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The map label for this gene is ptbA [H]

Identifier: 226950246

GI number: 226950246

Start: 3223659

End: 3224138

Strand: Reverse

Name: ptbA [H]

Synonym: CLM_3201

Alternate gene names: 226950246

Gene position: 3224138-3223659 (Counterclockwise)

Preceding gene: 226950249

Following gene: 226950244

Centisome position: 77.59

GC content: 31.04

Gene sequence:

>480_bases
ATGTTTGGTTTTTTTAATAGAGAACATGAGATTCTTGCTCCAGCTGCAGGGAAAGTATTAGAATTATCCGAAGTGCCAGA
TGAAGTATTTGCAAGTAAATTAGCAGGAGATGGTGTCGCTATTGATTGTGAAGATGATATTATTGTGGCTCCAGCAGATG
GAGTAATTTCTTTAATATTTAAAACTAATCATGCTTTTGGAATTATATTAAAAGATGGAACAGAGCTTTTAGTTCATATT
GGTATTGACACTGTTAAATTAGAAGGCAAAGGATTTGAAAGATTATTACAGGAAGGAGATAAGGTCAAAGTAGGAGACCC
TATTATTAAGATAGATAGGGAGTTTATTAAATCTCAAGGATATTCTTTGATTACACCTGTGTTAATAACAAATCCTGATA
ATGTTAAAAATATAGAATATAAAACAGGTTTTAATGCTAAGCCAGGAAAGGATATTTTGATTAGCTATACTGATAAATAA

Upstream 100 bases:

>100_bases
AAATCAGGCATGAGTTAGGGATAACTTATGCCTTTTTTTTATTTGAATTTTTATAAAATGTAGGCAAATCTGTATTACCT
ATACTTAGGAGGTTTTAAAA

Downstream 100 bases:

>100_bases
TTTATAAACTTATTTATAATGTTAAATATAAAGTTTATTTATTAAGGCGTAATACTAATATAATTTATGGATATTGGTAT
TACGCCTTTTGGTATAAAAA

Product: PTS system glucose subfamily transporter subunit IIA

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Glc; EIII-Glc; PTS system glucose-specific EIIA component [H]

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MFGFFNREHEILAPAAGKVLELSEVPDEVFASKLAGDGVAIDCEDDIIVAPADGVISLIFKTNHAFGIILKDGTELLVHI
GIDTVKLEGKGFERLLQEGDKVKVGDPIIKIDREFIKSQGYSLITPVLITNPDNVKNIEYKTGFNAKPGKDILISYTDK

Sequences:

>Translated_159_residues
MFGFFNREHEILAPAAGKVLELSEVPDEVFASKLAGDGVAIDCEDDIIVAPADGVISLIFKTNHAFGIILKDGTELLVHI
GIDTVKLEGKGFERLLQEGDKVKVGDPIIKIDREFIKSQGYSLITPVLITNPDNVKNIEYKTGFNAKPGKDILISYTDK
>Mature_159_residues
MFGFFNREHEILAPAAGKVLELSEVPDEVFASKLAGDGVAIDCEDDIIVAPADGVISLIFKTNHAFGIILKDGTELLVHI
GIDTVKLEGKGFERLLQEGDKVKVGDPIIKIDREFIKSQGYSLITPVLITNPDNVKNIEYKTGFNAKPGKDILISYTDK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG2190

COG function: function code G; Phosphotransferase system IIA components

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=129, Percent_Identity=48.062015503876, Blast_Score=134, Evalue=2e-33,
Organism=Escherichia coli, GI1788757, Length=129, Percent_Identity=48.8372093023256, Blast_Score=130, Evalue=5e-32,
Organism=Escherichia coli, GI1790159, Length=128, Percent_Identity=43.75, Blast_Score=106, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR001127 [H]

Pfam domain/function: PF00358 PTS_EIIA_1 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 17371; Mature: 17371

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00371 PTS_EIIA_TYPE_1_HIS ; PS51093 PTS_EIIA_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGFFNREHEILAPAAGKVLELSEVPDEVFASKLAGDGVAIDCEDDIIVAPADGVISLIF
CCCCCCCCCEEECCCCCCEEECCCCCHHHHHHHHCCCCEEEECCCCEEEECCCCEEEEEE
KTNHAFGIILKDGTELLVHIGIDTVKLEGKGFERLLQEGDKVKVGDPIIKIDREFIKSQG
ECCCEEEEEEECCCEEEEEECCEEEEECCCCHHHHHHCCCEEEECCCEEEECHHHHHCCC
YSLITPVLITNPDNVKNIEYKTGFNAKPGKDILISYTDK
CEEEEEEEEECCCCCCEEEEECCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MFGFFNREHEILAPAAGKVLELSEVPDEVFASKLAGDGVAIDCEDDIIVAPADGVISLIF
CCCCCCCCCEEECCCCCCEEECCCCCHHHHHHHHCCCCEEEECCCCEEEECCCCEEEEEE
KTNHAFGIILKDGTELLVHIGIDTVKLEGKGFERLLQEGDKVKVGDPIIKIDREFIKSQG
ECCCEEEEEEECCCEEEEEECCEEEEECCCCHHHHHHCCCEEEECCCEEEECHHHHHCCC
YSLITPVLITNPDNVKNIEYKTGFNAKPGKDILISYTDK
CEEEEEEEEECCCCCCEEEEECCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]