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The map label for this gene is ribAB [H]

Identifier: 226950298

GI number: 226950298

Start: 3281534

End: 3282739

Strand: Reverse

Name: ribAB [H]

Synonym: CLM_3260

Alternate gene names: 226950298

Gene position: 3282739-3281534 (Counterclockwise)

Preceding gene: 226950299

Following gene: 226950297

Centisome position: 79.0

GC content: 31.51

Gene sequence:

>1206_bases
ATGAGTTATAAATTTAATACTATAGAAGAGGCAATAGAGGATATAAAAGATGGAAAGATTATTGTAGTTGTAGATGATGA
GGATAGAGAGAACGAGGGAGATCTTTTAATGGCAGCAGAAAAAATTACTCCAGAGGCTATAAATTTTATGGCTAAAGAGG
GAAGAGGGCTTATATGTACTCCCGTAAAAAAATCTAGATTGGAAGAATTAGAAATATGCCAAATGGTACATAAAAATAAA
GATAATTTTGGTACCGCTTTTACTGTATCAGTAGATGCAGTAGGTACATCTACTGGAATATCTGCATTTGATAGAGCATT
TACAGTATCAAAAATAGTAGATCTAAATTCAAAACCAGAGGATTTTTTAAAGCCAGGCCATGTATTTCCTTTAGCTGCTA
AAGATGGAGGAGTTTTAGTTAGAGCAGGTCATACAGAAGCAGCAGTAGATATGGCAAGATTATCAGGTTTTTCTGAGGTA
GGAGTTATATGTGAAATAATGAATGAGGATGGAACTATGGCAAGAGTGCCACAGCTTATGGAATTTGTAAAAAAACATAA
TTTAAAAATAATAACTATAGCAGATTTAATTGAATATAGAAGTAAAGAAGAAAAATTAATAAAGGCTTTAGAACCAGTAA
AACTTCCATCTAAATATGGAGACTTTATTGCTATAGGTTATGAAAATTTAATAACTGGAGAAGAACATATAGCTTTAATA
AAAGGAGATGTAAAAGGAGAAGATCCAGTACTTGTTAGAGTACATTCAGAATGTTTAACAGGAGATGTATTCGGTTCTTT
AAGATGCGATTGTGGTGAGCAAATAGCTAAAGCTCTTATGAAAATAGAAGAAGAAGGGAAAGGAGTTTTAGTTTATATGA
GACAGGAAGGGAGAGGAATAGGTTTATTAAATAAACTAAAGGCATATGCTCTTCAAGATGAAGGTATGGATACTATAGAA
GCAAATTTAGCTTTAGGATTCCCAGAGGACTTAAGAGATTATGGCATAGGAGCTCAAATATTAAAGGATTTAGGAATTAA
AAATATTAAACTTATGACTAATAATCCTAAAAAAATATCAGGACTTTCAGGTTATGGTATAAAAATAGTAGATAGAGTTC
CTATAGATATGGGCTGTAAAAAAGAAAATCGTAATTATTTAAGAACTAAGAAAGATAAAATGGGACACTTGATAGACATA
ATGTAG

Upstream 100 bases:

>100_bases
TGCTATATATACATCTCATAGATTACAGGTAGGGCACTATATTCAATAAATTGTGTAAAATTTTTCAGTAAATCTAAAAA
TATATTTAGGGGGAATTTTC

Downstream 100 bases:

>100_bases
ATAAATTAATAAAAGATTAGGGGGAAATAAAATGAAAATATATGAAGGTAGATTAACAGCAGAAGGTTTAAAGGTAGGAA
TAATAGTTTCAAGATTTAAT

Product: bifunctional riboflavin biosynthesis protein RibAB

Products: NA

Alternate protein names: 3,4-dihydroxy-2-butanone 4-phosphate synthase; DHBP synthase; GTP cyclohydrolase-2; GTP cyclohydrolase II [H]

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MSYKFNTIEEAIEDIKDGKIIVVVDDEDRENEGDLLMAAEKITPEAINFMAKEGRGLICTPVKKSRLEELEICQMVHKNK
DNFGTAFTVSVDAVGTSTGISAFDRAFTVSKIVDLNSKPEDFLKPGHVFPLAAKDGGVLVRAGHTEAAVDMARLSGFSEV
GVICEIMNEDGTMARVPQLMEFVKKHNLKIITIADLIEYRSKEEKLIKALEPVKLPSKYGDFIAIGYENLITGEEHIALI
KGDVKGEDPVLVRVHSECLTGDVFGSLRCDCGEQIAKALMKIEEEGKGVLVYMRQEGRGIGLLNKLKAYALQDEGMDTIE
ANLALGFPEDLRDYGIGAQILKDLGIKNIKLMTNNPKKISGLSGYGIKIVDRVPIDMGCKKENRNYLRTKKDKMGHLIDI
M

Sequences:

