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The map label for this gene is miaA [H]

Identifier: 226949039

GI number: 226949039

Start: 2013015

End: 2013950

Strand: Reverse

Name: miaA [H]

Synonym: CLM_1955

Alternate gene names: 226949039

Gene position: 2013950-2013015 (Counterclockwise)

Preceding gene: 226949040

Following gene: 226949038

Centisome position: 48.47

GC content: 29.38

Gene sequence:

>936_bases
ATGATAGATTTATTAATAATTGCAGGTCCTACGGCAGTAGGGAAAACAGATATTTCCATAGAGCTTGCAGAAAAACTAAA
TGGAGAGATAATATCCGCTGACTCTATGCAAATTTATAAATACATGGATATAGGTTCTGCCAAAATAACTAAGGATGAAA
TGAAGGGGATACCTCACCATCTTATAGATGTAGTAGAACCCCATGAAGAATTCAATGTATCTTCCTTTAAAGCTTTAGCA
GAGAAATCTATAAAAGATATATGGAACAAAGGGAAGTTGCCCATAATAGTAGGAGGTACAGGGTTATATATTAATTCTTT
AATCTACAATTATGATTTTACGGATGCAGATAGAGACGAAAAATATAGAGAATATCTTACTAAATTAGCAGAAGATAAAG
GGAAGGAATATGTTCATAGCCTGCTAAAAGATATAGATGAAGAATCCTATGAAAAGTTATATCCTAATGACCTAAAAAGA
GTAGTAAGAGCCTTAGAAGTTTACAAAATCACAGGTAAATCTATAAGCGAATACACAAAAGAAAACGAGAAAAAATTATA
TGATATCCCTTATAATGTAAACTACTTTATTTTAAATATGAATAGAGAGGTACTCTATGAAAGAATTAATAAAAGAGTGG
ATATAATGATGAGTAAAGGCTTAATAGAAGAAGTAAAAAAATTAGAGTCCATGGGATATACTCCAGATATGCAATCTATG
AAAGGTATAGGTTATAAAGAAGTTTTATTCTATTTAAATGGAGATATTTCACTAGATGAAGCTATTTATTTAATAAAAAA
AGGAAGCAGAAATTATGCTAAAAGACAACTTACTTGGTTTAGAAAGGATAAAAGATCTATATGGATAGATAAAGATAAAT
ATAGCTCTGAGGAGGAAATAGTAGATAAAATTATAAAAATGGTGAAAGATAAATAG

Upstream 100 bases:

>100_bases
ATCCATATACTTGTCCCCATGGAAGACCTACTATGATTAAATTTACATTAAAAGATTTAGAAAAAAAATTTAAAAGAATA
CAATAAGGGGGATAAGCAAA

Downstream 100 bases:

>100_bases
AATTAAAAGGATTTTTAATTTATTTATAGAATTATATAAGAATAAGTTTCTAATTATTTGTAATTTTTTAGGAGGGAAAG
GTTATATGACTAAAGTTGTT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MIDLLIIAGPTAVGKTDISIELAEKLNGEIISADSMQIYKYMDIGSAKITKDEMKGIPHHLIDVVEPHEEFNVSSFKALA
EKSIKDIWNKGKLPIIVGGTGLYINSLIYNYDFTDADRDEKYREYLTKLAEDKGKEYVHSLLKDIDEESYEKLYPNDLKR
VVRALEVYKITGKSISEYTKENEKKLYDIPYNVNYFILNMNREVLYERINKRVDIMMSKGLIEEVKKLESMGYTPDMQSM
KGIGYKEVLFYLNGDISLDEAIYLIKKGSRNYAKRQLTWFRKDKRSIWIDKDKYSSEEEIVDKIIKMVKDK

Sequences:

>Translated_311_residues
MIDLLIIAGPTAVGKTDISIELAEKLNGEIISADSMQIYKYMDIGSAKITKDEMKGIPHHLIDVVEPHEEFNVSSFKALA
EKSIKDIWNKGKLPIIVGGTGLYINSLIYNYDFTDADRDEKYREYLTKLAEDKGKEYVHSLLKDIDEESYEKLYPNDLKR
VVRALEVYKITGKSISEYTKENEKKLYDIPYNVNYFILNMNREVLYERINKRVDIMMSKGLIEEVKKLESMGYTPDMQSM
KGIGYKEVLFYLNGDISLDEAIYLIKKGSRNYAKRQLTWFRKDKRSIWIDKDKYSSEEEIVDKIIKMVKDK
>Mature_311_residues
MIDLLIIAGPTAVGKTDISIELAEKLNGEIISADSMQIYKYMDIGSAKITKDEMKGIPHHLIDVVEPHEEFNVSSFKALA
EKSIKDIWNKGKLPIIVGGTGLYINSLIYNYDFTDADRDEKYREYLTKLAEDKGKEYVHSLLKDIDEESYEKLYPNDLKR
VVRALEVYKITGKSISEYTKENEKKLYDIPYNVNYFILNMNREVLYERINKRVDIMMSKGLIEEVKKLESMGYTPDMQSM
KGIGYKEVLFYLNGDISLDEAIYLIKKGSRNYAKRQLTWFRKDKRSIWIDKDKYSSEEEIVDKIIKMVKDK

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=311, Percent_Identity=31.8327974276527, Blast_Score=147, Evalue=8e-36,
Organism=Escherichia coli, GI1790613, Length=306, Percent_Identity=35.9477124183007, Blast_Score=209, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI25144712, Length=274, Percent_Identity=33.5766423357664, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6324848, Length=292, Percent_Identity=35.6164383561644, Blast_Score=172, Evalue=7e-44,
Organism=Drosophila melanogaster, GI24649830, Length=267, Percent_Identity=35.2059925093633, Blast_Score=165, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 36234; Mature: 36234

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDLLIIAGPTAVGKTDISIELAEKLNGEIISADSMQIYKYMDIGSAKITKDEMKGIPHH
CEEEEEEECCCCCCCCCCEEEEHHHHCCCEEECCCEEEEEEEECCCCEECHHHHHCCCHH
LIDVVEPHEEFNVSSFKALAEKSIKDIWNKGKLPIIVGGTGLYINSLIYNYDFTDADRDE
HHHHHCCHHHCCHHHHHHHHHHHHHHHHCCCCCEEEECCCHHEEHHHHEECCCCCCCCHH
KYREYLTKLAEDKGKEYVHSLLKDIDEESYEKLYPNDLKRVVRALEVYKITGKSISEYTK
HHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHH
ENEKKLYDIPYNVNYFILNMNREVLYERINKRVDIMMSKGLIEEVKKLESMGYTPDMQSM
HCCCEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
KGIGYKEVLFYLNGDISLDEAIYLIKKGSRNYAKRQLTWFRKDKRSIWIDKDKYSSEEEI
HCCCHHHEEEEECCCCCHHHHEEEEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHH
VDKIIKMVKDK
HHHHHHHHCCC
>Mature Secondary Structure
MIDLLIIAGPTAVGKTDISIELAEKLNGEIISADSMQIYKYMDIGSAKITKDEMKGIPHH
CEEEEEEECCCCCCCCCCEEEEHHHHCCCEEECCCEEEEEEEECCCCEECHHHHHCCCHH
LIDVVEPHEEFNVSSFKALAEKSIKDIWNKGKLPIIVGGTGLYINSLIYNYDFTDADRDE
HHHHHCCHHHCCHHHHHHHHHHHHHHHHCCCCCEEEECCCHHEEHHHHEECCCCCCCCHH
KYREYLTKLAEDKGKEYVHSLLKDIDEESYEKLYPNDLKRVVRALEVYKITGKSISEYTK
HHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHH
ENEKKLYDIPYNVNYFILNMNREVLYERINKRVDIMMSKGLIEEVKKLESMGYTPDMQSM
HCCCEEEECCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
KGIGYKEVLFYLNGDISLDEAIYLIKKGSRNYAKRQLTWFRKDKRSIWIDKDKYSSEEEI
HCCCHHHEEEEECCCCCHHHHEEEEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHH
VDKIIKMVKDK
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA