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The map label for this gene is ribH

Identifier: 226950297

GI number: 226950297

Start: 3281038

End: 3281502

Strand: Reverse

Name: ribH

Synonym: CLM_3259

Alternate gene names: 226950297

Gene position: 3281502-3281038 (Counterclockwise)

Preceding gene: 226950298

Following gene: 226950294

Centisome position: 78.97

GC content: 35.05

Gene sequence:

>465_bases
ATGAAAATATATGAAGGTAGATTAACAGCAGAAGGTTTAAAGGTAGGAATAATAGTTTCAAGATTTAATGAGTTTATAAC
ATCAAAATTATTAGCTGGATCTATAGATTGTCTAAAAAGACATGGCGCTAAAGAGGATAATATAGAAGTATGTTGGGTAC
CTGGTGCTTTTGAAATACCTGTAATAGCCAAGAAAATGGCATCAAAGGGTAAATATGATGCAGTAATATGTTTAGGAGCA
GTAATAAGAGGGGCAACGCCTCATTTTGACTATGTATCTAGTGAAGTTTCAAAGGGAGTGGCTCATGTATCATTGGATAA
AGAAGTGCCAGTAATATTTGGAGTATTAACTACAGATACCATAGAGCAAGCCATAGAAAGAGCAGGTACAAAGGCAGGAA
ATAAAGGCTACGATGCGGCTATGTCAGCTATAGAAATGTCAAACTTAATGAAAGTCTTAGATTAG

Upstream 100 bases:

>100_bases
AAAAAAGAAAATCGTAATTATTTAAGAACTAAGAAAGATAAAATGGGACACTTGATAGACATAATGTAGATAAATTAATA
AAAGATTAGGGGGAAATAAA

Downstream 100 bases:

>100_bases
AAATTTATGAATTTGTATAAACAGAGTTCTTGGCTTCAGAGGGATTTTTTACTCCCACTGAAGCTTATTAACAGAATTCT
AAGTAGTTTTACTCCTTAGA

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MKIYEGRLTAEGLKVGIIVSRFNEFITSKLLAGSIDCLKRHGAKEDNIEVCWVPGAFEIPVIAKKMASKGKYDAVICLGA
VIRGATPHFDYVSSEVSKGVAHVSLDKEVPVIFGVLTTDTIEQAIERAGTKAGNKGYDAAMSAIEMSNLMKVLD

Sequences:

>Translated_154_residues
MKIYEGRLTAEGLKVGIIVSRFNEFITSKLLAGSIDCLKRHGAKEDNIEVCWVPGAFEIPVIAKKMASKGKYDAVICLGA
VIRGATPHFDYVSSEVSKGVAHVSLDKEVPVIFGVLTTDTIEQAIERAGTKAGNKGYDAAMSAIEMSNLMKVLD
>Mature_154_residues
MKIYEGRLTAEGLKVGIIVSRFNEFITSKLLAGSIDCLKRHGAKEDNIEVCWVPGAFEIPVIAKKMASKGKYDAVICLGA
VIRGATPHFDYVSSEVSKGVAHVSLDKEVPVIFGVLTTDTIEQAIERAGTKAGNKGYDAAMSAIEMSNLMKVLD

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family

Homologues:

Organism=Escherichia coli, GI1786617, Length=154, Percent_Identity=57.1428571428571, Blast_Score=186, Evalue=9e-49,
Organism=Saccharomyces cerevisiae, GI6324429, Length=155, Percent_Identity=35.4838709677419, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RISB_CLOB1 (A7FXC4)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385128.1
- ProteinModelPortal:   A7FXC4
- SMR:   A7FXC4
- STRING:   A7FXC4
- GeneID:   5396004
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_2829
- eggNOG:   COG0054
- HOGENOM:   HBG311126
- OMA:   KAGNKGW
- ProtClustDB:   PRK00061
- BioCyc:   CBOT441770:CLB_2829-MONOMER
- HAMAP:   MF_00178
- InterPro:   IPR002180
- Gene3D:   G3DSA:3.40.50.960
- PANTHER:   PTHR21058
- TIGRFAMs:   TIGR00114

Pfam domain/function: PF00885 DMRL_synthase; SSF52121 DMRL_synthase

EC number: =2.5.1.9

Molecular weight: Translated: 16576; Mature: 16576

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYEGRLTAEGLKVGIIVSRFNEFITSKLLAGSIDCLKRHGAKEDNIEVCWVPGAFEIP
CCEECCCEECCCEEHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCEEEEEECCCCCCH
VIAKKMASKGKYDAVICLGAVIRGATPHFDYVSSEVSKGVAHVSLDKEVPVIFGVLTTDT
HHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEECCCCCCEEEEHHHHHH
IEQAIERAGTKAGNKGYDAAMSAIEMSNLMKVLD
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MKIYEGRLTAEGLKVGIIVSRFNEFITSKLLAGSIDCLKRHGAKEDNIEVCWVPGAFEIP
CCEECCCEECCCEEHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCEEEEEECCCCCCH
VIAKKMASKGKYDAVICLGAVIRGATPHFDYVSSEVSKGVAHVSLDKEVPVIFGVLTTDT
HHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEEECCCCCCEEEEHHHHHH
IEQAIERAGTKAGNKGYDAAMSAIEMSNLMKVLD
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA