Click here to switch to the map view.

The map label for this gene is rfbB [H]

Identifier: 226949349

GI number: 226949349

Start: 2342766

End: 2343746

Strand: Direct

Name: rfbB [H]

Synonym: CLM_2272

Alternate gene names: 226949349

Gene position: 2342766-2343746 (Clockwise)

Preceding gene: 226949348

Following gene: 226949350

Centisome position: 56.38

GC content: 37.1

Gene sequence:

>981_bases
ATGAGAGTTTTACTTGTAACAGGCGGTGCAGGCTTTATTGGCAGCAATTTTATACGATACTTTCTGGAAACAAACAAAGA
CTTTATTGTAGTTAATTATGATAAGCTTACCTATGCTGGAAACCTTAATAATTTAAAGGAAGTTGAAATGCTTCCGACTT
ATCGCTTTGTTAAGGGCGATATATGTGACAGCGAGGAATTCAATAGGACATTGAAGAAGTATAACCCTGACTATATAATC
AATTTTGCTGCTGAATCGCATGTAGATAGAAGTATAAGTGGACCTTCAGTATTTGGTCAAACGAACTTTATGGGAACACT
TAATTTACTCCAGTGTGCTCATGAATTTTGGGGTAGCAGCTGTGCGGACAAGCGTTTTTTGCAGGTCTCAACGGATGAGG
TATATGGAAGTATTGAGAATGACAGCGATTATTTTATAGAAGATTCAAATCTCATGCCTAACAGTCCTTATTCAGCCTCA
AAGGCAGGAGCAGATATGATGGTAAGAGCCTTTGGGAGAACTTACGGACTTCCTGTTATAATTACAAGATGCTGTAATAA
TTATGGACCGTACCAATACAGTGAGAAGCTTATTCCTATCTGTATTATAAAAGCTTTAAATGATGAGCCTATACCGATAT
ATGGTGATGGCACAAATATAAGAGAATGGATTCATGTATCAGATCACTGTTCTGCAATTATAAAAGCGTTATTTTATGGT
ACGCCAGGAGAAGTTTACAATATTGGAAGTGGAGAAGAGGTTTCAAATGTTGATATGGCAAAGATAATTCTTAGTAATCT
TAGCAAACCAACGGATGCCATAAAAAAAGTGAATGACAGATTGGGCCATGACAGACGGTATGCGTTGGATAGCAGTAAAA
TAAAAAACCAGTTAAGCTGGGCTTGCAGCTATACGCTGGAAGAAGGAATCAAGGAAACAATTGAATGGTATAAAAATAAC
CAGACCTGGTGGAATGATTAA

Upstream 100 bases:

>100_bases
ATAAGAGACCGTTTTCTCATACTTTCCTTTTAGGTGATGATAGTGAAATTGATCTTTAGAGAAAATTAAATATAAATTCA
TGATTGGAGGAAGTTTTTAA

Downstream 100 bases:

>100_bases
TATTTAAATTAGCAAGTAAGAAATCAATTCATTAATTGCTACTTGACCTTAGAGTATTCAAAATTTAGAGAATTTTATTA
TTTTCATTGCTTAGAGTATA

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MRVLLVTGGAGFIGSNFIRYFLETNKDFIVVNYDKLTYAGNLNNLKEVEMLPTYRFVKGDICDSEEFNRTLKKYNPDYII
NFAAESHVDRSISGPSVFGQTNFMGTLNLLQCAHEFWGSSCADKRFLQVSTDEVYGSIENDSDYFIEDSNLMPNSPYSAS
KAGADMMVRAFGRTYGLPVIITRCCNNYGPYQYSEKLIPICIIKALNDEPIPIYGDGTNIREWIHVSDHCSAIIKALFYG
TPGEVYNIGSGEEVSNVDMAKIILSNLSKPTDAIKKVNDRLGHDRRYALDSSKIKNQLSWACSYTLEEGIKETIEWYKNN
QTWWND

Sequences:

>Translated_326_residues
MRVLLVTGGAGFIGSNFIRYFLETNKDFIVVNYDKLTYAGNLNNLKEVEMLPTYRFVKGDICDSEEFNRTLKKYNPDYII
NFAAESHVDRSISGPSVFGQTNFMGTLNLLQCAHEFWGSSCADKRFLQVSTDEVYGSIENDSDYFIEDSNLMPNSPYSAS
KAGADMMVRAFGRTYGLPVIITRCCNNYGPYQYSEKLIPICIIKALNDEPIPIYGDGTNIREWIHVSDHCSAIIKALFYG
TPGEVYNIGSGEEVSNVDMAKIILSNLSKPTDAIKKVNDRLGHDRRYALDSSKIKNQLSWACSYTLEEGIKETIEWYKNN
QTWWND
>Mature_326_residues
MRVLLVTGGAGFIGSNFIRYFLETNKDFIVVNYDKLTYAGNLNNLKEVEMLPTYRFVKGDICDSEEFNRTLKKYNPDYII
NFAAESHVDRSISGPSVFGQTNFMGTLNLLQCAHEFWGSSCADKRFLQVSTDEVYGSIENDSDYFIEDSNLMPNSPYSAS
KAGADMMVRAFGRTYGLPVIITRCCNNYGPYQYSEKLIPICIIKALNDEPIPIYGDGTNIREWIHVSDHCSAIIKALFYG
TPGEVYNIGSGEEVSNVDMAKIILSNLSKPTDAIKKVNDRLGHDRRYALDSSKIKNQLSWACSYTLEEGIKETIEWYKNN
QTWWND