>Translated_401_residues
MSYKFNTIEEAIEDIKDGKIIVVVDDEDRENEGDLLMAAEKITPEAINFMAKEGRGLICTPVKKSRLEELEICQMVHKNK
DNFGTAFTVSVDAVGTSTGISAFDRAFTVSKIVDLNSKPEDFLKPGHVFPLAAKDGGVLVRAGHTEAAVDMARLSGFSEV
GVICEIMNEDGTMARVPQLMEFVKKHNLKIITIADLIEYRSKEEKLIKALEPVKLPSKYGDFIAIGYENLITGEEHIALI
KGDVKGEDPVLVRVHSECLTGDVFGSLRCDCGEQIAKALMKIEEEGKGVLVYMRQEGRGIGLLNKLKAYALQDEGMDTIE
ANLALGFPEDLRDYGIGAQILKDLGIKNIKLMTNNPKKISGLSGYGIKIVDRVPIDMGCKKENRNYLRTKKDKMGHLIDI
M
>Mature_400_residues
SYKFNTIEEAIEDIKDGKIIVVVDDEDRENEGDLLMAAEKITPEAINFMAKEGRGLICTPVKKSRLEELEICQMVHKNKD
NFGTAFTVSVDAVGTSTGISAFDRAFTVSKIVDLNSKPEDFLKPGHVFPLAAKDGGVLVRAGHTEAAVDMARLSGFSEVG
VICEIMNEDGTMARVPQLMEFVKKHNLKIITIADLIEYRSKEEKLIKALEPVKLPSKYGDFIAIGYENLITGEEHIALIK
GDVKGEDPVLVRVHSECLTGDVFGSLRCDCGEQIAKALMKIEEEGKGVLVYMRQEGRGIGLLNKLKAYALQDEGMDTIEA
NLALGFPEDLRDYGIGAQILKDLGIKNIKLMTNNPKKISGLSGYGIKIVDRVPIDMGCKKENRNYLRTKKDKMGHLIDIM

Specific function: Catalyzes the conversion of GTP to 2,5-diamino-6- ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate [H]

COG id: COG0108

COG function: function code H; 3,4-dihydroxy-2-butanone 4-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the GTP cyclohydrolase II family [H]

Homologues:

Organism=Escherichia coli, GI1787533, Length=193, Percent_Identity=55.440414507772, Blast_Score=235, Evalue=5e-63,
Organism=Escherichia coli, GI1789420, Length=202, Percent_Identity=45.049504950495, Blast_Score=204, Evalue=5e-54,
Organism=Saccharomyces cerevisiae, GI6320695, Length=207, Percent_Identity=44.4444444444444, Blast_Score=180, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6319438, Length=173, Percent_Identity=49.7109826589595, Blast_Score=147, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017945
- InterPro:   IPR000422
- InterPro:   IPR000926
- InterPro:   IPR016299 [H]

Pfam domain/function: PF00926 DHBP_synthase; PF00925 GTP_cyclohydro2 [H]

EC number: =4.1.99.12; =3.5.4.25 [H]

Molecular weight: Translated: 44284; Mature: 44153

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYKFNTIEEAIEDIKDGKIIVVVDDEDRENEGDLLMAAEKITPEAINFMAKEGRGLICT
CCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEHHCCHHHHHHHHHCCCCEEEC
PVKKSRLEELEICQMVHKNKDNFGTAFTVSVDAVGTSTGISAFDRAFTVSKIVDLNSKPE
CCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCH
DFLKPGHVFPLAAKDGGVLVRAGHTEAAVDMARLSGFSEVGVICEIMNEDGTMARVPQLM
HHCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHCCCCHHCEEEEEECCCCCHHHHHHHH
EFVKKHNLKIITIADLIEYRSKEEKLIKALEPVKLPSKYGDFIAIGYENLITGEEHIALI
HHHHHCCCEEEEHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEECHHHHCCCCCEEEEE
KGDVKGEDPVLVRVHSECLTGDVFGSLRCDCGEQIAKALMKIEEEGKGVLVYMRQEGRGI
ECCCCCCCCEEEEEECHHHCCHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
GLLNKLKAYALQDEGMDTIEANLALGFPEDLRDYGIGAQILKDLGIKNIKLMTNNPKKIS
CHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHCCCHHHHHHHCCCCEEEEEECCCCCCC
GLSGYGIKIVDRVPIDMGCKKENRNYLRTKKDKMGHLIDIM
CCCCCCEEEEEECCCCCCCCCCCCCHHHCCHHHCCCEEECC
>Mature Secondary Structure 
SYKFNTIEEAIEDIKDGKIIVVVDDEDRENEGDLLMAAEKITPEAINFMAKEGRGLICT
CCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEHHCCHHHHHHHHHCCCCEEEC
PVKKSRLEELEICQMVHKNKDNFGTAFTVSVDAVGTSTGISAFDRAFTVSKIVDLNSKPE
CCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCH
DFLKPGHVFPLAAKDGGVLVRAGHTEAAVDMARLSGFSEVGVICEIMNEDGTMARVPQLM
HHCCCCCEEEEEECCCCEEEECCCCHHHHHHHHHCCCCHHCEEEEEECCCCCHHHHHHHH
EFVKKHNLKIITIADLIEYRSKEEKLIKALEPVKLPSKYGDFIAIGYENLITGEEHIALI
HHHHHCCCEEEEHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEECHHHHCCCCCEEEEE
KGDVKGEDPVLVRVHSECLTGDVFGSLRCDCGEQIAKALMKIEEEGKGVLVYMRQEGRGI
ECCCCCCCCEEEEEECHHHCCHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
GLLNKLKAYALQDEGMDTIEANLALGFPEDLRDYGIGAQILKDLGIKNIKLMTNNPKKIS
CHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHCCCHHHHHHHCCCCEEEEEECCCCCCC
GLSGYGIKIVDRVPIDMGCKKENRNYLRTKKDKMGHLIDIM
CCCCCCEEEEEECCCCCCCCCCCCCHHHCCHHHCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA