BacMap

Definition	Clostridium botulinum A2 str. Kyoto chromosome, complete genome.
Accession	NC_012563
Length	4,155,278

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The map label for this gene is 226949327

Identifier: 226949327

GI number: 226949327

Start: 2316733

End: 2318226

Strand: Reverse

Name: 226949327

Synonym: CLM_2248

Alternate gene names: NA

Gene position: 2318226-2316733 (Counterclockwise)

Preceding gene: 226949328

Following gene: 226949326

Centisome position: 55.79

GC content: 32.06

Gene sequence:

>1494_bases
ATGAAAAAATCCAGAGAACAATGGGGGACAAAAGCAGGGTTCCTACTTGCAGCTATTGGTTCTGCTGTAGGGCTAGGTAA
TATATGGAGATTTCCTTATGTAGCTTATTCAAATGGAGGTGGAGCTTTCTTAATTCCGTATTTTTTCGCTATATTCACAG
CGGGAATACCTCTATTAATTCTTGAATATGGAATGGGACATAAATTTAGAGGTTCAACACCACTTGCAATATCAAGAGCA
AATAAAAAATGGGAATGGCTTGGTTGGTGGCCTATAATTAGCGCAGTTATAATTTTATCCTATTATTCAATGATTTTGAG
TTTGTCTATGAAGTACTTAACATTAAGCTTTAAAAAAGTATGGGGAAATGACACTAACAGTTATTTTTATAATGAAGTAT
TAAAATTATCATCATCACCCTTTGATTTTGGAGGTATAATTGTTCCAATACTAATAGGAATAATTTTAATTTGGCTAATT
AATTGGTTTATATGTTATAAAGGAATAAAAGCTGGAATTGAAAAATTAAGCAAAATATTATTACCAGCTCTATTAATAAT
AATGATAATTATAGTAATAAAGGGAGTGACTCTAGAAGGTGCTTCTTTAGGCTTAAACACACTGTTTACACCTGATTGGC
AAAAAGTTAAAGATCCTAAAGTTTGGATTGCAGCATATGGACAAGTATTTTTCTCATTAAGTATAGCTACAGGAATTATG
ATGACTTATTCCAGCTATCTTCCTAAAAAAACAGATATAAATAATAGTGCATTCATGACTGCTTTTGCCAATTGTGGATT
TGAATTTTTATCTGCCATAGGGGTTTTTGCAATACTTGGATTCATGGCAACAACCCAAGGAGTATCTGTTGATAAAGTTG
CATCCGATGGTATAGGACTTGCATTTATAGCATTCCCTAAAGTATTCTCAGTAATGGGTACATGGGGAAACATACTATCT
GTATTATTCTTTACTTGCTTAATATTTGCAGGATTAACATCGGCAGTATCCTTAGTCGAAGCAATATCCTCAGCCATAAT
AGATAAAACAGGATGGGAACGTAAAAAAGTAGTAACAGGAATTTTGTTAGTCGGATTTATTATAAGTATCTCCTTTGCAA
CTAGAGCAGGATTATACCTATTGGATATTATGGATAACTTTATTAATAATTATGGAGTAGTTGTAGTTGGATTACTAGAA
ACTATTTTAGTGGGGTGGATTGTAAACCCTAAAACAATTCGTGACCATACAAATTCAGTATCCTATTATAGAATAGGAAA
ATGGTGGGATGTAGTTATTAAATGTATAAACCCAATTGTTTTAACTTTCATTCTAGTACAAAGTTTTATTACAGAAATGC
GTACGCCTTATGGTGGATATGATTCGCTGGCATTATTTGTTTATGGATGGGGAGCTATTTTAATAGGGATTACTGGATCA
ATCTTAATAAGTAAACAACCTTGGAGAAATAATATCAATTTAAAAAATGAATAA

Upstream 100 bases:

>100_bases
ATCCTAAGTAAAAAAGTCTCATAGTATAAACTGTGGGGCTTTTTTATTTATAAATATCAATATTTGGAAAATTCAGACGA
AATATTTAGGAGGAATAGAA

Downstream 100 bases:

>100_bases
AGGCGTGGTGATAAAAATGACATTAAGTGCAATGATATTCTTCACTATAGGTGCAACTGTACTTTGGGGAGGATTATTGA
CTACTATAATAATTTCAGTG

Product: sodium:neurotransmitter symporter family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MKKSREQWGTKAGFLLAAIGSAVGLGNIWRFPYVAYSNGGGAFLIPYFFAIFTAGIPLLILEYGMGHKFRGSTPLAISRA
NKKWEWLGWWPIISAVIILSYYSMILSLSMKYLTLSFKKVWGNDTNSYFYNEVLKLSSSPFDFGGIIVPILIGIILIWLI
NWFICYKGIKAGIEKLSKILLPALLIIMIIIVIKGVTLEGASLGLNTLFTPDWQKVKDPKVWIAAYGQVFFSLSIATGIM
MTYSSYLPKKTDINNSAFMTAFANCGFEFLSAIGVFAILGFMATTQGVSVDKVASDGIGLAFIAFPKVFSVMGTWGNILS
VLFFTCLIFAGLTSAVSLVEAISSAIIDKTGWERKKVVTGILLVGFIISISFATRAGLYLLDIMDNFINNYGVVVVGLLE
TILVGWIVNPKTIRDHTNSVSYYRIGKWWDVVIKCINPIVLTFILVQSFITEMRTPYGGYDSLALFVYGWGAILIGITGS
ILISKQPWRNNINLKNE

Sequences:

>Translated_497_residues
MKKSREQWGTKAGFLLAAIGSAVGLGNIWRFPYVAYSNGGGAFLIPYFFAIFTAGIPLLILEYGMGHKFRGSTPLAISRA
NKKWEWLGWWPIISAVIILSYYSMILSLSMKYLTLSFKKVWGNDTNSYFYNEVLKLSSSPFDFGGIIVPILIGIILIWLI
NWFICYKGIKAGIEKLSKILLPALLIIMIIIVIKGVTLEGASLGLNTLFTPDWQKVKDPKVWIAAYGQVFFSLSIATGIM
MTYSSYLPKKTDINNSAFMTAFANCGFEFLSAIGVFAILGFMATTQGVSVDKVASDGIGLAFIAFPKVFSVMGTWGNILS
VLFFTCLIFAGLTSAVSLVEAISSAIIDKTGWERKKVVTGILLVGFIISISFATRAGLYLLDIMDNFINNYGVVVVGLLE
TILVGWIVNPKTIRDHTNSVSYYRIGKWWDVVIKCINPIVLTFILVQSFITEMRTPYGGYDSLALFVYGWGAILIGITGS
ILISKQPWRNNINLKNE
>Mature_497_residues
MKKSREQWGTKAGFLLAAIGSAVGLGNIWRFPYVAYSNGGGAFLIPYFFAIFTAGIPLLILEYGMGHKFRGSTPLAISRA
NKKWEWLGWWPIISAVIILSYYSMILSLSMKYLTLSFKKVWGNDTNSYFYNEVLKLSSSPFDFGGIIVPILIGIILIWLI
NWFICYKGIKAGIEKLSKILLPALLIIMIIIVIKGVTLEGASLGLNTLFTPDWQKVKDPKVWIAAYGQVFFSLSIATGIM
MTYSSYLPKKTDINNSAFMTAFANCGFEFLSAIGVFAILGFMATTQGVSVDKVASDGIGLAFIAFPKVFSVMGTWGNILS
VLFFTCLIFAGLTSAVSLVEAISSAIIDKTGWERKKVVTGILLVGFIISISFATRAGLYLLDIMDNFINNYGVVVVGLLE
TILVGWIVNPKTIRDHTNSVSYYRIGKWWDVVIKCINPIVLTFILVQSFITEMRTPYGGYDSLALFVYGWGAILIGITGS
ILISKQPWRNNINLKNE

Specific function: Putative sodium-dependent transporter [H]

COG id: COG0733

COG function: function code R; Na+-dependent transporters of the SNF family

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family [H]

Homologues:

Organism=Homo sapiens, GI289191351, Length=498, Percent_Identity=29.9196787148594, Blast_Score=214, Evalue=2e-55,
Organism=Homo sapiens, GI4557046, Length=498, Percent_Identity=29.9196787148594, Blast_Score=214, Evalue=2e-55,
Organism=Homo sapiens, GI289191377, Length=498, Percent_Identity=29.9196787148594, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI21361581, Length=488, Percent_Identity=28.2786885245902, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI4507043, Length=505, Percent_Identity=30.0990099009901, Blast_Score=208, Evalue=9e-54,
Organism=Homo sapiens, GI7657587, Length=461, Percent_Identity=29.7180043383948, Blast_Score=207, Evalue=3e-53,
Organism=Homo sapiens, GI67782317, Length=508, Percent_Identity=29.5275590551181, Blast_Score=203, Evalue=3e-52,
Organism=Homo sapiens, GI67782313, Length=508, Percent_Identity=29.5275590551181, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI67782315, Length=508, Percent_Identity=29.5275590551181, Blast_Score=202, Evalue=5e-52,
Organism=Homo sapiens, GI171184408, Length=462, Percent_Identity=30.7359307359307, Blast_Score=202, Evalue=6e-52,
Organism=Homo sapiens, GI171184406, Length=462, Percent_Identity=30.7359307359307, Blast_Score=202, Evalue=6e-52,
Organism=Homo sapiens, GI171184404, Length=462, Percent_Identity=30.7359307359307, Blast_Score=202, Evalue=6e-52,
Organism=Homo sapiens, GI92859670, Length=525, Percent_Identity=28, Blast_Score=201, Evalue=2e-51,
Organism=Homo sapiens, GI4507041, Length=497, Percent_Identity=28.7726358148893, Blast_Score=195, Evalue=1e-49,
Organism=Homo sapiens, GI197276623, Length=489, Percent_Identity=29.2433537832311, Blast_Score=186, Evalue=4e-47,
Organism=Homo sapiens, GI54607094, Length=489, Percent_Identity=29.2433537832311, Blast_Score=186, Evalue=4e-47,
Organism=Homo sapiens, GI134304856, Length=500, Percent_Identity=28.4, Blast_Score=183, Evalue=4e-46,
Organism=Homo sapiens, GI194018524, Length=524, Percent_Identity=28.8167938931298, Blast_Score=182, Evalue=6e-46,
Organism=Homo sapiens, GI188528618, Length=488, Percent_Identity=26.0245901639344, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI51468073, Length=528, Percent_Identity=26.5151515151515, Blast_Score=172, Evalue=7e-43,
Organism=Homo sapiens, GI5032097, Length=497, Percent_Identity=28.5714285714286, Blast_Score=165, Evalue=9e-41,
Organism=Homo sapiens, GI218563756, Length=497, Percent_Identity=28.3702213279678, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI11181770, Length=541, Percent_Identity=26.0628465804067, Blast_Score=152, Evalue=9e-37,
Organism=Homo sapiens, GI300360519, Length=334, Percent_Identity=27.5449101796407, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI289191353, Length=330, Percent_Identity=28.4848484848485, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI58219014, Length=305, Percent_Identity=30.8196721311475, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI33354281, Length=309, Percent_Identity=30.7443365695793, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI6005715, Length=304, Percent_Identity=31.25, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI12408638, Length=536, Percent_Identity=23.6940298507463, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI218563758, Length=333, Percent_Identity=28.5285285285285, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI21361693, Length=119, Percent_Identity=34.453781512605, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI197276625, Length=127, Percent_Identity=37.007874015748, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI55749589, Length=303, Percent_Identity=25.7425742574257, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI226437581, Length=255, Percent_Identity=27.843137254902, Blast_Score=76, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17555248, Length=511, Percent_Identity=28.9628180039139, Blast_Score=215, Evalue=4e-56,
