| Definition | Clostridium botulinum A2 str. Kyoto chromosome, complete genome. |
|---|---|
| Accession | NC_012563 |
| Length | 4,155,278 |
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The map label for this gene is pflD [H]
Identifier: 226949401
GI number: 226949401
Start: 2398878
End: 2401418
Strand: Reverse
Name: pflD [H]
Synonym: CLM_2326
Alternate gene names: 226949401
Gene position: 2401418-2398878 (Counterclockwise)
Preceding gene: 226949402
Following gene: 226949400
Centisome position: 57.79
GC content: 35.1
Gene sequence:
>2541_bases TTGGATATACGTGAATTTTCAAATAAATTTATGGAAGCTACTAAAAATATGTCTGATGAAGAACGCGCTGGATTAATGAA GATGTTTCAAAGTGTTTCTAATGAAATAACAAGAGAAGAACCAGCTACATCAAAGGTTGCGTGTGATAATAATGGTGAAA TACCAGATGGAATGACAGAGCGTCTAGTTAAGTTAAAGGAAACTTATCTAAAACATGTTCCAACAATAACAACTCATAGA GCAAGAGCTATTACTAAAATAGCAAAAGAAAACCCTGGTACTCCTAAATCAGTTTTACGTGGTAAATGTTTTAAACATTG TTGCGAAACTGCACCACTTGTAATTCAAGACAATGAACTTATAGTTGGAGCACCAAATGGACAACCTCGTGCAGGAGCAT TTTCTCCTGATATAGCTTGGAGATGGATGGTTGATGAAATTGATACAATAGGAACTCGTCCACAAGATCCATTCTATATA TCAGAAGAAGATAAGAAAATCATGCGTGAGGAGTTATTCCCATATTGGGCGGGTAAATCCGTTGATGAATACTGCGAAGA TCAATATCGTGAAGCAGGAGTATGGGAACTTTCAGGAGAATCTTTTGTTTCAGATTGTTCATACCACGCAGTAAATGGTG GAGGAGACTCTAACCCAGGATATGACGTTGTATTAATGAAAAAAGGTATGCTTGATATAAAGAGAGAAGCAGAAGAAAAA TTAGCTGAACTTAAATATGAAAATCCAGAGGATATAGATAAAATCTATTTCTACAAATCATTAATTGATACCGCTGAAGG TGTTATGATATATGCTAAACGTATGTCAGATTATGCTGCTGAATTAGCTCAAAAAGAAACTAACCCTAAGCGTAAAGCAG AACTTCTAAAGATTTCTGAAATAAATGCTAGAGTTCCAGCACATAAGCCAAGTACTTATTGGGAAGCAATTCAAGCAGTT TGGACTATAGAATCATTACTTGTAGTTGAGGAAAATCAAACAGGTATGTCTATAGGACGTGTTGACCAATACATGTACCC ATTCTACAAAGCTGATATTGAAGCTGGACGTATGACTGATTATGAAGCATTTGAATTATCAGGTTGTATGCTTATAAAAA TGTCTGAAATGATGTGGATAACAAGTGAAGGTGGTTCTAAATTCTTCGCAGGTTATCAACCATTTGTAAATATGTGTGTA GGTGGTGTTACTAGAGAAGGCCGTGATGCTACAAACGAATTAACATACCTTTTAATGGATGCAGTTCGTCATGTTAAAAT ATATCAACCATCTTTAGCTTGCCGTATACACAAATCATCACCACAAAAATATCTTAAAAAGATAGTTGACGTTATTCGTG CAGGTATGGGATTCCCAGCATGCCACTTTGACGATGTTCATATAAAAATTATGTTAGCTAAAGGTGTTTCTATAGAAGAC GCAAGAGATTACTGCTTAATGGGTTGTGTTGAACCACAAAAATCCGGAAGACTATATCAATGGACTTCTACAGGATACAC TCAATGGCCTATATGTATAGAACTTGTTTTAAACAATGGTGTACCATTATGGTATGGTAAACAAGTATGCCCAGATATGG GAGACTTAAGCCAGTTTAAAACTTATGAACAATTTGAAGCAGCTGTTAAAGAGCAAATCAAATTCATTACTAAATGGACA