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The map label for this gene is pflC [H]

Identifier: 226949400

GI number: 226949400

Start: 2397761

End: 2398714

Strand: Reverse

Name: pflC [H]

Synonym: CLM_2325

Alternate gene names: 226949400

Gene position: 2398714-2397761 (Counterclockwise)

Preceding gene: 226949401

Following gene: 226949399

Centisome position: 57.73

GC content: 29.98

Gene sequence:

>954_bases
ATGAGTAATGGAAATTTAGGTATAATCGAGAGAAAAGCAAGGATTTTTAATATACAAAAATATAATATGTATGATGGAGA
TGGAATAAGAACTTTAGTATTTTTTCAAGGATGTCCACTTAGATGTAAATGGTGTGCAAATCCTGAAGGACTAGAAAAAA
AATATAGAGTTATGCTTAAAAGTAATCTATGTGTAAATTGTGGAGCTTGTGTTTCTGCATGTCCTGTAGGAATTCATACT
ATTTCTAATAAAACTCTAAAGCATGAAGTTAATCGTGACATAGATTGTATAGGATGTGGAAAGTGTAAAGAAGCTTGTCT
TAAATCTGCTATATCAATAGTTGGAGAAGAAAAAACTATCTCTGAACTTTTAAAAATAGTAGAAGAAGATAGAACCTTTT
ATGAAATGTCCGGCGGTGGAGTTACATTAGGTGGTGGCGAAGTTTTAATGCAGCCTGAAGCTGCTACTAGTTTACTAATG
GCATGTAAGCAAGAAGGTATAAACACTGCAATTGAAACTTGTGGCTATACAAAGCTTGAAACAATCCTTAAGGTTGCAGA
ATTTGTAGACTTATTCTTATTTGATATTAAAAATATTAATTCTGATAGACATCATGAATTAACTGGAGTAAGAAATGAGA
GGATTTTAGAAAATCTTCAAGAATTACTCAAGAAAAAATACAATGTAAAGATTCGTATGCCTTTACTTAAAGGTATAAAT
GATTCTCAAGATGAGATAGAAAAAACTATGGAATTTTTATTACCTTATAAAGATTATAAAAACTTTAAGGGAATTGATTT
ACTTCCTTATCATAAGATGGGGGTAAATAAATATAACCAATTAGGAATGGAATATCCTATAAAAGATGATCCAAGCCTTA
AAAGTGAAGATTTAGATAGAATAGAAGGATGGATTAAAAAATATGATTTACCTGTAAAGGTAATCCGCCATTAA

Upstream 100 bases:

>100_bases
TTATATAAAGTCTGCAATCTACAGCCTGTTTTGTAGATTGCAGATGTAAATTAAGATATATATTAAAAATCAAAGTAAAT
GAAACTGGAGAATGAGTAAT

Downstream 100 bases:

>100_bases
ATAATTTTCAAATAGATAATAAGATGGAAGGTAAGGTTTATGGGAGATTTTTCTGATAGAAATATACAGCGTGTTATACA
AGAGTCAGTGCCAGGAAAGC

Product: glycyl-radical enzyme activating family protein

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 2; PFL-activating enzyme 2 [H]

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MSNGNLGIIERKARIFNIQKYNMYDGDGIRTLVFFQGCPLRCKWCANPEGLEKKYRVMLKSNLCVNCGACVSACPVGIHT
ISNKTLKHEVNRDIDCIGCGKCKEACLKSAISIVGEEKTISELLKIVEEDRTFYEMSGGGVTLGGGEVLMQPEAATSLLM
ACKQEGINTAIETCGYTKLETILKVAEFVDLFLFDIKNINSDRHHELTGVRNERILENLQELLKKKYNVKIRMPLLKGIN
DSQDEIEKTMEFLLPYKDYKNFKGIDLLPYHKMGVNKYNQLGMEYPIKDDPSLKSEDLDRIEGWIKKYDLPVKVIRH

Sequences:

>Translated_317_residues
MSNGNLGIIERKARIFNIQKYNMYDGDGIRTLVFFQGCPLRCKWCANPEGLEKKYRVMLKSNLCVNCGACVSACPVGIHT
ISNKTLKHEVNRDIDCIGCGKCKEACLKSAISIVGEEKTISELLKIVEEDRTFYEMSGGGVTLGGGEVLMQPEAATSLLM
ACKQEGINTAIETCGYTKLETILKVAEFVDLFLFDIKNINSDRHHELTGVRNERILENLQELLKKKYNVKIRMPLLKGIN
DSQDEIEKTMEFLLPYKDYKNFKGIDLLPYHKMGVNKYNQLGMEYPIKDDPSLKSEDLDRIEGWIKKYDLPVKVIRH
>Mature_316_residues
SNGNLGIIERKARIFNIQKYNMYDGDGIRTLVFFQGCPLRCKWCANPEGLEKKYRVMLKSNLCVNCGACVSACPVGIHTI
SNKTLKHEVNRDIDCIGCGKCKEACLKSAISIVGEEKTISELLKIVEEDRTFYEMSGGGVTLGGGEVLMQPEAATSLLMA
CKQEGINTAIETCGYTKLETILKVAEFVDLFLFDIKNINSDRHHELTGVRNERILENLQELLKKKYNVKIRMPLLKGIND
SQDEIEKTMEFLLPYKDYKNFKGIDLLPYHKMGVNKYNQLGMEYPIKDDPSLKSEDLDRIEGWIKKYDLPVKVIRH

Specific function: Activation of pyruvate formate-lyase 2 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI1790389, Length=302, Percent_Identity=34.7682119205298, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI226510931, Length=286, Percent_Identity=36.013986013986, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI1787130, Length=196, Percent_Identity=36.2244897959184, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1790839, Length=271, Percent_Identity=25.830258302583, Blast_Score=85, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006638
- InterPro:   IPR012839
- InterPro:   IPR011352
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 36017; Mature: 35886

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: PS01087 RADICAL_ACTIVATING ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNGNLGIIERKARIFNIQKYNMYDGDGIRTLVFFQGCPLRCKWCANPEGLEKKYRVMLK
CCCCCEEEEECCCEEEEEEEECCCCCCCCEEEEEECCCCCEEEECCCCCHHHHHHHHHHH
SNLCVNCGACVSACPVGIHTISNKTLKHEVNRDIDCIGCGKCKEACLKSAISIVGEEKTI
CCCEEECCHHHHHCCCCEEHHCCCHHHHHHCCCCCEEECCHHHHHHHHHHHHHHCCHHHH
SELLKIVEEDRTFYEMSGGGVTLGGGEVLMQPEAATSLLMACKQEGINTAIETCGYTKLE
HHHHHHHHCCCCEEEECCCEEEECCCCEEECCHHHHHHHHHHHHHCHHHHHHHCCCHHHH
TILKVAEFVDLFLFDIKNINSDRHHELTGVRNERILENLQELLKKKYNVKIRMPLLKGIN
HHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCEEEEEHHHCCCC
DSQDEIEKTMEFLLPYKDYKNFKGIDLLPYHKMGVNKYNQLGMEYPIKDDPSLKSEDLDR
CCHHHHHHHHHHHCCHHHCCCCCCCCCCCCHHHCCCHHHHCCCCCCCCCCCCCCHHHHHH
IEGWIKKYDLPVKVIRH
HHHHHHHCCCCHHHHCC
>Mature Secondary Structure 
SNGNLGIIERKARIFNIQKYNMYDGDGIRTLVFFQGCPLRCKWCANPEGLEKKYRVMLK
CCCCEEEEECCCEEEEEEEECCCCCCCCEEEEEECCCCCEEEECCCCCHHHHHHHHHHH
SNLCVNCGACVSACPVGIHTISNKTLKHEVNRDIDCIGCGKCKEACLKSAISIVGEEKTI
CCCEEECCHHHHHCCCCEEHHCCCHHHHHHCCCCCEEECCHHHHHHHHHHHHHHCCHHHH
SELLKIVEEDRTFYEMSGGGVTLGGGEVLMQPEAATSLLMACKQEGINTAIETCGYTKLE
HHHHHHHHCCCCEEEECCCEEEECCCCEEECCHHHHHHHHHHHHHCHHHHHHHCCCHHHH
TILKVAEFVDLFLFDIKNINSDRHHELTGVRNERILENLQELLKKKYNVKIRMPLLKGIN
HHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHCCEEEEEHHHCCCC
DSQDEIEKTMEFLLPYKDYKNFKGIDLLPYHKMGVNKYNQLGMEYPIKDDPSLKSEDLDR
CCHHHHHHHHHHHCCHHHCCCCCCCCCCCCHHHCCCHHHHCCCCCCCCCCCCCCHHHHHH
IEGWIKKYDLPVKVIRH
HHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]