Organism=Caenorhabditis elegans, GI71995588, Length=511, Percent_Identity=26.2230919765166, Blast_Score=190, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17554804, Length=474, Percent_Identity=29.746835443038, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17535731, Length=555, Percent_Identity=26.6666666666667, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI133952791, Length=505, Percent_Identity=28.3168316831683, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI71998244, Length=401, Percent_Identity=30.1745635910224, Blast_Score=154, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI25146584, Length=462, Percent_Identity=26.6233766233766, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17569475, Length=452, Percent_Identity=26.3274336283186, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI115534083, Length=510, Percent_Identity=22.3529411764706, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI212642127, Length=339, Percent_Identity=27.7286135693215, Blast_Score=112, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17542050, Length=287, Percent_Identity=28.5714285714286, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17542048, Length=385, Percent_Identity=21.5584415584416, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24654186, Length=531, Percent_Identity=28.060263653484, Blast_Score=216, Evalue=3e-56,
Organism=Drosophila melanogaster, GI24762620, Length=484, Percent_Identity=30.7851239669421, Blast_Score=212, Evalue=6e-55,
Organism=Drosophila melanogaster, GI221329674, Length=501, Percent_Identity=30.3393213572854, Blast_Score=211, Evalue=7e-55,
Organism=Drosophila melanogaster, GI24639514, Length=501, Percent_Identity=30.3393213572854, Blast_Score=211, Evalue=9e-55,
Organism=Drosophila melanogaster, GI281364136, Length=522, Percent_Identity=29.8850574712644, Blast_Score=211, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24639516, Length=521, Percent_Identity=29.3666026871401, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI24655079, Length=539, Percent_Identity=30.6122448979592, Blast_Score=202, Evalue=6e-52,
Organism=Drosophila melanogaster, GI24661428, Length=505, Percent_Identity=27.3267326732673, Blast_Score=197, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24582567, Length=470, Percent_Identity=28.7234042553192, Blast_Score=196, Evalue=3e-50,
Organism=Drosophila melanogaster, GI19921936, Length=536, Percent_Identity=28.9179104477612, Blast_Score=196, Evalue=4e-50,
Organism=Drosophila melanogaster, GI281365034, Length=495, Percent_Identity=29.0909090909091, Blast_Score=194, Evalue=2e-49,
Organism=Drosophila melanogaster, GI17136952, Length=479, Percent_Identity=27.7661795407098, Blast_Score=191, Evalue=1e-48,
Organism=Drosophila melanogaster, GI281363702, Length=497, Percent_Identity=28.3702213279678, Blast_Score=190, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24639853, Length=543, Percent_Identity=28.3609576427256, Blast_Score=189, Evalue=3e-48,
Organism=Drosophila melanogaster, GI281364394, Length=479, Percent_Identity=27.7661795407098, Blast_Score=189, Evalue=4e-48,
Organism=Drosophila melanogaster, GI28574704, Length=479, Percent_Identity=27.7661795407098, Blast_Score=189, Evalue=4e-48,
Organism=Drosophila melanogaster, GI17136950, Length=479, Percent_Identity=27.7661795407098, Blast_Score=189, Evalue=4e-48,
Organism=Drosophila melanogaster, GI28574702, Length=479, Percent_Identity=27.7661795407098, Blast_Score=189, Evalue=4e-48,
Organism=Drosophila melanogaster, GI19921352, Length=507, Percent_Identity=27.810650887574, Blast_Score=184, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24584353, Length=507, Percent_Identity=27.810650887574, Blast_Score=184, Evalue=2e-46,
Organism=Drosophila melanogaster, GI45551088, Length=523, Percent_Identity=25.8126195028681, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24652942, Length=526, Percent_Identity=25.6653992395437, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI45550877, Length=496, Percent_Identity=25.2016129032258, Blast_Score=170, Evalue=3e-42,
Organism=Drosophila melanogaster, GI45551183, Length=398, Percent_Identity=27.1356783919598, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24582569, Length=440, Percent_Identity=27.0454545454545, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI281363700, Length=338, Percent_Identity=30.7692307692308, Blast_Score=146, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000175 [H]