AGTGTTGCTACAGTAATTTCTCAGCGTGTTCATAAAGAACTTGCTCCAAAGCCACTTATGTCTATGATGTATGAAGGTTG TATGGAAAATGGTAGAGGGGTAGAAGCCGGCGGAGCTATGTATAACTTCGGACCTGGAGTAGTATGGAGTGGACTTGCTA CTTATGCAGATTCCATGGCAGCTATAAAGAAATTAGTATTTGAAGATAAAAAATATACTCTACAAGAAATGAACGAAGCT TTAAAAGCAGATTTCGTTGGATATGAACAATTAAGAAAAGATTGTTTAGAAGCACCTAAGTATGGTAACGATGATGATTA TGCAGATTTAATTGCTGCTGATTTAATTAACTTTACAGAACAAGAACATCGTAAATATAAAACATTATATTCAGTACTTA GTCATGGTACTTTATCAATATCAAACAATACTCCATTTGGACAAATGACTGGAGCTACAGCAAATGGACGTAGAGCATGG ATGCCTTTATCAGATGGTATAAGTCCATCACAAGGCGCTGATTTTAAAGGCCCTACTTCTATAATAAAATCTGTTTCTAA GATGTCTTGTGAAGATATGAATATAGGTATGGTTCATAACTTTAAGTTAATAGCTGGTCTTCTTGATACACCAGAAGGAG AACAAGGAATCATTACATTATTACGTAGTGCTTGTGCCCTTCAACTTGGAGAAGTTCAATTTAACTATTTAGACAACAAG ACTTTAATAGAAGCTCAAAAACATCCAGATCAATATCGTGATTTAATTGTTCGTGTTGCTGGATACAGTGCATTCTTCGT TGAGTTATGTAAAGATGTTCAAGATGAAATTATAAGTAGAACTATGCTTACACATTTCTAA
Upstream 100 bases:
>100_bases TATAAGTTATTAGAGAGTATTTTAAAAAAAGTTCTACAGGACTTAAAATAAAAAATCATATTTAATATATGAAATACATT AAAGAATGTGAGGGAATAAA
Downstream 100 bases:
>100_bases TAAAAATTTGATGGAAAATATATAACTTATAGAAAATATATAACTTAGTCCCTTTAAAATAATTTATATAAAGTCTGCAA TCTACAGCCTGTTTTGTAGA
Product: formate acetyltransferase
Products: NA
Alternate protein names: Pyruvate formate-lyase 2 [H]
Number of amino acids: Translated: 846; Mature: 846
Protein sequence:
>846_residues MDIREFSNKFMEATKNMSDEERAGLMKMFQSVSNEITREEPATSKVACDNNGEIPDGMTERLVKLKETYLKHVPTITTHR ARAITKIAKENPGTPKSVLRGKCFKHCCETAPLVIQDNELIVGAPNGQPRAGAFSPDIAWRWMVDEIDTIGTRPQDPFYI SEEDKKIMREELFPYWAGKSVDEYCEDQYREAGVWELSGESFVSDCSYHAVNGGGDSNPGYDVVLMKKGMLDIKREAEEK LAELKYENPEDIDKIYFYKSLIDTAEGVMIYAKRMSDYAAELAQKETNPKRKAELLKISEINARVPAHKPSTYWEAIQAV WTIESLLVVEENQTGMSIGRVDQYMYPFYKADIEAGRMTDYEAFELSGCMLIKMSEMMWITSEGGSKFFAGYQPFVNMCV GGVTREGRDATNELTYLLMDAVRHVKIYQPSLACRIHKSSPQKYLKKIVDVIRAGMGFPACHFDDVHIKIMLAKGVSIED ARDYCLMGCVEPQKSGRLYQWTSTGYTQWPICIELVLNNGVPLWYGKQVCPDMGDLSQFKTYEQFEAAVKEQIKFITKWT SVATVISQRVHKELAPKPLMSMMYEGCMENGRGVEAGGAMYNFGPGVVWSGLATYADSMAAIKKLVFEDKKYTLQEMNEA LKADFVGYEQLRKDCLEAPKYGNDDDYADLIAADLINFTEQEHRKYKTLYSVLSHGTLSISNNTPFGQMTGATANGRRAW MPLSDGISPSQGADFKGPTSIIKSVSKMSCEDMNIGMVHNFKLIAGLLDTPEGEQGIITLLRSACALQLGEVQFNYLDNK TLIEAQKHPDQYRDLIVRVAGYSAFFVELCKDVQDEIISRTMLTHF