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=333, Percent_Identity=38.4384384384384, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI42516563, Length=320, Percent_Identity=25.625, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI56237023, Length=344, Percent_Identity=25.5813953488372, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI56118217, Length=344, Percent_Identity=25.5813953488372, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI189083684, Length=344, Percent_Identity=25.5813953488372, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI48994969, Length=340, Percent_Identity=47.0588235294118, Blast_Score=322, Evalue=1e-89,
Organism=Escherichia coli, GI1788353, Length=339, Percent_Identity=45.7227138643068, Blast_Score=305, Evalue=2e-84,
Organism=Escherichia coli, GI1786974, Length=355, Percent_Identity=26.4788732394366, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788366, Length=353, Percent_Identity=24.0793201133144, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17568069, Length=322, Percent_Identity=38.5093167701863, Blast_Score=218, Evalue=5e-57,
Organism=Caenorhabditis elegans, GI115532424, Length=321, Percent_Identity=30.8411214953271, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17539532, Length=328, Percent_Identity=25.9146341463415, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71982035, Length=349, Percent_Identity=26.9340974212034, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI133901786, Length=346, Percent_Identity=22.5433526011561, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI133901790, Length=346, Percent_Identity=22.5433526011561, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17539422, Length=346, Percent_Identity=22.5433526011561, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI133901788, Length=346, Percent_Identity=22.5433526011561, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17539424, Length=350, Percent_Identity=22.5714285714286, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=353, Percent_Identity=26.0623229461756, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21356223, Length=327, Percent_Identity=25.3822629969419, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 36950; Mature: 36950

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLLVTGGAGFIGSNFIRYFLETNKDFIVVNYDKLTYAGNLNNLKEVEMLPTYRFVKGD
CEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCEEEECCCCCCHHHHHCCCCCEEECC
ICDSEEFNRTLKKYNPDYIINFAAESHVDRSISGPSVFGQTNFMGTLNLLQCAHEFWGSS
CCCCHHHHHHHHHCCCCEEEEECHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
CADKRFLQVSTDEVYGSIENDSDYFIEDSNLMPNSPYSASKAGADMMVRAFGRTYGLPVI
CCCCCEEEEEHHHHEECCCCCCCEEEECCCCCCCCCCCCHHCCHHHHHHHHHHHCCHHHH
ITRCCNNYGPYQYSEKLIPICIIKALNDEPIPIYGDGTNIREWIHVSDHCSAIIKALFYG
HHHHHCCCCCCCCCCCCEEEEEEEECCCCCCEEEECCCCHHHHEEHHHHHHHHHHHHHCC
TPGEVYNIGSGEEVSNVDMAKIILSNLSKPTDAIKKVNDRLGHDRRYALDSSKIKNQLSW
CCCCEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHEECCHHHHHHHHHH
ACSYTLEEGIKETIEWYKNNQTWWND
HHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRVLLVTGGAGFIGSNFIRYFLETNKDFIVVNYDKLTYAGNLNNLKEVEMLPTYRFVKGD
CEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCEEEECCCCCCHHHHHCCCCCEEECC
ICDSEEFNRTLKKYNPDYIINFAAESHVDRSISGPSVFGQTNFMGTLNLLQCAHEFWGSS
CCCCHHHHHHHHHCCCCEEEEECHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
CADKRFLQVSTDEVYGSIENDSDYFIEDSNLMPNSPYSASKAGADMMVRAFGRTYGLPVI
CCCCCEEEEEHHHHEECCCCCCCEEEECCCCCCCCCCCCHHCCHHHHHHHHHHHCCHHHH
ITRCCNNYGPYQYSEKLIPICIIKALNDEPIPIYGDGTNIREWIHVSDHCSAIIKALFYG
HHHHHCCCCCCCCCCCCEEEEEEEECCCCCCEEEECCCCHHHHEEHHHHHHHHHHHHHCC
TPGEVYNIGSGEEVSNVDMAKIILSNLSKPTDAIKKVNDRLGHDRRYALDSSKIKNQLSW
CCCCEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHEECCHHHHHHHHHH
ACSYTLEEGIKETIEWYKNNQTWWND
HHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]