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The map label for this gene is cwlM [H]

Identifier: 226949366

GI number: 226949366

Start: 2356294

End: 2356998

Strand: Reverse

Name: cwlM [H]

Synonym: CLM_2289

Alternate gene names: 226949366

Gene position: 2356998-2356294 (Counterclockwise)

Preceding gene: 226949367

Following gene: 226949365

Centisome position: 56.72

GC content: 31.21

Gene sequence:

>705_bases
ATGGCTAGGTTATGCTTTGATTATGGACATGGAGGAGAAGATAGTGGTGCATGTTATAAAGGAAGAAAAGAAAGTAATGA
TGTATTAAGTTTAGGTAAAGCTGTAGCAGCAGAGGTTAGAAGATATGGAGTTACAGTTGATGAAACAAGAATTAAAGATA
TTACAGTAAGTTTAATGCAAAGAAGTAACTTTGAAAACAGAAACAATTATAATTATTTCATATCTTTTCATAGAAATGCT
TTTAAGCCAGAAAAAGCTAGAGGTGCTGAAACTTATATTTATTTAAGTGCAAGTACAAAAGCTAAAGCACTGGCAGAAAA
AATACAAGTTGGACTTGTAAGTATTGGATTTGTTAATAGAGGAGTAAAGACCGCTAACTATCATGTACTAAGAGAAACTA
GATGTCCAGCAGTTTTAATTGAAATTGGATTTATTGATAGTACAAATGATAATAATTTATTTGATGGAAAAAGAAATGAA
ATTATTATAGAAATAGCTAAAGCTATATTATCTCAGCTAGGTATAAGTTATAAAGAAAATAAATCTAATGAAGCAATAGC
TCAGCAAAAGCAGCAAGCCACAAGTGGACAAACTCTTTATAGAGTTATGGCGGGATCTTATACGGTAAGGGAAAATGCTG
AAAATCAAGTGCAGAGGTTAAAGGTATCAGGTTTTGATGCAACGATTATGGTATGTAATAGATAA

Upstream 100 bases:

>100_bases
AATGAAGGAATCAGTATCTTAGAAAATACAGCAAAGATAGGCCTACCAATACCTGAAAGATTAAAAAATATTTTAGAGCA
GTTAAAGGAGAAAAATAAAA

Downstream 100 bases:

>100_bases
TTCAAGTGGCAAAAATCATATTTAGTATAATAACCTTCATAAGTATAATATTGAGTGTAAGTACTTATTACATTGATAAA
ATTAATTTTAGGGAGGTGCT

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MARLCFDYGHGGEDSGACYKGRKESNDVLSLGKAVAAEVRRYGVTVDETRIKDITVSLMQRSNFENRNNYNYFISFHRNA
FKPEKARGAETYIYLSASTKAKALAEKIQVGLVSIGFVNRGVKTANYHVLRETRCPAVLIEIGFIDSTNDNNLFDGKRNE
IIIEIAKAILSQLGISYKENKSNEAIAQQKQQATSGQTLYRVMAGSYTVRENAENQVQRLKVSGFDATIMVCNR

Sequences:

>Translated_234_residues
MARLCFDYGHGGEDSGACYKGRKESNDVLSLGKAVAAEVRRYGVTVDETRIKDITVSLMQRSNFENRNNYNYFISFHRNA
FKPEKARGAETYIYLSASTKAKALAEKIQVGLVSIGFVNRGVKTANYHVLRETRCPAVLIEIGFIDSTNDNNLFDGKRNE
IIIEIAKAILSQLGISYKENKSNEAIAQQKQQATSGQTLYRVMAGSYTVRENAENQVQRLKVSGFDATIMVCNR
>Mature_233_residues
ARLCFDYGHGGEDSGACYKGRKESNDVLSLGKAVAAEVRRYGVTVDETRIKDITVSLMQRSNFENRNNYNYFISFHRNAF
KPEKARGAETYIYLSASTKAKALAEKIQVGLVSIGFVNRGVKTANYHVLRETRCPAVLIEIGFIDSTNDNNLFDGKRNEI
IIEIAKAILSQLGISYKENKSNEAIAQQKQQATSGQTLYRVMAGSYTVRENAENQVQRLKVSGFDATIMVCNR

Specific function: Hydrolyzes the cell wall of M.luteus more efficiently than that of B.licheniformis and B.subtilis. The C-terminal region, including the repeats, determines substrate specificity [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Probable). Note=Accumulates in cells as inclusion bodies. May be secreted by a mechanism different from the normal export system [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR007730 [H]

Pfam domain/function: PF01520 Amidase_3; PF05036 SPOR [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 26157; Mature: 26026

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLCFDYGHGGEDSGACYKGRKESNDVLSLGKAVAAEVRRYGVTVDETRIKDITVSLMQ
CCEEEEECCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RSNFENRNNYNYFISFHRNAFKPEKARGAETYIYLSASTKAKALAEKIQVGLVSIGFVNR
HCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHEEEEEECCC
GVKTANYHVLRETRCPAVLIEIGFIDSTNDNNLFDGKRNEIIIEIAKAILSQLGISYKEN
CCCCCCEEEEECCCCCEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
KSNEAIAQQKQQATSGQTLYRVMAGSYTVRENAENQVQRLKVSGFDATIMVCNR
CCHHHHHHHHHHHCCCHHHHHHHHCCEEECCCHHHHHHEEEECCCCEEEEEECC
>Mature Secondary Structure 
ARLCFDYGHGGEDSGACYKGRKESNDVLSLGKAVAAEVRRYGVTVDETRIKDITVSLMQ
CEEEEECCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
RSNFENRNNYNYFISFHRNAFKPEKARGAETYIYLSASTKAKALAEKIQVGLVSIGFVNR
HCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHEEEEEECCC
GVKTANYHVLRETRCPAVLIEIGFIDSTNDNNLFDGKRNEIIIEIAKAILSQLGISYKEN
CCCCCCEEEEECCCCCEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
KSNEAIAQQKQQATSGQTLYRVMAGSYTVRENAENQVQRLKVSGFDATIMVCNR
CCHHHHHHHHHHHCCCHHHHHHHHCCEEECCCHHHHHHEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1495475 [H]