Sequences:
>Translated_846_residues MDIREFSNKFMEATKNMSDEERAGLMKMFQSVSNEITREEPATSKVACDNNGEIPDGMTERLVKLKETYLKHVPTITTHR ARAITKIAKENPGTPKSVLRGKCFKHCCETAPLVIQDNELIVGAPNGQPRAGAFSPDIAWRWMVDEIDTIGTRPQDPFYI SEEDKKIMREELFPYWAGKSVDEYCEDQYREAGVWELSGESFVSDCSYHAVNGGGDSNPGYDVVLMKKGMLDIKREAEEK LAELKYENPEDIDKIYFYKSLIDTAEGVMIYAKRMSDYAAELAQKETNPKRKAELLKISEINARVPAHKPSTYWEAIQAV WTIESLLVVEENQTGMSIGRVDQYMYPFYKADIEAGRMTDYEAFELSGCMLIKMSEMMWITSEGGSKFFAGYQPFVNMCV GGVTREGRDATNELTYLLMDAVRHVKIYQPSLACRIHKSSPQKYLKKIVDVIRAGMGFPACHFDDVHIKIMLAKGVSIED ARDYCLMGCVEPQKSGRLYQWTSTGYTQWPICIELVLNNGVPLWYGKQVCPDMGDLSQFKTYEQFEAAVKEQIKFITKWT SVATVISQRVHKELAPKPLMSMMYEGCMENGRGVEAGGAMYNFGPGVVWSGLATYADSMAAIKKLVFEDKKYTLQEMNEA LKADFVGYEQLRKDCLEAPKYGNDDDYADLIAADLINFTEQEHRKYKTLYSVLSHGTLSISNNTPFGQMTGATANGRRAW MPLSDGISPSQGADFKGPTSIIKSVSKMSCEDMNIGMVHNFKLIAGLLDTPEGEQGIITLLRSACALQLGEVQFNYLDNK TLIEAQKHPDQYRDLIVRVAGYSAFFVELCKDVQDEIISRTMLTHF >Mature_846_residues MDIREFSNKFMEATKNMSDEERAGLMKMFQSVSNEITREEPATSKVACDNNGEIPDGMTERLVKLKETYLKHVPTITTHR ARAITKIAKENPGTPKSVLRGKCFKHCCETAPLVIQDNELIVGAPNGQPRAGAFSPDIAWRWMVDEIDTIGTRPQDPFYI SEEDKKIMREELFPYWAGKSVDEYCEDQYREAGVWELSGESFVSDCSYHAVNGGGDSNPGYDVVLMKKGMLDIKREAEEK LAELKYENPEDIDKIYFYKSLIDTAEGVMIYAKRMSDYAAELAQKETNPKRKAELLKISEINARVPAHKPSTYWEAIQAV WTIESLLVVEENQTGMSIGRVDQYMYPFYKADIEAGRMTDYEAFELSGCMLIKMSEMMWITSEGGSKFFAGYQPFVNMCV GGVTREGRDATNELTYLLMDAVRHVKIYQPSLACRIHKSSPQKYLKKIVDVIRAGMGFPACHFDDVHIKIMLAKGVSIED ARDYCLMGCVEPQKSGRLYQWTSTGYTQWPICIELVLNNGVPLWYGKQVCPDMGDLSQFKTYEQFEAAVKEQIKFITKWT SVATVISQRVHKELAPKPLMSMMYEGCMENGRGVEAGGAMYNFGPGVVWSGLATYADSMAAIKKLVFEDKKYTLQEMNEA LKADFVGYEQLRKDCLEAPKYGNDDDYADLIAADLINFTEQEHRKYKTLYSVLSHGTLSISNNTPFGQMTGATANGRRAW MPLSDGISPSQGADFKGPTSIIKSVSKMSCEDMNIGMVHNFKLIAGLLDTPEGEQGIITLLRSACALQLGEVQFNYLDNK TLIEAQKHPDQYRDLIVRVAGYSAFFVELCKDVQDEIISRTMLTHF
Specific function: Glucose metabolism (nonoxidative conversion). [C]
COG id: COG1882
COG function: function code C; Pyruvate-formate lyase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 pyruvate formate lyase domain [H]
Homologues:
