGAATTTTATGTTTATTGATGATTATCCTTATCTAGAAAATAATCAGAATGATGAAAATATGAATA
TGAGGCCTATGCCTTACATG

Product: putative cell wall hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 147; Mature: 146

Protein sequence:

>147_residues
MAYSDRELLARIIKCEAGGEGDNGMRAVATVVMNRVRVPYGEYHRIGQGNLRNVIYQPGQFDCVRDVLRGIPNPQTIWAT
PPEEIHYEIADWALSGNRLYNIGFSLWYFNPFQPSCPYTFPANGTGSFQVRVIQHCFYNPTELYAQT

Sequences:

>Translated_147_residues
MAYSDRELLARIIKCEAGGEGDNGMRAVATVVMNRVRVPYGEYHRIGQGNLRNVIYQPGQFDCVRDVLRGIPNPQTIWAT
PPEEIHYEIADWALSGNRLYNIGFSLWYFNPFQPSCPYTFPANGTGSFQVRVIQHCFYNPTELYAQT
>Mature_146_residues
AYSDRELLARIIKCEAGGEGDNGMRAVATVVMNRVRVPYGEYHRIGQGNLRNVIYQPGQFDCVRDVLRGIPNPQTIWATP
PEEIHYEIADWALSGNRLYNIGFSLWYFNPFQPSCPYTFPANGTGSFQVRVIQHCFYNPTELYAQT

Specific function: Probable spore cortex-lytic enzyme involved in the depolymerization of cortex peptidoglycan during germination [H]

COG id: COG3773

COG function: function code M; Cell wall hydrolyses involved in spore germination

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CwlJ family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011105 [H]

Pfam domain/function: PF07486 Hydrolase_2 [H]

EC number: 3.5.1.28

Molecular weight: Translated: 16769; Mature: 16638

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYSDRELLARIIKCEAGGEGDNGMRAVATVVMNRVRVPYGEYHRIGQGNLRNVIYQPGQ
CCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCHHEEECCCC
FDCVRDVLRGIPNPQTIWATPPEEIHYEIADWALSGNRLYNIGFSLWYFNPFQPSCPYTF
CHHHHHHHHCCCCCCEEECCCHHHHEEHHHHEEECCCEEEEEEEEEEEECCCCCCCCEEC
PANGTGSFQVRVIQHCFYNPTELYAQT
CCCCCCCEEEEEHHHHHCCCHHHEECC
>Mature Secondary Structure 
AYSDRELLARIIKCEAGGEGDNGMRAVATVVMNRVRVPYGEYHRIGQGNLRNVIYQPGQ
CCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCHHEEECCCC
FDCVRDVLRGIPNPQTIWATPPEEIHYEIADWALSGNRLYNIGFSLWYFNPFQPSCPYTF
CHHHHHHHHCCCCCCEEECCCHHHHEEHHHHEEECCCEEEEEEEEEEEECCCCCCCCEEC
PANGTGSFQVRVIQHCFYNPTELYAQT
CCCCCCCEEEEEHHHHHCCCHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704254; 9384377; 9515903; 11466292; 11807087; 12177332 [H]