Pfam domain/function: PF00209 SNF [H]

EC number: NA

Molecular weight: Translated: 54971; Mature: 54971

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS00610 NA_NEUROTRAN_SYMP_1 ; PS50267 NA_NEUROTRAN_SYMP_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSREQWGTKAGFLLAAIGSAVGLGNIWRFPYVAYSNGGGAFLIPYFFAIFTAGIPLLI
CCCCHHHHCCHHHHHHHHHHHHHCCCCHHCCCEEEEECCCCEEHHHHHHHHHHCCHHHHH
LEYGMGHKFRGSTPLAISRANKKWEWLGWWPIISAVIILSYYSMILSLSMKYLTLSFKKV
HHHCCCCCCCCCCCEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WGNDTNSYFYNEVLKLSSSPFDFGGIIVPILIGIILIWLINWFICYKGIKAGIEKLSKIL
HCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPALLIIMIIIVIKGVTLEGASLGLNTLFTPDWQKVKDPKVWIAAYGQVFFSLSIATGIM
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHH
MTYSSYLPKKTDINNSAFMTAFANCGFEFLSAIGVFAILGFMATTQGVSVDKVASDGIGL
HHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCC
AFIAFPKVFSVMGTWGNILSVLFFTCLIFAGLTSAVSLVEAISSAIIDKTGWERKKVVTG
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ILLVGFIISISFATRAGLYLLDIMDNFINNYGVVVVGLLETILVGWIVNPKTIRDHTNSV
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCC
SYYRIGKWWDVVIKCINPIVLTFILVQSFITEMRTPYGGYDSLALFVYGWGAILIGITGS
CEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC
ILISKQPWRNNINLKNE
EEEECCCCCCCCCCCCC
>Mature Secondary Structure
MKKSREQWGTKAGFLLAAIGSAVGLGNIWRFPYVAYSNGGGAFLIPYFFAIFTAGIPLLI
CCCCHHHHCCHHHHHHHHHHHHHCCCCHHCCCEEEEECCCCEEHHHHHHHHHHCCHHHHH
LEYGMGHKFRGSTPLAISRANKKWEWLGWWPIISAVIILSYYSMILSLSMKYLTLSFKKV
HHHCCCCCCCCCCCEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WGNDTNSYFYNEVLKLSSSPFDFGGIIVPILIGIILIWLINWFICYKGIKAGIEKLSKIL
HCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPALLIIMIIIVIKGVTLEGASLGLNTLFTPDWQKVKDPKVWIAAYGQVFFSLSIATGIM
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHH
MTYSSYLPKKTDINNSAFMTAFANCGFEFLSAIGVFAILGFMATTQGVSVDKVASDGIGL
HHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCC
AFIAFPKVFSVMGTWGNILSVLFFTCLIFAGLTSAVSLVEAISSAIIDKTGWERKKVVTG
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
ILLVGFIISISFATRAGLYLLDIMDNFINNYGVVVVGLLETILVGWIVNPKTIRDHTNSV
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCC
SYYRIGKWWDVVIKCINPIVLTFILVQSFITEMRTPYGGYDSLALFVYGWGAILIGITGS
CEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCC
ILISKQPWRNNINLKNE
EEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]