Organism=Escherichia coli, GI1790388, Length=796, Percent_Identity=33.7939698492462, Blast_Score=436, Evalue=1e-123, Organism=Escherichia coli, GI1787044, Length=819, Percent_Identity=32.6007326007326, Blast_Score=406, Evalue=1e-114, Organism=Escherichia coli, GI48994926, Length=591, Percent_Identity=22.8426395939086, Blast_Score=128, Evalue=2e-30, Organism=Escherichia coli, GI1787131, Length=543, Percent_Identity=22.2836095764273, Blast_Score=119, Evalue=8e-28,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001150 - InterPro: IPR019777 - InterPro: IPR004184 - InterPro: IPR010098 [H]
Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]
EC number: =2.3.1.54 [H]
Molecular weight: Translated: 95431; Mature: 95431
Theoretical pI: Translated: 5.19; Mature: 5.19
Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
2.2 %Cys (Translated Protein) 4.5 %Met (Translated Protein) 6.7 %Cys+Met (Translated Protein) 2.2 %Cys (Mature Protein) 4.5 %Met (Mature Protein) 6.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MDIREFSNKFMEATKNMSDEERAGLMKMFQSVSNEITREEPATSKVACDNNGEIPDGMTE CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHH RLVKLKETYLKHVPTITTHRARAITKIAKENPGTPKSVLRGKCFKHCCETAPLVIQDNEL HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEECCEE IVGAPNGQPRAGAFSPDIAWRWMVDEIDTIGTRPQDPFYISEEDKKIMREELFPYWAGKS EEECCCCCCCCCCCCCCCEEEEEHHHHHHCCCCCCCCEEECCHHHHHHHHHHCCCCCCCC VDEYCEDQYREAGVWELSGESFVSDCSYHAVNGGGDSNPGYDVVLMKKGMLDIKREAEEK HHHHHHHHHHHCCCEEECCCHHHHCCCCEEECCCCCCCCCEEEEEEECCCHHHHHHHHHH LAELKYENPEDIDKIYFYKSLIDTAEGVMIYAKRMSDYAAELAQKETNPKRKAELLKISE HHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH INARVPAHKPSTYWEAIQAVWTIESLLVVEENQTGMSIGRVDQYMYPFYKADIEAGRMTD CCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHCCHHHHCCCCCCCCC YEAFELSGCMLIKMSEMMWITSEGGSKFFAGYQPFVNMCVGGVTREGRDATNELTYLLMD CCHHCCCCEEEEEEECEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHH AVRHVKIYQPSLACRIHKSSPQKYLKKIVDVIRAGMGFPACHFDDVHIKIMLAKGVSIED HHHHHEEECCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCC ARDYCLMGCVEPQKSGRLYQWTSTGYTQWPICIELVLNNGVPLWYGKQVCPDMGDLSQFK CCCHHHHCCCCCCCCCCEEEEECCCCCCCCHHEEEECCCCCCEEECHHHCCCCCCHHHHH TYEQFEAAVKEQIKFITKWTSVATVISQRVHKELAPKPLMSMMYEGCMENGRGVEAGGAM HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCE YNFGPGVVWSGLATYADSMAAIKKLVFEDKKYTLQEMNEALKADFVGYEQLRKDCLEAPK EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCC YGNDDDYADLIAADLINFTEQEHRKYKTLYSVLSHGTLSISNNTPFGQMTGATANGRRAW CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCEEE MPLSDGISPSQGADFKGPTSIIKSVSKMSCEDMNIGMVHNFKLIAGLLDTPEGEQGIITL CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHCCCCCCHHHHHH LRSACALQLGEVQFNYLDNKTLIEAQKHPDQYRDLIVRVAGYSAFFVELCKDVQDEIISR HHHHHHHHHCCEEEEECCCCEEEHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH TMLTHF HHHHCC >Mature Secondary Structure MDIREFSNKFMEATKNMSDEERAGLMKMFQSVSNEITREEPATSKVACDNNGEIPDGMTE CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHH RLVKLKETYLKHVPTITTHRARAITKIAKENPGTPKSVLRGKCFKHCCETAPLVIQDNEL HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEECCEE IVGAPNGQPRAGAFSPDIAWRWMVDEIDTIGTRPQDPFYISEEDKKIMREELFPYWAGKS EEECCCCCCCCCCCCCCCEEEEEHHHHHHCCCCCCCCEEECCHHHHHHHHHHCCCCCCCC VDEYCEDQYREAGVWELSGESFVSDCSYHAVNGGGDSNPGYDVVLMKKGMLDIKREAEEK HHHHHHHHHHHCCCEEECCCHHHHCCCCEEECCCCCCCCCEEEEEEECCCHHHHHHHHHH LAELKYENPEDIDKIYFYKSLIDTAEGVMIYAKRMSDYAAELAQKETNPKRKAELLKISE HHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH INARVPAHKPSTYWEAIQAVWTIESLLVVEENQTGMSIGRVDQYMYPFYKADIEAGRMTD CCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHCCHHHHCCCCCCCCC YEAFELSGCMLIKMSEMMWITSEGGSKFFAGYQPFVNMCVGGVTREGRDATNELTYLLMD CCHHCCCCEEEEEEECEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHH AVRHVKIYQPSLACRIHKSSPQKYLKKIVDVIRAGMGFPACHFDDVHIKIMLAKGVSIED HHHHHEEECCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCC ARDYCLMGCVEPQKSGRLYQWTSTGYTQWPICIELVLNNGVPLWYGKQVCPDMGDLSQFK CCCHHHHCCCCCCCCCCEEEEECCCCCCCCHHEEEECCCCCCEEECHHHCCCCCCHHHHH TYEQFEAAVKEQIKFITKWTSVATVISQRVHKELAPKPLMSMMYEGCMENGRGVEAGGAM HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCE YNFGPGVVWSGLATYADSMAAIKKLVFEDKKYTLQEMNEALKADFVGYEQLRKDCLEAPK EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCC YGNDDDYADLIAADLINFTEQEHRKYKTLYSVLSHGTLSISNNTPFGQMTGATANGRRAW CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCEEE MPLSDGISPSQGADFKGPTSIIKSVSKMSCEDMNIGMVHNFKLIAGLLDTPEGEQGIITL CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHCCCCCCHHHHHH LRSACALQLGEVQFNYLDNKTLIEAQKHPDQYRDLIVRVAGYSAFFVELCKDVQDEIISR HHHHHHHHHCCEEEEECCCCEEEHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH TMLTHF HHHHCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8265357; 9278503; 7773398 [H]